Navigation Links
New technique sheds light on RNA
Date:1/28/2013

ANN ARBOR, Mich. When researchers sequence the RNA of cancer cells, they can compare it to normal cells and see where there is more RNA. That can help lead them to the gene or protein that might be triggering the cancer.

But other than spotting a few known instigators, what does it mean? Is there more RNA because it's synthesizing too quickly or because it's not degrading fast enough? What part of the biological equilibrium is off?

After more than a decade of work, researchers at the University of Michigan Comprehensive Cancer Center have developed a technique to help answer those questions.

The method involves a compound called bromouridine, which can be used to tag or label newly created RNA. Researchers apply the bromouridine for 30 minutes then isolate the RNA to see where the new RNA was made. They call this process Bru-Seq.

On the other hand, the researchers can follow up the bromouridine labeling with a rinse with the chemical uridine for different periods of time. They call this BruChase-Seq because the uridine chases away the newly made RNA so they can look at how the RNA ages over the course of one hour, two hours or six hours. In other words, is the RNA degrading like it's supposed to?

"We can see the whole pattern of all the RNA that's synthesized and all the RNA that's stable vs. degrading. We can sort it out in terms of synthesis and stability and see if a particular RNA is more stable in the cancer cell than the normal cell or if it is taking longer to degrade in the cancer cell than in the normal cell," says study author Mats Ljungman, Ph.D., associate professor of radiation oncology at the U-M Medical School.

"With our technique, we're adding 10-fold more depth to the picture of how genes are expressed," he adds.

Ljungman is part of the Cancer Center's new Translational Oncology Program, which brings together cancer researchers from across the University of Michigan to speed the
'/>"/>

Contact: Nicole Fawcett
nfawcett@umich.edu
734-764-2220
University of Michigan Health System
Source:Eurekalert

Page: 1 2

Related biology news :

1. Novel technique reveals dynamics of telomere DNA structure
2. New technique could make cell-based immune therapies for cancer safer and more effective
3. VTT wins European innovation prize for a new bio-oil production technique
4. New technique to deliver stem cell therapy may help damaged eyes regain their sight
5. Fountain of youth technique rejuvenates aging stem cells
6. Safer spinach? Scientists technique dramatically reduces E. coli numbers
7. Surface analysis techniques for advanced materials enhance Mazovias research potential
8. Ancient foot massage technique may ease cancer symptoms
9. UT Arlington physics team demonstrates new power generation technique
10. New technique enables high-sensitivity view of cellular functions
11. Novel technique to produce stem cells from peripheral blood
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:10/16/2014)... challenged conventional thinking on how the bowel lining develops ... how bowel cancer starts. , The researchers produced evidence ... the ,crypts, that are a feature of the bowel ... bowel cancer development, a controversial finding as scientists are ... 3D imaging technologies, Dr Chin Wee Tan and Professor ...
(Date:10/15/2014)... Oct. 15, 2014 Sandata Technologies, LLC, ... and community-based care, today announced it released a ... Santrax® Electronic Visit Verification™ Solution (EVV™)  for Quality ... home health company founded in 1996 and has ... The study details the challenges ...
(Date:10/15/2014)... to bacterial pneumonia in female mice to an enzyme ... are naturally more resistant to respiratory infections than males. ... increased resistance to bacterial pneumonia in female mice is ... They also show that this enzyme is ultimately activated ... , The team, lead by Professor Lester Kobzik at ...
Breaking Biology News(10 mins):Cryptic clues drive new theory of bowel cancer development 2Sandata Announces Case Study with Quality Care Services, Inc. 2
... In our zest for cleanliness, have we permanently muddied ... from Arizona State University, in collaboration with federal partners,has ... Minnesota, finding widespread evidence of the presence of active ... and rivers. These products are a billion dollar ...
... species could soon follow, according to new research. Scientists have ... team has now carried out the first experiment to prove ... Society journal Biology Letters , the study shows how ... to become extinct. The University of Exeter team believes any ...
... the U.S. Department of Energy,s Ames Laboratory are helping ... tissue structures. The new method opens up new ... for biofuels research and crop genetics. "The ... Ames Laboratory faculty scientist heading up the project, funded ...
Cached Biology News:Antimicrobials from personal care products found in statewide survey of Minnesota's rivers and lakes 2Antimicrobials from personal care products found in statewide survey of Minnesota's rivers and lakes 3Antimicrobials from personal care products found in statewide survey of Minnesota's rivers and lakes 4Study proves that 1 extinction leads to another 2Finding new research frontiers in a single cell 2Finding new research frontiers in a single cell 3
(Date:10/22/2014)... 2014 WriteResult, LLC – a ... been working with a team of researchers from Yale ... Community Farming Collaborative to provide electronic questionnaire data collection ... study, which started in May, aims to evaluate the ... weekly exposure to and participation in urban agriculture to ...
(Date:10/22/2014)... SC (PRWEB) October 22, 2014 ... of its cardiovascular pharmacogenetics menu, which enables healthcare ... outcomes. With PCLS’s evidence-based results, healthcare providers ... and optimize their therapy, while minimizing risks for ... the U.S., according to the FDA [1] more ...
(Date:10/22/2014)... , Oct. 22, 2014 /PRNewswire/ - iCo Therapeutics ... today announced next steps for its Oral Amphotericin ... from its in vitro work involving ... iCo now plans to complete pre clinical studies ... initial Phase 1A clinical trial, utilizing approximately $700,000 ...
(Date:10/22/2014)... HONG KONG , Oct. 22, 2014 /PRNewswire/ ... announced today that rare disease expert John C. ... research. Dr. McKew brings more than two decades of ... the National Institutes of Health, Wyeth Research and Genetics ... will lead aTyr,s efforts to expand and translate its ...
Breaking Biology Technology:myPROpad™ Topic of Panel Discussion at the Yale Food Systems Symposium 2myPROpad™ Topic of Panel Discussion at the Yale Food Systems Symposium 3PCLS Announces Expansion of Cardiovascular Pharmacogenetic Testing Menu 2PCLS Announces Expansion of Cardiovascular Pharmacogenetic Testing Menu 3iCo Therapeutics Announces Advancement of Oral Amphotericin B Program 2iCo Therapeutics Announces Advancement of Oral Amphotericin B Program 3aTyr Pharma Appoints John C. McKew, Ph.D., as Vice President, Research 2aTyr Pharma Appoints John C. McKew, Ph.D., as Vice President, Research 3
... Senetek PLC,(OTC Bulletin Board: SNKTY), a Life Sciences ... science of healthy aging, today,announced that shareholders have approved ... Annual General Meeting of Shareholders held on,December 16, 2008. ... pleased with the results of the voting and we ...
... In a recent customer satisfaction,survey of over 30,000 readers ... by its customers as the "Top Of Class" winner ... who,subscribe to ADVANCE chose bioMerieux over other clinical laboratory ... "reliable,instruments," and "quick response time." , ...
... Senior Director, Commercial Development;Sam Zeller appointed Senior Director, Regulatory ... Mass., Dec. 17 Microbia Precision Engineering, Inc. (Microbia) ... team and bioproduct commercialization capabilities with the appointments of ... Sam Zeller as Senior Director of Regulatory Affairs. , ...
Cached Biology Technology:Senetek PLC Announces 2008 Annual General Meeting Voting Results 2Laboratory Customers Name bioMerieux 'Top Of Class' for Microbiology 2Microbia PE Bolsters Senior Management Team; Positions Company for Commercializing Proprietary Bioproducts 2Microbia PE Bolsters Senior Management Team; Positions Company for Commercializing Proprietary Bioproducts 3
... Genomic Labeling Systems are a high-performance labeling ... DNA samples for array-based Comparative Genomic Hybridization ... labeling formats, the BioPrime Plus Array CGH ... to your genomic labeling needs. Using ...
... effect inhibition assays, also known as CPE and ... supernatents; serum from PK studies and delivery formulations. ... as the quantity of interferon required per milliliter ... by 50%. All standards used in ...
Request Info...
...
Biology Products: