Navigation Links
New technique yields more detailed picture of chromatin structure
Date:4/16/2008

exposed living animal cells to the labeled antibodies. This allowed the antibodies to penetrate more deeply into the chromatin structure, and boosted the number of gold particles adhering to regions of interest. The signal was enhanced by adding a silver solution that precipitated (solidified) upon contact with the gold.

We are interested in chromatin structure, so our targets are mostly chromatin-bound proteins, Kireev said.

The researchers had inserted several copies of a bacterial DNA, called the Lac operator, into the chromosomes. A bacterial protein, the Lac repressor, recognizes and binds to the Lac operator in living cells.

The researchers combined a Lac repressor protein with another protein that fluoresces green under blue light. This engineered protein adhered to the chromosomes in regions containing the Lac operator sequences. Under blue light, these regions fluoresced. A gold-tagged antibody targeted against green fluorescent protein (GFP) was then microinjected into the nucleus of a living cell, which added a metallic signal that could be boosted with silver.

All this combined gives us a much better signal, a much stronger signal, with the very best structural preservation, Kireev said.

The fluorescing protein helped the researchers find the regions of interest in the cells. These areas were then immunogold labeled and targeted for electron microscopy.

In the resulting micrographs the researchers saw enhanced staining of the chromosomes.

We can now apply this same live-cell labeling method to study at high resolution many different GFP-tagged proteins in the cell cytoplasm or nucleus, said Andrew Belmont, a professor of cell and developmental biology and senior author of the paper.

In trying to understand chromosomes, people have largely been limited to low resolution visualization of specific chromosomal proteins using light microscopy, Belmost said. This meant everyone has had to
'/>"/>

Contact: Diana Yates
diya@uiuc.edu
217-333-5802
University of Illinois at Urbana-Champaign
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. New technique can be breakthrough for early cancer diagnosis
2. University of Leicester scientists discover technique to help friendly bacteria
3. New technique reveals subtle force-induced changes in biomolecules conformation
4. New magnetic separation technique might detect multiple pathogens at once
5. New technique captures chemical reactions in a single living cell at unprecedented resolution
6. Software Techniques Inc. Selects M2SYS Fingerprint Software to Meet Continuous Growth and Demand for Desktop Biometric Time & Attendance Solutions
7. New X-ray technique targets terrorists and tumors
8. Technique controls nanoparticle size, makes large numbers
9. New technique could dramatically lower costs of DNA sequencing
10. New techniques create butanol
11. New radar satellite technique sheds light on ocean current dynamics
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
New technique yields more detailed picture of chromatin structure
(Date:4/2/2015)... 2, 2015 According to preliminary ... to somewhat higher than 125 MSEK. This exceeds the ... 2014 that revenues for Q1 2015 would be in ... for Q4 2014. The operating result for the first ... complete interim report will as previously communicated be published ...
(Date:4/1/2015)... , April 1, 2015   Medisafe ™, ... million users across iOS and Android ... begun integrating biofeedback into the platform to allow patients ... outcomes. For the first time, patients will be able ... a dose impacts important biometrics, such as glucose levels ...
(Date:3/30/2015)... 26, 2015 Research and Markets ( ... "Global Gesture Recognition Market in Automotive Sector 2014-2018" ... the Global Gesture Recognition market in Automotive Sector to ... 2013-2018. Gesture recognition is the ability of ... of an individual. Gesture recognition technology can be 2D-based ...
Breaking Biology News(10 mins):Fingerprint Cards Revises Revenues for Q1 2015 2Medisafe Announces Launch of Biometric Integration, Enabling Patients to Stay Adherent to Their Meds and See Tangible Health Results 2Global Gesture Recognition Market in Automotive Sector Report 2015 - Growing Number of Partnerships 2
... "red light green light," winners are able to stop, ... comrades. New research reveals that a similar race goes ... big winner. , "The research provides new insights into ... impulsivity of people with attention deficit and hyperactivity disorder," ...
... on how erectile dysfunction (ED) interacts with diabetes. The ... the two disorders, and may lead to improved efficacy ... Nitric Oxide Triggers Erectile Dysfunction in Diabetes," was conducted ... Patel, Departments of Cellular and Integrative Physiology; and Keshore ...
... University of Missouri-Columbia, recently found fossil evidence in seven ... that showed people were eating domesticated chili peppers as ... one of the oldest domesticated food sources in the ... 15 edition of the journal Science. , "Before our ...
Cached Biology News:Racing neurons control whether we stop or go 2Racing neurons control whether we stop or go 3Erectile dysfunction in diabetes is due to selective defect in the brain 2Erectile dysfunction in diabetes is due to selective defect in the brain 3Researchers find 6,000-year-old fossil evidence 2
(Date:4/16/2015)... , April 16, 2015 Avelas Biosciences, an ... improving cancer surgeries, announced today that it has initiated ... illuminator, AVB-620, in women with primary, non-recurrent breast cancer ... also welcomed the addition of Steven Chen , ... The open label, dose escalation study will enroll up ...
(Date:4/16/2015)... Switzerland , April 16, 2015 ... of Debiopharm Group ™ , a Swiss-based global ... unmet medical needs as well as companion ... the investigational compounds Debio 1143 (SMAC mimetic) and ... at the 2015 Annual meeting of the ...
(Date:4/16/2015)... (PRWEB) April 16, 2015 Fairleigh ... Joseph V. Gulfo to become the Executive Director ... Simultaneously, FDU announced the kick-off of the Initiative ... spearheaded by Dr. Gulfo, under the Rothman Institute ... of Business) and in collaboration with FDU’s School ...
(Date:4/16/2015)... Dana-Farber Cancer Institute and its ... Bioscience, Inc. today announced the successful completion of ... clinic ready, Next Generation Sequencing (NGS) assay for ... the first to demonstrate the successful sequencing of ... blinded plasma samples and highlights the ability to ...
Breaking Biology Technology:Avelas Biosciences Initiates Phase 1b Trial of AVB-620 in Breast Cancer Patients 2Avelas Biosciences Initiates Phase 1b Trial of AVB-620 in Breast Cancer Patients 3Debiopharm International SA Announces Results of 2 Key Oncology Programs at 2015 AACR Conference 2Debiopharm International SA Announces Results of 2 Key Oncology Programs at 2015 AACR Conference 3Dr. Joseph V. Gulfo Named Executive Director of Fairleigh Dickinson University’s Rothman Institute to Spearhead New Initiative for Patient-Centered Innovation (iPCI) 2Dr. Joseph V. Gulfo Named Executive Director of Fairleigh Dickinson University’s Rothman Institute to Spearhead New Initiative for Patient-Centered Innovation (iPCI) 3Resolution Bioscience announces successful collaboration on a novel, non-invasive, targeted NGS test for lung cancer 2Resolution Bioscience announces successful collaboration on a novel, non-invasive, targeted NGS test for lung cancer 3
... Double 2009 Grant Awards to Nearly $4 Million , ... Lustgarten Foundation, the nation,s largest private supporter of pancreatic cancer ... $1.6 million awarded to six leading research centers for early ... deadliest cancers. , , Lustgarten is expected to ...
... , VIENNA, Austria, June 23 ... 2009 Venture Capital & Private Equity Award,competition, hosted by the ... association between the,German venture fund and the Austrian biotechnology company ... GSK Biologicals. , Last Tuesday MIG ...
... Calif., June 22 ForteBio(R), Inc., a leading ... of biotherapeutic and pharmaceutical products, announced today that ... RED384 for protein, peptide, small molecule and fragment ... antibody assays. The new instruments will debut this ...
Cached Biology Technology:The Lustgarten Foundation Awards Six Research Centers With Pancreatic Cancer Research Funding 2AFFiRiS AG: 1st Place in Venture Capital & Private Equity Awards for MIG Fonds and AFFiRiS 2AFFiRiS AG: 1st Place in Venture Capital & Private Equity Awards for MIG Fonds and AFFiRiS 3ForteBio Launches Two Revolutionary High-Throughput, Label-Free Instruments: The Octet(R) RED384 and the Octet QK384 2ForteBio Launches Two Revolutionary High-Throughput, Label-Free Instruments: The Octet(R) RED384 and the Octet QK384 3