Navigation Links
New probes from Scripps research quantify folded and misfolded protein levels in cells
Date:3/4/2014

udy, however, the researchers used folding probes in combination with cell lysis and ATP depletion, which causes the chaperones in the cell to hold onto the unfolded proteomepreventing its folding, providing a snapshot of the folded protein-of-interest population, while minimizing overrepresentation of that state by the tagging process.

One of the most important applications of new probes like these will be for the rapid, "high-throughput" screening of very large drug compound libraries to identify drug candidates that prevent protein misfolding by improving the quality of cellular folding. "Using these probes to quantify the concentration of a functional, folded protein-of-interest, we can screen for compounds that boost this concentration, for example," said Zhang, who along with Kelly conceived and designed the study.

In this study, the researchers cleared another hurdle for the use of probes in high-throughput screens with the design of a probe whose fluorescent beacon isn't lit all the time, but only turns on when it reacts with the folded protein-of-interest. "That fluorescent signal quickly shows you the concentration of the folded, functional protein that was in the cell at the time of lysis," Zhang said. "There is no need for the time-consuming removal of fluorescence probes that aren't bound to targets or separation of the probeprotein-of-interest conjugate."

Drugs that reduce the misfolding of specific proteins by altering cell biology of protein homeostasis might one day be used to prevent or delay age-related neurodegenerative diseases, such as Alzheimer's and Parkinson's, and to treat inherited enzyme-deficiency disorders. Zhang, Kelly and their colleagues suspect that anti-misfolding drugs could have even broader applications, given the surprisingly large populations of misfolded proteins in cells and all the ways in which those misfolded proteins could be causing harm.


'/>"/>

Contact: Mika Ono
mikaono@scripps.edu
858-784-2052
Scripps Research Institute
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. Light and nanoprobes detect early signs of infection
2. Mercyhurst University study probes impact of climate change on ectotherms
3. Tiny probes shine brightly to reveal the location of targeted tissues
4. Rice University researchers optimize photoluminescent probes to study DNA and more
5. Not-so-precious: Stripping gold from AFM probes allows better measurement of picoscale forces
6. The shape of things to come: NIST probes the promise of nanomanufacturing using DNA origami
7. Scripps scientist awarded $1.8 million to develop new approaches to lung cancer therapy
8. Scripps Florida scientists offer new insight into neuron changes brought about by aging
9. Scripps leads first global snapshot of key coral reef fishes
10. Scripps Research Institute scientists achieve most detailed picture ever of key part of hepatitis C
11. Scripps oceanography researchers engineer breakthrough for biofuel production
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
New probes from Scripps research quantify folded and misfolded protein levels in cells
(Date:4/14/2015)... , April 14, 2015  HYPR Corp. today ... (FIDO ® ) Alliance tm , an industry ... members commit to share technology and collaborate to ... are interoperable, more secure and private, and easier ... identity verification that protects sensitive user information and ...
(Date:4/9/2015)... , April 9, 2015 Synaptics Inc. (NASDAQ: ... announced today that it will report financial results for ... 23, 2015, after the close of market. The company ... investors at 2:00 p.m. PT (5:00 p.m. ET), during ... To participate on the live call, analysts and investors ...
(Date:4/2/2015)... Sweden , April 2, 2015 ... for Q1 2015 attributed to somewhat higher than 125 ... in the year-end report 2014 that revenues for Q1 ... that the company reported for Q4 2014. The operating ... to be negative. The complete interim report will as ...
Breaking Biology News(10 mins):HYPR Corp. Joins the FIDO Alliance 2HYPR Corp. Joins the FIDO Alliance 3Synaptics to Report Third Quarter Results on April 23 2Fingerprint Cards Revises Revenues for Q1 2015 2
... are hard to beat. They can adhere to virtually ... in saltwater, including turbulent tidal environments. Little is known, ... feat. , In a paper to be published online ... the National Academy of Sciences, a Northwestern University research ...
... two more cardiac genes to the list of potential ... possibility that genetic defects of the heart may cause ... will be presented Friday at Heart Rhythm 2006, the ... in Boston. , In the two recent separate studies, ...
... up while hibernating may be an evolutionary mechanism to fight ... could offer an insight into the spread and emergence of ... health. , Many warm-blooded animals slip into an ... get past harsh winters when food supplies are low and ...
Cached Biology News:Study reveals details of mussels' tenacious bonds 2Researchers link two more genes to sudden infant death syndrome 2Breaks in hibernation help fight bugs 2
(Date:5/5/2015)... DUBLIN , May 05, 2015 /PRNewswire/ ... ) has announced the addition of the ... report to their offering. Macromolecules ... acids undergo separation or are broken into ... biotechnological products. This separation requires various advanced ...
(Date:5/5/2015)... 5, 2015 Reprints Desk, Inc., a Research ... access to scientific, technical and medical (STM) content, today ... Association of Southeastern Research Libraries (ASERL) . ... across 11 southeastern states. Founded in 1956, ASERL has ... to address the continually changing needs of its member ...
(Date:5/5/2015)... Research and Markets ( http://www.researchandmarkets.com/research/x5gjj3/clinical ... "Clinical Laboratory, Molecular Diagnostics and Genomic Testing Services ... Global Version" report to their offering. ... of molecular diagnostics and pharmacogenics hold the promise ... that is moving out of the national and ...
(Date:5/5/2015)... 05, 2015 Exotic Automation ... announces the launch of http://www.cng.exoticautomation.com , an ... a convenient way to purchase genuine OEM replacement ... , The new website provides owners of ... direct access to high performance products for their ...
Breaking Biology Technology:Global Separation Systems Market in Biotechnology 2015-2019 - Trends and Forecasts for the $28 Billion Market 2Association of Southeastern Research Libraries Partners with Reprints Desk to Improve Access for Hard-to-Find Materials 2Association of Southeastern Research Libraries Partners with Reprints Desk to Improve Access for Hard-to-Find Materials 3Association of Southeastern Research Libraries Partners with Reprints Desk to Improve Access for Hard-to-Find Materials 4Global Clinical Laboratory, Molecular Diagnostics and Genomic Testing Services Market Trends and Forecast 2015 to 2019 2Exotic Automation & Supply Launches Ecommerce Website 2
... growth rose slightly, with 17,000 more jobs created in April. Though there ... overall is off by about 40 percent compared to 2008. , ... ... in healthcare off by about 40 percent from first quarter 2008, job ...
... Germany, the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) ... has just been approved by the responsible Grants ... Research Training Groups offer doctoral researchers the opportunity ... and qualification programme at a high level of ...
... ) last week analyzed the most recent peptide ... infectivity of the H1N1 virus will increase markedly, ... the immediate future. The company,s quantitative analysis of ... a standard scientific repository for published papers, showed ...
Cached Biology Technology:The MedZilla Report: April 2009 Employment Outlook for Biotech/Pharma/Health 2The MedZilla Report: April 2009 Employment Outlook for Biotech/Pharma/Health 3DFG establishes 14 new research training groups 2DFG establishes 14 new research training groups 3DFG establishes 14 new research training groups 4DFG establishes 14 new research training groups 5DFG establishes 14 new research training groups 6DFG establishes 14 new research training groups 7Swine Flu (H1N1) Infectivity to Increase Markedly and Lethality to Remain Low According to Latest Replikin* Peptide Genomic Data 2Swine Flu (H1N1) Infectivity to Increase Markedly and Lethality to Remain Low According to Latest Replikin* Peptide Genomic Data 3
... analysis of gene expression) technology ... Expression profiles generated by SAGE ... low-abundance transcripts making it possible ... I-SAGE Kit is designed to ...
Incomplete Adjuvant...
2,4-Dinitrophenyl hapten is conjugated to Keyhole Limpet (KLH) protein through lysine....
...
Biology Products: