Navigation Links
New tool isolates RNA within specific cells
Date:5/18/2009

r 'listening' to host cells before and after the initiation of a disease to determine how cells respond, or, for example study healthy immune cells versus bacterially-challenged immune cells or neurons before they learn a task and after they learn a task to determine what changes in the cell are caused by the experience.

The new UO-developed tool builds on work led by co-author Michael D. Cleary, who as a doctoral student at Stanford University unveiled the T. gondii-based approach for use in analyzing RNA synthesis and decay in 2005 in Nature Biotechnology. Cleary, now a faculty member at the University of California, Merced, worked on the UO project as a postdoctoral fellowship funded by the National Institutes of Health and HHMI.

Cleary's group built its tool with the enzyme uracil phosphoribosyltransferase (UPRT), a nucleotide salvage enzyme that prepares nucleotides for incorporation into newly synthesized RNA. By altering the nucleotide analog 4-thiouracil, the UPRT enzyme caused RNA to become tagged with thiouracil (TU), allowing the "TU-tagged" RNA to be purified from untagged RNA.

In Doe's lab, Miller, Cleary and research technician Kristin J. Robinson of the UO's institutes of Neuroscience and Molecular Biology manipulated Drosophila so that they would only express UPRT in specific target cells. The group tested the new approach in embryos, larvae and adults using microarray technology to detect cell type-specific gene expression. The researchers say the method should work in other systems, including vertebrates, by using gene transfer, retroviral delivery, electrical pulses of molecules through cell membranes, or messenger RNA injection.


'/>"/>

Contact: Jim Barlow
jebarlow@uoregon.edu
541-346-3481
University of Oregon
Source:Eurekalert  

Page: 1 2

Related biology news :

1. CCNY, CSHL biologists find birdsong of isolates reverts to norm over several generations
2. Scientists decode genomes of diverse TB isolates
3. TECNALIA leads five projects within the VII Framework Program
4. Gender biases in leadership selection during competitions within and between groups
5. Surface tension drives segregation within cell mixtures
6. NIH awards Argonne $800,000 to develop tool to measure distances within proteins
7. Study finds healthy intestinal bacteria within chicken eggs
8. Integrating restoration and conservation within the ecosystem approach
9. New treatment for age-related macular degeneration within sight
10. M2SYS Technology Partners with TeraCorp Enterprises to Provide Added Security Within Check Cashing Solution
11. CO2 emissions could violate EPA ocean-quality standards within decades
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
New tool isolates RNA within specific cells
(Date:7/23/2014)... State University shows that urban "heat islands" are slowly ... factor is that researchers have found warmer temperatures increase ... insect a significant tree pest by 300 ... adult gloomy scales on urban trees. , "We,d been ... scale in cities, and now we know why," says ...
(Date:7/23/2014)... lab experiments using tissue samples cultured from cystic fibrosis ... the UNC Marsico Lung Institute have shown that a ... of another CF drug. , The finding, published today ... how a mutant CFTR protein becomes unstable and loses ... of the two drugs. The research offers several insights ...
(Date:7/23/2014)... drought that has the entire country in its grip is ... also be responsible for the proliferation of a heat-loving amoeba ... and hot springs, which the drought has made warmer than ... of an infection caused by this parasite after swimming in ... nose of an individual and travels to the brain. Nose ...
Breaking Biology News(10 mins):Urban heat boosts some pest populations 200-fold, killing red maples 2Urban heat boosts some pest populations 200-fold, killing red maples 3UNC researchers find unsuspected characteristics of new CF drugs, offering potential paths to more effective therapies 2UNC researchers find unsuspected characteristics of new CF drugs, offering potential paths to more effective therapies 3UNC researchers find unsuspected characteristics of new CF drugs, offering potential paths to more effective therapies 4Diseases of another kind 2
... Penn State researchers have found that native pollinators, ... same viral diseases as honey bees and that these ... on December 22nd in PLoS ONE, an online open-access ... medical research. This multi-institutional study provides new insights ...
... Researchers at the University of Minnesota have discovered ... antiviral protein called APOBEC3F. The results suggest that a ... effective antiviral agent and that shielding of a common ... outcome. This discovery highlights the potential for a ...
... can scientists learn from watching a group of people ... new friends? Quite a lot, says University of Melbourne, ... of group in a fatigue management study he and ... the Acoustical Society of America. Their report shows ...
Cached Biology News:Movement and threat of RNA viruses widespread in pollinator community 2University of Minnesota discovery suggests a new way to prevent HIV from infecting human cells 2Measuring fatigue through the voice 2
(Date:7/23/2014)... 2014 Last year, Formedix ... leading global provider of data management solutions for clinical ... leading-edge system for electronic data capture (EDC) and clinical ... and study execution tools added TrialMaster to its Transform ... version of Transform for TrialMaster, is being released this ...
(Date:7/23/2014)... , 23 de julio de 2014 ... la Compañía) ha anunciado hoy una asociación con ... (ABIA o el Instituto), para el propósito ... dispositivo de terapia de señal bioeléctrica (el E-QURE ... El Instituto, que tiene una importante experiencia en ...
(Date:7/23/2014)... TEL AVIV, Israel , July 23, 2014 ... Ltd ., a portfolio company of Trendlines ... with one of Israel,s ... (Logo: http://photos.prnewswire.com/prnh/20140121/666259-a) , Valentis,s technology combines ... from plant pulp waste, with additional nanoparticles to ...
(Date:7/23/2014)... - BioAmber Inc. (NYSE: BIOA ), ... that the underwriters of its common stock offering ... additional 420,000 shares of common stock at the ... underwriting discounts and commissions, in connection with its ... of common stock, bringing the expected net proceeds ...
Breaking Biology Technology:Formedix and OmniComm Systems® Partnership Strengthened with the Release of Enhanced TrialMaster® Integration for EDC Build 2Formedix and OmniComm Systems® Partnership Strengthened with the Release of Enhanced TrialMaster® Integration for EDC Build 3ADB International Group Inc. anuncia una asociación con Austen BioInnovation Institute 2ADB International Group Inc. anuncia una asociación con Austen BioInnovation Institute 3ADB International Group Inc. anuncia una asociación con Austen BioInnovation Institute 4ADB International Group Inc. anuncia una asociación con Austen BioInnovation Institute 5ADB International Group Inc. anuncia una asociación con Austen BioInnovation Institute 6Valentis Nanotech Signs MOU to Integrate Technology in Thermoplastics Production 2BioAmber announces exercise in full of underwriters' option in connection with previously priced follow-on offering 2BioAmber announces exercise in full of underwriters' option in connection with previously priced follow-on offering 3
... Quarter ... Salesforce ... Insulet Corporation,(Nasdaq: PODD ), a leader in wearable insulin ... for,the first quarter ended March 31, 2008., First quarter 2008 ...
... 13 Hospira, Inc.,(NYSE: HSP ), a ... that Barbara L. Bowles, CFA, has been elected ... investment,management and finance expertise from leadership positions held ... advisory,firms. The addition of Bowles expands Hospira,s board ...
... Angiotech Pharmaceuticals,Inc. (NASDAQ: ANPI , TSX: ANP), ... announced the appointment of Dr. Hank,McKinnell to its ... former Chief Executive Officer and former Chairman,of the ... currently Chairman of the,Accordia Global Health Foundation, an ...
Cached Biology Technology:Insulet Reports First Quarter 2008 Results 2Insulet Reports First Quarter 2008 Results 3Insulet Reports First Quarter 2008 Results 4Insulet Reports First Quarter 2008 Results 5Insulet Reports First Quarter 2008 Results 6Insulet Reports First Quarter 2008 Results 7Hospira Expands Board of Directors 2Angiotech appoints Hank McKinnell to its Board of Directors 2
... bath, with a comprehensive array of accessories. ... baths with seamless, splash-water protected keypad offer ... (LED) indicating up to 6 temperature values ... MICROPROCESSOR technology with PID temperature control eliminates ...
... Provides a buffered anti-freeze environment for ... Maintains a liquid, low viscosity solution at ... is necessary and sampling is convenient ... during long-term storage Protect enzyme conjugates ...
This buffer is Specifically formulated to be used with Endogen's Poly-HRP Streptavidin...
... SearchLight® Array Analyst Software has been ... arrays. Mathematical algorithms allow the software to ... spot within each well of an ELISA ... SearchLight® Array Analyst Software has been customized ...
Biology Products: