Navigation Links
New tool for RNA silencing
Date:4/1/2010

Anti-sense reagents have been developed for C. Elegans micro RNA. Researchers writing in BioMed Central's open access journal Silence have created the first class of reagents to potently and selectively inhibit miRNAs in this widely used model organism.

Wen-hong Li, from the University of Texas Southwestern Medical Center, USA, worked with a team of researchers including Dr. Genhua Zheng and Dr. Victor Ambros (University of Massachusetts Medical School) to develop this latest addition to the genetics toolkit. He said, "Caenorhabditis elegans has long been used as a model organism for studying the regulation and function of small non-coding RNA molecules, and yet no antisense reagents have been available to reliably inhibit miRNAs in worms. Our fluorescently labeled reagents were synthesized by conjugating dextran with 2'-O-methyl oligoribonucleotide, and can be conveniently introduced into the germline of adult hermaphrodites and are transmitted to their progeny".

Li's team found that their new reagents efficiently and specifically inhibited targeted miRNA in different tissues, including the hypodermis, the vulva and the nervous system. They can be used combinatorially to inhibit more than one miRNA in the same animal. They conclude, "Combined with numerous mutants or reporter stains available, these reagents should provide a convenient approach to examine genetic interactions that involve miRNA, and may facilitate studying functions of miRNAs, especially ones whose deletion strains are difficult to generate. Further, the remarkable efficacy of these antisense reagents seen in worms also suggested C. elegans as a powerful, convenient, and economical biological system to facilitate developing new chemistry and novel probes for studying miRNA and other small non-coding RNAs".


'/>"/>

Contact: Graeme Baldwin
graeme.baldwin@biomedcentral.com
44-020-319-22165
BioMed Central
Source:Eurekalert

Page: 1

Related biology news :

1. Class of antibiotics can enhance gene-silencing tool
2. Cold Spring Harbor Protocols highlights gene silencing, cancer cell biology methods
3. New gene-silencing pathway found in plants
4. Researchers solve piece of large-scale gene silencing mystery
5. In lung cancer, silencing one crucial gene disrupts normal functioning of genome
6. A new gene silencing platform -- silence is golden
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:9/22/2014)... Ill. Antarctic fishes that manufacture their own ... Ocean also suffer an unfortunate side effect, researchers ... their bodies resist melting even when temperatures warm. ... Proceedings of the National Academy of Sciences ... an undesirable consequence of the evolution of antifreeze ...
(Date:9/22/2014)... burn in Khabarovsk Krai, a territory occupying the coastline of ... plumes of smoke mark active fires. The smoke, which appears ... Sea of Okhotsk. Taiga and tundra are found ... central depression, and deciduous forests are the natural vegetation in ... in the summer, the 2014 wildfire season appears to be ...
(Date:9/22/2014)... may be at greater risk for stress-related diseases ... according to a new study by Brandeis University. ... trigger biological responses similar to the effects of ... is an important part of our body,s healing ... life-threatening diseases. , In a recently published ...
Breaking Biology News(10 mins):Study: Antifreeze proteins in Antarctic fishes prevent freezingand melting 2Study: Antifreeze proteins in Antarctic fishes prevent freezingand melting 3Obesity and stress pack a double hit for health 2
... in preserving and promoting a healthy cardiovascular system than ... researchers at the Medical College of Wisconsin in Milwaukee. ... the scientific journal Hypertension, was led by David D. ... medicine and physiology, and senior associate dean of research ...
... the red jungle fowl, but new research from Uppsala University ... more complicated than that. The researchers mapped ... found to their surprise that this genetic heredity derives from ... study is being published today in the Web edition of ...
... Option for Voluntary Use of Biometrics, WASHINGTON, ... Legal Workforce today applauded the introduction of,legislation viewed ... system and to helping prevent unauthorized,employment, a key ... The New Employee Verification Act (NEVA) introduced today ...
Cached Biology News:New study shows low-fat diets more likely to reduce risk of heart disease than low-carb diets 2Darwin was wrong about the wild origin of the chicken 2HR Initiative Endorses Bill to Create an Effective, Mandatory Federal Employment Verification System 2HR Initiative Endorses Bill to Create an Effective, Mandatory Federal Employment Verification System 3
(Date:9/22/2014)... 2014 Follow us on ... biopolymers available in abundance in nature. These natural ... such as shells of crustaceans. The significant commercial ... to the high percentage of nitrogen present compared ... various industries owing to its exclusive properties such ...
(Date:9/22/2014)... Mass. and TORONTO ... Corporation ( www.generex.com ) (OTCBB:GNBT) today announced that ... Health Network (UHN) pursuant to which UHN,s Centre ... the Company,s buccal insulin refinement project.  The goal ... bioavailability of Generex Oral-lyn™, the Company,s proprietary buccal ...
(Date:9/22/2014)... The US markets on Friday, ... the Dow Jones Industrial Average finished at 17,279.74, up ... 0.30%. The S&P 500 finished the session 0.05% lower ... ten sectors finished on a positive note. The S&P ... 751.48, up 0.10%, with the index gaining 6.95% in ...
(Date:9/21/2014)... in Switzerland have succeeded in observing the "forbidden" ... first time. These extremely weak spectra offer perspectives ... may also contribute to the development of molecular ... in the scientific journal Nature Physics . ... matter and light, is probably the most important ...
Breaking Biology Technology:Emergence of New Application Areas to Drive the Global Chitin and Chitosan Market, According to New Report by Global Industry Analysts, Inc. 2Emergence of New Application Areas to Drive the Global Chitin and Chitosan Market, According to New Report by Global Industry Analysts, Inc. 3Generex Collaborates with University Health Network for Buccal Insulin Project 2Generex Collaborates with University Health Network for Buccal Insulin Project 3Generex Collaborates with University Health Network for Buccal Insulin Project 4Stock Updates on Biotech Industry - Array BioPharma, Enzon Pharma, Alkermes, Amarin, and Synergy Pharma 2Stock Updates on Biotech Industry - Array BioPharma, Enzon Pharma, Alkermes, Amarin, and Synergy Pharma 3Stock Updates on Biotech Industry - Array BioPharma, Enzon Pharma, Alkermes, Amarin, and Synergy Pharma 4Stock Updates on Biotech Industry - Array BioPharma, Enzon Pharma, Alkermes, Amarin, and Synergy Pharma 5Uncovering the forbidden side of molecules 2
... MATK ) today announced its financial results ... 2008. Revenues for the fourth,quarter were $90.4 million, up ... 2007. Net income was $10.5 million, or $0.31 per ... with $18.3 million, or $0.55 per,diluted share, in last ...
... Myngle,( http://www.myngle.com ), the global language platform, ... at the Europe Unlimited,s European Venture Summit,(EVS) ( ... EVS is a showcase for the most ... energy entrepreneurs from all over Europe to meet,and ...
... Health-focused social networks,blogs, wikis and community ... but can be their biggest opportunities. A ... discuss increasing,importance of reputation monitoring and management ... User Generated Content and Enhancing its Impact,on ...
Cached Biology Technology:Martek Announces Fourth Quarter and FY 2008 Financial Results 2Martek Announces Fourth Quarter and FY 2008 Financial Results 3Martek Announces Fourth Quarter and FY 2008 Financial Results 4Martek Announces Fourth Quarter and FY 2008 Financial Results 5Martek Announces Fourth Quarter and FY 2008 Financial Results 6Martek Announces Fourth Quarter and FY 2008 Financial Results 7Martek Announces Fourth Quarter and FY 2008 Financial Results 8Martek Announces Fourth Quarter and FY 2008 Financial Results 9Martek Announces Fourth Quarter and FY 2008 Financial Results 10Martek Announces Fourth Quarter and FY 2008 Financial Results 11Myngle Best ICT Company at the European Ventures Summit 2Exclusive Conference on Managing Your Online Brand: Shaping the Noise from the Blogosphere 2
Human M-CSF...
Human IL-19 MAb (Clone 152112)...
Mouse Activin RIB/ALK-4 MAb (Clone 207304)...
... (1995) This book contains up ... current issues relating to the aetiology and ... have been made recently which greatly improve ... molecular neuropathology through clinical and diagnostic aspects, ...
Biology Products: