Navigation Links
New microscopes at NIH reveal live, developing cells in unprecedented 3-D clarity
Date:10/25/2013

to light even though they are only imaging one small section at a time. However, just as the sun can damage skin cells, too much light exposure can damage or even kill biological samples like embryos. SPIM uses a thin beam of light to illuminate only the single plane that is currently being imaged so the biological sample is not damaged by overexposure to light. However, the technology is limited because looking at a 3-D object from only one point of view does not provide a complete representation of the object -- in the same way that viewing a globe from one perspective gives no information about what is on the other side of the world. Traditionally, SPIM microscopes rotate the sample so that they can clearly see all the dimensions, but this severely limits the imaging speed and can increase the damage done to the cells from light exposure because of the many extra images taken at multiple angles. As a result imaging is also slowed down, and the ability to capture much of the fast moving cellular motion is lost.

In order to combat this problem, Shroff and NIBIB staff scientist Yicong, Wu, Ph.D., developed a dual-view SPIM (diSPIM) microscope with two separate detection cameras. The cameras are set at a 90 degree angle to capture perpendicular views of the sample. This perpendicular view results in undistorted 3-dimensional images, and since only two views are acquired, the microscope can still capture events at very high speed. Additionally, with relatively simple modifications, traditional single camera SPIM microscopes can be converted into the dual-camera diSPIM. The real challenge in developing this technology was to find a way to combine the two disparate images from the two cameras, which required the creation of a new post-processing software algorithm.

The increased speed at which the new dual microscope can image the cells allows for clearer images of even very fast moving viruses. Being able to see how a virus enters a cell, and once
'/>"/>

Contact: Jessica Meade
nibibpress@mail.nih.gov
301-496-3500
NIH/National Institute of Biomedical Imaging & Bioengineering
Source:Eurekalert  

Page: 1 2 3 4

Related biology news :

1. Research reveals first evidence of hunting by prehistoric Ohioans
2. Studies reveal structure of EV71, a virus causing childhood illnesses
3. Researchers reveal how a single gene mutation leads to uncontrolled obesity
4. Study reveals how monarch butterflies recolonize northern breeding range
5. Circadian rhythms have profound influence on metabolic output, UCI study reveals
6. Unexpected discovery reveals a new mechanism for how the cerebellum extracts signal from noise
7. Ancient civilizations reveal ways to manage fisheries for sustainability
8. Study by Haverford College professor reveals unprecedented impact of Deepwater Horizon on deep ocean
9. Nature: Video reveals wave character of particles
10. Plant research reveals new role for gene silencing protein
11. UGA scientists reveal genetic mutation depicted in van Goghs sunflower paintings
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
New microscopes at NIH reveal live, developing cells in unprecedented 3-D clarity
(Date:4/17/2014)... to produce hydrogen sulfide in order to properly multiply ... from the Center for Craniofacial Molecular Biology at the ... Songtao Shi, principal investigator on the project, said the ... the flow of calcium ions. The essential ions activate ... or the creation of new bone tissue, and keeps ...
(Date:4/17/2014)... release is available in German . ... pay the protection money demanded of him, he can expect ... required, however, as fear of the consequences is enough to ... in parasitic birds, which lay their eggs in other birds, ... the brood parasites take their revenge by destroying the entire ...
(Date:4/17/2014)... have hit on a novel method to help ... and most effective treatment possible., Kidney stones represent ... developing world. If left untreated, apart from being ... and other complications. In many patients treated successfully, ... more effective pathological approach to diagnosis and treatment ...
Breaking Biology News(10 mins):Fear of the cuckoo mafia 2Fear of the cuckoo mafia 3
... 2004, Washington University in St. Lous researcher Jianmin Cui was ... channel his lab had been studying for five years. ... a large family whose members suffered from epilepsy, sudden attacks ... and paroxysmal dyskinesia (GEPD). By analyzing the DNA of ...
... attacks in the United States are fostering development of ... to protect people against the potentially deadly bacteria in ... sweeping overview of scientific research on medical technology to ... Journal of Medicinal Chemistry . In ...
... are reporting that particle size affects the toxicity of zinc ... 100 nanometers are slightly more toxic to colon cells than ... equivalent amounts of soluble zinc, and direct particle to cell ... in ACS, Chemical Research in Toxicology , a monthly ...
Cached Biology News:Solving the puzzle of the BK ion channel 2Solving the puzzle of the BK ion channel 3Solving the puzzle of the BK ion channel 4Solving the puzzle of the BK ion channel 5New medical weapons to protect against anthrax attacks 2
(Date:1/15/2014)... January 15, 2014 More than 5 ... about 1 in 3 seniors will die with Alzheimer’s ... These jaw-dropping figures have shocked many Americans into looking ... help prevent these tragic age-related cognitive disorders. Jonathan Weisman, ...
(Date:1/15/2014)... 2013 was a banner year of continued innovation ... continued independent research led by the team at Wake ... million grant from the Susanne Marcus Collins Foundation, had ... reviewed journal, Amy Grant highlighted Brainwave Optimization® in First ...
(Date:1/14/2014)... 2014 Date: Friday, April 11, 2014 , ... Club, 1360 Almshouse Road, Warrington, Pa. , Details: ... dedicated to finding a cure for hepatitis B and improving ... its annual Crystal Ball on Friday, April 11 at Warrington ...
(Date:1/14/2014)... 14, 2014 As pet owners drew up their lists of ... wrote: "Take better care of my furry companion." Nowadays there are ... top-brand carrying cases to take the little canine or feline darlings ... compelled to buy some pricey toys at the pet store. But ...
Breaking Biology Technology:Biohack Pure Offers 5 Tips for Increasing Memory in 2014 2Dynamic Innovative Technology Showcased at Scottsdale Company’s Open House 2Dynamic Innovative Technology Showcased at Scottsdale Company’s Open House 3Hepatitis B Foundation to Host Annual Crystal Ball Gala 2New Year's Resolution: Give Pets the Gift of Top-Notch Health Care 2
... movie about giant robots that undergo structural ... a scientific study about structural transformations within single ... of novel materials that will serve next-generation energy ... at the U.S. Department of Energy (DOE),s Lawrence ...
... 8, 2011 Northwest Biotherapeutics (OTC Bulletin Board: ... equity investment on June 28, 2011, by The Richard ... newly issued common stock of the Company to the ... 31, 2011, by investment funds managed by Whitebox Advisors, ...
... 7, 2011 For the first time since stem ... isolated a human blood stem cell in its purest ... regenerating the entire blood system. This breakthrough opens the ... to treat cancer and other debilitating diseases more effectively. ...
Cached Biology Technology:Nanocrystal transformers 2Nanocrystal transformers 3Nanocrystal transformers 4Family Foundation of Business Leader Invests $4.55 Million in Northwest Biotherapeutics for Promising Clinical Trials in the US and Europe 2Family Foundation of Business Leader Invests $4.55 Million in Northwest Biotherapeutics for Promising Clinical Trials in the US and Europe 3Family Foundation of Business Leader Invests $4.55 Million in Northwest Biotherapeutics for Promising Clinical Trials in the US and Europe 4"Pure" Human Blood Stem-Cell Discovery Opens Door to Expanding Cells for More Clinical Use 2"Pure" Human Blood Stem-Cell Discovery Opens Door to Expanding Cells for More Clinical Use 3
MpV17 transgene, murine homolog, glomerulosclerosis...
Mouse monoclonal [6C3] to JAB1 ( Abpromise for all tested applications). entrezGeneID: 10987 SwissProtID: O15386...
Recombinant Ovine (Sheep) Prolactin, Ultra Pure...
Human LT beta R/TNFRSF3 Phycoerythrin MAb (Clone 71319)...
Biology Products: