Navigation Links
New source of classical techniques for blood-based studies in the laboratory mouse
Date:12/15/2009

The mouse is a standard laboratory model organism, but there are currently few resources that describe conventional techniques to analyze blood and blood-forming tissues in this species. A newly released set of compact and easy-to-use laboratory resources from Cold Spring Harbor Laboratory Press fills this gap. Mouse Hematology features step-by-step protocols for the preparation, enumeration, and microscopic examination of peripheral blood, bone marrow, and other hematopoietic tissues in the mouse. The laboratory manual is accompanied by a DVD with video demonstrations of the techniques and a poster of blood cell types for easy identification at the microscope.

Mouse Hematology was written by James J. Lee (Mayo Clinic Arizona) and two members of his lab, Michael P. McGarry and Cheryl A. Protheroe. All three have extensive experience employing hematological procedures in mice, and they wrote the book partly in response to numerous requests for help in preparing samples and identifying blood cell types. "Our goal here is to present standards and procedures for the examination of blood and blood-forming tissues of the laboratory mouse," the authors write in the preface of the book. "The described methodologies will allow for the morphological examination of blood, bone marrow, and/or hematopoietic tissues in research protocols and, in turn, will provide a greater understanding of mouse models of human disease."

The manual describes how to collect blood samples from adult mice and mouse pups; determine hematocrit and use a hemocytometer to count cells; and smear, fix, and stain blood films. Detailed protocols for performing bone marrow biopsies and for preparing bone marrow smears and suspensions for cytospin analysis are also provided. High-quality video demonstrations of most of the techniques are included on the DVD.

In addition to protocols, Mouse Hematology contains full-color images and detailed descriptions of the morphology of mature blood cells and their progenitors. The poster also presents examples of different blood cell types and will be a useful reference at the microscope.

This set of resources will be useful to laboratory scientists at all levels who work with mice to study hematopoiesis, stem cells, the immune system, and genetic diseases that affect the blood.


'/>"/>

Contact: Ingrid Benirschke
benirsch@cshl.edu
619-275-6021
Cold Spring Harbor Laboratory
Source:Eurekalert  

Related biology news :

1. Pittsburgh scientists identify human source of stem cells with potential to repair muscle
2. UNH becomes first university in nation to use landfill gas as primary energy source
3. New partnership offers outsourced R&D in membrane biology
4. HortTalks, a valuable scientific resource
5. Reversing cognitive deficits: Injectable antibody may attack source of problem
6. The race for biofuels driving alternative sources of biomass
7. Space technology put into service for global water resources observations
8. Local sources major cause of US near-ground aerosol pollution
9. The power of multiples: Connecting wind farms can make a more reliable - and cheaper - power source
10. GBIF making the search for biodiversity research resources easier
11. Food source threatened by carbon dioxide
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
New source of classical techniques for blood-based studies in the laboratory mouse
(Date:6/23/2017)... N.Y. and ITHACA, N.Y. ... ) and Cornell University, a leader in dairy research, ... with bioinformatics designed to help reduce the chances that ... With the onset of this dairy project, Cornell University ... Consortium for Sequencing the Food Supply Chain, a food ...
(Date:5/16/2017)... TEANECK, N.J. , May 16, 2017  Veratad ... leading provider of online age and identity verification solutions, ... the K(NO)W Identity Conference 2017, May 15 thru May ... Ronald Regan Building and International Trade Center. ... across the globe and in today,s quickly evolving digital ...
(Date:4/24/2017)... 24, 2017 Janice Kephart , ... Identity Strategy Partners, LLP (IdSP) , today issues ... President Trump,s March 6, 2017 Executive Order: ... vetting can be instilled with greater confidence, enabling ... all refugee applications are suspended by until at ...
Breaking Biology News(10 mins):
(Date:10/12/2017)... ... October 12, 2017 , ... AMRI, a global ... industries to improve patient outcomes and quality of life, will now be offering ... being attributed to new regulatory requirements for all new drug products, including the ...
(Date:10/11/2017)... ... October 11, 2017 , ... ... its endogenous context, enabling overexpression experiments and avoiding the use of exogenous expression ... guides is transformative for performing systematic gain-of-function studies. , This complement to ...
(Date:10/11/2017)... ... October 11, 2017 , ... ... it will be hosting a Webinar titled, “Pathology is going digital. Is your ... on digital pathology adoption best practices and how Proscia improves lab economics and ...
(Date:10/11/2017)... ... October 11, 2017 , ... Disappearing forests and increased emissions ... over 5.5 million people each year. Especially those living in larger cities are affected ... based in one of the most pollution-affected countries globally - decided to take action. ...
Breaking Biology Technology: