Navigation Links
New study overturns orthodoxy on how macrophages kill bacteria
Date:4/27/2009

derstand the role of an enzyme is to see what happens when it is absent, so the researchers looked at mutant S. typhimurium that had the genes for SodA, SodB, or both enzymes, deleted. Deleting the gene for SodA seemed to make no difference, but the SodB mutants were less able to survive and cause disease in a mouse. The double mutants were even more impaired. They were much, much less likely to survive in the mouse than bacteria with only the SodB gene missing. These findings "offer genetic proof" that both enzymes "are involved in the same process," Slauch said.

The fact that the bacterial mutants were less likely to survive in a mouse did not prove, however, that the missing enzymes were protecting the bacteria from ROS generated in the mouse macrophages, Slauch said.

"You get the same result if you grow these mutants in the laboratory in aerobic conditions," he said.

Furthermore, the SodA/SodB mutant bacteria were profoundly weakened even in a mouse that was unable to produce the potent ROS superoxide in its macrophages. These results suggest that the superoxide dismutases in the bacterial cytoplasm are most likely protecting the bacterium from its own, naturally occurring ROS, Slauch said.

In contrast, deleting the gene encoding the periplasmic superoxide dismutase, SodC, conferred the same defect regardless of whether the cytoplasmic SodA/SodB were present or absent, showing that its function is independent of the cytoplasm.

Moreover, strains lacking SodC were impaired only in the presence of superoxide produced in macrophages; there was no impairment in laboratory media or in mice lacking the ability to make superoxide.

This suggests that the superoxide and other reactive oxygen species are not making it from the macrophage into the bacterial cytoplasm, Slauch said.

"We conclude from all this data that the most sensitive target of ROS in the macrophages lies outside the cytoplasm," Slauc
'/>"/>

Contact: Diana Yates
diya@illinois.edu
217-333-5802
University of Illinois at Urbana-Champaign
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. Childhood obesity indicates greater risk of school absenteeism, Penn study reveals
2. A study by the MUHC and McGill University opens a new door to understanding cancer
3. Study begins to reveal clues to the cause and progression of sepsis
4. Clones on task serve greater good, evolutionary study shows
5. New study warns limited carbon market puts 20 percent of tropical forest at risk
6. New study examines how rearing environment can alter navigation
7. Study links cat disease to flame retardants in furniture and to pet food
8. New continent and species discovered in Atlantic study
9. Study shows link between alcohol consumption and hiv disease progression
10. Feeling hot, hot, hot: New study suggests ways to control fever-induced seizures
11. Study finds environmental tests help predict hospital-acquired Legionnaires disease risk
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
New study overturns orthodoxy on how macrophages kill bacteria
(Date:9/2/2014)... In the battle between our immune systems and ... bacteria-killing viruses known as phages. In a new study, ... Health, Haiti,s National Public Health Laboratory, and elsewhere, report ... their virulence in order to survive. Importantly, the study ... cholera,s mutational escape from phage predation occurs during human ...
(Date:9/2/2014)... the truth; everyone has a tipping point. We all want ... the benefit is great enough. Now, scientists have confirmed the ... , The result was published online this week in ... be honest, even if lying is beneficial," said Lusha Zhu, ... Virginia Tech Carilion Research Institute , where she works ...
(Date:9/2/2014)... Sam Kean stops by Reactions this week to debunk ... that, despite what "Shark Week" may lead you to ... http://youtu.be/KhFygIoW_MA . , Kean,s book, "The Disappearing Spoon: ... History of the World from the Periodic Table of ... 10-episode video series produced for the newly launched American ...
Breaking Biology News(10 mins):War between bacteria and phages benefits humans 2War between bacteria and phages benefits humans 3Scientists find possible neurobiological basis for tradeoff between honesty, self-interest 2Scientists find possible neurobiological basis for tradeoff between honesty, self-interest 3Scientists find possible neurobiological basis for tradeoff between honesty, self-interest 4
... Single person submersibles have been called in to help ... that may hold vital clues to the history of life ... environmental geochemist in the Faculty of Science, says the objects ... microbialites because they are covered with microbes. Some of these ...
... risks from the use of nanomaterials will be explored ... project supported by a $400,000 grant from the U.S.Department ... Nanomaterials are becoming more prevalent in our lives each ... less than one one-thousandth the width of a human ...
... Canadell from CSIRO and the Global Carbon Project, and ... of tons of carbon dioxide (CO2) absorbed annually by ... change mitigation worth hundreds of billions of dollars. ... challenges in quantifying changes in the size of those ...
Cached Biology News:Mini subs to probe odd structures in BC lake 2Growing use of nanomaterials spurs research to investigate possible downsides 2Growing use of nanomaterials spurs research to investigate possible downsides 3Tropical forest sustainability: A climate change boon 2
(Date:9/2/2014)...  Synthetic Biologics, Inc. (NYSE MKT: SYN), a ... targeting specific pathogens that cause serious infections and ... preclinical toxicology study of SYN-004. Per U.S. Food ... was required to move the Company,s lead anti-infective, ... effects of Clostridium difficile (C. difficile), ...
(Date:9/2/2014)... -- Culot brings life science and software ... BioData, producer of Labguru, the web-based research ... appointment of Louis Culot as the company,s ... and Business Development at BioDiscovery Inc., developer of genomics ... Previously, Culot worked for 17 years with CambridgeSoft ...
(Date:9/1/2014)... The Collaborative R&D Terms and Agreements ... understanding and unprecedented access to the collaborative R&D ... leading life science companies. , The report provides ... why companies enter collaborative R&D deals. , Understanding ... terms provides critical insight into the negotiation process ...
(Date:8/31/2014)... 2014 This is a professional ... the Epoxy Hardener industry in Global and ... the industry, including definitions, applications and industry chain ... analysis are provided with a focus on history, ... A comparison between the international and Chinese situation ...
Breaking Biology Technology:Synthetic Biologics to Initiate Clinical Trials of SYN-004 in 4Q 2014 to Prevent Potentially Deadly C. difficile Infections 2Synthetic Biologics to Initiate Clinical Trials of SYN-004 in 4Q 2014 to Prevent Potentially Deadly C. difficile Infections 3Synthetic Biologics to Initiate Clinical Trials of SYN-004 in 4Q 2014 to Prevent Potentially Deadly C. difficile Infections 4Synthetic Biologics to Initiate Clinical Trials of SYN-004 in 4Q 2014 to Prevent Potentially Deadly C. difficile Infections 5Louis Culot Appointed as BioData's CEO 2Collaborative R&D Terms & Agreements in Pharma, Biotech and Diagnostics Industry Analysis, Size, Share, Growth, Trends And Forecast Research Report by Researchmoz.us 2Collaborative R&D Terms & Agreements in Pharma, Biotech and Diagnostics Industry Analysis, Size, Share, Growth, Trends And Forecast Research Report by Researchmoz.us 3Collaborative R&D Terms & Agreements in Pharma, Biotech and Diagnostics Industry Analysis, Size, Share, Growth, Trends And Forecast Research Report by Researchmoz.us 4Epoxy Hardener Industry Development Trends in Global & China Regions Now Available at ReportsnReports.com 2Epoxy Hardener Industry Development Trends in Global & China Regions Now Available at ReportsnReports.com 3
... , , , , , , ... , 1691112, 100 g, ... Inserts/Product , Instructions , , German , ... version - 54 K , , US Material Safety , Data Sheets, ...
... , , , Template , ... outcome of the PCR. For instance, , large amounts of RNA ... of the PCR. Also, impure templates may contain polymerase inhibitors that , ... always use a purification product specifically designed to purify DNA such , ...
... , , , PCR principles , , ... , , Setting up the , ... , , Thermal , Cycling ... Enzymes , Choice of RT-PCR Enzymes , , ...
Cached Biology Technology:PCR reaction components 2PCR reaction components 3PCR reaction components 4PCR reaction components 5PCR reaction components 6Roche Diagnostics Corporation 2
... ,Schistosomal glutathione-S-transferase (GST) is commonly ... proteins in E. coli (1). The GSTTag ... production and in some cases the solubility ... a soluble, properly folded form, GSTTag fusion ...
... peptide whose sequence is from the C-terminal end of the mouse retinoic ... amino acids 429 to 448 of RAR*. The sequence of PEP-005 ... - S - P - S - S - V - E ... - S - P - L - L - Q (The ...
... applications for the use of small interfering ... via the RNA interference (RNAi) pathway have ... molecular biology fields. siRNA for experimental use ... or enzymatically, and then transfected into the ...
... The HybArray 12™ is an automated ... microarrays. The system automates both the hybridization ... onto glass slides. A single instrument can ... time in pairs and has multi-protocol software, ...
Biology Products: