Navigation Links
New tool developed for DNA research
Date:4/6/2010

Luminescent markers are an indispensable tool for researchers working with DNA. But the markers are troublesome. Some tend to destroy the function and structure of DNA when inserted. Others emit so little light, that they can barely be detected in the hereditary material. So researchers have been asking for alternative markers.

Now a PhD student at the University of Copenhagen has developed a tool in collaboration with researchers at Chalmers Technical University, which might save both problems: A tool that you might call a molecular gauge.

PhD student Soren Preus has investigated the properties of the two luminescent so called DNA base analogues tCO and tCnitro trying to determine whether they could measure the structure of DNA without disrupting it. His scrutiny has shown that the function of DNA is unimpeded by the insertion of the molecular gauge. And even better: One base analogue is very efficient at emitting light, and the other very good at receiving it. And because you can provoke transport of light-energy between the two luminescent markers they are usable for a measuring technique known as FRET or Fluorescence Resonance Energy Transfer.

In brief FRET measurements are performed by forcing two luminescent markers to transfer light-energy from one to the other, and then measuring the efficiency of the transfer.

The two different markers are placed in the DNA-helix. When they are subjected to a lightpulse one marker (tCO) emits part of the energy to the other (tCnitro). This energy transfer can be measured. And by calculating backwards it is possible obtain very exact information about the distance and angle that the two have relative to one another.

Knowing distance and angle of the markers allows for calculations of distance and angle of all the natural base pairs in the DNA structure. And with that the researcher can put together a picture showing every twist and turn of the structure. Because structure and function are c
'/>"/>

Contact: Jes Andersen
jean@science.ku.dk
453-050-6582
University of Copenhagen
Source:Eurekalert

Page: 1 2

Related biology news :

1. UC-developed technology to assist research and genetic drug delivery
2. Novel antitoxin strategy developed using tagged binding agents
3. Yale-developed test can help predict and diagnose preeclampsia
4. No muss, no fuss miniaturized analysis for complex samples developed
5. Early life on Earth may have developed more quickly than thought
6. Ben-Gurion University of the Negev technology being developed for use in Jordan desalination plant
7. Silicon with afterburners: Process developed at Rice could be boon to electronics manufacturer
8. Skin-like tissue developed from human embryonic stem cells
9. Improved method developed to test carcinogen risk
10. Brain-computer interface, developed at Brown, begins new clinical trial
11. Next-generation software system for flood warning and risk analysis to be developed
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/10/2015)... April 10, 2015 Research and ... the "Security Competitive Profiles - NEC" report ... NEC will continue to supply a range ... market, with a company focus on the development of ... Winning opportunities in the Asia-Pacific ...
(Date:4/2/2015)... April 2, 2015 At its 2015 ACMG ... City , the American College of Medical Genetics ... directors to its Board.  Members of the ACMG Board ... for forming and advancing its policies and programs. ACMG ... "It,s an eventful time in medical genetics ...
(Date:3/31/2015)... YORK , March 31, 2015  Elephant Talk ... "Company"), a global provider of Software Defined Network Architecture ... today announced total revenue of approximately $20.4 million for ... a restated $19.5 million for the year ended December ... calendar 2014, the Company had completed its multi-year transition ...
Breaking Biology News(10 mins):NEC Security Competitive Profile 2015 2American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 2American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 3American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 4American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 5American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 6American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 7American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 8Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 2Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 3Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 4Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 5Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 6
... National Human Genome Research Institute has selected the Johns ... of Excellence" to study the ethical, legal and social ... will receive three years of funding to build on ... and public health, bringing the fields together in the ...
... HUNTSVILLE, TX (9/18/12) -- To help identify victims after ... or acts of genocide, researchers at Sam Houston State ... and speed up the DNA identification process. ... adverse conditions, such as remote locations, intense heat, and ...
... well to keep patients from developing AIDS, but no one ... the human body, so patients continue to require lifelong treatment ... team of researchers led by Dr. David N. Levy, Associate ... York University College of Dentistry (NYUCD), have discovered a new ...
Cached Biology News:Personal genome, public health 2Personal genome, public health 3Sam Houston State studies DNA preservation in mass disasters 2New HIV-1 replication pathway discovered by NYU College of Dentistry researchers 2
(Date:4/30/2015)... April 30, 2015 Available in ... http://www.lespausa.com , Phytomer’s DOUCEUR INTEMPORELLE Restorative ... reactive skin while battling the weakening threat of ... cream is formulated with star ingredient GLYCOSEA, a ... in a complex of soothing minerals and trace ...
(Date:4/30/2015)... Sterlitech is proud to offer Synder ... PY, and PZ Polyacrylonotrile (PAN) membranes , as ... to be ideal for oil removal in wastewater ... process requirements where the presence of oils in ... on industrial wastewater discharge and quality requirements for ...
(Date:4/29/2015)... ON , April 29, 2015 /PRNewswire/ - BIOREM Inc. (TSX-V: BRM) ("Biorem" ... periods ended December 31, 2014. Biorem,s complete fiscal 2014 year-end financial statements ... FINANCIAL HIGHLIGHTS: 2014 , 2013 , 2014 ... 10,683 , 19,596 Gross profit 1,326 , 1,703 ... 1,191 , 1,493 , 3,377 , ...
(Date:4/29/2015)... Colo. , April 29, 2015 ... providers of cartilage, cellular, bone, skin and soft-tissue ... care to advance patient healing, announced the launch ... Centers for Medicare & Medicaid Services (CMS) also ... from the low-cost to the high-cost category for ...
Breaking Biology Technology:Phytomer introduces DOUCEUR INTEMPORELLE Restorative Shield Cream 2Phytomer introduces DOUCEUR INTEMPORELLE Restorative Shield Cream 3Sterlitech Offers New Ultrafiltration Membranes 2Biorem Reports Results for Fourth Quarter 2014 and Year Ended December 31, 2014 2Biorem Reports Results for Fourth Quarter 2014 and Year Ended December 31, 2014 3Biorem Reports Results for Fourth Quarter 2014 and Year Ended December 31, 2014 4AlloSource Announces New Possibilities In Wound Care 2
... steps toward putting quantum computers into real practice sending ... and transmitting the signal to a second, distant qubit ... Yale. The accomplishments are reported in sequential issues of Nature ... highlighted as the cover along with complementary work from a ...
... 3% over Fiscal 2006 to $27.5 million - ... dollars unless otherwise noted), BELLEVILLE, ON, Sept. 26 ... technology-driven Canadian,biopharmaceutical company, today announced financial results for its ... announcements of the first shipment and commercial usage of,our ...
... Sept. 26 Nastech,Pharmaceutical Company Inc. (Nasdaq: NSTK ... in New York City on Thursday, October 4, 2007,from ... Westin New York at Times,Square, on 270 West 43rd ... C. Quay, M.D., Ph.D., Chairman, President and,Chief Executive Officer ...
Cached Biology Technology:Yale scientists make 2 giant steps in advancement of quantum computing 2Yale scientists make 2 giant steps in advancement of quantum computing 3Bioniche Reports Fiscal 2007 Year-End Results 2Bioniche Reports Fiscal 2007 Year-End Results 3Bioniche Reports Fiscal 2007 Year-End Results 4Bioniche Reports Fiscal 2007 Year-End Results 5Bioniche Reports Fiscal 2007 Year-End Results 6Bioniche Reports Fiscal 2007 Year-End Results 7Bioniche Reports Fiscal 2007 Year-End Results 8Bioniche Reports Fiscal 2007 Year-End Results 9Bioniche Reports Fiscal 2007 Year-End Results 10Nastech to Host 2007 Analyst and Institutional Investor Day 2Nastech to Host 2007 Analyst and Institutional Investor Day 3
... LM is a high quality microarray scanner based ... background rejection and spatial resolution. The DNAscope ... low cost microarray scanner that is upgradeable to ... Main Features: Includes control module (computer), ...
... Convenient, easy-to-use kits for arraying ... detection using common lab equipment. DiscoverLight ... analysis of your protein, cell lysate ... format. The kits include specially designed ...
... Stabilizer is an aqueous solution that ... other non-toxic stabilizing chemicals in a ... 6.5. This product contains ... 0.02% bromonitrodioxane as a preservative. ...
... When nonspecific binding is a problem ... variety of immobilized NeutrAvidin products as ... NeutrAvidin is a modified avidin derivative ... provide a biotin-binding protein with exceptionally ...
Biology Products: