Navigation Links
New technology illuminates protein interactions in living cells
Date:11/9/2007

New Haven, Conn. While fluorescence has long been used to tag biological molecules, a new technology developed at Yale allows researchers to use tiny fluorescent probes to rapidly detect and identify protein interactions within living cells while avoiding the biological disruption of existing methods, according to a report in Nature Chemical Biology.

Proteins are commonly tagged using variants of the green fluorescent protein (GFP), but these proteins are very large and are often toxic to live cells. They also tend to aggregate, making them difficult to work with and monitor. This new methodology uses the fluorescence emitted by a small molecule, rather than a large protein. It gives researchers a less disruptive way to capture images of the intricate contacts between folded regions of an individual protein or the partnerships between proteins in a live cell.

Our approach bypasses many of the problems associated with fluorescent proteins, so that we can image protein interactions in living cells, said senior author Alanna Schepartz, the Milton Harris Professor of Chemistry, and Howard Hughes Medical Institute Professor at Yale. Using these molecules we can differentiate alternative or misfolded proteins from those that are folded correctly and also detect protein partnerships in live cells.

Each protein is a three-dimensional structure created by folding its linear chain of amino acids. Usually only one shape works for each protein. The particular shape a protein takes depends on its amino acids and on other processes within the cell.

Schepartz and her team devised their new tagging system using small molecules, called profluorescent biarsenal dyes. These molecules easily enter cells and become fluorescent when they bind to a specific amino acid tag sequence within a protein. While these compounds have been used for about a decade to bind single proteins, this is the first time they have been used to identify interactions between proteins.

The researchers strategy was to split the amino acid tag for the dye into two pieces, locating each piece of the tag far apart in the chain of a protein they genetically engineered and expressed in the cells. Then they monitored cells exposed to the dye. Where the protein folded correctly, the two parts of the tag came together and the fluorescent compound bound and lit up. There was no signal unless the protein folded normally.

This method of detection can provide important insights into how proteins choose their partners within the cell choices that may be very different from those made in a test tube, said Schepartz. She emphasizes that this technology does not monitor the process of protein folding but, rather sees the protein conformations that exist at a given time.

In theory, our technique could be used to target and selectively inactivate specific protein complexes in the cell, as therapy, or to visualize conformations at very high resolution for diagnostic purposes, said Schepartz. She speculates that the technology could be applied to detection strategies that identify protein misfolding in neurodegenerative diseases like Alzheimers or Parkinsons.


'/>"/>

Contact: Janet Rettig Emanuel
janet.emanuel@yale.edu
203-432-2157
Yale University  
Source:Eurekalert

Related biology news :

1. Yale scientists use nanotechnology to fight E. coli
2. Voice Biometrics Gains Traction as Most Accurate and Convenient Technology to Secure Customer Privacy
3. M2SYS Partners with SecuGen Corporation to Support Market Leading Hamster Plus Fingerprint Reader with Auto-On Technology
4. Silicon Valley Technology Leaders LaserCard Corporation and Tesla Motors Sign LaserPass Secure Access Deal
5. Nanotechnology: Whats that?
6. UCs NIH grant brings technology from outer space to playgrounds
7. BIO-key(R) International to Showcase Deployed Biometric Security Applications at 2007 Biometric Technology Expo
8. Cogent Systems and Northrop Grumman Reach Agreement to Settle Automated Fingerprint Identification Technology Suit and Create Strategic Alliance
9. UCLA/VA partners with ASU to advance biosensor technology for urinary tract infections
10. Herr receives Heinz Award for Technology, the Economy and Employment
11. Singapore National Science and Technology Awards
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
New technology illuminates protein interactions in living cells
(Date:2/1/2016)... , February 1, 2016 ... advancements to drive global touchfree intuitive gesture control market ... --> Rising sales of consumer electronics coupled with ... control market size through 2020 ... electronics coupled with new technological advancements to drive global ...
(Date:2/1/2016)... Fla. , Feb. 1, 2016  Wocket® smart wallet ( www.wocketwallet.com ... and television personality, Joey Fatone . Las Vegas ... greet fans. --> Las Vegas , where ... --> The new video ad was filmed at the Consumer ... appeared at the Wocket booth to meet and greet fans. ...
(Date:1/27/2016)... Jan. 27, 2016  Rite Track, Inc. a leading ... West Chester, Ohio announced today the ... staff, based in Austin, Texas , ... to provide modifications, installations and technical support offerings for ... of PLUS, commented, "PLUS has provided world class service ...
Breaking Biology News(10 mins):
(Date:2/4/2016)... -- ContraVir Pharmaceuticals, Inc. (NASDAQ: CTRV ), a ... targeted antiviral therapies, announced today that it will present ... held February 8-9, 2016, at the Waldorf Astoria New ... Healthcare Conference, taking place in New York ... Sapirstein , Chief Executive Officer of ContraVir, will provide ...
(Date:2/4/2016)... California (PRWEB) , ... February 04, 2016 , ... ... Intelligence (AI) and leading supplier of Semantic Graph Database technology has been recognized ... Database Products ” by Corporate America Magazine. , “At Corporate America, it’s our ...
(Date:2/3/2016)...   ViaCyte, Inc ., a leading, privately-held ... cell-derived islet replacement therapy for the treatment of ... that ViaCyte and Janssen Biotech, Inc., one of ... have agreed to consolidate the assets of the ... ViaCyte with an exclusive license to all BetaLogics ...
(Date:2/3/2016)... -- Ascendis Pharma A/S (Nasdaq: ASND ), a ... technology to address significant unmet medical needs, today announced ... Partners Global Healthcare Conference Location: , Waldorf Astoria, ... Time:  , 11:55am EST www.ascendispharma.com . ... audio webcast of this event will be posted to ...
Breaking Biology Technology: