Navigation Links
New source of classical techniques for blood-based studies in the laboratory mouse
Date:12/15/2009

The mouse is a standard laboratory model organism, but there are currently few resources that describe conventional techniques to analyze blood and blood-forming tissues in this species. A newly released set of compact and easy-to-use laboratory resources from Cold Spring Harbor Laboratory Press fills this gap. Mouse Hematology features step-by-step protocols for the preparation, enumeration, and microscopic examination of peripheral blood, bone marrow, and other hematopoietic tissues in the mouse. The laboratory manual is accompanied by a DVD with video demonstrations of the techniques and a poster of blood cell types for easy identification at the microscope.

Mouse Hematology was written by James J. Lee (Mayo Clinic Arizona) and two members of his lab, Michael P. McGarry and Cheryl A. Protheroe. All three have extensive experience employing hematological procedures in mice, and they wrote the book partly in response to numerous requests for help in preparing samples and identifying blood cell types. "Our goal here is to present standards and procedures for the examination of blood and blood-forming tissues of the laboratory mouse," the authors write in the preface of the book. "The described methodologies will allow for the morphological examination of blood, bone marrow, and/or hematopoietic tissues in research protocols and, in turn, will provide a greater understanding of mouse models of human disease."

The manual describes how to collect blood samples from adult mice and mouse pups; determine hematocrit and use a hemocytometer to count cells; and smear, fix, and stain blood films. Detailed protocols for performing bone marrow biopsies and for preparing bone marrow smears and suspensions for cytospin analysis are also provided. High-quality video demonstrations of most of the techniques are included on the DVD.

In addition to protocols, Mouse Hematology contains full-color images and detailed descriptions of the morphology of mature blood cells and their progenitors. The poster also presents examples of different blood cell types and will be a useful reference at the microscope.

This set of resources will be useful to laboratory scientists at all levels who work with mice to study hematopoiesis, stem cells, the immune system, and genetic diseases that affect the blood.


'/>"/>

Contact: Ingrid Benirschke
benirsch@cshl.edu
619-275-6021
Cold Spring Harbor Laboratory
Source:Eurekalert  

Related biology news :

1. Pittsburgh scientists identify human source of stem cells with potential to repair muscle
2. UNH becomes first university in nation to use landfill gas as primary energy source
3. New partnership offers outsourced R&D in membrane biology
4. HortTalks, a valuable scientific resource
5. Reversing cognitive deficits: Injectable antibody may attack source of problem
6. The race for biofuels driving alternative sources of biomass
7. Space technology put into service for global water resources observations
8. Local sources major cause of US near-ground aerosol pollution
9. The power of multiples: Connecting wind farms can make a more reliable - and cheaper - power source
10. GBIF making the search for biodiversity research resources easier
11. Food source threatened by carbon dioxide
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
New source of classical techniques for blood-based studies in the laboratory mouse
(Date:1/11/2017)... , Jan. 11, 2017  Michael Johnson, co-founder of Visikol Inc. ... Group, Inc., has been named to the elite "Forbes 30 Under ... one of 600 people in 20 fields nationwide to be recognized ... the 15,000 applicants were selected. ... He is currently a PhD candidate at Rutgers University. ...
(Date:1/4/2017)... the thousands of attendees at this year,s International Consumer Electronics Show (CES), ... measurement devices and services, will be featuring its new line of ULTRA ... special CES Exhibit Suite , the new upper arm and wrist ... WellnessConnected product platform.  Continue Reading ... ...
(Date:12/22/2016)... -- SuperCom (NASDAQ:   SPCB ... Public Safety, HealthCare, and Finance sectors announced today that Leaders in ... implement and deploy a community-based supportive services program to reduce recidivism ... further expanding its presence in the state. ... This new program, which is expected to ...
Breaking Biology News(10 mins):
(Date:1/20/2017)... , January 20, 2017 ... is one of leading causes of death worldwide. There ... the number of cancer related deaths increased gradually over ... in incidence rate of various cancers continues to drive ... research report by Global Market Insights, Inc. cancer biological ...
(Date:1/19/2017)... and HOUSTON , Jan. 19, ... today announced the formation of its Medical/Clinical Advisory ... and industry veterans who enhance the range and ... accelerates development of its novel prenatal diagnostic tests.  ... clinical and strategic guidance for the company,s product ...
(Date:1/19/2017)... ... January 19, 2017 , ... November Research Group, ... biopharmaceutical and medical device manufacturers and regulators, is proud to announce the worldwide ... email client designed to provide product vigilance departments with the flexibility and ease ...
(Date:1/19/2017)... ... January 18, 2017 , ... The American Medical Informatics ... its Data Sharing Policy. Specifically, the nation’s leading informatics experts, said data sharing ... existing policy. AMIA recommended that NIH earmark funding for researchers to produce and ...
Breaking Biology Technology: