Navigation Links
New tool for mining bacterial genome for novel drugs
Date:1/25/2013

ailed look yet at what happens when microbes develop drug resistance. Bachmann is an expert in natural products drug discovery and McLean, an assistant professor of chemistry, is a pioneer in the development of analytical instrumentation and chemical techniques that can identify thousands of different biological compounds simultaneously, such as ion mobility-mass spectrometry.

"One of the daunting challenges is to rapidly inventory the tens to hundreds of thousands of molecules the bacteria construct to live, and then to read this inventory to understand how the bacteria compensate for their changing circumstances. To complicate matters further, we are looking for new drug-like molecules, so by definition we are looking for something that has not been seen before," said McLean.

Working with Research Assistant Dagmara Derewacz and graduate students Cody Goodwin and Ruth McNees, Bachmann and McLean started with the well-characterized soil bacterium Nocardiopsis. They exposed the bacterium to two different antibiotics streptomycin and rifampicin and observed the results.

"The first thing that happens is almost all of the bacteria die. Less than one cell in a million survives," said Bachmann.

The chemists then cultured the survivors (six streptomycin-resistant strains and five rifampicin-resistant strains) without the antibiotic and used McLean's instrumental methods to profile the drug-like compounds that they produced.

They discovered that the differences were much greater than they expected. The survivors had undergone extensive mutations, not only in the genes that produce secondary metabolites but also in the housekeeping genes that alter the way they make RNA and proteins. As a result, they determined that the resistant strains produced more than 300 compounds that were not expressed by the original organism.

"The cells appear to be 'de-repressing' as many of their silent genes as possible. This seems
'/>"/>

Contact: David Salisbury
david.salisbury@vanderbilt.edu
615-343-6803
Vanderbilt University
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Supplements and cows milk play biggest roles in determining vitamin D levels in children
2. Mining ancient ores for clues to early life
3. Minimizing mining damage with manure
4. Mining cleanup benefits from Texas A&M expertise
5. Study: Odd biochemistry yields lethal bacterial protein
6. Antibacterial agent used in common soaps found in increasing amounts in freshwater lakes
7. Stopping smoking reduces risk of bacterial pneumonia in people with HIV
8. Study quantifies the size of holes antibacterials create in cell walls to kill bacteria
9. RIT scientists decode 3 bacterial strains common to grapevines and sugarcane
10. OU study suggests the bacterial ecology that lives on humans has changed in the last 100 years
11. New edition of Bacterial Genetics text available
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:8/18/2015)... DUBLIN , Aug. 17, 2015 ... the addition of the "Global Biometric Authentication ... Application - Estimation & Forecast, 2015-2020" report ... biometric authentication and identification systems market is expected ... from 2015 to 2020 and generate over $25 ...
(Date:8/12/2015)... , August 12, 2015 As ... mobile payment innovation and advanced biometrics technology is accelerating ... way consumers rely on using their credit cards, the ... the future for payment services led by companies in ... ), :  Google, Inc. (NASDAQ: GOOG ), Apple ...
(Date:8/11/2015)... , August 11, 2015 Today, ZUK announced ... received as well as expected revenues in 2015 that relate to ... communicated revenue guidance of approximately 2,200 MSEK for 2015. ... a prominent smartphone manufacturer in ... selected FPC1 155 for ...
Breaking Biology News(10 mins):Global Biometric Authentication & Identification Market 2015: Focus On Modality, Motility & Application - Estimation & Forecasts to 2020 for the $25 Billion Industry 2Digital Wallet Devices & Mobile Payment Management Services Gain a Distinct Advantage in the Digital Payment Evolution as Demand Continues to Grow 2Digital Wallet Devices & Mobile Payment Management Services Gain a Distinct Advantage in the Digital Payment Evolution as Demand Continues to Grow 3Digital Wallet Devices & Mobile Payment Management Services Gain a Distinct Advantage in the Digital Payment Evolution as Demand Continues to Grow 4Digital Wallet Devices & Mobile Payment Management Services Gain a Distinct Advantage in the Digital Payment Evolution as Demand Continues to Grow 5Digital Wallet Devices & Mobile Payment Management Services Gain a Distinct Advantage in the Digital Payment Evolution as Demand Continues to Grow 6FPC's Touch Fingerprint Sensor FPC1155 in ZUK's Smartphone Z1 2
... field trials involving five major food crops grown ... harvesting dramatically less bounty than those raised in ... scientists warn that global food supplies could be ... The new findings are based on on-going open-air ...
... characterized by a decrease in bone mass and density ... and deformities, afflicts some 10 million Americans over the ... Public Health (HSPH) have discovered that eliminating a protein, ... bone mass throughout their skeletal system. The results may ...
... has identified a molecule that plays a key role ... of the adult brain. , QBI neural migration ... research provided new clues regarding development of the corpus ... between the left and right hemispheres of the adult ...
Cached Biology News:Food-crop yields in future greenhouse-gas conditions lower than expected 2Research identifies protein in mice that regulates bone formation 2
(Date:8/28/2015)... ... August 28, 2015 , ... Proove ... at the International Spine Intervention Society’ s 23rd Annual Scientific Meeting ... 1st, 2015. With a focus on evidence-based research and clinical application, the event ...
(Date:8/28/2015)... (PRWEB) , ... August 28, 2015 , ... Since the initial launch of FireflySci Inc. ... quirky humor and excellent customer service. Images such as the Fonz in a lab ... add some flavor to the humdrum spectroscopy field. , FireflySci is proud to ...
(Date:8/27/2015)... 27, 2015 /PRNewswire/ - Portage Biotech Inc. ("Portage") ... Biohaven Pharmaceutical Holding Company Limited (Biohaven), announced today ... study of BHV-0223, a glutamate modulating agent. Biohaven ... and recently obtained clearance from the U.S. Food ... testing. Portage holds 54% equity interest in Biohaven, ...
(Date:8/27/2015)... SAN DIEGO , Aug. 27, 2015 ... second annual meeting of the Japan Society of HTLV-1, ... Science Institute Auditorium. The purpose of the Society is ... well as the development of medical technology and research ... health and welfare. "HUYA is proud to ...
Breaking Biology Technology:Proove Biosciences Presents New Research Study at the International Spine Intervention Society’s Annual Scientific Meeting 2Proove Biosciences Presents New Research Study at the International Spine Intervention Society’s Annual Scientific Meeting 3Proove Biosciences Presents New Research Study at the International Spine Intervention Society’s Annual Scientific Meeting 4Cuvette Manufacturer FireflySci Launches Back to School Deals with Complimentary Shipping On All Orders 2Portage's Biohaven doses first human subject in Phase 1 trial with lead drug candidate BHV-0223 2Portage's Biohaven doses first human subject in Phase 1 trial with lead drug candidate BHV-0223 3Portage's Biohaven doses first human subject in Phase 1 trial with lead drug candidate BHV-0223 4HUYA Bioscience International Sponsors Japan Society Of HTLV-1 Annual Meeting 2HUYA Bioscience International Sponsors Japan Society Of HTLV-1 Annual Meeting 3HUYA Bioscience International Sponsors Japan Society Of HTLV-1 Annual Meeting 4
... unveiled a program of healthcare savings accounts, leveraging ... administration. , ,Spurred on by HSA authorization ... set of services which includes managing tax-advantaged healthcare ... in healthcare transactions. , ,"Our financial institution ...
... At the recent Digital Healthcare Conference top healthcare ... can improve patient safety, decrease medical costs, and ... Entrepreneurs Conference held in Milwaukee, Mithridion won the ... recent report has stated that Wisconsin lags behind ...
... their top issues, they dont see where security really fits ... few executives know about in most organizations: Security is ... scans and firewalls. Security should be at an executive level ... , ,In several semesters of network security classes, attendees from ...
Cached Biology Technology:To find solid security candidates, look beyond tech certificates 2To find solid security candidates, look beyond tech certificates 3To find solid security candidates, look beyond tech certificates 4
Secure-Seal™ spacer, one well, 20 mm diameter, 0.12 mm deep *set of 100*...
Rat Antibody to Mouse TCR VB8.1,8.2:FITC Recognises the V beta 8.1 and V beta 8.2 determinants of the murine T cell receptor. Specificity: mouse TCR VB8.1 and 8.2...
KAI 1 (G-2)...
Purified Mouse anti-Hu p120 Catenin (pT916) Storage Temperature: Refrigerate(2 to 8C)...
Biology Products: