Navigation Links
New technique tracks viral infections, aids development of antiviral drugs
Date:4/11/2011

, while highly sensitive, involve the lysis [the breaking down] of cells prior to measurement and are therefore unable to provide information about cellular viability, infected cell phenotypes, percentage of infected cells or the variation in infection among a cell population. The LNA probe differs from traditional nucleotide probes by binding more tightly to its target RNA.

LNA-flow FISH presents a fast and easy way to screen for compounds with antiviral activity and could be adapted for monitoring infections in the blood for vaccine therapy and development. This method adds a necessary tool for several emerging areas in cell biology that enables the use of high throughput measurements for entire populations and improves statistical analyses.

"This method can be expanded by adding more than one kind of LNA probe to enable multiple detection of different viral and host RNA," adds Robertson. "The multiplexing enhancement can be used to better understand infectious agents, allowing this technique to be used to aid in the development of antiviral drugs for a variety of viruses."

LNA flow-FISH offers an advantage over other techniques due to its simplicity and superiority. Methods involving genetic recombination of the virus to express a fluorescent protein as a means to mark the presence of virus can utilize flow cytometry for large-batch analysis of infected cells. However, an exception to this approach is viral strains that have not acquired genetic mutations, known as wild-type viruses (such as strains of Human Immunodeficiency Virus-HIV), which would require a large initial investment of labor for engineering each virus of interest.


'/>"/>

Contact: Daniel Parry
daniel.parry@nrl.navy.mil
202-767-2541
Naval Research Laboratory
Source:Eurekalert  

Page: 1 2

Related biology news :

1. Mapping the brain: New technique poised to untangle the complexity of the brain
2. DNA stretching -- A new technique being carried out at CIC microGUNE to detect illnesses
3. New imaging technique provides rapid, high-definition chemistry
4. New high-resolution carbon mapping techniques provide more accurate results
5. Combined molecular study techniques reveal more about DNA proteins
6. New non-surgical autopsy technique set to revolutionize post-mortem practice
7. NYU nursing-dental team receives grant to assess effectiveness of A1C diabetes screening technique
8. Ben-Gurion U. researchers develop techniques to manipulate plant adaption in arid climates
9. NIST technique controls sizes of nanoparticle clusters for EHS studies
10. New technique to see neurons of the deep brain for months at a time developed at Stanford
11. Technique allows researchers to identify key maize genes for increased yield
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
New technique tracks viral infections, aids development of antiviral drugs
(Date:7/2/2015)... DUBLIN , June 25, 2015 ... the addition of the "Next Generation Biometrics Market ... to 2020" report to their offering. ... $24.4 Billion by 2020, at a CAGR of 17.9% ... is the leading application for the market. Safran ...
(Date:6/29/2015)... June 24, 2015 Research ... the "Latin America Biomedical Sensors Market - Growth, ... The Latin America Biomedical Sensors market is ... of 2.04% over the period 2014-2020 The ... to the genetic formulation of each individual. These sensors ...
(Date:6/25/2015)... 2015  TAKE Solutions Ltd., a global business technology ... States Patent and Trademark Office (USPTO) for its "Method ... Solutions, Clinical Accelerators to reduce the time taken to ... standardization without the accelerators), thus reducing time to market. ... At the heart of the patented Clinical Data Standardization ...
Breaking Biology News(10 mins):Next Generation Biometrics Market 2015 - Global Forecast to 2020 with Safran, NEC, 3M Cogent, Fujitsu & Suprema Dominating the $24 Billion Market 2Next Generation Biometrics Market 2015 - Global Forecast to 2020 with Safran, NEC, 3M Cogent, Fujitsu & Suprema Dominating the $24 Billion Market 3Latin America Biomedical Sensors Market Report 2015-2020 2TAKE Solutions Awarded Patent By USPTO 2
... marginalized groups including the mentally ill, the disabled ... fare worse than others in the aftermath of ... Now, a new study by UCLA ... greater terrorism-related fears and make more behavioral changes based ...
... used to fumigate termite-infested buildings is a strong greenhouse ... longer than previously thought, UC Irvine research has found. ... atmosphere at least 30-40 years and perhaps as long ... at as low as five years, grossly underestimating the ...
... an expedition in 1965 to trap moths on Mount Kinabalu ... the groundwork for a study of the impacts of climate ... York has repeated the survey 42 years later, and found ... metres over the intervening years to cope with changes in ...
Cached Biology News:Disabled and other vulnerable groups more susceptible to terrorism fears 2Termite insecticide a potent greenhouse gas 2The global impact of climate change on biodiversity 2
(Date:7/3/2015)... ... ... The global failure analysis market is expected to reach $7,147.0 million by 2020, ... to grow at the highest CAGR of 8.46%; its growth is expected to be ... academic institutions for root cause analysis of failure. The market based on the geographic ...
(Date:7/3/2015)... July 3, 2015 ... september 2015 på  http://www.openinnovationinscience.at om efteruddannelsesprogrammet ... finder sted i Wien ... af internationale forskere og videnskabsmænd foretaget af ... største udfordringer for sundhedsvidenskaben manglen på incitamenter ...
(Date:7/2/2015)... Scotland , July 2, 2015 ... Bio-tech procurement company Amici Procurement has chosen ... to help it move to Office 365. ... provides small to medium-sized companies in the bio-tech ... all aspects of the purchasing cycle to help them ...
(Date:7/1/2015)... and PARIS , July ... and Genective, key developer of biotech crops, today ... of new generations of insect control traits. ... to counter the realities of advancing insect resistance. ... AgBiome,s unique insect control technology with Genective,s capability ...
Breaking Biology Technology:Failure Analysis Market to Grow at 7.52% CAGR to 2020 2Failure Analysis Market to Grow at 7.52% CAGR to 2020 3Failure Analysis Market to Grow at 7.52% CAGR to 2020 4Failure Analysis Market to Grow at 7.52% CAGR to 2020 5Lancering af verdens første åbne innovationsprogram til uddannelse af forskere 2Lancering af verdens første åbne innovationsprogram til uddannelse af forskere 3Biotech Procurement Company Moves to Office 365 with iomart 2AgBiome and Genective collaborate to create novel insect-resistant crops 2
... OXFORD, England, September 10 PharmaVentures ... of the Product & Portfolio,Strategy Practice of Corporate ... , Fintan Walton, PhD, ... welcome Nikki to PharmaVentures. Our Corporate Advisory business,is ...
... , In the news release, ... PhD, as Editor for a Refocused Pediatric,Adolescent, & Maternal ... Clinical Therapeutics over PR Newswire, we are advised by ... changed from "Bridgewater, England",to "Bridgewater, New Jersey" and the ...
... SEATTLE, Sept. 10 Cell Therapeutics, Inc. (CTI) (Nasdaq and ... conference on Wednesday, September 16, 2009, at 10:30 AM Eastern. , ... the Millennium Broadway Hotel in New York. The conference will be ... webcast can be accessed at www.celltherapeutics.com . , ...
Cached Biology Technology:PharmaVentures Corporate Advisory Appoints Nikki Watkins as Practice Head, Product & Portfolio Strategy 2/C O R R E C T I O N -- Clinical Therapeutics/ 2/C O R R E C T I O N -- Clinical Therapeutics/ 3/C O R R E C T I O N -- Clinical Therapeutics/ 4/C O R R E C T I O N -- Clinical Therapeutics/ 5
... Suitable for 0.2ml thermal cycler blocks, 96-well PCR ... adhesive and heat seals), Cut-away corner , Fits ... 3100 and 3700 capillary sequencers, Clear well bottom ... using Thermo-Mats, Adhesive Films and Foils, Heat Seals ...
... One-Step Kit is the ultimate in ... It has been specifically designed to ... Reverse-iT™ One-Step Kit combines the unique ... Thermoprime Plus DNA Polymerase to provide ...
...
... A and Protein G bind specifically to ... A and Protein G conjugates are commonly ... (antibodies) and immunoglobulin subtypes from serum, hybridoma ... biological fluids. These reagents are also commonly ...
Biology Products: