Navigation Links
New technique tracks viral infections, aids development of antiviral drugs
Date:4/11/2011

WASHINGTON -- Scientists at the Naval Research Laboratory Center for Bio-Molecular Science and Engineering have developed a method to detect the presence of viruses in cells and to study their growth. Targeting a virus that has ribonucleic acid (RNA) as its genetic makeup, the new technique referred to as locked nucleic acid (LNA) flow cytometry-fluorescence in situ hybridization (flow-FISH), involves the binding of an LNA probe to viral RNA.

While individual parts of the technique have been developed previously, Drs. Kelly Robertson and Eddie Chang, in collaboration with researchers at the NRL Lab for Biosensors and Biomaterials, demonstrate for the first time that the combination of LNA probes with flow-FISH can be used to quantify viral RNA in infected cells. This also allows the scientists to monitor the changes in viral RNA accompanying antiviral drug treatment.

Once the probe is bound to the viral RNA inside mammalian cells, it is tagged with a fluorescent dye, then thousands of these tagged cells are measured rapidly by "flow cytometry" a method for counting and examining microscopic particles, such as cells and chromosomes, by suspending them in a stream of fluid and passing them by an electronic detection apparatus.

"The ability to rapidly measure thousands of cells for the presence of virus, sets this technique apart from currently used methods to monitor viral replication," said Robertson.

Traditionally, antibodies used to detect viruses must be produced and calibrated for each specific strain and are highly susceptible to viral mutations. Assays commonly used for quantifying viral loads and for drug development can be time consuming and rely on visible signs of cell damage, which is not produced in all viruses and can take long periods of time to occur.

Techniques such as quantitative reverse transcription-polymerase chain reaction (qRT-PCR), microarrays, and enzyme-linked immunosorbent assays (ELISAs)
'/>"/>

Contact: Daniel Parry
daniel.parry@nrl.navy.mil
202-767-2541
Naval Research Laboratory
Source:Eurekalert  

Page: 1 2

Related biology news :

1. Mapping the brain: New technique poised to untangle the complexity of the brain
2. DNA stretching -- A new technique being carried out at CIC microGUNE to detect illnesses
3. New imaging technique provides rapid, high-definition chemistry
4. New high-resolution carbon mapping techniques provide more accurate results
5. Combined molecular study techniques reveal more about DNA proteins
6. New non-surgical autopsy technique set to revolutionize post-mortem practice
7. NYU nursing-dental team receives grant to assess effectiveness of A1C diabetes screening technique
8. Ben-Gurion U. researchers develop techniques to manipulate plant adaption in arid climates
9. NIST technique controls sizes of nanoparticle clusters for EHS studies
10. New technique to see neurons of the deep brain for months at a time developed at Stanford
11. Technique allows researchers to identify key maize genes for increased yield
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
New technique tracks viral infections, aids development of antiviral drugs
(Date:7/28/2014)... a mechanism in part of the brain that is key ... both type 1 and type 2 diabetes. The findings are ... the National Academies of Sciences . , "We,ve discovered ... of the hypothalamus known as the ventromedial nucleus sets ... in the blood," said lead author Sabrina Diano, professor in ...
(Date:7/28/2014)... Solanum pennellii , a wild relative of the domestic ... researchers including the labs headed by Professors Neelima Sinha ... Plant Biology. The new genome information may help breeders ... July 27 in the journal Nature Genetics , ... University in Germany. The UC Davis labs carried out ...
(Date:7/28/2014)... is no cure for Alzheimer,s disease and other forms ... closer to finding treatment. , University of Washington bioengineers ... the harmful changes of the body,s normal proteins into ... Alzheimer,s, Parkinson,s, heart disease, Type 2 diabetes and Lou ... they shift from their normal state into an abnormally ...
Breaking Biology News(10 mins):Glucose 'control switch' in the brain key to both types of diabetes 2New protein structure could help treat Alzheimer's, related diseases 2New protein structure could help treat Alzheimer's, related diseases 3
... industry is being significantly challenged as production levels ... means for improving the industry,s competitiveness are being ... Manufacturing, Innovation, Trade, Resources and Energy (DMITRE) has ... to undertake a study that identifies potential routes ...
... restrictions have become unnecessarily restrictive and expensiveon Mars., ... astrobiologists Alberto Fairn of Cornell University and Dirk ... Office of Planetary Protection,s "detailed and expensive" efforts ... missions to search for life on the red ...
... Research Careers) Program has announced the travel award recipients ... in Immunology meeting in Boston, MA from July 28-August ... entry of students, postdoctorates and scientists from underrepresented groups ... to encourage the participation of young scientists at the ...
Cached Biology News:Bioeconomy as a solution for South Australia's declining forest industry 2Bioeconomy as a solution for South Australia's declining forest industry 3Researchers call for rethinking efforts to prevent interplanetary contamination 2
(Date:7/29/2014)... California (PRWEB) July 29, 2014 The ... is a next generation approach to robotics. What sets ... is patent pending technology that allows them to communicate, ... all wirelessly over the internet. , “The invention is ... giving the little Droidles a life of their own, ...
(Date:7/29/2014)... (PRWEB) July 29, 2014 White ... devices, and cloud based eClinical technologies, have been published ... Online. These follow a long line of white papers ... publications including Life Science Leader, Pharmaceutical Online, and OpenHealthNews. ... provided a paper by CTO Marc Desgrousilliers, 'The ...
(Date:7/28/2014)... , July 28, 2014 Sapphire Energy, ... today announced the appointment of biotechnology veteran James ... board of directors. Levine replaces Cynthia ,CJ, Warner, who ... will remain chairman of the company,s board of directors. ... Energy,s mission to deliver commercial scale algae-based fuels has ...
(Date:7/28/2014)... (PRWEB) July 28, 2014 Proove ... medicine, will be exhibiting data and research conducted through ... International Spine Intervention Society’s (ISIS) 22nd Annual Scientific ... at the Hyatt Regency, in Orlando, Florida. ... and clinical competence of physicians who care for patients ...
Breaking Biology Technology:Droidles, the Social Network of Robots and Open Source Platform for the Internet of Things, Launches for Crowd Funding on IndieGoGo.com 2Clinovo White Papers Published in Summer 2014 Editions of Leading Medical Device & Pharmaceutical Publications 2Clinovo White Papers Published in Summer 2014 Editions of Leading Medical Device & Pharmaceutical Publications 3Sapphire Energy Announces New CEO James Levine 2Sapphire Energy Announces New CEO James Levine 3Proove Biosciences Exhibits Industry Leading Data and Research at International Spine Intervention Society’s 22nd Annual Scientific Meeting 2
... ... Support Innovative Start-up Companies and Entrepreneurs in Communications and Communications Convergence , ... San Diego, CA (PRWEB) June ... of Cricket Communications, Inc., a leading provider of unlimited wireless services, today announce their ...
... Germany, June 17 , - MedITEX ... Medicine , - MedITEX from 28 June ... , The IT consulting company CRITEX, which ... in vitro fertilisation, MedITEX IVF,at the 25th Annual Meeting of the ...
... 16 Microfluidic Systems, a privately-held company, announced ... "Sonication to Selectively Lyse Different Cell Types". This ... extraction of male/female DNA from a mixed forensic ... of biological sample processes for analysis and identification. ...
Cached Biology Technology:San Diego Wireless Heavyweight Leap Wireless Supports New Start-up Incubator 2San Diego Wireless Heavyweight Leap Wireless Supports New Start-up Incubator 3San Diego Wireless Heavyweight Leap Wireless Supports New Start-up Incubator 4New IVF Software MedITEX to be Presented at the ESHRE Exhibition 2MicroFluidic Systems (MFSI) Receives Patent for Method to Selectively Process Different Biological Cell Types for Analysis. 2
...
... Bio-Plex maintenance, calibration, and validation ... custom 96-well plate used for ... calibration procedures using the Bio-Plex ... plate is for use with ...
Mynox is intended for research use only. Mynox is used for the elimination of Mycoplasma and Acholeplasma in cell and virus cultures, and other biologicals....
... system holds 48 x 0.2 ml tubes, or ... independently excites fluorescence (470-500 nm) for each well, ... light (523-543 nm and 540-700 nm). The system ... Peltier heat pumps provide superb thermal performance. System ...
Biology Products: