Navigation Links
New technique sheds light on RNA
Date:1/28/2013

ANN ARBOR, Mich. When researchers sequence the RNA of cancer cells, they can compare it to normal cells and see where there is more RNA. That can help lead them to the gene or protein that might be triggering the cancer.

But other than spotting a few known instigators, what does it mean? Is there more RNA because it's synthesizing too quickly or because it's not degrading fast enough? What part of the biological equilibrium is off?

After more than a decade of work, researchers at the University of Michigan Comprehensive Cancer Center have developed a technique to help answer those questions.

The method involves a compound called bromouridine, which can be used to tag or label newly created RNA. Researchers apply the bromouridine for 30 minutes then isolate the RNA to see where the new RNA was made. They call this process Bru-Seq.

On the other hand, the researchers can follow up the bromouridine labeling with a rinse with the chemical uridine for different periods of time. They call this BruChase-Seq because the uridine chases away the newly made RNA so they can look at how the RNA ages over the course of one hour, two hours or six hours. In other words, is the RNA degrading like it's supposed to?

"We can see the whole pattern of all the RNA that's synthesized and all the RNA that's stable vs. degrading. We can sort it out in terms of synthesis and stability and see if a particular RNA is more stable in the cancer cell than the normal cell or if it is taking longer to degrade in the cancer cell than in the normal cell," says study author Mats Ljungman, Ph.D., associate professor of radiation oncology at the U-M Medical School.

"With our technique, we're adding 10-fold more depth to the picture of how genes are expressed," he adds.

Ljungman is part of the Cancer Center's new Translational Oncology Program, which brings together cancer researchers from across the University of Michigan to speed the
'/>"/>

Contact: Nicole Fawcett
nfawcett@umich.edu
734-764-2220
University of Michigan Health System
Source:Eurekalert

Page: 1 2

Related biology news :

1. Novel technique reveals dynamics of telomere DNA structure
2. New technique could make cell-based immune therapies for cancer safer and more effective
3. VTT wins European innovation prize for a new bio-oil production technique
4. New technique to deliver stem cell therapy may help damaged eyes regain their sight
5. Fountain of youth technique rejuvenates aging stem cells
6. Safer spinach? Scientists technique dramatically reduces E. coli numbers
7. Surface analysis techniques for advanced materials enhance Mazovias research potential
8. Ancient foot massage technique may ease cancer symptoms
9. UT Arlington physics team demonstrates new power generation technique
10. New technique enables high-sensitivity view of cellular functions
11. Novel technique to produce stem cells from peripheral blood
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:1/22/2015)... 2015   EyeLock, Inc. , a market leader of iris-based ... Gerber to the new role of Senior Vice President ... development of mobile platforms and wearable solutions for EyeLock,s technologies. ... in the semiconductor industry to his role at EyeLock, with ...
(Date:12/24/2014)... , Dec. 23, 2014  Since its launch in December 2014, the ... eliminate the pain of trying to remember their usernames and ... fused to their smartphones. To assist people who have struggled ... company that created 1U and focuses on redefining identity, announced ...
(Date:12/22/2014)... Research and Markets ( http://www.researchandmarkets.com/research/fx4bg3/the_global ) ... Global Watermarking and Fingerprinting Markets" report to ... This insight provides an overview ... markets. Watermarking aims to control and monitor piracy ...
Breaking Biology News(10 mins):EyeLock Names Steve Gerber as Senior Vice President of Mobile and Wearables 21U Offers Best Solution to the Username / Password Dilemma: For FREE! 21U Offers Best Solution to the Username / Password Dilemma: For FREE! 3The Global Watermarking and Fingerprinting Markets 2
... common gene mutation found in acute myeloid leukaemia starts ... cooperate with a well-defined group of other mutations to ... critical steps are required to transform normal blood cells ... By charting the route towards cancer, the study identifies ...
... to handle, but when you chase it with prolonged oxygen ... last millions of years. Such was the situation with ... ago, when 90 percent of all marine animal species were ... and insect species on land. A massive amount of ...
... Hamilton schools has identified energy conservation measures that ... million annually. The audit was conducted by engineering ... measures, presented today to officials from the Hamilton-Wentworth ... School Board, range from recaulking windows, adding insulation ...
Cached Biology News:The gene processes that drive acute myeloid leukaemia 2The gene processes that drive acute myeloid leukaemia 3Algae, bacteria hogged oxygen after ancient mass extinction, slowed marine life recovery 2Algae, bacteria hogged oxygen after ancient mass extinction, slowed marine life recovery 3Algae, bacteria hogged oxygen after ancient mass extinction, slowed marine life recovery 4School energy audits find millions in potential energy savings 2School energy audits find millions in potential energy savings 3
(Date:1/22/2015)... Diego, California (PRWEB) January 22, 2015 ... Promotional Offer to their comprehensive portfolio of Eppendorf ... allows customers to purchase a Centrifuge 5424/5424 R and ... Bundle of either Eppendorf Research plus or Eppendorf Reference ...
(Date:1/22/2015)... January 22, 2015 Dr. Greg Leyer of ... annual Scripps Natural Supplements Pre-Conference seminar on probiotics in San ... an annual continuing education conference for health care professionals. This ... the topic of probiotics in health. Dr. Leyer spoke about ...
(Date:1/22/2015)... Selexis SA, a serial innovation company ... (RCBs) used for drug discovery to commercial manufacturing, announced ... Next-Generation Sequencing (NGS) data packages. The NGS ... by ensuring the integrity of the gene, validation of ...
(Date:1/22/2015)... NEW BRUNSWICK , Nueva Jersey , 22 ... Award for Biomedical Research abre hoy su llamada ... las personas cuya investigación científica ha hecho, o tiene ... de la salud humana. Las nominaciones se aceptarán hasta ...
Breaking Biology Technology:Eppendorf Announces Promotional Bundle Which Includes: Eppendorf Centrifuge 5424, 3-Pack of Pipettes, and Tips - Available Now at Pipette.com 2Selexis Generated Research Cell Banks Now Fully Sequenced Using Next-Generation Sequencing 2El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 2El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 3El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 4
... 2011 EdeniQ, Inc. , a California-based clean ... that it has licensed yeast technology from the USDA ... Foundation (WARF) associated with the fermentation of C5 sugars, ... culmination of the 3-year Cooperative Research and Development Agreement ...
... June 14, 2011 Arcion Therapeutics , a ... for chronic pain, today announced that data from the ... pain associated with painful diabetic neuropathy (PDN) will be ... meetings.  The first poster will be presented at the ...
... PAREXEL International Corporation (NASDAQ: PRXL ... Wells Fargo Healthcare Conference in Boston, MA.  James Winschel, Sr. ... presentation on PAREXEL at 2:00 p.m. ET on Wednesday, June ... will be available through the "Investors" section of ...
Cached Biology Technology:EdeniQ Licenses Yeast Technology from USDA 2Arcion Therapeutics to Present ARC-4558 Phase 2b Data for the Treatment of Pain Associated With Painful Diabetic Neuropathy at Upcoming Medical Meetings 2Arcion Therapeutics to Present ARC-4558 Phase 2b Data for the Treatment of Pain Associated With Painful Diabetic Neuropathy at Upcoming Medical Meetings 3PAREXEL International to Present at Wells Fargo Healthcare Conference 2
... (bluish-purple), DNA (blue), proteins (red) and acid polysaccharides ... traces: Ca ≤ 10 mg/kg Cd ≤ 5 mg/kg ... ≤ 5 mg/kg Fe ≤ 5 mg/kg K ≤ ... mg/kg Na ≤ 50 mg/kg Ni ≤ 5 mg/kg ...
BioGenex IHC Kits for Rodent Tissues are designed for immunohistochemistry on rodent and rabbit tissues that are closely related to the source of the primary antibody. This kit comes with rat seconda...
... The BioGenex Super Sensitive ... is a novel product ... non-biotin polymeric technology. This ... signal to noise ratio ...
... The dual-bay DNA Engine Dyad ... for DNA amplification (PCR). The chassis ... units (sample-holder, heat-pump assemblies), which hold ... Color display offers graphical programming with ...
Biology Products: