Navigation Links
New technology allows scientists to watch cancer cells in action at unprecedented resolution
Date:2/1/2012

A photograph of a polar bear in captivity, no matter how sharp the resolution, can never reveal as much about behavior as footage of that polar bear in its natural habitat. The behavior of cells and molecules can prove even more elusive. Limitations in biomedical imaging technologies have hampered attempts to understand cellular and molecular behavior, with biologists trying to envision dynamic processes through static snapshots.

Deborah Kelly, an assistant professor in the Virginia Tech Carilion Research Institute, has now developed a novel technology platform to peer closely into the world of cells and molecules within a native, liquid environment.

Kelly and colleagues have developed a way to isolate biological specimens in a flowing, liquid environment while enclosing those specimens in the high-vacuum system of a transmission electron microscope (TEM). The TEM liquid-flow holder, developed by Protochips Inc. of Raleigh, N.C., accommodates biological samples between two semiconductor microchips that are tightly sealed together. These chips form a microfluidic device smaller than a Tic Tac. This device, positioned at the tip of an EM specimen holder, permits liquid flow in and out of the holder. When these chips are coated with a special affinity biofilm that Kelly developed, they have the ability to capture cells and molecules rapidly and with high specificity. This system allows researchers to watch -- at unprecedented resolution -- biological processes as they occur, such as the interaction of a molecule with a receptor on a cell that triggers normal development or cancer.

"With this new technology, we can capture and view the native architecture of cells and their surface protein receptors while learning about their dynamic interactions, such as what happens when cells interact with pathogens or drugs," said Kelly. "We can now isolate cancer cells, for example, and view the early events of chemotherapy in action."

Kelly had previously worked with colleagues at Harvard Medical School to develop a way to capture protein machinery in a frozen environment. "But life moves," said Kelly. "It's better if biological processes don't have to be paused or frozen in order to be studied, but can be viewed in dynamic and life-sustaining liquid environments."

Kelly's affinity capture device, in combination with high-resolution TEM, helps bridge the gap between cellular and molecular imaging, allowing researchers to achieve spatial resolution as high as two nanometers. "This device allows us to see new features on the surface of live cancer cells, providing new targets for drug therapy," Kelly said. "With this resolution, scientists may even be able to visualize disease processes as they unfold."

The research appears in the February issue of RSC Advances, an international journal of the Royal Society of Chemistry of London, in the article "The development of affinity capture devices -- a nanoscale purification platform for biological in situ transmission electron microscopy," by Katherine Degen, a biomedical engineering student at the University of Virginia; Madeline Dukes, an applications scientist at Protochips; Justin Tanner, a postdoctoral associate at the Virginia Tech Carilion Research Institute; and Kelly, the corresponding author. The link to the article is http://pubs.rsc.org/en/content/articlelanding/2012/ra/c2ra01163h


'/>"/>

Contact: Paula Byron
paulabyron@vt.edu
540-526-2027
Virginia Tech
Source:Eurekalert  

Related biology news :

1. MSU technology spin-out company to market portable biohazard detection
2. PNNL recognized for technology transfer
3. Nanotechnology in Medical Applications: The Global Market
4. T-rays technology could help develop Star Trek-style hand-held medical scanners
5. 2012 Biotechnology and Pharmaceuticals: Forthcoming Conferences and Exhibitions
6. BGI enters agreement with National Center for Soybean Biotechnology to re-sequence 1,008 soybean lines
7. Neurotechnology Announces VeriFinger, VeriLook and MegaMatcher Biometric Technologies for the Android Platform
8. White House official heads keynote speakers at Carbon Management Technology Conference
9. Naval technology could be a lifesaver
10. Exploiting Trichoderma: From food security to biotechnology
11. UCF nanotechnology may speed up drug testing
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
New technology allows scientists to watch cancer cells in action at unprecedented resolution
(Date:8/23/2017)... Aug. 23, 2017  The general public,s help is being enlisted in ... that live in and on the human body –and are believed to ... The Microbiome Immunity Project ... microbiome, starting with the gut. The project's goal is to help advance ... IBM ...
(Date:6/30/2017)... ARLINGTON, Va. , June 30, 2017 /PRNewswire-USNewswire/ ... a leading developer and supplier of face and ... the ATA Featured Product provider program. ... created an innovative way to monitor a driver,s ... benefit greatly from being able to detect fatigue ...
(Date:5/23/2017)... , May 23, 2017  Hunova, the first robotic gym for ... has been officially launched in Genoa, Italy . The ... and the USA . The technology was ... the market by the IIT spin-off Movendo Technology thanks to a 10 ... Multimedia News Release, please click: ...
Breaking Biology News(10 mins):
(Date:10/12/2017)... ... October 12, 2017 , ... AMRI, ... and biotechnology industries to improve patient outcomes and quality of life, will now ... testing are being attributed to new regulatory requirements for all new drug products, ...
(Date:10/11/2017)... Alto, CA, USA (PRWEB) , ... October 11, 2017 , ... ... set to take place on 7th and 8th June 2018 in San Francisco, CA. ... policy influencers as well as several distinguished CEOs, board directors and government officials from ...
(Date:10/11/2017)... (PRWEB) , ... October 11, 2017 , ... ... compared the implantation and pregnancy rates in frozen and fresh in vitro ... of progesterone and maternal age to IVF success. , After comparing the results ...
(Date:10/10/2017)... ... October 10, 2017 , ... Dr. Bob Harman, founder and CEO of ... Club. The event entitled “Stem Cells and Their Regenerative Powers,” was ... Dr. Harman, DVM, MPVM was joined by two human doctors: Peter B. Hanson, M.D., ...
Breaking Biology Technology: