Navigation Links
New probes from Scripps research quantify folded and misfolded protein levels in cells
Date:3/4/2014

udy, however, the researchers used folding probes in combination with cell lysis and ATP depletion, which causes the chaperones in the cell to hold onto the unfolded proteomepreventing its folding, providing a snapshot of the folded protein-of-interest population, while minimizing overrepresentation of that state by the tagging process.

One of the most important applications of new probes like these will be for the rapid, "high-throughput" screening of very large drug compound libraries to identify drug candidates that prevent protein misfolding by improving the quality of cellular folding. "Using these probes to quantify the concentration of a functional, folded protein-of-interest, we can screen for compounds that boost this concentration, for example," said Zhang, who along with Kelly conceived and designed the study.

In this study, the researchers cleared another hurdle for the use of probes in high-throughput screens with the design of a probe whose fluorescent beacon isn't lit all the time, but only turns on when it reacts with the folded protein-of-interest. "That fluorescent signal quickly shows you the concentration of the folded, functional protein that was in the cell at the time of lysis," Zhang said. "There is no need for the time-consuming removal of fluorescence probes that aren't bound to targets or separation of the probeprotein-of-interest conjugate."

Drugs that reduce the misfolding of specific proteins by altering cell biology of protein homeostasis might one day be used to prevent or delay age-related neurodegenerative diseases, such as Alzheimer's and Parkinson's, and to treat inherited enzyme-deficiency disorders. Zhang, Kelly and their colleagues suspect that anti-misfolding drugs could have even broader applications, given the surprisingly large populations of misfolded proteins in cells and all the ways in which those misfolded proteins could be causing harm.


'/>"/>

Contact: Mika Ono
mikaono@scripps.edu
858-784-2052
Scripps Research Institute
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. Light and nanoprobes detect early signs of infection
2. Mercyhurst University study probes impact of climate change on ectotherms
3. Tiny probes shine brightly to reveal the location of targeted tissues
4. Rice University researchers optimize photoluminescent probes to study DNA and more
5. Not-so-precious: Stripping gold from AFM probes allows better measurement of picoscale forces
6. The shape of things to come: NIST probes the promise of nanomanufacturing using DNA origami
7. Scripps scientist awarded $1.8 million to develop new approaches to lung cancer therapy
8. Scripps Florida scientists offer new insight into neuron changes brought about by aging
9. Scripps leads first global snapshot of key coral reef fishes
10. Scripps Research Institute scientists achieve most detailed picture ever of key part of hepatitis C
11. Scripps oceanography researchers engineer breakthrough for biofuel production
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
New probes from Scripps research quantify folded and misfolded protein levels in cells
(Date:8/27/2014)... -- Duke University researchers have identified a gene that ... OSCA1, encodes a protein in the cell membrane of ... the plant,s water conservation machinery accordingly. , "It,s ... professor of biology at Duke. , The findings, ... , could make it easier to feed the world,s ...
(Date:8/27/2014)... neural circuits in the brain of mice, scientists ... The research, led by Howard Hughes Medical Institute ... Technology (MIT), reveals that the connections between the ... about an experience and the part of the ... experience are malleable. , Altering those connections ...
(Date:8/27/2014)... is entering what is described by the Volunteer Wildfire ... The Eastern Cape provincial government warned residents in certain ... winds and veld fires. A high veld fire ... along the coast in the Great Kei and Mnquma ... and during thunderstorms. The thunderstorms bring lightning strikes ...
Breaking Biology News(10 mins):Water 'thermostat' could help engineer drought-resistant crops 2Researchers change the emotional association of memories 2Researchers change the emotional association of memories 3Researchers change the emotional association of memories 4
... the Smiley lab at the Trudeau Institute have now identified ... be used as a vaccine. This single "subunit" could potentially ... cell-stimulating plague vaccine. The new data is featured in the ... "To date, there has been little progress in the development ...
... the next big innovation in sustainable climate technology? ... for Business Law and Regulation at Case Western Reserve ... do just that. "Technology-induced prizes have a long ... on a Climate Prize Rewarding Energy Innovation to Achieve ...
... Highlights: Advanced technologies create ... Radio was integrated easily with Army,s cellular biometric network ... Harris Corporation,s (NYSE: HRS ... infrastructure for various mission scenarios at the Empire Challenge 2011 ...
Cached Biology News:Trudeau Institute announces new discovery in battle against plague and bacterial pneumonias 2CWRU law professor eyes prize-based incentives to generate climate innovation 2Harris Corporation's Highband Networking Radio Provides Mobile, Wideband Communications for Empire Challenge 2011 2Harris Corporation's Highband Networking Radio Provides Mobile, Wideband Communications for Empire Challenge 2011 3
(Date:8/27/2014)... is how plants survive impact by the huge ... while using this energy for photosynthesis. The hypothesis ... blades quickly dissipate the energy throughout the entire ... DTU Physics have now managed to successfully ,film, ... contain light-absorbing proteins which play a role in ...
(Date:8/27/2014)... , Aug. 27, 2014  Lisa Kulik saw fireworks ... July 4 holiday, thanks to a groundbreaking retinal implant ... internationally renowned clinician-researcher at the University of Southern California ... Peoria, Ariz. resident who has ... victims of sight. On June 2, she became the ...
(Date:8/27/2014)... -- Reportbuyer.com has added a new market research ... Rational View https://www.reportbuyer.com/product/1595336/The-Graphene-Opportunity---A-Rational-View.html ... similar amount of hype currently surrounds graphene, with ... water treatment, but is graphene the only solution? ... Opportunity Report, authored by Tim Harper ...
(Date:8/27/2014)... 27, 2014  ARCH Venture Partners, one of ... the development of seed and early-stage advanced technology ... closed with more than $400 million in subscriptions. ... subscription target by more than $150 million. ... strength and potential of our approach to finding ...
Breaking Biology Technology:DTU researchers film protein quake for the first time 2USC Eye Institute ophthalmologists implant first FDA-approved Argus II retinal prosthesis in western United States 2USC Eye Institute ophthalmologists implant first FDA-approved Argus II retinal prosthesis in western United States 3USC Eye Institute ophthalmologists implant first FDA-approved Argus II retinal prosthesis in western United States 4USC Eye Institute ophthalmologists implant first FDA-approved Argus II retinal prosthesis in western United States 5The Graphene Opportunity - A Rational View 2The Graphene Opportunity - A Rational View 3The Graphene Opportunity - A Rational View 4Latest ARCH Venture Partners Fund Closes Above $400 Million 2Latest ARCH Venture Partners Fund Closes Above $400 Million 3
... educated nearly 80,000 patients in over 30 states on ... disease and treatment options since 1997, DEERFIELD, Ill., ... Baxter Healthcare Corporation,s Kidney Patient,Educator (KPE) program, an ambitious ... disease (CKD) and treatment options to,nearly 80,000 kidney disease ...
... at 11:45 am ET on November 8, 2007, ... (Nasdaq: TARG ) today announced its participation,in ... Conference, taking place November 8, 2007 at the,Hilton ... Officer of Targanta, will be presenting at 11:45 ...
... MNKD ) today announced results from a ... GLP-1 (glucagon-like peptide) on Technosphere,particles that is delivered ... as an open-label, dose escalation, controlled safety and,tolerability ... was assigned to one of five dose levels ...
Cached Biology Technology:Baxter Kidney Patient Education Program Celebrates Tenth Anniversary 2Baxter Kidney Patient Education Program Celebrates Tenth Anniversary 3Baxter Kidney Patient Education Program Celebrates Tenth Anniversary 4Targanta to Present at 9th Annual Mass Opportunities Investment Conference 2MannKind Announces Positive Results from a Phase 1a Study of a GLP-1 Formulation 2MannKind Announces Positive Results from a Phase 1a Study of a GLP-1 Formulation 3MannKind Announces Positive Results from a Phase 1a Study of a GLP-1 Formulation 4
... ViaSure Kit is used in conjunction with the ... cells automatically. The ViaSure Staining Reagent uses ... Acridine Orange and Ethidium Bromide to identify live ... while the EasyCount Slide 6 Multi-well Slide ...
... The Mini Trans-Blot cell and PowerPac Basic power ... to membranes. The Mini Trans-Blot cell accommodates two ... gel holder cassettes, modular electrode assembly, Bio-Ice cooling ... 4 fiber pads. The PowerPac basic power supply, ...
...
... SPE is a powerful, high-throughput workstation dedicated specifically for automating Solid Phase Extraction ... large volumes aqueous solution extractions (up to 2000ml's). ... ... ...
Biology Products: