Navigation Links
New optical tweezers trap specimens just a few nanometers across

To grasp and move microscopic objects, such as bacteria and the components of living cells, scientists can harness the power of concentrated light to manipulate them without ever physically touching them.

Now, doctoral student Amr Saleh and Assistant Professor Jennifer Dionne, researchers at the Stanford School of Engineering, have designed an innovative light aperture that allows them to optically trap smaller objects than ever before potentially just a few atoms in size.

The process of optical trapping or optical tweezing, as it is often known involves sculpting a beam of light into a narrow point that produces a strong electromagnetic field. The beam attracts tiny objects and traps them in place, just like a pair of tweezers.

Unfortunately, there are natural limits to the technique. The process breaks down for objects significantly smaller than the wavelength of light. Therefore, optical tweezers cannot grasp super-small objects like individual proteins, which are only a couple of nanometers in diameter.

Saleh and Dionne have shown theoretically that light passed through their novel aperture would stably trap objects as small as 2 nanometers. The design was published in the journal Nano Letters, and Saleh is now building a working prototype of the microscopic device.

Agonies of scale

As a materials scientist, Jennifer Dionne imagined an optical tool that would help her precisely move molecular building blocks into new configurations. "Optical tweezers seemed like a really cool way of assembling new materials," she said. Dionne is the paper's senior author.

Unfortunately, existing optical tweezers are not adept at handling these tiny building blocks. "It's been a known for several decades that trapping nano-sized objects with light would be challenging," said Dionne.

The problem is inherent to the light beam itself. Optical trapping typically uses light in the visible spectrum (with wavelengths between 400 and 700 nanometers) so that scientist can actually see the specimen as they manipulate it.

Due to a physical constraint called the diffraction limit of light, the smallest space in which optical tweezing can trap a particle is approximately half the wavelength of the light beam. In the visible spectrum this would be about 200 nanometers half the shortest visible wavelength of 400 nanometers.

Thus, if the specimen in question is only 2 nanometers wide the size of a typical protein trapping it in a space of 200 nanometers allows only very loose control at best. Scale-wise, it is akin to guiding a minnow with 20-meter-wide fishing net.

Additionally, the optical force that light can exert on an object diminishes as the objects get smaller. "If you want to trap something very small, you need a tremendous amount of power, which will burn your specimen before you can trap it," Saleh said.

Some researchers get around this problem by attaching the specimen to a much larger object that can be dragged around with light. Dionne noted, however, that important molecules like insulin or glucose might behave quite differently when attached to giant anchors than they would on their own. To isolate and move a tiny object without frying it, the researchers needed a way around the limitations of conventional optical trapping.

The promise of plasmonics

Dionne says that the most promising method of moving tiny particles with light relies on plasmonics, a technology that takes advantage of the optical and electronic properties of metals. A strong conductor like silver or gold holds its electrons weakly, giving them freedom to move around near the metal's surface.

When light waves interact with these mobile electrons, they move in what Dionne describes as "a very well-defined, intricate dance," scattering and sculpting the light into electromagnetic waves called plasmon-polaritons. These oscillations have a very short wavelength compared to visible light, enabling them to trap small specimens more tightly.

Dionne and Saleh applied plasmonic principles to design a new aperture that focuses light more effectively. The aperture is structured much like the coaxial cables that transmit television signals, Saleh said. A nanoscale tube of silver is coated in a thin layer of silicon dioxide, and those two layers are wrapped in a second outer layer of silver. When light shines through the silicon dioxide ring, it creates plasmons at the interface where the silver and silicon dioxide meet. The plasmons travel along aperture and emerge on the other end as a powerful, concentrated beam of light.

The Stanford device is not the first plasmonic trap, but it promises to trap the smallest specimens recorded to date. Saleh and Dionne have theoretically shown that their design can trap particles as small as 2 nanometers. With further improvements, their design could even be used to optically trap molecules even smaller.

An optical multi-tool

As nanoscale tools go, this new optical trap would be quite a versatile gadget. While the researchers first envisioned it in the context of materials science, its potential applications span many other fields including biology, pharmacology, and genomics.

Dionne said she would first like to trap a single protein, and try to unravel its twisted structure using visible light alone. Dionne points out that the beam of light could also be used to exert a strong pulling force on stem cells, which has been shown to change how the these important building blocks differentiate into various kinds of cells. Saleh, on the other hand, is particularly excited about moving and stacking tiny particles to explore their attractive forces and create new, "bottom-up" materials and devices.

All this is down the road, however. In the meantime, Saleh is working on turning the design into reality. He hopes to have a prototype by early 2013.


Contact: Andrew Myers
Stanford School of Engineering

Related biology news :

1. Optical waveguide connects semiconductor chips
2. Optical boomerangs, ultralight fractal materials, and more
3. Acoustic tweezers capture tiny creatures with ultrasound
4. Newly demonstrated capabilities of low-powered nanotweezers may benefit cellular-level studies
5. High-resolution atomic imaging of specimens in liquid by TEM using graphene liquid cell
6. TGen method isolates biospecimens for treatment of kidney disease
7. Disease-carrying colonizers on the move: Predicting the spread of ticks across Canada
8. Stoneflies mapped across Ohio, with implications for water quality and nature conservation
9. Researchers across North America team up to find genetic markers for autism
10. NASA satellites see wildfires across Colorado
11. Same adaptations evolve across different insects
Post Your Comments:
Related Image:
New optical tweezers trap specimens just a few nanometers across
(Date:11/17/2015)... 17, 2015  Vigilant Solutions announces today that Mr. ... of Directors. --> --> ... from the partnership at TPG Capital, one of the ... $140 Billion in revenue.  He founded and led TPG,s ... TPG companies, from 1997 to 2013.  In his first ...
(Date:11/16/2015)... JOSE, Calif. , Nov 16, 2015 /PRNewswire/ ... developer of human interface solutions, today announced expansion ... Synaptics TouchView ™ touch controller and display ... architectural revolution of smartphones. These new TDDI products ... include TD4100 (HD resolution), TD4302 (WQHD resolution), and ...
(Date:11/12/2015)...  Arxspan has entered into an agreement with ... use of its ArxLab cloud-based suite of biological ... will support the institute,s efforts to electronically manage ... internally and with external collaborators. The ArxLab suite ... Institute,s electronic laboratory notebook, compound and assay registration, ...
Breaking Biology News(10 mins):
(Date:11/24/2015)... 2015 Capricor Therapeutics, Inc. (NASDAQ: ... discovery, development and commercialization of first-in-class therapeutics, today announced ... is scheduled to present at the 2015 Piper Jaffray ... EST, at The Lotte New York Palace Hotel in ... . --> . ...
(Date:11/24/2015)... Nov. 24, 2015  PDL BioPharma, Inc. (PDL) (NASDAQ: ... the company,s president and chief executive officer, will present at ... week in New York City . The ... December 1, 2015 at 9:30 a.m. EST. ... the website at least 15 minutes prior to the presentation ...
(Date:11/24/2015)... ... November 24, 2015 , ... Whitehouse Laboratories is pleased to announce that ... facility will be strictly dedicated to basic USP 61, USP 62 and USP 51 ... to have complete chemistry and micro testing performed by one supplier. Management ...
(Date:11/23/2015)... with a certain type of lung nodule visible on lung ... cancer than men with similar nodules, according to a new ... the Radiological Society of North America ... Lung nodules are small masses of tissue in the lungs ... appearance on CT. Solid nodules are dense, and they obscure ...
Breaking Biology Technology: