Navigation Links
New resource to unlock the role of microRNAs
Date:8/7/2011

A new resource to define the roles of microRNAs is announced today in Nature Biotechnology. The resource, called mirKO, gives researchers access to tools to investigate the biological role and significance for human health of these enigmatic genes.

mirKO is a "library" of mutant mouse embryonic stem (ES) cells in which individual, or clustered groups of microRNA genes, have been deleted. Using these tools researchers can create cells or mice lacking specific microRNAs, study expression using fluorescent markers, or inactivate the gene in specific tissues or at specific times in development. This is the first mammalian microRNA knockout resource; the only other comprehensive resource of mutated microRNA genes is that for the nematode worm.

microRNAs first named only ten years ago are encoded by more than 500 genes that are predicted to regulate about one third of protein-coding genes. Consisting of short stretches of 21 to 23 nucleotides, microRNAs act by interfering with the activity of messenger RNAs. Studies over the past five years have shown that microRNAs are likely to play important roles in disease such as cancer and disorders of the heart, the immune system and auditory systems.

"We have generated a resource of microRNA knockout alleles in mouse ES cells that are standardized with respect to design and genetic background that researchers can access through repositories," says Dr Haydn Prosser, lead author on the research from the Wellcome Trust Sanger Institute. "In many cases, microRNAs with overlapping messenger RNA target specificities occur at multiple locations throughout the genome. To address these complexities a comprehensive resource is valuable to enable the creation of compound mutants in cells or mice."

The first step was to develop DNA vectors to target the microRNA genes: the team produced two alternative vectors that could, if desired, be used to knock out both copies of a particular microRNA
'/>"/>

Contact: Don Powell
press.office@sanger.ac.uk
44-012-234-96928
Wellcome Trust Sanger Institute
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. SIB launches next generation ExPASy, an entry portal to indispensable bioinformatics resources
2. Madagascar marine resources plundered by international seafood markets
3. New project will collect vital knowledge about tree genetic resources to support conservation
4. Surprise! Biodiversity and resource use may co-exist in tropical forests
5. Recycling perlite: New, improved method saves resources
6. Elsevier and the Arabidopsis Information Resource connect research articles
7. 3-D digital dinosaur track download: A roadmap for saving at-risk natural history resources
8. Restructuring natural resource majors
9. New user-friendly resource connects human genes to biological functions
10. American Chemical Society posts new online collection of resources on climate change
11. Resource restoration planning process begins for BP/Deepwater Horizon oil spill
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:8/28/2014)...  Privacy Advocate and Senior Staff Attorney at the ... the lineup of biometric and mobile commerce experts set ... Summit in Tampa, Florida on ... include Steven Rahman, Director, Technology and Strategy at Samsung, and ... theme of this year,s event is Mobility at the ...
(Date:8/28/2014)... WASHINGTON, Aug. 28, 2014A new method for measuring and ... help doctors and researchers better understand how drug abuse ... surgery and tissue engineering, and lead to better treatment ... by a team of researchers from Stony Brook University ... of Health, was published today in The Optical Society,s ...
(Date:8/28/2014)... mould in homes could pose a significant health risk to ... the Journal of Allergy and Clinical Immunology . , ... different countries, the research has found that the presence of ... asthma sufferers, as well as increasing the likelihood of developing ... team at the University of Exeter Medical School and is ...
Breaking Biology News(10 mins):Biometrics UnPlugged Welcomes the Electronic Frontier Foundation's Jennifer Lynch to the Mobility at the Crossroads of Commerce and Privacy Summit 2This is your brain's blood vessels on drugs 2This is your brain's blood vessels on drugs 3Indoor mold poses health risk to asthma sufferers 2
... still images and video, as well as local information ... of Life via new tools that let the public ... collaboration of unprecedented scale. Experts and citizen scientists ... which dedicates a Web page to each known species ...
... , , ... LLC, the image analysis and pattern recognition technology provider, today ... for online signature verification at the tenth International Conference on ... 26 - 29, 2009. Parascript was among 12 participants ...
... An indigenous family living in a mountainous area of ... discover information about genetic mutations associated with acromegaly, a form ... facial features. The information could lead to ... a benign pituitary gland tumor that can be deadly if ...
Cached Biology News:Cool new tools let public contribute to massive interactive online biodiversity encyclopedia 2Cool new tools let public contribute to massive interactive online biodiversity encyclopedia 3Cool new tools let public contribute to massive interactive online biodiversity encyclopedia 4Cool new tools let public contribute to massive interactive online biodiversity encyclopedia 5Cool new tools let public contribute to massive interactive online biodiversity encyclopedia 6Cool new tools let public contribute to massive interactive online biodiversity encyclopedia 7Parascript Technology Wins Online Signature Verification Competition 2Clues to gigantism provided by family in Borneo Mountains 2
(Date:8/28/2014)... PrimeSource Building Products, Inc., a leading ... to support their communication needs with the ... conferencing platform GlobalMeet powered by SoundConnect. The solution ... SoundConnect delivers award winning “results driven” business class ... be utilizing GlobalMeet powered by SoundConnect ...
(Date:8/28/2014)... Whitehouse Laboratories is excited to announce ... PTI Inspection Systems that will enable it ... art leak testing method development and validation programs using ... As part of this agreement, Whitehouse Labs has taken ... Instrument developed and manufactured by PTI. The HVLD ...
(Date:8/27/2014)... Austin, TX (PRWEB) August 27, 2014 ... $6 million in Series B funding and secured Otter ... and investment capital will accelerate commercialization of GGI’s Agriplier™ ... multiple crop types. , “Since our first meeting, ... commitment to the biological product space,” said Alan Sobba, ...
(Date:8/27/2014)... Livermore, California (PRWEB) August 27, 2014 ... (LLNL), through Lawrence Livermore National Security ... million to develop and deliver a state-of-the-art laser ... Beamlines facility (ELI Beamlines), under construction in the ... design performance, the laser system, called the " ...
Breaking Biology Technology:PrimeSource Chooses SoundConnect as Collaboration Provider 2Whitehouse Labs Renews Partnership with PTI Inspection Systems 2Whitehouse Labs Renews Partnership with PTI Inspection Systems 3Green & Grow Inc. Secures $6M Series B Funding from Otter Capital 2Green & Grow Inc. Secures $6M Series B Funding from Otter Capital 3LLNL Synchs up with ELI Beamlines on Timing System 2LLNL Synchs up with ELI Beamlines on Timing System 3
... Biomedical, Inc., a,leader in human embryonic stem cell ... an exclusive worldwide technology licensing agreement,with the University ... to commercialize human mesenchymal cells developed at the,University ... Under the terms of the agreement, ArunA ...
... ,endometrial regenerative cells, (ERCs) are capable of restoring ... peripheral artery disease. A study published today in ... demonstrates that when circulation-blocked mice were treated with ... , Critical limb ischemia, an advanced form ...
... Builds on Existing Brand Strength and Pediatric ... ... PLYMOUTH MEETING, Pa., Aug. 18 /Xinhua-PRNewswire-FirstCall/ -- BMP,Sunstone Corporation ... (Tangshan) Pharmaceutical Co., Ltd.,("Sunstone"), its wholly-owned subsidiary, has received a ...
Cached Biology Technology:ArunA Biomedical Announces Exclusive Worldwide Technology Licensing Agreement to Commercialize Human Embryonic Stem Cell Derived Mesenchymal Cells 2Limbs saved by menstrual blood stem cells 2BMP Sunstone Receives Production License from the SFDA to Manufacture and Market Pediatric Multivitamin Granules in the PRC 2BMP Sunstone Receives Production License from the SFDA to Manufacture and Market Pediatric Multivitamin Granules in the PRC 3BMP Sunstone Receives Production License from the SFDA to Manufacture and Market Pediatric Multivitamin Granules in the PRC 4
... cultured in RPMI 1640 medium with 2 mM ... growth. In order to keep the antigens ... acetone-methanol. The cells are arrayed on a ... wells surface specifically treated to enhance cellular attachment ...
Mouse polyclonal antibody to REXO4 - XPMC2 prevents mitotic catastrophe 2 homolog (Xenopus laevis)...
... StabilCoat Plus Microsphere/Microarray Stabilizer is an aqueous ... and other non-toxic chemicals in a PBS ... 7.4. This product contains a combination ... a preservative. StabilCoat Plus Stabilizer provides ...
... ABgene's Protein Purification Plates are designed ... SDS-PAGE gels. The 96-well plates feature two ... ends of each well. The wells remain ... with buffer, during which time the protein ...
Biology Products: