Navigation Links
New probes from Scripps research quantify folded and misfolded protein levels in cells
Date:3/4/2014

udy, however, the researchers used folding probes in combination with cell lysis and ATP depletion, which causes the chaperones in the cell to hold onto the unfolded proteomepreventing its folding, providing a snapshot of the folded protein-of-interest population, while minimizing overrepresentation of that state by the tagging process.

One of the most important applications of new probes like these will be for the rapid, "high-throughput" screening of very large drug compound libraries to identify drug candidates that prevent protein misfolding by improving the quality of cellular folding. "Using these probes to quantify the concentration of a functional, folded protein-of-interest, we can screen for compounds that boost this concentration, for example," said Zhang, who along with Kelly conceived and designed the study.

In this study, the researchers cleared another hurdle for the use of probes in high-throughput screens with the design of a probe whose fluorescent beacon isn't lit all the time, but only turns on when it reacts with the folded protein-of-interest. "That fluorescent signal quickly shows you the concentration of the folded, functional protein that was in the cell at the time of lysis," Zhang said. "There is no need for the time-consuming removal of fluorescence probes that aren't bound to targets or separation of the probeprotein-of-interest conjugate."

Drugs that reduce the misfolding of specific proteins by altering cell biology of protein homeostasis might one day be used to prevent or delay age-related neurodegenerative diseases, such as Alzheimer's and Parkinson's, and to treat inherited enzyme-deficiency disorders. Zhang, Kelly and their colleagues suspect that anti-misfolding drugs could have even broader applications, given the surprisingly large populations of misfolded proteins in cells and all the ways in which those misfolded proteins could be causing harm.


'/>"/>

Contact: Mika Ono
mikaono@scripps.edu
858-784-2052
Scripps Research Institute
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. Light and nanoprobes detect early signs of infection
2. Mercyhurst University study probes impact of climate change on ectotherms
3. Tiny probes shine brightly to reveal the location of targeted tissues
4. Rice University researchers optimize photoluminescent probes to study DNA and more
5. Not-so-precious: Stripping gold from AFM probes allows better measurement of picoscale forces
6. The shape of things to come: NIST probes the promise of nanomanufacturing using DNA origami
7. Scripps scientist awarded $1.8 million to develop new approaches to lung cancer therapy
8. Scripps Florida scientists offer new insight into neuron changes brought about by aging
9. Scripps leads first global snapshot of key coral reef fishes
10. Scripps Research Institute scientists achieve most detailed picture ever of key part of hepatitis C
11. Scripps oceanography researchers engineer breakthrough for biofuel production
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
New probes from Scripps research quantify folded and misfolded protein levels in cells
(Date:8/24/2015)... South Africa , August 24, 2015 ... Germany ,s largest biometrics manufacturer DERMALOG and its ... with the "2015 African Biometrics Company of the Year Award". ... Nigeria .   -Cross reference: ... http://www.presseportal.de/nr/8896 - On Thursday evening, in ...
(Date:8/20/2015)... -- NXT-ID, Inc. (NASDAQ: NXTD ) ("NXT-ID" or ... growing mobile commerce market and creator of the Wocket® ... Alliance and the EMV Migration Forum.  ... Alliance Latin America (SCALA) Chapter provides NXT-ID, Inc. direct ... promoting adoption of smart card technology. To ...
(Date:8/17/2015)... 2015  NXT-ID, Inc. (NASDAQ: NXTD ) ("NXT-ID" ... the growing mobile commerce market and creator of the ... Q4 2015. The new marketing campaign is being spearheaded ... Marketing Group ( http://amplitudemarketing.com/ ) and TPMG ( http://tmpg.com/ ... in retail driven marketing and brand awareness and has ...
Breaking Biology News(10 mins):Frost & Sullivan Honors DERMALOG with Biometrics Prize for Achievements in Africa 2Frost & Sullivan Honors DERMALOG with Biometrics Prize for Achievements in Africa 3NXT-ID, Inc. Joins Smart Card Alliance and EMV Migration Forum 2NXT-ID, Inc. Joins Smart Card Alliance and EMV Migration Forum 3NXT-ID, Inc. Joins Smart Card Alliance and EMV Migration Forum 4NXT-ID, Inc. Launches Sales Campaign for Wocket Smart Wallet with Award Winning Marketing Team 2NXT-ID, Inc. Launches Sales Campaign for Wocket Smart Wallet with Award Winning Marketing Team 3
... view is that DNA and genes control everything of importance ... thought. , Increasingly, however, scientists are realizing that among the ... from DNA, many interact with each other and with genes ... , In particular, RNA produced by the vast stretches ...
... known as SOS1 account for many cases of Noonan ... in one in 1,000-2,500 live births. NS is ... as well as heart problems and predisposition to leukemia. ... Medical School-Partners Healthcare Center for Genetics and Genomics (HPCGG) ...
... a detrimental effect on a person's ability to recover ... September Journal of Nuclear Medicine. , "Using positron emission ... specific model to study the regenerative capacity of the ... heart's ability to develop new nerves to replace damaged ...
Cached Biology News:Peering into the shadow world of RNA 2Peering into the shadow world of RNA 3Study uncovers mutation responsible for Noonan Syndrome 2Diabetes slows nerve recovery after heart transplant 2
(Date:8/27/2015)... ... August 27, 2015 , ... Inc. Magazine released its annual list ... as number 1,361 in growth for the three years through 2014. Being named to ... privately held organizations in the country. , “We are thrilled to make the ...
(Date:8/27/2015)... TRIANGLE PARK, N.C. , Aug. 27, 2015 ... on the decision by FedEx, UPS and The ... of certain biological specimens classified as ,select agents, ... and long history of handling these sensitive shipments. ... knowledge of global transportation regulations, trained and certified ...
(Date:8/26/2015)... (PRWEB) , ... August 26, 2015 , ... ... Solutions, announces today that a second US laboratory is to open in Manhattan, ... with the continued partnership and long-term growth of research and development through collaboration ...
(Date:8/26/2015)... -- Roka Bioscience, Inc. (NASDAQ: ROKA ), a molecular ... the detection of foodborne pathogens, today announced that Paul ... Company Emerging Growth Conference on September 2 at 9:20am ... York Marriott Marquis. A live webcast ... relations section of Roka Bioscience,s website at http://rokabio.investorroom.com/ ...
Breaking Biology Technology:iLab Solutions Named to 2015 Inc. 5000 List 2iLab Solutions Named to 2015 Inc. 5000 List 3Marken Ensures Safe Transportation Of Select Agents And Toxins 2MediVet Biologics Animal Health Secures Additional Laboratory Facility in Manhattan, Kansas 2MediVet Biologics Animal Health Secures Additional Laboratory Facility in Manhattan, Kansas 3
... Inc. (Nasdaq: PCYC ), a biopharmaceutical company focused ... treatment of immune mediated disease and cancer, today announced that ... 10, at 11:30 a.m. ET at the 8th Annual Needham ... Hotel. , , There will be a live webcast ...
... employees enjoy after work social and pledge drive , , ... Americas Restaurant on The Waterway in ... Oncology, Inc . delighted in an after work social and ... . , , (Photo: http://www.newscom.com/cgi-bin/prnh/20090605/DA28382 ) , ...
... is holding Platoon University at Bryn Mawr Outdoor Fitness Center, from ... MAWR, Pa., June 5 Platoon Fitness is gearing up for ... around the country in its outdoor training methods. , , ... in the realm of outdoor fitness. Over 1100 exercises will be ...
Cached Biology Technology:Pharmacyclics to Present at the 8th Annual Needham and Company Life Sciences Conference 2Americas on the Waterway Supports US Oncology Foundation 2Americas on the Waterway Supports US Oncology Foundation 3
... The Biometra TProfessional Thermocycler combines top ... silver block achieves high speed and unparalleled ... graphical display providing programming in a spreadsheet ... High Performance Smart Lid opens on the ...
...
... Kit is an advanced system for isolating DNA from ... be used directly for any downstream application. ... to use in 56 minutes Low melting point ... and TBE gels No specialised equipment required ...
... Columns provide a rapid and simple method for ... to 10 kb) from stained agarose gels or ... from an agarose gel and loaded into the ... column are a series of membranes and filters ...
Biology Products: