Navigation Links
New screening technique yields elusive compounds to block immune-regulating enzyme

gene from lab mice as an alternative way to study its functions was problematic, since mouse embryos can't survive without the protein.

Inspiration Close By

The inspiration for the new PRMT1-selective screening technique came from research performed in the neighboring laboratory of co-author Benjamin F. Cravatt III, who chairs the Scripps Research Department of Chemical Physiology. As reported in 2010 in the journal Nature, Cravatt and his team screened tens of thousands of human and mouse proteins for the presence of hyper-reactive cysteine amino acids, which almost certainly mark functional sites on those proteins. PRMT1 was found to be one of the reactive-cysteine-containing proteinsand all but one other, comparatively rare PRMT enzyme was known to lack that cysteine.

"We took that discovery a step further, and we were able to find a probe that specifically would recognize that cysteine in PRMT1," said Mowen, who was one of Cravatt's collaborators on the 2010 study.

She and her colleagues first verified that the reactive cysteine in PRMT1 is in the active site of the enzyme. They then found a fluorescent probe that would bind to that cysteine. If a test compound acted as an inhibitor by fastening to PRMT1's active site, it should interfere with the probe's binding, and the probe's fluorescence-based signal therefore should be lower. By contrast, if a test compound failed to bind to PRMT1's active site, the probe should bind normally and its signal should remain elevated.

"We were able to verify, using available non-specific inhibitors of PRMT enzymes, that they did indeed bind to PRMT1 and prevent the probe from binding, and that was the proof-of-concept that enabled us to go ahead with a screen," said Myles B. C. Dillon, a graduate student in Mowen's lab who was lead author of the study.

Exploring Libraries of Potential

Dillon and Mowen turned to collaborator Scripps Research Professo

Contact: Mika Ono
Scripps Research Institute

Page: 1 2 3

Related biology news :

1. Nanoscopic screening process to speed drug discovery
2. Oral rinses used for tracking HPV-positive head and neck cancers holds promise for cancer screening
3. Genetic risk factors may tailor prostate cancer screening approaches
4. Rural areas able to increase screening capacity for colorectal cancer
5. States expand newborn screening for life-threatening disorders
6. New technique for cancer screening
7. New high-throughput screening technique makes probing puzzling proteins possible
8. American College of Medical Genetics affirms importance of newborn screening dried blood spots
9. Colon cancer screening technique shows continued promise in new study
10. ESHRE launches international study of polar body screening
11. Pitt team finds molecule that regulates heart size by using zebrafish screening model
Post Your Comments:
Related Image:
New screening technique yields elusive compounds to block immune-regulating enzyme
(Date:7/2/2015)... Research and Markets( ) has ... Market by Application, Technology, Function & Geography - Global ... The next generation biometrics market is expected to ... 17.9% between 2015 and 2020 Travel & ... Safran SA ( France ), NEC ...
(Date:6/26/2015)... , June 26, 2015 ATL Technology, LLC, a ... with specialties in single use solutions, headquartered in ... Inc. (a California corporation with locations ... San Jose, Costa Rica ). This ... Rica with ATL Technology,s existing facility in ...
(Date:6/24/2015)... 24, 2015 This report provides market analysis ... six years. It contains an analysis of the drivers, ... their impact from the short, medium, and long term ... industry, market, and technology trends that currently, prevail in ... concerned authorities to efficiently manage the increasing marine traffic ...
Breaking Biology News(10 mins):Next Generation Biometrics Market 2015 - Global Forecast to 2020 with Safran, NEC, 3M Cogent, Fujitsu & Suprema Dominating the $24 Billion Market 2Next Generation Biometrics Market 2015 - Global Forecast to 2020 with Safran, NEC, 3M Cogent, Fujitsu & Suprema Dominating the $24 Billion Market 3ATL Technology Announces Acquisition of MedConx, Inc. and Expanded Global Footprint 2Automatic Identification System Market by Class, by Platform, by Application and by Geography - Global Forecasts & Analysis to 2014 - 2020 2
... a question asked by marine scientists from the Gulf of Mexico ... and marine habitat once it has been damaged or even killed? ... may be a cheap and simple solution that can be used ... was carried out by Dr Graham Forrester, from the University of ...
... French . , As part of the 2010 Congress ... led by Professor Barbara Layne of Concordia University, Montreal, and ... brought research in intelligent textiles to a new level. ... of interactive clothing whereby the body,s physical and emotional state ...
... --- The hunting strategy of a slender fish from the ... the metabolic cost of information with the metabolic cost of ... from Northwestern University,s McCormick School of Engineering and Applied Science ... move to get information, when should the animal spend more ...
Cached Biology News:Coral transplantation the simple and cheap solution to reef restoration 2Innovative research reawakens human memories through intelligent textiles 2The biomechanics of information 2
(Date:7/28/2015)... ... 2015 , ... The new Xsample 530 sample changer for vials can be used ... viscosities – from less than 12,000 mPas (internal air) up to 36,000 (external air) – ... catches the eye about Xsample 530 is its removable magazine, which is easily lifted off ...
(Date:7/28/2015)... 2015 People with a common form of ... and sometimes profound improvements in their hearing and understanding ... a new multicenter study led by specialists at NYU ... online ahead of print in the journal The Laryngoscope July ... in the United States implanted ...
(Date:7/28/2015)... ... 2015 , ... In terms of value, aluminium hydroxide production ... reached close to 1.84 million tonnes in the same year. As of 2013, ... region’s total aluminium hydroxide production; the country’s aluminium hydroxide production posted a 101.8% ...
(Date:7/28/2015)... -- Research and Markets ( ) has ... and Freezers Market - Global Industry Analysis, Size, ... report to their offering. ... been segmented based on applications: hospitals, research laboratories, ... others segment includes applications of biomedical refrigerators and ...
Breaking Biology Technology:New Moves in Automatic Sample Handling: Introducing Xsample 530 2Hybrid Cochlear Implants For Common Form Of Hearing Loss May Benefit Millions 2Hybrid Cochlear Implants For Common Form Of Hearing Loss May Benefit Millions 3EU27 Aluminium Hydroxide Market Analysed and Forecast by BAC Reports in New Research Study Available at 2EU27 Aluminium Hydroxide Market Analysed and Forecast by BAC Reports in New Research Study Available at 3Biomedical Refrigerators and Freezers Market Report - Global Industry Analysis 2015 2Biomedical Refrigerators and Freezers Market Report - Global Industry Analysis 2015 3
... Health and Human Services secretary and longtime Wisconsin governor, ... leaving his Cabinet post in January. , ,Thompson accepted ... Deloitte & Touche USA; law partner at Washington power ... of La Crosse-based Logistics Health Inc., a privately held ...
... state's right to charge taxes on Internet access and purchases. ... Democrat, has suggested charging sales taxes on more types of ... Wisconsin maintains its 14-year-old tax on access to the Internet, ... states from doing so. , ,The state levies a flat ...
... by state legislators who must pass judgment on a major ... second is, "How will it help Wisconsin and my ... for Discovery is providing some straightforward, and exciting, answers. , ... UW-Madison campus with half public money and half private gifts, ...
Cached Biology Technology:Tommy Thompson tackles the private sector in spades 2Discontent grows over Wisconsin's Internet taxes 2Discontent grows over Wisconsin's Internet taxes 3How the Institute for Discovery will help Wisconsin 2How the Institute for Discovery will help Wisconsin 3