Navigation Links
New technique enables study of 'challenging' proteins
Date:11/14/2011

Researchers from Hull, Bristol and Frankfurt have shown that a new technique for identifying molecular structure can be used effectively on small samples of biological proteins, particularly proteins that are targeted for drug development.

The technique, an enhanced form of nuclear magnetic resonance (NMR) spectroscopy, could enable the structure of a protein to be identified within hours, rather than weeks or months, radically speeding up the process of drug discovery. The findings are published online in the Journal of the American Chemical Society.

Dr Mark Lorch from the University of Hull, who led the research, explains: "Membrane proteins are important targets for the pharmaceutical industry, but they're very difficult to create in large quantities. For some, NMR isn't feasible at all, but even when it is, only small amounts of data can be gained from each small sample, which makes the whole process of identifying the structure very time consuming and expensive.

"Using this technique, we were able to get significant structural data from a small sample of a protein in just 20 hours of NMR time. This is the first time the technique has been shown to work on the size of sample that can be realistically created from any biological protein."

The researchers, from the Universities of Hull, Bristol and Goethe University, used a method known as dynamic nuclear polarisation (DNP), which boosts the number of nuclei that can be measured through NMR and so increases the signal picked up from the protein.

Although DNP has been used before on large sample sizes of well-studied proteins, the researchers are the first to show its effectiveness in studying a more challenging protein, opening the door to the study of biological samples that are currently inaccessible to conventional NMR.

The study focused on the Sec translocon protein, which transports other proteins either across or into biological membranes. This process is triggered when a signal peptide called LamB binds with Sec translocon and the researchers wanted to identify structural information on how the two interact. This would have been impossible through traditional NMR, as the signal peptide makes up such a small part of the sample to be studied. However, using DNP to enhance the signal from the peptide, the researchers were able to get significant information in a very short period of time.


'/>"/>

Contact: Abigail Chard
abigail@campuspr.co.uk
44-113-357-2100
University of Hull
Source:Eurekalert

Related biology news :

1. Novel technique switches triple-negative breast cancer cells to hormone-receptor positive cells
2. New membrane lipid measuring technique may help fight disease
3. Novel technique uses RNA interference to block inflammation
4. New technique elucidates dynamics of plant cell metabolites
5. NYU Langone experts find MRI techniques can detect early osteoarthritis
6. U of M researchers use improved imaging technique; discover a better approach to diagnosing epilepsy
7. Life scientists use novel technique to produce genetic map for African Americans
8. Cutting-edge imaging techniques for neuroscientists available in latest laboratory manual
9. New technique advances bioprinting of cells
10. New technique yields troves of information from nanoscale bone samples
11. New discovery -- copepods share divers weight belt technique with whales
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/16/2017)... , May 16, 2017   Bridge ... health organizations, and MD EMR Systems , ... development partner for GE, have established a partnership ... Portal product and the GE Centricity™ products, including ... EMR. These new integrations will ...
(Date:4/24/2017)... 2017 Janice Kephart , former ... Strategy Partners, LLP (IdSP) , today issues the ... Trump,s March 6, 2017 Executive Order: Protecting ... can be instilled with greater confidence, enabling the ... refugee applications are suspended by until at least ...
(Date:4/18/2017)... a global expert in SoC-based imaging and computing solutions, has developed ... the company,s hybrid codec technology. A demonstration utilizing TeraFaces ® , ... showcased during the upcoming Medtec Japan at Tokyo Big Sight April ... Vegas Convention Center April 24-27. ... Click here for an image of the M820 ...
Breaking Biology News(10 mins):
(Date:6/26/2017)... ... June 26, 2017 , ... Third Wave Bioactives, LLC announces ... Director, focused on leading new business development and ensuring quality customer experience. ... the food ingredient industry in technical, marketing and sales roles. “Brett’s background working with ...
(Date:6/23/2017)... Frederick, MD (PRWEB) , ... June 23, 2017 ... ... laboratory software solutions provider, announced the latest version of LimitLIS®, its rapidly growing ... designed to speed up user adoption, ensure installation integrity, and provide more customization ...
(Date:6/22/2017)... ... June 21, 2017 , ... Beaker, the industry’s pioneer ... the life sciences industry, today announces a strategic partnership with Alcami Corporation, a ... advantage of Beaker’s expertise in executive recruitment solutions, providing Alcami with access to ...
(Date:6/22/2017)... ... June 22, 2017 , ... ... designating infertility as a disease, bringing new hope for prospective parents who are ... annual meeting to back the World Health Organization’s designation in hopes of changing ...
Breaking Biology Technology: