Navigation Links
New palm-sized microarray technique grows 1,200 individual cultures of microbes
Date:6/24/2013

A new palm-sized microarray that holds 1,200 individual cultures of fungi or bacteria could enable faster, more efficient drug discovery, according to a study published in mBio, the online open-access journal of the American Society for Microbiology.

Scientists at the University of Texas at San Antonio and the U.S. Army Institute of Surgical Research at Fort Sam Houston have developed a microarray platform for culturing fungal biofilms, and validated one potential application of the technology to identify new drugs effective against Candida albicans biofilms. The nano-scale platform technology could one day be used for rapid drug discovery for treatment of any number of fungal or bacterial infections, according to the authors, or even as a rapid clinical test to identify antibiotic drugs that will be effective against a particular infection.

"Even though we have used the antifungal concept for development, it is a universal tool," says co-author Jose Lopez-Ribot of the University of Texas at San Antonio. "It opens a lot of possibilities as a new platform for microbial culture. Any time you need large numbers of cultures, this has a big advantage over other methods."

"The possibility exists to use this same technology for pretty much any other organism," he says.

Microbiology and medicine have become increasingly reliant on micro- and nano-scale technologies because of the increased speed and efficiency they can offer, but until now the cultivation of microorganisms has mostly been conducted on larger scales, in flasks and in trays called micro-titer plates. The microarray technology enables the user to rapidly compare hundreds or thousands of individual cultures of bacteria or fungi, a big benefit in the search for new drugs to treat infections. And like many nano-scale techniques, the nano-culture approach described in the mBio study is also automated, a feature that saves time, improves reproducibility, and prevents some types of user error.

To test the technique, the authors embedded cells of the opportunistic pathogen C. albicans in each of the 1,200 tiny dots of alginate on the surface of the microarray. Under the microscope, these nano-biofilms of C. albicans, each of which was only 30 nanoliters, exhibited the same growth habits and other outward characteristics as conventional, macroscopic biofilms, and achieved maximum metabolic activity within 12 hours. The tiny cultures were then treated with a wide range of candidate drugs from the National Cancer Institute library, or with different FDA-approved, off-patent antifungal drugs in combination with FK506, an immunosuppressant, for identifying individual or synergistic combinations of compounds effective against biofilm infections. Co-author Anand Ramasubramanian of the University of Texas at San Antonio says that the tests prove the utility of the technology in screening combinations of drugs.

"The antifungal screening results were similar to results in larger macroscale techniques. That gives us confidence that it could be used as a tool to replace existing techniques," says Ramasubramanian.

Going forward, Ramasubramanian says he and his colleagues are testing the microarrays with polymicrobial cultures - mixtures of fungi and bacteria - to see whether the technology can be used to explore treatments for mixed infections. They are also exploring clinical applications for the technique, testing patient samples against an array of drugs or combinations of drugs to develop tailored therapies.

Lopez-Ribot says their microarray technique is just the latest development in a decades-long trend toward the tiny in science. "Things are moving toward smaller scale, more powerful techniques. You don't need millions of cells for these assays like we used to - maybe a few cells will do."


'/>"/>

Contact: Jim Sliwa
jsliwa@asmusa.org
202-942-9297
American Society for Microbiology
Source:Eurekalert

Related biology news :

1. Markets for PCR, DNA Microarray, DNA Sequencing, Mass Spectrometry and Flow Cytometry to Exceed $50 billion by 2015
2. DNA Microarray 2013 Market Report: A Focus on Sales Growth
3. New synthetic biology technique boosts microbial production of diesel fuel
4. New genetic bar code technique establishes ability to derive DNA information from RNA
5. Early detection techniques offer hope for improved outcomes in lung cancer patients
6. Scientists develop new technique that could improve heart attack prediction
7. New rearing system may aid sterile insect technique against mosquitoes
8. Its a trap! New laboratory technique captures microRNA targets
9. New screening technique yields elusive compounds to block immune-regulating enzyme
10. New stem cell technique promises abundance of key heart cells
11. Evaluation of microscopy techniques may help scientists to better understand ancient plants
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/9/2016)... Elevay is currently known as ... for high net worth professionals seeking travel for work ... world, there is still no substitute for a face-to-face ... your deal with a firm handshake. This is why ... of citizenship via investment programs like those offered by ...
(Date:4/28/2016)... , April 28, 2016 First quarter ... (139.9), up 966% compared with the first quarter of 2015 ... totaled SEK 589.1 M (loss: 18.8) and the operating margin was ... (loss: 0.32) Cash flow from operations was SEK 249.9 ... 2016 revenue guidance is unchanged, SEK 7,000-8,500 M. The ...
(Date:4/19/2016)... DUBAI , UAE, April 20, 2016 ... can be implemented as a compact web-based "all-in-one" system ... in the biometric fingerprint reader or the door interface ... requirements of modern access control systems. The minimal dimensions ... the ID readers into the building installations offer considerable ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... 2016   EpiBiome , a precision microbiome engineering ... debt financing from Silicon Valley Bank (SVB). The financing ... advance its drug development efforts, as well as purchase ... "SVB has been an incredible strategic partner to us ... bank would provide," said Dr. Aeron Tynes Hammack ...
(Date:6/23/2016)... ... 23, 2016 , ... In a new case report published today in STEM ... who developed lymphedema after being treated for breast cancer benefitted from an injection of ... dealing with this debilitating, frequent side effect of cancer treatment. , Lymphedema ...
(Date:6/23/2016)... , June 23, 2016 ... Review, 2016;12(1):22-8 http://doi.org/10.17925/OHR.2016.12.01.22 Published ... the peer-reviewed journal from touchONCOLOGY, Andrew D ... cost of cancer care is placing an increasing ... of expensive biologic therapies. With the patents on ...
(Date:6/23/2016)... ... June 23, 2016 , ... Regulatory Compliance ... consulting, provides a free webinar on Performing Quality Investigations: Getting to ... 12pm CT at no charge. , Incomplete investigations are still a major concern ...
Breaking Biology Technology: