Navigation Links
New retrieval method makes studying cancer proteins easier
Date:7/7/2010

WEST LAFAYETTE, Ind. - A Purdue University researcher can better retrieve specific proteins needed to study how cancer cells form by using a newly developed technique and synthetic nanopolymer.

W. Andy Tao, an assistant professor of biochemistry, said proteins can be mapped and analyzed to find ways to inhibit the processes that lead to cancer. But first those few proteins must be fished out of a sea of thousands of others.

Tao developed and patented the polymer-based metal-ion affinity capture, or PolyMAC. The synthetic nanopolymer isolates proteins and peptides that have undergone a process called phosphorylation that is highly associated with cancer, and a patented technique allows Tao to retrieve those proteins. Obtaining the information on these proteins is important for studying how to inhibit the processes that lead to cancer.

"You really want to capture these particular proteins, but there are so many different types of proteins around them," said Tao, whose findings were published in the early online version of the journal Molecular & Cellular Proteomics. "The target proteins are a thousand times lower in amount than other proteins. They are difficult to study without the capturing step."

Normal cells grow, divide and eventually die. But cancer cells continue to grow and do not die. Tao said phosphorylation - in which a type of enzyme called a kinase attaches to and catalyzes a protein on a cell - is thought in many cases to be responsible for creating cancer cells.

Tao's nanopolymer is water-soluble and has titanium ions on its surface, which bind with phosphorylated proteins and peptides contained in a solution. The polymer also has a chemical group attached that is reactive and attached to small beads, which allow Tao to retrieve the polymers.

"Once you put the nanopolymer in the solution, you have to retrieve them, so we put a handle on the polymer so we can grab on to it and fish it out of the solution," Tao said.

In laboratory tests, Tao's nanopolymer and retrieval technique isolated about twice as many proteins that had been phosphorylated by an enzyme highly expressed in certain leukemia cells but absent in metastatic breast cancer cells.

Tao is now seeking opportunities to get the polymer and technique into wider use to aid in the development of new cancer drugs.

"This technique is very useful and can be used widely in research for cancer as well as infectious diseases," Tao said.


'/>"/>

Contact: Brian Wallheimer
bwallhei@purdue.edu
765-496-2050
Purdue University
Source:Eurekalert

Related biology news :

1. Commercial aquatic plants offer cost-effective method for treating wastewater
2. UC Riverside biochemists devise method for bypassing aluminum toxicity effects in plants
3. New lab manual focuses on essential methods for purifying and characterizing proteins
4. Deep biosphere research points to new methods for recovering petroleum
5. Fingerprinting method tracks mercury emissions from coal
6. Iowa State researcher develops new treatment method for canine eye diseases
7. New method provides panoramic view of protein-RNA interactions in living cells
8. Cold Spring Harbor Protocols features organ and cell culture methods
9. Engineer to present leak-proof method for carbon dioxide storage at international conference
10. Pitt researchers use fluorescence to develop method for detecting mercury in fish
11. New holographic method could be used for lab-on-a-chip technologies
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/8/2017)... About Voice Recognition Biometrics Voice recognition biometrics ... a stored voiceprint template. Acoustic features of an ... are compared to distinguish between individual voices. Voice ... PCs already have a microphone and can authenticate ... are most likely to be deployed in telephone-based ...
(Date:2/8/2017)... 7, 2017 Report Highlights The ... from $8.3 billion in 2016 at a compound annual ... Report Includes - An overview of the global ... with data from 2015 and 2016, and projections of ... of the market on the basis of product type, ...
(Date:2/6/2017)... 2017 According to Acuity Market Intelligence, ... authorities to continue to embrace biometric and digital ... Automated Border Control (ABC) eGates and 1436 Automated ... than 163 ports of entry across the globe. ... a combined CAGR of 37%. APC Kiosks reached ...
Breaking Biology News(10 mins):
(Date:2/23/2017)... Maryland (PRWEB) , ... February ... ... PathSensors, Inc., announced today that in a published evaluation of multiple immunoassay-based ... a U.S. Department of Energy Laboratory, PathSensors’ CANARY® biosensor threat detection technology ...
(Date:2/23/2017)... , Feb. 23, 2017 Aviva Systems ... announced the acquisition of GenWay Biotech Incorporated, a ... service and product offering for both the research ... facilitate growth and enhance capabilities for both entities. GenWay,s ... ELISA assays will nicely complement ASB,s objective to ...
(Date:2/22/2017)... ... February 22, 2017 , ... Park Systems , a leader in Atomic ... all SPIE attendees and Park customers on Feb. 27, 2017 from 12-2pm ... Jose Convention Center. The luncheon will feature a talk on Automated AFM for ...
(Date:2/22/2017)... ... February 22, 2017 , ... LabRoots ... scientists from around the world, is pleased to announce the 2nd annual Precision ... 2017. This premier, online-only conference focused on the development and advancements in precision ...
Breaking Biology Technology: