Navigation Links
New method to diagnose sepsis is faster, cheaper
Date:11/18/2013

identification methods are also available for diagnosis, but they can take at least a day or two to produce results and many require expensive equipment.

In the technique developed by Walsh et al., a sample of positive blood culture is treated with lysis buffer to "pop" the blood cells, then transferred to a specialized optical tube. The tube is centrifuged, which drives bacteria or fungi, which are denser than the solution, down through a liquid density cushion to form a pellet at the bottom of the tube.

Then comes the intrinsic fluorescence spectroscopy (IFS): the microbial pellet is irradiated with light ranging from the deep ultraviolet to infrared, which excites certain organic molecules in the microorganisms, including tryptophan, NADH, FAD, porphyrins, and others, and causes them to fluoresce in a characteristic way depending on the identity of the microbe. The exact pattern of fluorescence is compared with a database of 37 of the most common known pathogens to identify the organism present.

"We're using intrinsic fluorescence to identify the microorganisms. It's an innate property of most living organisms. Because it's intrinsic, no reagents are needed for the identification step," which removes many of the opportunities for mistakes and lowers test costs, says Walsh.

Testing in a controlled laboratory study shows the method can correctly identify the species in 96.5% of all test samples, and in the 2.7% of samples for which no species identity was provided, the system was able to correctly identify 67% to the family level, which is often enough information to select an effective therapy. Among over a thousand samples tested, the method never gave an incorrect result as to the family level or the Gram type.

Walsh says the research and development team in Durham is actively working on automating the system with robotics to make it a fully hands-off process. Blood cultures grow in their own time, often producing a positive result at an
'/>"/>

Contact: Garth Hogan
ghogan@asmusa.org
American Society for Microbiology
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. Rice University method gives accurate picture of gas storage by microscopic cages
2. MU study finds more accurate method to diagnose pancreatic cancer
3. New methods improve quagga and zebra mussel identification
4. Laser technology sorting method can improve Capsicum pepper seed quality
5. Researchers develop rapid, cost-effective early detection method for organ transplant injury
6. Scientists develop method that ensures safe research on deadly flu viruses
7. UTSA, Southwest Research Institute to develop low-cost method to treat fracking water
8. New methods to visualize bacterial cell-to-cell communication
9. Low-cost sterilization method for cats and dogs is focus of new research project
10. Memorial Sloan-Kettering researchers develop new method for tracking cell signaling
11. Researchers create method to rapidly identify specific strains of illness
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
New method to diagnose sepsis is faster, cheaper
(Date:9/18/2014)... September 18, 2014 New research into the Crimean-Congo ... a severe hemorrhagic disease in humans similar to that ... for CCHFV infection. This discovery has the potential to ... pathogen. , The research, reported in a ... and conducted by scientists at the Texas Biomedical ...
(Date:9/18/2014)... International Space Station ever starts a "frequent flyers" program, ... Recently the orbiting laboratory has hosted increasing numbers of ... from the space station in April, and another is ... experiment is planned to launch in December. , Fruit ... the world-- and in space. Model organisms can reveal ...
(Date:9/18/2014)... spoken language is unique to humans. "The genetic changes ... human evolution to make this possible are largely unknown, ... have," says Wolfgang Enard, Professor of Anthropology and Human ... molecular biological basis of language Enard has now taken ... study, undertaken in collaboration with scientists at several universities, ...
Breaking Biology News(10 mins):Research milestone in CCHF virus could help identify new treatments 2Buzzing with activity: Fruit flies orbit Earth for science 2Buzzing with activity: Fruit flies orbit Earth for science 3Buzzing with activity: Fruit flies orbit Earth for science 4Language evolution: Quicker on the uptake 2Language evolution: Quicker on the uptake 3
... 2010 IDenta Corp.,(PINKSHEETS: IDTA) CEO Yaacov Shoham today issued a statement concerning the,BTK-Bullet hole test ... The IDenta,s BTK- Bullet Hole Testing Kit was mentioned in news articles,on the ... To see the article, click on the ... http://www.lasvegassun.com/news/2008/jul/08/gee-whiz-packed-metro-csi-tru , , ...
... July 6, 2010 A new computer data compression ... Research Institute (TGen), will allow genetic researchers and others to ... space and at lower cost. Created specifically for genomic ... use are described in a paper published today in the ...
... known as quantum dots make ideal tools for distinguishing ... and Georgia Tech scientists have demonstrated. An article ... 15 issue of Analytical Chemistry describes how ... Reed-Sternberg cells that are characteristic of Hodgkin,s lymphoma. ...
Cached Biology News:International Drug & Explosives Detection Company IDenta Corp Reports on BTK- Bullet Hole Testing Kit 2International Drug & Explosives Detection Company IDenta Corp Reports on BTK- Bullet Hole Testing Kit 3New TGen technology reduces storage needs and costs for genomic data 2New TGen technology reduces storage needs and costs for genomic data 3Multicolor quantum dots aid in cancer biopsy diagnosis 2
(Date:9/19/2014)... Charlotte, NC (PRWEB) September 19, 2014 ... Certified Biobased Product Label for its Organic Cotton ... the product’s amount of renewable biobased ingredients meets or ... or intermediate materials composed in whole or in significant ... “This logo/label will allow our Organic cotton to be ...
(Date:9/19/2014)... The new , Healthcare and Life Sciences Review ... PharmaBoardroom.com for free download , looks beyond ... economy in 2009, and the austerity measures that followed, to ... times of trouble. "Despite the crisis, ... and the general conditions to invest are favorable to the ...
(Date:9/18/2014)... 2014 /PRNewswire-iReach/ -- DeepResearchReports.com adds "2014 Deep Research ... and "2014 Deep Research Report on Global and ... database. Photo - http://photos.prnewswire.com/prnh/20140918/147058 ... Global and China Acetic Acid Industry" is a ... China and Global Acetic Acid Market. ...
(Date:9/18/2014)... September 18, 2014 Texas Governor ... Sharp, Texas A&M Health Science Center CEO Brett ... of Health and Human Services (HHS), State of ... national pandemic influenza vaccine manufacturing facility in Bryan, ... anchor for the Texas A&M Biocorridor – a ...
Breaking Biology Technology:Barnhardt Manufacturing Company Earns USDA Certified Biobased Product Certification and Label for Organic Cotton 2PharmaBoardroom Releases New Spain Pharmaceuticals Report 2PharmaBoardroom Releases New Spain Pharmaceuticals Report 3Acetic Acid Market & Sodium Stannate Industry (China, Global) Research Report 2Acetic Acid Market & Sodium Stannate Industry (China, Global) Research Report 3Acetic Acid Market & Sodium Stannate Industry (China, Global) Research Report 4Texas A&M Dedicates National Pandemic Influenza Vaccine Manufacturing Facility, on Track for 2016 Start-Up Phase 2Texas A&M Dedicates National Pandemic Influenza Vaccine Manufacturing Facility, on Track for 2016 Start-Up Phase 3Texas A&M Dedicates National Pandemic Influenza Vaccine Manufacturing Facility, on Track for 2016 Start-Up Phase 4
... one of the rapidly growing markets in life science field. By ... to reach $2.2 billion. Major factors that continue to affect the ... and emerging technologies. , ... San Jose, CA (PRWEB) ...
... at it, the notion of harvesting energy from the sun ... ever. , Researchers at Rensselaer Polytechnic Institute have discovered and ... solar energy. By developing a new antireflective coating that boosts ... those panels to absorb the entire solar spectrum from nearly ...
... 3 Amgen (Nasdaq:,AMGN) today announced that it ... 7, 2008 at the Millennium Broadway Hotel, 145 ... a.m. Eastern Time. Participating in,the meeting will be ... Ph.D., executive vice president, Research and Development, George,Morrow, ...
Cached Biology Technology:World Gene Amplification Technologies Market to Reach $2.2 Billion by 2015, According to New Report by Global Industry Analysts, Inc. 2World Gene Amplification Technologies Market to Reach $2.2 Billion by 2015, According to New Report by Global Industry Analysts, Inc. 3Solar power game-changer: 'Near perfect' absorption of sunlight, from all angles 2Solar power game-changer: 'Near perfect' absorption of sunlight, from all angles 3Amgen to Webcast 2008 Business Review Meeting on November 7 From Millennium Hotel in New York 2
... Dr. P Set from different human ... More organs maybe available upon request. ... items from the whole set, and ... price. All Dr. P Set ...
... buffer and fixative results in complete ... Vivo Manual Perfusion Fixation System ... and fixative results in complete perfusion ... clean--Complete system with everything down to ...
Mouse IL-31 RA Biotinylated Affinity Purified PAb...
... plus assay system offers the ... Designed for batch-processing systems, Long ... density microplates such as 384-well ... Convenient storage conditions ( 2 ...
Biology Products: