Navigation Links
New tool for RNA silencing
Date:4/1/2010

Anti-sense reagents have been developed for C. Elegans micro RNA. Researchers writing in BioMed Central's open access journal Silence have created the first class of reagents to potently and selectively inhibit miRNAs in this widely used model organism.

Wen-hong Li, from the University of Texas Southwestern Medical Center, USA, worked with a team of researchers including Dr. Genhua Zheng and Dr. Victor Ambros (University of Massachusetts Medical School) to develop this latest addition to the genetics toolkit. He said, "Caenorhabditis elegans has long been used as a model organism for studying the regulation and function of small non-coding RNA molecules, and yet no antisense reagents have been available to reliably inhibit miRNAs in worms. Our fluorescently labeled reagents were synthesized by conjugating dextran with 2'-O-methyl oligoribonucleotide, and can be conveniently introduced into the germline of adult hermaphrodites and are transmitted to their progeny".

Li's team found that their new reagents efficiently and specifically inhibited targeted miRNA in different tissues, including the hypodermis, the vulva and the nervous system. They can be used combinatorially to inhibit more than one miRNA in the same animal. They conclude, "Combined with numerous mutants or reporter stains available, these reagents should provide a convenient approach to examine genetic interactions that involve miRNA, and may facilitate studying functions of miRNAs, especially ones whose deletion strains are difficult to generate. Further, the remarkable efficacy of these antisense reagents seen in worms also suggested C. elegans as a powerful, convenient, and economical biological system to facilitate developing new chemistry and novel probes for studying miRNA and other small non-coding RNAs".


'/>"/>

Contact: Graeme Baldwin
graeme.baldwin@biomedcentral.com
44-020-319-22165
BioMed Central
Source:Eurekalert

Page: 1

Related biology news :

1. Class of antibiotics can enhance gene-silencing tool
2. Cold Spring Harbor Protocols highlights gene silencing, cancer cell biology methods
3. New gene-silencing pathway found in plants
4. Researchers solve piece of large-scale gene silencing mystery
5. In lung cancer, silencing one crucial gene disrupts normal functioning of genome
6. A new gene silencing platform -- silence is golden
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/20/2017)... Pa. , March 20, 2017 PMD ... 2.0 personal spirometer and Wellness Management System (WMS), a ... Founded in 2010, PMD Healthcare is a Medical ... with a mission dedicated to creating innovative solutions that ... life. With that intent focus, PMD developed the first ...
(Date:3/9/2017)... , March 9, 2017 4Dx has publicly ... Lung Imaging Workshop at the University of Pennsylvania. Founder ... to deliver the latest data to world leaders in ... brings together leaders at the forefront of the industry ... imaging. "The quality of the imaging ...
(Date:3/7/2017)... March 7, 2017   HireVue , the leading ... companies identify the best talent, faster, today announced the ... Officer (CSO) and Diana Kucer as Chief ... seasoned executive team poised to drive continued growth in ... a year of record bookings in 2017. ...
Breaking Biology News(10 mins):
(Date:3/23/2017)... ... March 23, 2017 , ... Lajollacooks4u is proud to announce it has become the ... corporate cooking challenges for companies around the world, such as Illumina, HP and Qualcomm, and ... reason for its increasing popularity is due to its new team building format, a way ...
(Date:3/23/2017)... 2017  SeraCare Life Sciences, Inc., a manufacturer ... diagnostics manufacturers and clinical laboratories, is announcing ... Inherited Cancer reference material for ... sequencing (NGS). The Seraseq™ Inherited Cancer DNA Mix ... industry experts to validate the ability of ...
(Date:3/23/2017)... CT (PRWEB) , ... March 23, 2017 , ... ... LLC, was recently selected by the Connecticut Technology Council (CTC) as a 2017 ... at CTC’s thirteenth annual Women of Innovation Awards Dinner. , The dinner recognizes ...
(Date:3/23/2017)... 2017 According to a report by Transparency ... fragmented due to the presence of a large pool of participants; ... Fisher , and Sigma-Aldrich, compete with each other in this market. ... more than 76% of this market in 2016.  ... As of now, a large number ...
Breaking Biology Technology: