Navigation Links
New technique is quantum leap forward in understanding proteins
Date:12/23/2008

in a pair.

To visualize this computational challenge, think of a grid that is 100 residues tall, for the first protein, and 100 residues wide, from the second protein. The resulting 10,000 boxes in this grid represent all of the potential residue interactions, and the overall analysis forms a cube 2,500 blocks deep because there is a similar grid for each of the 2,500 protein pairings. Covariance can rank each of these 25 million blocks to identify the target residues that interact directly, along with those numerous indirect pairings that need to be winnowed away.

The innovative next step was for the UCSD group to feed this covariance data into a message-passing program. Over the course of about a week of computing, the program analyzed this seemingly unfathomable mass of information and in time identified patterns in the highest-ranking cubes. Continued analysis ultimately yielded predictions about which pairings were in fact direct interactions.

Because the two-component signaling system has been the focus of intense research efforts at Scripps Research and elsewhere, including extensive x-ray crystallography, many of the direct residue interactions had already been identified. That meant all-or-nothing results for the very first message-passing experiment. Either the technique would accurately identify the direct pairings or not.

The results came back overwhelmingly positive, and it was the culmination of a very long quest for Hoch. "It felt absolutely great," he says, "I thought, 'We finally got it! We got it and it works!'"

With a given protein binding site, on average, the message passing identified ten direct interactions accurately before giving a single false positive. Given that researchers can identify the active binding site for proteins by knowing as few as three directly interaction residues, this success rate is more than enough, for instance, to identify a new drug target. In the case of proteins that in
'/>"/>

Contact: Keith McKeown
kmckeown@scripps.edu
858-784-8134
Scripps Research Institute
Source:Eurekalert

Page: 1 2 3 4

Related biology news :

1. New technique can be breakthrough for early cancer diagnosis
2. University of Leicester scientists discover technique to help friendly bacteria
3. New technique reveals subtle force-induced changes in biomolecules conformation
4. New magnetic separation technique might detect multiple pathogens at once
5. New technique captures chemical reactions in a single living cell at unprecedented resolution
6. Software Techniques Inc. Selects M2SYS Fingerprint Software to Meet Continuous Growth and Demand for Desktop Biometric Time & Attendance Solutions
7. New X-ray technique targets terrorists and tumors
8. Technique controls nanoparticle size, makes large numbers
9. New technique could dramatically lower costs of DNA sequencing
10. New techniques create butanol
11. New radar satellite technique sheds light on ocean current dynamics
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/31/2015)... SHENZHEN , Chine, 31 juillet 2015 /PRNewswire/ ... (ICG-10, www.icg-10.org ) sera organisée par le ... Shenzhen en Chine. ... année. Depuis son inauguration en 2006, l,ICG est ... influentes dans le domaine des « omiques » et c,est ...
(Date:7/31/2015)... , 31. Juli 2015 Die 10. internationale ... von BGI veranstaltet und findet vom 22. bis ... , statt. Die Konferenz ... ihrem Start 2006 ist die ICG weltweit zu ... geworden. Sie ist eines der dynamischsten, enthusiastischsten und ...
(Date:7/31/2015)... The 10 th International Conference on Genomics ... 22-25, 2015, in Shenzhen , ... 10 th anniversary this year. Since its inauguration in ... annual meetings in the ,omics, fields, and is one of ... ICG-10 focuses on recent breakthroughs and advances in ,omics, ...
Breaking Biology News(10 mins):La 10e International Conference on Genomics (ICG-10) doit s'ouvrir en octobre 2La 10e International Conference on Genomics (ICG-10) doit s'ouvrir en octobre 3Die 10. internationale Konferenz zu Genomik (ICG-10) beginnt im Oktober 2Die 10. internationale Konferenz zu Genomik (ICG-10) beginnt im Oktober 3The 10th International Conference on Genomics (ICG-10) to Open in October 2
... Darwin based his groundbreaking theory of natural selection on ... key to evolution. Some individuals are better adapted ... likely to survive and pass on their genes to ... the first place still poses somewhat of a puzzle ...
... Springer to publish its official English-language journal, Human Cell ... published at Springer in early 2011. The journal was ... as a forum for international research on all aspects of ... of studies using ES/iPS cells or tissue stem cells derived ...
... study shows for the first time that the three ... disrupt a molecular network that includes several important genes ... research was led by investigators at the Ohio State ... Hospital and Richard J. Solove Research Institute (OSUCCC ...
Cached Biology News:Evolution by mistake 2Evolution by mistake 3Evolution by mistake 4Human Cell joins Springer's publishing program 2Molecular network influences development of chronic lymphocytic leukemia 2
(Date:8/27/2015)... ... August 27, 2015 , ... Inc. Magazine released its annual list of the ... 1,361 in growth for the three years through 2014. Being named to the Inc. ... organizations in the country. , “We are thrilled to make the Inc. 5000 ...
(Date:8/26/2015)... ... August 26, 2015 , ... MediVet ... a second US laboratory is to open in Manhattan, Kansas in early October, ... and long-term growth of research and development through collaboration with researchers from Kansas ...
(Date:8/26/2015)...  The Diabetes Research Institute (DRI), a Center ... School of Medicine, announced today that the first ... the first time a novel transplant technique for ... Phase I/II study builds upon decades of progress ... first step toward the development of the DRI ...
(Date:8/26/2015)... Aug. 26, 2015 China Cord Blood Corporation ... leading provider of cord blood collection, laboratory ... services, today announced that the Company has been informed ... entity wholly owned by Mr. Yuen Kam, chairman of ... the outstanding shares (the "Shares") of  Excellent China Healthcare ...
Breaking Biology Technology:iLab Solutions Named to 2015 Inc. 5000 List 2iLab Solutions Named to 2015 Inc. 5000 List 3MediVet Biologics Animal Health Secures Additional Laboratory Facility in Manhattan, Kansas 2MediVet Biologics Animal Health Secures Additional Laboratory Facility in Manhattan, Kansas 3Diabetes Research Institute Successfully Transplants First Patient In Pilot BioHub Trial 2Diabetes Research Institute Successfully Transplants First Patient In Pilot BioHub Trial 3Diabetes Research Institute Successfully Transplants First Patient In Pilot BioHub Trial 4China Cord Blood Corporation Receives Notice of Transactions Regarding its 7% Senior Convertible Notes Due 2017 2China Cord Blood Corporation Receives Notice of Transactions Regarding its 7% Senior Convertible Notes Due 2017 3
... Techware , a broadband wireless Internet provider, has announced that ... , ,The company, which started the build out in Germantown, ... capable of providing speeds up to 14 Mbps ... business users in Butler and the surrounding area. , ...
... failed in its bid to lure a Georgia stem cell company ... stem cell kits for researchers, apparently has found the financial backing ... of the University of Georgia . , ,Dick Leazer, a ... will remain in Georgia, despite an incentive package offered by the ...
... It's been a long time since Wisconsin landed a federal research ... Forest Products Laboratory came to Madison in 1910 when Gifford ... was pioneering scientific forestry. The National Wildlife Health Center , ... as scientists there engage in a race against bird flu. , ...
Cached Biology Technology:Georgia stem cell company likely to stay put 2Federal bioenergy lab will give Wisconsin a chance to shine 2Federal bioenergy lab will give Wisconsin a chance to shine 3Federal bioenergy lab will give Wisconsin a chance to shine 4
...
St. Louis encephalitis Immunogen: St. Louis encephalitis. Storage: -20 C, Avoid Freeze/Thaw Cycles...
Mouse Anti-Gemin2...
5-bromo-2'-deoxyuridine (BrdU) Cell Biology Cell Proliferation Bromodeoxyuridine (BrdU) and Related Products...
Biology Products: