Navigation Links
New technique could dramatically lower costs of DNA sequencing
Date:12/12/2007

CHAMPAIGN, Ill. Using computer simulations, researchers at the University of Illinois have demonstrated a strategy for sequencing DNA by driving the molecule back and forth through a nanopore capacitor in a semiconductor chip. The technique could lead to a device that would read human genomes quickly and affordably.

Being able to sequence a human genome for $1,000 or less (which is the price most insurance companies are willing to pay) could open a new era in personal medicine, making it possible to precisely diagnose the cause of many diseases and tailor drugs and treatment procedures to the genetic make-up of an individual.

Despite the tremendous interest in using nanopores for sequencing DNA, it was unclear how, exactly, nanopores could be used to read the DNA sequence, said U. of I. physics professor Aleksei Aksimentiev. We now describe one such method.

Aksimentiev and collaborators describe the method in a paper accepted for publication in the journal Nano Letters, and posted on the journals Web site.

Through molecular dynamics simulations, we demonstrate that back-and-forth motion of a DNA molecule in a nanopore capacitor 1 nanometer in diameter produces an electrostatic fingerprint that can be used to read the genetic sequence, said Aksimentiev, who also is a researcher at the Beckman Institute.

In the researchers simulations, performed at the universitys National Center for Supercomputing Applications, the nanopore capacitor consists of two conducting layers of doped silicon, separated by an insulating layer of silicon dioxide.

As DNA passes through the nanopore, the molecules electric field induces sequence-specific electrostatic potentials that can be detected at the top and bottom layers of the capacitor membrane.

A semiconductor device capable of reading the electrostatic potentials and decoding the genetic sequence is within the grasp of current technology, Aksimentiev said.

Nanometer pores in electronic membranes have been manufactured, and the voltage signals resulting from DNA movement through such pores have been recorded. The next big challenge, Aksimentiev said, is to minimize noise in the system, and reduce the speed of DNA molecules moving through the pore.


'/>"/>

Contact: James E. Kloeppel
kloeppel@uiuc.edu
217-244-1073
University of Illinois at Urbana-Champaign
Source:Eurekalert

Related biology news :

1. New technique can be breakthrough for early cancer diagnosis
2. University of Leicester scientists discover technique to help friendly bacteria
3. New technique reveals subtle force-induced changes in biomolecules conformation
4. New magnetic separation technique might detect multiple pathogens at once
5. New technique captures chemical reactions in a single living cell at unprecedented resolution
6. Software Techniques Inc. Selects M2SYS Fingerprint Software to Meet Continuous Growth and Demand for Desktop Biometric Time & Attendance Solutions
7. New X-ray technique targets terrorists and tumors
8. Technique controls nanoparticle size, makes large numbers
9. Newly created cancer stem cells could aid breast cancer research
10. Obesity and lack of exercise could enhance the risk of pancreatic cancer
11. Finding that 1-in-a-billion that could lead to disease
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/20/2017)... PMD Healthcare announces the release of its ... System (WMS), a remote, real-time lung health monitoring and ... is a Medical Device, Digital Health, and Chronic Care ... innovative solutions that empower people to improve their healthcare ... developed the first ever personal spirometer, Spiro PD, which ...
(Date:3/9/2017)... Australia , March 9, 2017 ... the prestigious World Lung Imaging Workshop at the University ... , was invited to deliver the latest data to ... globally recognised event brings together leaders at the forefront ... developments in lung imaging. "The quality ...
(Date:3/7/2017)... 2017   HireVue , the leading provider of ... the best talent, faster, today announced the additions of ... and Diana Kucer as Chief Marketing Officer ... team poised to drive continued growth in the company,s ... of record bookings in 2017. "Companies worldwide ...
Breaking Biology News(10 mins):
(Date:4/21/2017)... ... 2017 , ... Having worked on the design of the innovative Triton™ ... to top lab design architects from around the country at the Lab Design Conference ... and Engineering Greg Casey will be at the show, where they will highlight the ...
(Date:4/21/2017)... ... April 21, 2017 , ... Frederick Innovative Technology Center, ... emerging bio and technology start-ups, is hosting “Celebration Friday” (a festive gathering highlighting ... start with libations and networking at 3:30 p.m. at FITCI’s 4539 Metropolitan Court ...
(Date:4/20/2017)... 20, 2017  Eli Lilly and Company (NYSE: ... galcanezumab for the prevention of migraine at the American ... place April 22-28, 2017, in Boston ... at AAN, including safety and patient outcomes data for ... a reduction in monthly migraine headache days among patients ...
(Date:4/20/2017)... , April 20, 2017 /PRNewswire/ - Prometic Life Sciences Inc. ... new results at the International Liver Congress ("ILC") 2017 of ... in Amsterdam on the positive effects ... mouse model of obesity and metabolic syndrome. ... According to Dr. Lyne Gagnon, Vice-President of ...
Breaking Biology Technology: