Navigation Links
New paradigm for cell-specific gene delivery
Date:6/22/2008

Researchers from Northwestern University and Texas A & M University have discovered a new way to limit gene transfer and expression to specific tissues in animals. In studies to determine how plasmids enter the nuclei of non-dividing cells, the group previously identified a region of a smooth muscle cell-specific promoter that was able to mediate nuclear targeting of any plasmid carrying this sequence uniquely in cultured smooth muscle cells but in no other cell type. In their current study to appear in the July 08 issue of Experimental Biology and Medicine, the team, led by Drs. David Dean and Jennifer Young from the Department of Medicine at Northwestern University, in collaboration with Warren Zimmer from Texas A & M University, now demonstrate that such restriction of nuclear entry using this specific DNA sequence can be used in blood vessels of living animals to direct gene transfer and expression specifically to smooth muscle cells. They have also developed a novel gene delivery approach for the vasculature that uses an electric field to transiently permeabilize the plasma membrane of cells to allow entry of DNA. Thus, this work establishes the control of nuclear entry of gene therapy vectors as a novel approach to target genes and gene expression to desired cell types in the body.

Vascular smooth muscle proliferative diseases, including atherosclerosis and restenosis, are among the leading causes of morbidity and mortality in the US. Gene therapy may represent an important alternative for the treatment and prevention of these proliferative diseases of the vasculature. It can be highly cell-specific, mimic or restore normal in vivo function, and can be permanent or transient depending on vector design. Currently, a number of gene delivery systems for use on the arterial wall are being studied, but as yet their low efficiency in gene transfer and lack of cell-specific targeting and expression are major limitations. According to Dr. D
'/>"/>

Contact: Dr. David Dean
david_dean@urmc.rochester.edu
585-276-3933
Society for Experimental Biology and Medicine
Source:Eurekalert

Page: 1 2

Related biology news :

1. Lets talk -- new paradigms in the research of the biomolecular composition of water
2. Paradigm shift in Alzheimerss research: new treatments
3. Test of bacteria toxin delivery system could pave way for new antibiotic drugs
4. MIT: Stripes key to nanoparticle drug delivery
5. Micro-origami: USC folds up micrometer-scale voxels for drug delivery
6. Fewer babies born after Caesarean delivery
7. Remarks Prepared for Delivery By Attorney General Michael B. Mukasey At the Ministerial Press Conference
8. e-SMART Technologies Continues Delivery of Its New Super SMART Card in South Korea
9. Researchers develop better membranes for water treatment, drug delivery
10. Carnegie Mellon researchers to develop new drug delivery system
11. Computer solution to delivery problem
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/24/2014)... has been published in the Scientific Reports journal. ... Biology (a joint centre of CSIC and Pompeu Fabra University-UPF), ... decoded and we saw that it did not match with ... to sequence the complete genome and to make a functional ... belong to Louis XVI". , The functional genome analysis ...
(Date:4/24/2014)... representing the largest study of animal intelligence to-date finds that ... , Published this week in the journal Proceedings of ... of a long history of research aimed at understanding the ... things like make and use tools, read social cues, or ... now, most studies of animal intelligence have focused on only ...
(Date:4/24/2014)... WASHINGTON, April 24A droplet of clear liquid can bend ... well-known phenomenon, researchers have developed a new process to ... than a penny apiece. , Because they,re so ... of applications, including tools to detect diseases in the ... and microscopes for education in classrooms. , "What ...
Breaking Biology News(10 mins):The blood preserved in the pumpkin did not belong to Louis XVI 2Animals with bigger brains, broader diets have better self control 2Animals with bigger brains, broader diets have better self control 3Bake your own droplet lens 2Bake your own droplet lens 3
... An Arizona State University research team has developed a ... renewable biofuels. The team has programmed a photosynthetic ... their biofuel byproducts--easier and potentially less costly. ... harvesting the goodies and turning them into fuel," said ...
... diffraction is a step closer to fulfilling its ... biological samples. In the Proceedings of the ... in applying an approach to "lensless" X-ray microscopy ... produced the first images, using this technique, of ...
... For more than 400 years, scientists have studied ... how these creatures routinely repair injuries that would usually ... Now, fueled by a highly competitive National Institutes ... multi-institutional team of researchers associated with the University of ...
Cached Biology News:Self-destructing bacteria improve renewable biofuel production 2Self-destructing bacteria improve renewable biofuel production 3Researchers demonstrate nanoscale X-ray imaging of bacterial cells 2$2.4 million stimulus fuels effort to regenerate injured spinal cords 2$2.4 million stimulus fuels effort to regenerate injured spinal cords 3$2.4 million stimulus fuels effort to regenerate injured spinal cords 4
(Date:1/15/2014)... JOLIET, Ill. , Jan. 15, 2014  Bill Jacobs Automotive, ... , is teaming up with the Heartland Blood Center ... annual blood drive. The drive takes place Saturday, Jan. 18 ... a.m. - 2 p.m. (Photo: http://photos.prnewswire.com/prnh/20140115/MN46637 )  ...
(Date:1/15/2014)... 15, 2014 Look inside the new ... for the lab, from fluid handling to instruments to ... ship when you order. , Preferred Solutions features ... from the L/S® model for precise flow control and ...
(Date:1/15/2014)... ­ RedBrick Health , a fast-growing leader in ... that EmblemHealth , the New York ... providing the RedBrick Compass health assessment, combined with the ... its members. EmblemHealth is among the first health plans ...
(Date:1/15/2014)... January 15, 2014 More than 5 ... about 1 in 3 seniors will die with Alzheimer’s ... These jaw-dropping figures have shocked many Americans into looking ... help prevent these tragic age-related cognitive disorders. Jonathan Weisman, ...
Breaking Biology Technology:Join the Bill Jacobs Auto blood drive and receive a free oil change 2Cole-Parmer Begins 2014 with the Release of Preferred Solutions 2EmblemHealth Selects RedBrick Health to Power its Next-Generation Health Engagement and Behavior Change System 2EmblemHealth Selects RedBrick Health to Power its Next-Generation Health Engagement and Behavior Change System 3Biohack Pure Offers 5 Tips for Increasing Memory in 2014 2
... ARBOR, Mich., Sept. 13 MichBio, the ... its 2007/8 Michigan Life,Sciences Directory and Resources ... sciences companies. The guide includes editorial pages ... the University,Research Corridor, the Michigan Universities Commercialization ...
... Mo. and FREDERICK, Md., Sept. 13 /PRNewswire-USNewswire/,-- To ... services,Midwest Research Institute (MRI) announced today the launch ... -- a center staffed by an,internationally recognized team ... to provide a diverse range of specialized,laboratory consulting ...
... Multiplexing Technology Symposium Brings Together Thought Leaders,in Life ... Best Practices, OOSTERHOUT, Netherlands, Sept. 13 ... in multiplexed solutions,today announced the agenda for Planet ... symposium. The two-day event will be,held 2-3 October ...
Cached Biology Technology:Midwest Research Institute Launches Center for Biological Safety and Security (CBS2) 2Luminex Corporation Hosts the Fifth Annual Planet xMAP Europe 2007 Symposium 2Luminex Corporation Hosts the Fifth Annual Planet xMAP Europe 2007 Symposium 3
... Multipeptide Kit, designed for use with ... variety of amyloid peptides more straightforward ... Clinical neuroscience proteomics applications - Monitor ... Correlate beta-amyloid peptide levels with disease ...
... hybridization chamber assembly (1 each) Components ... chamber user guide plastic tweezersChambers are ... cleaned and reused. The chamber is designed ... kits and all custom oligo microarrays, in ...
... Formula variant: With the ... described by Hoagland and ... Equivalency: Formulated to contain ... powder per liter of ...
... For separation and fractionation of ... of nuclei from cytosolic proteins ... complexity, greatly facilitating study of ... nucleic acid binding proteins (e.g., ...
Biology Products: