Navigation Links
New technique yields more detailed picture of chromatin structure
Date:4/16/2008

exposed living animal cells to the labeled antibodies. This allowed the antibodies to penetrate more deeply into the chromatin structure, and boosted the number of gold particles adhering to regions of interest. The signal was enhanced by adding a silver solution that precipitated (solidified) upon contact with the gold.

We are interested in chromatin structure, so our targets are mostly chromatin-bound proteins, Kireev said.

The researchers had inserted several copies of a bacterial DNA, called the Lac operator, into the chromosomes. A bacterial protein, the Lac repressor, recognizes and binds to the Lac operator in living cells.

The researchers combined a Lac repressor protein with another protein that fluoresces green under blue light. This engineered protein adhered to the chromosomes in regions containing the Lac operator sequences. Under blue light, these regions fluoresced. A gold-tagged antibody targeted against green fluorescent protein (GFP) was then microinjected into the nucleus of a living cell, which added a metallic signal that could be boosted with silver.

All this combined gives us a much better signal, a much stronger signal, with the very best structural preservation, Kireev said.

The fluorescing protein helped the researchers find the regions of interest in the cells. These areas were then immunogold labeled and targeted for electron microscopy.

In the resulting micrographs the researchers saw enhanced staining of the chromosomes.

We can now apply this same live-cell labeling method to study at high resolution many different GFP-tagged proteins in the cell cytoplasm or nucleus, said Andrew Belmont, a professor of cell and developmental biology and senior author of the paper.

In trying to understand chromosomes, people have largely been limited to low resolution visualization of specific chromosomal proteins using light microscopy, Belmost said. This meant everyone has had to
'/>"/>

Contact: Diana Yates
diya@uiuc.edu
217-333-5802
University of Illinois at Urbana-Champaign
Source:Eurekalert  

0

GOOD

Page: 1 2 3

Related biology news :

1. New technique can be breakthrough for early cancer diagnosis
2. University of Leicester scientists discover technique to help friendly bacteria
3. New technique reveals subtle force-induced changes in biomolecules conformation
4. New magnetic separation technique might detect multiple pathogens at once
5. New technique captures chemical reactions in a single living cell at unprecedented resolution
6. Software Techniques Inc. Selects M2SYS Fingerprint Software to Meet Continuous Growth and Demand for Desktop Biometric Time & Attendance Solutions
7. New X-ray technique targets terrorists and tumors
8. Technique controls nanoparticle size, makes large numbers
9. New technique could dramatically lower costs of DNA sequencing
10. New techniques create butanol
11. New radar satellite technique sheds light on ocean current dynamics
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
New technique yields more detailed picture of chromatin structure
(Date:5/15/2013)... behavioral effects on diverse organisms often play large ... a theory proposed in the June issue of ... Ryan P. Ferrer of Seattle Pacific University and ... at Los Angeles, liken such molecules to keystone ... but exert a controlling influence, through predation or ...
(Date:5/15/2013)... of reducing greenhouse gas (GHG) emissions to 17 per ... federal and provincial governments, led by the Prime Minister ... of the total GHG reduction will be provided by ... School of the Environment. Their report is being ... parties and other participants in the climate policy dialogue. ...
(Date:5/15/2013)... strategic plan, " Forging the Future A Ten-Year ... Genome Institute (DOE JGI) has positioned itself to provide ... so that they can address pressing energy and environmental ... in this process is the launch of the ... of the ETOP is to develop and support selected ...
Breaking Biology News(10 mins):Natural 'keystone molecules' punch over their weight in ecosystems 2Canada must addess real climate-change challenge 2Canada must addess real climate-change challenge 3The DOE Joint Genome Institute expands capabilities via new partnerships 2The DOE Joint Genome Institute expands capabilities via new partnerships 3
... virus has a problem: it is a giant among viruses ... cell and reproduce. Professor Ari Helenius and Postdoc Jason Mercer ... this strategy is. In the process, they stumbled upon new ... In order to infiltrate a cell, the vaccinia virus ...
... a broad range of genetic tests. Paternity tests ... future baldness. With ongoing genetic discoveries and ... ever before and along with greater availability has ... How can consumers make informed decisions about genetic ...
... issues is the development of techniques that increase the ... 24, 2008 in the journal Vaccine, a Massachusettss biotechnology ... for anti-viral vaccination. This technology consists of two major ... two forms. One is easily processed within the organisms ...
Cached Biology News:A step forward in virology 2A step forward in virology 3American College of Medical Genetics makes genetic testing recommendations in new policy statement 2New technology for boosting vaccine efficiency 2New technology for boosting vaccine efficiency 3
(Date:5/16/2013)... A new selection of laboratory ... is designed to meet the dynamic needs of ... new Cole-Parmer® MS-3400 Centrifuges are clinical ... They ensure a powerful and complete separation of ... standard four-place swinging buckets hold 50-mL tubes. With ...
(Date:5/16/2013)... 2013 Kitware , a ... announces new Phase II SBIR funding from the ... of an open-source, high performance computing (HPC) simulation ... enables researchers to select only the toolkits and ... computation, or analysis tasks. , Manufacturers increasingly ...
(Date:5/16/2013)... -- The registration deadline has been extended until May 22 ... Conference, the premier conference for bioscience financial officers. The ... San Diego Bayfront. "We are putting ... to make sure as many people as possible have ... , Chief Financial Officer of Mast Therapeutics, Inc. ...
(Date:5/16/2013)... 2013 As the weather warms, women everywhere ... Spring looks. While most make a statement with fashion, many ... find the warmer weather mixes makeup and sweat to produce, ... onset of acne during warmer months, Adult Acne treatment ... users the best ways to deter breakouts. , Recently, ...
Breaking Biology Technology:Cole-Parmer Offers Enhanced Selection of Centrifuges 2Kitware Develops a Customizable Simulation Framework to Provide HPC for Small to Mid-Sized Manufacturers 2Registration Extended Until May 22 for Association of Bioscience Financial Officers (ABFO) 2013 National Conference -- San Diego 2Registration Extended Until May 22 for Association of Bioscience Financial Officers (ABFO) 2013 National Conference -- San Diego 3Registration Extended Until May 22 for Association of Bioscience Financial Officers (ABFO) 2013 National Conference -- San Diego 4Adult Acne Treatment, Probiotic Action Shares New Tips for Women on How to Fight Acne While Wearing Makeup 2
... Calif., May 21 AutoGenomics, a leader in developing ... team comprised of the Canada Research Chair in Medical ... new viruses and antiviral resistance, Professor Guy Boivin has ... for the presence of the new Flu A - ...
... giveaways show state,s growing germ-fighting focus ATLANTA, ... contingent to this week,s Biotechnology Industry Organization (BIO) ... they press the flesh with thousands of conference ... is distributing hundreds of containers of hand sanitizers ...
... (the "Company") (Nasdaq: KERX ) announced today the ... Company. It is also anticipated that Ron Bentsur will join ... the board on June 16, 2009.Michael P. Tarnok, Interim Chairman ... the Board, I want to welcome Ron Bentsur to Keryx. ...
Cached Biology Technology:AutoGenomics New RVP Assay Addresses Current Challenge to Detect Flu A - Swine H1N1 2BIO Visitors Bring Viral Preparedness to Atlanta 2BIO Visitors Bring Viral Preparedness to Atlanta 3Keryx Biopharmaceuticals Announces Appointment of Ron Bentsur as Chief Executive Officer 2Keryx Biopharmaceuticals Announces Appointment of Ron Bentsur as Chief Executive Officer 3Keryx Biopharmaceuticals Announces Appointment of Ron Bentsur as Chief Executive Officer 4
Anti-BAR Immunogen: Peptide of aa 3-21 from human Bar Available Date: 38226...
... UbcH7 interacts with the HECT ... RING domain. Evidence suggests that ... both the degradation of non-""N-end ... certain N-a -acetylated proteins as ...
Anti-Mouse D011.10 TCR, Biotin (Clone KJ1-26) (mouse IgG2a)...
Reacts with methylated DNA from plants and vertebrates...
Biology Products: