Navigation Links
New technique allows study of protein folding, dynamics in living cells
Date:2/28/2010

CHAMPAIGN, Ill. A new technique to study protein dynamics in living cells has been created by a team of University of Illinois scientists, and evidence yielded from the new method indicates that an in vivo environment strongly modulates a protein's stability and folding rate, according to research accepted for publication in the journal Nature Methods and posted on the journal's Web site Feb. 28.

Martin Gruebele, the James R. Eiszner Professor of Chemistry at Illinois and corresponding author of the paper, says the method that he and his team of co-researchers engineered marks the first time anyone has been able to follow the real-time folding and unfolding of proteins outside of a test tube.

"This is the first experiment that allows us to observe the dynamics of a protein folding in a live cell," Gruebele said. "Now we have the capability of looking at how fast biological processes occur as a function of time."

To study the biomolecular dynamics inside of a single living cell, Gruebele and his team pioneered a hybrid method they've dubbed "Fast Relaxation Imaging," a technique that combines fluorescence microscopy and fast temperature jumps.

"It's a tool that combines two worlds: chemical dynamics, and the ability to study reactions as they occur; and biological environments, where cell biologists observe how reactions occur in cells," Gruebele said.

To achieve both a fast upward and downward temperature jump, programmed laser pulses are used to pre-heat, spike, plateau, cool and then finally stabilize the temperature in the cell and its aqueous medium at the final value. An inverted fluorescence microscope is used to observe and record what happens inside the cell, all of which takes place in the span of a few milliseconds.

The cells are usually heated to between 96 and 100 degrees Fahrenheit.

"It's like we give them a little bit of a fever," Gruebele said.

Gruebele says that although temperature jumps have be
'/>"/>

Contact: Phil Ciciora
pciciora@illinois.edu
217-333-2177
University of Illinois at Urbana-Champaign
Source:Eurekalert  

Page: 1 2 3 4

Related biology news :

1. Laser surgery technique gets new life in art restoration
2. Cameras of the future: heart researchers create revolutionary photographic technique
3. Virus-free technique enables Stanford scientists to easily make stem cells pluripotent
4. Enlisting a drug discovery technique in the battle against global warming
5. New edition of popular lab manual presents latest techniques for probing cellular dynamics
6. Using modern sequencing techniques to study ancient modern humans
7. Scripps research team develops technique to determine ethnic origin of stem cell lines
8. New RNA interference technique can silence up to 5 genes
9. New source of classical techniques for blood-based studies in the laboratory mouse
10. New forensic technique gives clues about sharks from bite damage
11. Technique finds gene regulatory sites without knowledge of regulators
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
New technique allows study of protein folding, dynamics in living cells
(Date:3/23/2015)...   HOYOS Labs , a leading global authentication ... the Company will demonstrate multiple biometrics-based technologies under its ... Connect:ID on March 23 through 25, 2015, in ... the IEEE Biometric Open Protocol Standard; free biometrics-based consumer ... control system. BOPS was invented by HOYOS Labs and ...
(Date:3/23/2015)... , Mar. 23, 2015 NXT-ID, Inc. (NASDAQ: NXTD ... on the growing mobile commerce market, announces its biometric payment ... campaign on CNBC television starting March 30 th . ... airing in New York markets. ... "We are excited about our new ad campaign following the ...
(Date:3/20/2015)... DUBLIN , Mar. 19, 2015 Research and Markets ... the "Hand Geometry - Global Strategic Business Report" ... worldwide markets for Hand Geometry in US$ Thousands. The report ... , Japan , Europe ... America , and Rest of World. Annual ...
Breaking Biology News(10 mins):HOYOS Labs to Showcase Host of Biometrics Technologies at Connect:ID in Washington, D.C. 2HOYOS Labs to Showcase Host of Biometrics Technologies at Connect:ID in Washington, D.C. 3NXT-ID's Wocket Smart Wallet to Launch New CNBC Regional TV Ad Campaign 2NXT-ID's Wocket Smart Wallet to Launch New CNBC Regional TV Ad Campaign 3Hand Geometry - Global Strategic Business Report 2015: An Essential Security Component for both Government & Enterprise Sector 2Hand Geometry - Global Strategic Business Report 2015: An Essential Security Component for both Government & Enterprise Sector 3Hand Geometry - Global Strategic Business Report 2015: An Essential Security Component for both Government & Enterprise Sector 4
... Giving antioxidants is recognised as one way of helping ... , The herbal medicine extracted from seeds of ... have antioxidant properties and research published this week in ... significantly lower the amount of sugar bound to haemoglobin ...
... air travel from countries where there is a serious influenza ... the infection, according to the findings of a study conducted ... journal PLoS Medicine. , Sometimes a new type of ... serious than is usually the case for flu. This happened, ...
... And yet, our "taste" for certain flavors and foods can change ... eating the same old thing. , Now, a new University ... the brain when we taste something we like, or develop a ... study used rats instead of people, it has direct implications for ...
Cached Biology News:Air travel and pandemic flu 2Tastes great! Study shows brain's response to pleasing -- and changing -- tastes 2Tastes great! Study shows brain's response to pleasing -- and changing -- tastes 3
(Date:3/26/2015)... "In America," the popular news television program that airs ... by legendary film actor James Earl Jones, has announced that ... segment. , Botany is commonly described as the scientific ... a bit more complicated than that. Scientists and other professionals ... from the smallest bacteria known to man to the largest ...
(Date:3/25/2015)... 2015 Agnition announced today that it ... Farmer, airing for the first time at 7:30 a.m. ... Farmer will explore the extremely important role soil microbes ... a patented Microbial Catalystâ„¢ technology that enhances soil and ... segment will air again on April 28. , A ...
(Date:3/25/2015)... March 25, 2015  S&P Capital IQ (MHFI) ... Stock Report coverage on Abattis Bioceuticals Corp . ... ATT): is a specialty biotechnology company with capabilities ... and marketing proprietary ingredients, bio-similar compounds, patented equipment ... North America . The company seeks ...
(Date:3/25/2015)... March 25, 2015 An increase of ... in all forms of food nutrients, regulatory changes and ... substantial rise in demand for natural astaxanthin over the ... to ramp up their capacities to satisfy the rising ... and synthetic source astaxanthin (used in nutraceuticals, aqua feed, ...
Breaking Biology Technology:Agnition to be Featured In Upcoming Episode of American Farmer on RFD-TV 2S&P Capital IQ Initiates Coverage on Abattis Bioceuticals Corp. in S&P Capital IQ Factual Stock Reports 2S&P Capital IQ Initiates Coverage on Abattis Bioceuticals Corp. in S&P Capital IQ Factual Stock Reports 3S&P Capital IQ Initiates Coverage on Abattis Bioceuticals Corp. in S&P Capital IQ Factual Stock Reports 4S&P Capital IQ Initiates Coverage on Abattis Bioceuticals Corp. in S&P Capital IQ Factual Stock Reports 5Global Astaxanthin Market to Reach Value of USD 1.1 billion by 2020, Expects Industry Experts in New Report Available at MarketPublishers.com 2
... step towards improving the design of future catalysts ... devices, researchers with the U.S. Department of Energys ... University of California (UC) at Berkeley, have successfully ... of gas-phase reactions on the microscale. , Under ...
... SCOTTSDALE, Ariz., Jan. 28 MedPredict has published,"Thought ... critical strategic insight for pharma and biotech companies ... treatments in this disease area., "Given the ... of approved,therapeutics for long-term disease control, it is ...
... 28 Cell Therapeutics, Inc.,(CTI) (Nasdaq and MTAX: CTIC) ... proxy proposal amending and restating the,Company,s articles of incorporation ... to 210 million and to increase the number of,authorized ... million., Louis A. Bianco, Chief Financial Officer at ...
Cached Biology Technology:Berkeley scientists bring MRI/NMR to microreactors 2Berkeley scientists bring MRI/NMR to microreactors 3Berkeley scientists bring MRI/NMR to microreactors 4Berkeley scientists bring MRI/NMR to microreactors 5New Report Tackles the Changing Landscape for Alzheimer's Diagnostics and Treatments 2Cell Therapeutics, Inc. (CTI) Shareholders Authorize Share Increase at Special Meeting of Shareholders 2
... plastic feeding tubes for rodent oral gavage ... risk of perforation and trauma. Plus they ... cleaning and the risk of cross-contamination. ... 20ga Length: ...
Adult Bovine Serum US Origin...
... atoms at the 9, 10, 12, and 13 ... internal standard for the quantification of 13(S)-HODE by ... incubation of linoleic acid with plant and mammalian ... adhesion of tumor cells to the endothelium at ...
...
Biology Products: