Navigation Links
New technique tracks viral infections, aids development of antiviral drugs
Date:4/11/2011

WASHINGTON -- Scientists at the Naval Research Laboratory Center for Bio-Molecular Science and Engineering have developed a method to detect the presence of viruses in cells and to study their growth. Targeting a virus that has ribonucleic acid (RNA) as its genetic makeup, the new technique referred to as locked nucleic acid (LNA) flow cytometry-fluorescence in situ hybridization (flow-FISH), involves the binding of an LNA probe to viral RNA.

While individual parts of the technique have been developed previously, Drs. Kelly Robertson and Eddie Chang, in collaboration with researchers at the NRL Lab for Biosensors and Biomaterials, demonstrate for the first time that the combination of LNA probes with flow-FISH can be used to quantify viral RNA in infected cells. This also allows the scientists to monitor the changes in viral RNA accompanying antiviral drug treatment.

Once the probe is bound to the viral RNA inside mammalian cells, it is tagged with a fluorescent dye, then thousands of these tagged cells are measured rapidly by "flow cytometry" a method for counting and examining microscopic particles, such as cells and chromosomes, by suspending them in a stream of fluid and passing them by an electronic detection apparatus.

"The ability to rapidly measure thousands of cells for the presence of virus, sets this technique apart from currently used methods to monitor viral replication," said Robertson.

Traditionally, antibodies used to detect viruses must be produced and calibrated for each specific strain and are highly susceptible to viral mutations. Assays commonly used for quantifying viral loads and for drug development can be time consuming and rely on visible signs of cell damage, which is not produced in all viruses and can take long periods of time to occur.

Techniques such as quantitative reverse transcription-polymerase chain reaction (qRT-PCR), microarrays, and enzyme-linked immunosorbent assays (ELISAs)
'/>"/>

Contact: Daniel Parry
daniel.parry@nrl.navy.mil
202-767-2541
Naval Research Laboratory
Source:Eurekalert  

Page: 1 2

Related biology news :

1. Mapping the brain: New technique poised to untangle the complexity of the brain
2. DNA stretching -- A new technique being carried out at CIC microGUNE to detect illnesses
3. New imaging technique provides rapid, high-definition chemistry
4. New high-resolution carbon mapping techniques provide more accurate results
5. Combined molecular study techniques reveal more about DNA proteins
6. New non-surgical autopsy technique set to revolutionize post-mortem practice
7. NYU nursing-dental team receives grant to assess effectiveness of A1C diabetes screening technique
8. Ben-Gurion U. researchers develop techniques to manipulate plant adaption in arid climates
9. NIST technique controls sizes of nanoparticle clusters for EHS studies
10. New technique to see neurons of the deep brain for months at a time developed at Stanford
11. Technique allows researchers to identify key maize genes for increased yield
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
New technique tracks viral infections, aids development of antiviral drugs
(Date:7/11/2014)... evidence that under the normal circumstances, astrocytes ... maintain neuronal environment, and exhibit therapeutic and ... disease. Previous studies have found that nerve ... chemical induction have reduced viability, which produces ... Xiaodong Yuan, Kailuan General Hospital, Hebei United ...
(Date:7/11/2014)... , July 11, 2014 Research ... "International Biometrics Technology Market - Industry Analysis Size ... to their offering. http://photos.prnewswire.com/prnh/20130307/600769 ... and crimes has created a need for high level ... used for personnel authentication such as username and passwords, ...
(Date:7/11/2014)... Janeiro, Brazil- In the brains of all vertebrates, ... allows an electric or chemical signal to be ... synapses, which are the most abundant type of ... formation is crucial for learning, memory, perception and ... synapses critical for brain function. For instance, ...
Breaking Biology News(10 mins):International Biometrics Technology Market - Industry Analysis Size Share Growth Trends and Forecast to 2019 2Blame it on the astrocytes 2
... has made new links between 29 regions of the genome ... disease (IBD). The new findings increase the total number of ... to 99. The results point to several biological processes, ... the intestinal wall, which are likely to play an important ...
... With millions of people warding off winter,s chill with ... flags about the potential health effects of the smoke ... American Chemical Society,s (ACS,) journal, Chemical Research in ... the lungs from wood smoke may have several adverse ...
... they seem when it comes to fishsomething scientists at the ... Using modern genetic analysis, combined with traditional examination of morphology, ... three species of blenny in the genus Starksia ... in the scientific journal ZooKeys , Feb. 3. ...
Cached Biology News:Research links 29 genome regions with common form of inflammatory bowel disease 2Research links 29 genome regions with common form of inflammatory bowel disease 3Smithsonian scientists discover 7 new species of fish 2
(Date:7/12/2014)... Albany, New York (PRWEB) July 12, 2014 ... market analysis for various reagents used in ... biotechnology is driving the life science reagents ... this study include manufacturers and providers of ... Science Reagents Market Report: http://www.transparencymarketresearch.com/life-sciences-reagents-analytical-reagents.html . ...
(Date:7/11/2014)... , July 11, 2014 ... to announce the appointment of John P. Donoghue ... Biotech   in  Geneva . The ... Campus Biotech in Geneva , has ... recognised neuroscientists. John P. Donoghue , founder of ...
(Date:7/10/2014)... 2014 Product and process impurities ... abundance and are often “lost in the noise,” ... Join presenters Dr. Rowel Tobias, Senior Scientist, Protein ... Head of Quality at Nanotherapeutics, Inc., to lear ... speed detection and quantitation while achieving the required ...
(Date:7/10/2014)... Robert Harman, DVM, Founder and CEO of Vet-Stem, ... announce the relaunch of his highly informative blog, now named ... “ What are Stem Cells ?” Dr. Harman’s purpose ... in the basics of stem cell therapy so that pet ... of treatment when considering regenerative medicine. , A veterinarian by ...
Breaking Biology Technology:Transparency Market Research: Life Science Reagents Market- PCR Reagent Kits, Cell and Tissue Culture, Flow Cytometry, Electrophoresis and Chromatography 2Transparency Market Research: Life Science Reagents Market- PCR Reagent Kits, Cell and Tissue Culture, Flow Cytometry, Electrophoresis and Chromatography 3John P. Donoghue is Taking the Lead at the Wyss Center and is Appointed Professor at EPFL and University of Geneva 2John P. Donoghue is Taking the Lead at the Wyss Center and is Appointed Professor at EPFL and University of Geneva 3Novel Ms Techniques Speed Detection of Biopharmaceutical Product and Process Impurities, New Webinar Hosted by Xtalks 2Robert Harman, DVM Talks About What Stem Cells are in His Latest Blog Series for Vet-Stem, Inc. 2Robert Harman, DVM Talks About What Stem Cells are in His Latest Blog Series for Vet-Stem, Inc. 3
... platform offers multiple diagnostic modalities on a single ... to ... physicians and administrators, SAN DIEGO, March 28 ... of intravascular,ultrasound (IVUS), functional measurement (FM) and Optical Coherence,Tomography (OCT) ...
... /Xinhua-PRNewswire/ -- Tiens Biotech Group (USA),Inc. ("the Company" or ... financial results for the fourth quarter and twelve months ... of 2007 was $13.3 million compared to,$16.6 million for ... the fourth quarter of 2007 was $3.9 million, or ...
... -. Actavis Group, the,international generic pharmaceuticals company, ... approvals from the U.S. Food & Drug ... Distribution,of the products will commence immediately., ... strength, are the generic equivalent of Wellbutrin ...
Cached Biology Technology:Volcano Announces U.S. and European Launch of Rotational IVUS and Integrated FFR 2Volcano Announces U.S. and European Launch of Rotational IVUS and Integrated FFR 3Volcano Announces U.S. and European Launch of Rotational IVUS and Integrated FFR 4Volcano Announces U.S. and European Launch of Rotational IVUS and Integrated FFR 5Tiens Biotech Group (USA) Reports Fourth Quarter and Twelve-Month Results 2Tiens Biotech Group (USA) Reports Fourth Quarter and Twelve-Month Results 3Tiens Biotech Group (USA) Reports Fourth Quarter and Twelve-Month Results 4Tiens Biotech Group (USA) Reports Fourth Quarter and Twelve-Month Results 5Tiens Biotech Group (USA) Reports Fourth Quarter and Twelve-Month Results 6Tiens Biotech Group (USA) Reports Fourth Quarter and Twelve-Month Results 7Tiens Biotech Group (USA) Reports Fourth Quarter and Twelve-Month Results 8Tiens Biotech Group (USA) Reports Fourth Quarter and Twelve-Month Results 9Tiens Biotech Group (USA) Reports Fourth Quarter and Twelve-Month Results 10Actavis Receives Approval of Generic Wellbutrin SR(R) and Zyban(R) Tablets in the U.S. 2
The 50 ml Magnetic Separation Rack is designed to be used for small-scale separations using magnetic particles. ,Capacity: four 50 ml tubes. ,Magnets: Neodymium rare earth permanent magnets....
ChIP-GLAS kit for H10K-B microarray chips. Contains ChIP kit, GLAS kit, and hybridization kit. Additional reagents are required. Please see manual for details. Contains sufficient reagent for 20 reac...
...
...
Biology Products: