Navigation Links
New technique for identifying gene-enhancers
Date:3/24/2014

An international team led by researchers with the Lawrence Berkeley National Laboratory (Berkeley Lab) has developed a new technique for identifying gene enhancers - sequences of DNA that act to amplify the expression of a specific gene in the genomes of humans and other mammals. Called SIF-seq, for site-specific integration fluorescence-activated cell sorting followed by sequencing, this new technique complements existing genomic tools, such as ChIP-seq (chromatin immunoprecipitation followed by sequencing), and offers some additional benefits.

"While ChIP-seq is very powerful in that it can query an entire genome for characteristics associated with enhancer activity in a single experiment, it can fail to identify some enhancers and identify some sites as being enhancers when they really aren't," says Diane Dickel, a geneticist with Berkeley Lab's Genomics Division and member of the SIF-seq development team. "SIF-seq is currently capable of testing only hundreds to a few thousand sites for enhancer activity in a single experiment, but can determine enhancer activity more accurately than ChIP-seq and is therefore a very good validation assay for assessing ChIP-seq results."

Dickel is the lead author of a paper in Nature Methods describing this new technique. The paper is titled "Function-based identification of mammalian enhancers using site-specific integration." The corresponding authors are Axel Visel and Len Pennacchio, also geneticists with Berkeley Lab's Genomics Division. (See below for a complete list of authors.)

With the increasing awareness of the important role that gene enhancers play in normal cell development as well as in disease, there is strong scientific interest in identifying and characterizing these enhancers. This is a challenging task because an enhancer does not have to be located directly adjacent to the gene whose expression it regulates, but can instead be located hundreds of thousands of DNA base p
'/>"/>

Contact: Lynn Yarris
lcyarris@lbl.gov
510-486-5375
DOE/Lawrence Berkeley National Laboratory
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. New synthetic biology technique boosts microbial production of diesel fuel
2. New genetic bar code technique establishes ability to derive DNA information from RNA
3. Early detection techniques offer hope for improved outcomes in lung cancer patients
4. Scientists develop new technique that could improve heart attack prediction
5. New rearing system may aid sterile insect technique against mosquitoes
6. Its a trap! New laboratory technique captures microRNA targets
7. New screening technique yields elusive compounds to block immune-regulating enzyme
8. New stem cell technique promises abundance of key heart cells
9. Evaluation of microscopy techniques may help scientists to better understand ancient plants
10. New technique allows simulation of noncrystalline materials
11. Transgenic technique to eliminate a specific neural circuit of the brain in primates
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
New technique for identifying gene-enhancers
(Date:6/30/2015)... NEW YORK , June 30, 2015 To ... biometric security provider HYPR Corp. announced today the addition of ... will serve as board advisor and David Raviv ... to the team underscore HYPR Corp.,s commitment to providing the ... , Dimitri Sirota co-founded Layer 7 Technologies, a ...
(Date:6/29/2015)... -- Research and Markets( http://www.researchandmarkets.com/research/zj7h4f/latin_america ) ... Biomedical Sensors Market - Growth, Trends & Forecasts (2014-2020)" ... America Biomedical Sensors market is estimated at $0.65 billion ... period 2014-2020 The near future will bring ... of each individual. These sensors can be set to ...
(Date:6/25/2015)... June 25, 2015 According ... Type (Swipe & Area), Technology, Material (Optical Prism, ... Commercial Security & Others) & Geography - Global ... the said market is expected to reach $14,500.07 ... Browse 76 market Tables and ...
Breaking Biology News(10 mins):HYPR Corp. Expands Biometric Authentication Team with Enterprise All Stars 2HYPR Corp. Expands Biometric Authentication Team with Enterprise All Stars 3Latin America Biomedical Sensors Market Report 2015-2020 2Fingerprint Sensors Market Worth $14,500.07 Million by 2020 2Fingerprint Sensors Market Worth $14,500.07 Million by 2020 3Fingerprint Sensors Market Worth $14,500.07 Million by 2020 4
... for Integrative Legume Research (CILR) has lodged a complete ... CILR researchers screened legumes (plants which obtain useable nitrogen ... and they identified a number of compounds which could ... tumours. Without an adequate blood supply tumours stop growing ...
... can retain a good memory even in your twilight years ... believe they can control their memory are more likely to ... march of time. These are the conclusions of a new ... , The study demonstrates a link between actual cognitive functioning ...
... differences between humans and chimpanzees are due more to ... themselves, researchers from Yale, the University of Chicago, and ... the 9 March 2006 issue of the journal ... for a 30-year-old theory, proposed in a classic paper ...
Cached Biology News:Legume compounds may help cancer treatment 2Confidence in memory performance helps older adults remember 2Most human-chimp differences due to gene regulation ?not genes 2Most human-chimp differences due to gene regulation ?not genes 3
(Date:7/30/2015)...   GenoSpace , a leading provider of ... of genomic and other biomedical data, today announced ... company as Vice President of Sales and Marketing. ... "GenoSpace partners with its customers to ... explore complex sets of genomic, phenotypic and other ...
(Date:7/30/2015)... July 30, 2015   Aratana Therapeutics, Inc ... company focused on the licensing, development and commercialization ... announced that its strategic partner VetStem Biopharma, Inc. ... study of AT-016, an adipose-derived allogeneic stem cell ... of osteoarthritis pain in dogs. The ...
(Date:7/30/2015)... , July 30, 2015  Discovery Laboratories, Inc. ... has received the second $1.0M tranche under a ... (SBIR) grant valued at up to $3.0 million ... KL4 surfactant as a potential medical countermeasure to ... Labs was awarded an initial $1.0 million under ...
(Date:7/29/2015)... According to a new market research report, ... , published by Mordor Intelligence, the Global market is expected ... 2020, with North America being the ... global market size. The Global market for Stem Cells is ... period of (2015-2020).      (Logo: http://photos.prnewswire.com/prnh/20150428/740799) , ...
Breaking Biology Technology:GenoSpace Introduces Matthew Fischer as Vice President of Sales and Marketing 2Aratana Therapeutics Announces Positive Pilot Field Study for AT-016 2Aratana Therapeutics Announces Positive Pilot Field Study for AT-016 3Aratana Therapeutics Announces Positive Pilot Field Study for AT-016 4Aratana Therapeutics Announces Positive Pilot Field Study for AT-016 5Discovery Labs Receives $1.0 million under an SBIR Grant in Continuing Support of the Development of Aerosolized KL4 Surfactant to Address Radiation-Induced Lung Injury 2Discovery Labs Receives $1.0 million under an SBIR Grant in Continuing Support of the Development of Aerosolized KL4 Surfactant to Address Radiation-Induced Lung Injury 3Asia Pacific to Dominate the Global Stem Cell Market - Vericel Corporation, Ocata Therpeutics, Athersys Inc., Cryo-Cell International, Geron Corporation, StemCells, Inc. Among the Major Players 2Asia Pacific to Dominate the Global Stem Cell Market - Vericel Corporation, Ocata Therpeutics, Athersys Inc., Cryo-Cell International, Geron Corporation, StemCells, Inc. Among the Major Players 3
... and health care clearing houses April 21, 2005 is ... prepare to become compliant with the HIPAA (Health Insurance ... that have to comply with HIPAA are called covered ... and Human Services and the Centers for Medicare ...
... , a Waukesha-based provider of supply chain technology, ... Polaris Industries . Minnesota-based Polaris, a manufacturer of ... garments and accessories business to RedPrairie. , ,RedPrairies ... DLx Slotting, are expected to improve Polaris replenishment ...
... isnt worried about attracting good workers to Procter & Gambles ... in the machinery, equipment and technology used by those workers ... that produce Charmin and Bounty and Puffs have increased 50 ... Scribner, manager of the companys Green Bay plant. The last ...
Cached Biology Technology:The HIPAA Security Regulation: The Journey to Compliance 2The HIPAA Security Regulation: The Journey to Compliance 3To attract investment, convince manufacturers that regulatory reform is real 2To attract investment, convince manufacturers that regulatory reform is real 3
alpha N-catenin (C-19)...
... AFFINITY PURIFIED GOAT ... ANTIBODY is conjugated with ... (RZ>3.0). The antibody ... solid-phase human, rat, fetal ...
Kanamycin is used for selection of resistant bacteria. C18H36N4O11xH2SO4. Contains 10 mg/ml kanamycin (base) utilizing kanamycin sulfate in 0.85% saline....
...
Biology Products: