Navigation Links
New source of classical techniques for blood-based studies in the laboratory mouse

The mouse is a standard laboratory model organism, but there are currently few resources that describe conventional techniques to analyze blood and blood-forming tissues in this species. A newly released set of compact and easy-to-use laboratory resources from Cold Spring Harbor Laboratory Press fills this gap. Mouse Hematology features step-by-step protocols for the preparation, enumeration, and microscopic examination of peripheral blood, bone marrow, and other hematopoietic tissues in the mouse. The laboratory manual is accompanied by a DVD with video demonstrations of the techniques and a poster of blood cell types for easy identification at the microscope.

Mouse Hematology was written by James J. Lee (Mayo Clinic Arizona) and two members of his lab, Michael P. McGarry and Cheryl A. Protheroe. All three have extensive experience employing hematological procedures in mice, and they wrote the book partly in response to numerous requests for help in preparing samples and identifying blood cell types. "Our goal here is to present standards and procedures for the examination of blood and blood-forming tissues of the laboratory mouse," the authors write in the preface of the book. "The described methodologies will allow for the morphological examination of blood, bone marrow, and/or hematopoietic tissues in research protocols and, in turn, will provide a greater understanding of mouse models of human disease."

The manual describes how to collect blood samples from adult mice and mouse pups; determine hematocrit and use a hemocytometer to count cells; and smear, fix, and stain blood films. Detailed protocols for performing bone marrow biopsies and for preparing bone marrow smears and suspensions for cytospin analysis are also provided. High-quality video demonstrations of most of the techniques are included on the DVD.

In addition to protocols, Mouse Hematology contains full-color images and detailed descriptions of the morphology of mature blood cells and their progenitors. The poster also presents examples of different blood cell types and will be a useful reference at the microscope.

This set of resources will be useful to laboratory scientists at all levels who work with mice to study hematopoiesis, stem cells, the immune system, and genetic diseases that affect the blood.


Contact: Ingrid Benirschke
Cold Spring Harbor Laboratory

Related biology news :

1. Pittsburgh scientists identify human source of stem cells with potential to repair muscle
2. UNH becomes first university in nation to use landfill gas as primary energy source
3. New partnership offers outsourced R&D in membrane biology
4. HortTalks, a valuable scientific resource
5. Reversing cognitive deficits: Injectable antibody may attack source of problem
6. The race for biofuels driving alternative sources of biomass
7. Space technology put into service for global water resources observations
8. Local sources major cause of US near-ground aerosol pollution
9. The power of multiples: Connecting wind farms can make a more reliable - and cheaper - power source
10. GBIF making the search for biodiversity research resources easier
11. Food source threatened by carbon dioxide
Post Your Comments:
Related Image:
New source of classical techniques for blood-based studies in the laboratory mouse
(Date:11/17/2015)... Pressure BioSciences, Inc. (OTCQB: PBIO) ("PBI" and ... of broadly enabling, pressure cycling technology ("PCT")-based sample preparation ... it has received gross proceeds of $745,000 from an ... "Offering"), increasing the total amount raised to date in ... are expected in the near future. ...
(Date:11/12/2015)... , Nov. 12, 2015  Arxspan has entered ... MIT and Harvard for use of its ArxLab ... management tools. The partnership will support the institute,s ... and chemical research information internally and with external ... used for managing the Institute,s electronic laboratory notebook, ...
(Date:11/10/2015)... 10, 2015  In this report, the ... of product, type, application, disease indication, and ... report are consumables, services, software. The type ... biomarkers, efficacy biomarkers, and validation biomarkers. The ... diagnostics development, drug discovery and development, personalized ...
Breaking Biology News(10 mins):
(Date:11/25/2015)... , Nov. 25, 2015  PharmAthene, Inc. (NYSE ... has adopted a stockholder rights plan (Rights Plan) in ... operating loss carryforwards (NOLs) under Section 382 of the ... --> PharmAthene,s use of its NOLs could ... change" as defined in Section 382 of the Code. ...
(Date:11/25/2015)... CITY , Nov. 25, 2015 /PRNewswire/ - Aeterna ... affirms that its business and prospects remain fundamentally ... , Zoptrex™ (zoptarelin doxorubicin) recently received DSMB recommendation ... to completion following review of the final interim ... Phase 2 Primary Endpoint in men with heavily ...
(Date:11/24/2015)... Malaysia , Nov. 24, 2015  Asia-Pacific ... contract research organisation (CRO) market. The trend of ... in lower margins but higher volume share for ... capacity and scale, however, margins in the CRO ... Organisation (CRO) Market ( ), finds ...
(Date:11/24/2015)... Mass. (PRWEB) , ... November 24, 2015 , ... ... to maintain healthy metabolism. But unless it is bound to proteins, copper is ... of Health (NIH), researchers at Worcester Polytechnic Institute (WPI) will conduct a systematic ...
Breaking Biology Technology: