Navigation Links
New method takes snapshots of proteins as they fold
Date:1/10/2011

"stills" taken by the camera slow motion to the point that normally imperceptible events are laid open to scrutiny.

The scientists are using a version of this old trick to "watch" proteins fold. The "strobe light" is a temperature jump and the "camera" is a fast chemical reaction whose outcome is measured by a sensitive mass spectrometer.

Why folding is a complex problem

One of the dogmas of modern biology is that the sequence of amino acids determines how a protein will fold. If the amino acid sequence is known, it should be possible to calculate the protein's final structure from scratch.

But like many things in life, it's harder than it looks.

"Think of a protein as thousands of atoms connected together by springs," says Gross, who is also director of the National Institutes of Health/ National Center for Research Resources (NIH/NCRR) Mass Spectrometry Resource "If you were to suspend this object with a string from the ceiling and let it flop around, imagine how many shapes it could take."

"An enormous number, because it is free to move in so many different ways."

In practice, scientists often predict protein structure not from scratch but by analogy. They sift through large databases for proteins with similar sequences of amino acids and assume similar amino-acid chains will fold in similar ways.

"But," says Gross, "at some point any method for predicting protein structure has to be checked against experimental evidence that shows how proteins actually do fold."

That's what his research is all about.

A model protein for the experiment

Barstar is a small protein synthesized by a soil bacterium that is often used in folding studies.

Importantly, barstar's "native state" is known, as is its primary structure, the sequence of the protein subunits called amino acids of which it is made. What isn't known is how the amino-acid chain twists and coils
'/>"/>

Contact: Diana Lutz
dlutz@wustl.edu
314-935-5272
Washington University in St. Louis
Source:Eurekalert  

Page: 1 2 3 4 5

Related biology news :

1. Scripps Research chemist devises new method to quantify protein changes
2. Transcriptome analysis, organ culture methods featured in Cold Spring Harbor Protocols
3. New method for making tiny catalysts holds promise for air quality
4. New labeling method expands ability to read DNA modification
5. MGH researchers develop faster method of engineering zinc-finger nucleases
6. Researcher develops accurate method for detecting dangerous fluoride
7. Shotgun method allows scientists to dissect cells sugar coatings
8. New low-cost method to deliver vaccine shows promise
9. Fast-track gene-ID method speeds rare disease search
10. OU study on genetics in fruit flies leads to new method for understanding brain function
11. New method successfully predicted how oil from Deepwater Horizon spill would spread
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
New method takes snapshots of proteins as they fold
(Date:7/29/2014)... wrong food at a restaurant, don,t blame yourself; blame the ... with what you want and more to do with a ... the selections of over 300 diners, the Cornell study published ... showed that when it comes to what you order ... the menu and how you imagine it will taste. , ...
(Date:7/29/2014)... debilitating effects of a stroke is an immensely ... as well as effective therapy. Advancing such knowledge ... the primary focus of research by Sergei Adamovich, ... the NJIT faculty more than a decade ago. ... substantial funding from sources such as the National ...
(Date:7/29/2014)... easier method to create DNAprotein conjugates. The method ... diseases. , DNA linked to proteins including ... be used in diagnostic techniques, nanotechnology and other ... DNA can be used for purposes such ... material. The method also provides easier access to ...
Breaking Biology News(10 mins):Menu secrets that can make you slim by design 2Continuing the quest for better stroke therapies 2Continuing the quest for better stroke therapies 3New method provides researchers with efficient tool for tagging proteins 2
... --- How do the French get away with a clean ... fats? Scientists have long suspected that the answer to the ... of a new study bring them closer to understanding why. ... Public Library of Science (PLoS) ONE, researchers from industry and ...
... York next week to discuss new approaches to one ... weather. Five years ago, Professor Peter Bernath, of ... the Atmospheric Chemistry Experiment (ACE) satellite mission. To ... University at the King,s Manor in York will review ...
... release is available in German . ... cormorants in order to reconcile nature conservation and fishing ... work at the European level, researchers from the Helmholtz ... journal Environmental Conservation. Furthermore, they suggest a five-step action ...
Cached Biology News:Substance in red wine found to keep hearts young 2Substance in red wine found to keep hearts young 3York symposium puts focus on chemical 'weather' 2The cormorant -- the 'black plague' or an example of successful species conservation? 2The cormorant -- the 'black plague' or an example of successful species conservation? 3The cormorant -- the 'black plague' or an example of successful species conservation? 4The cormorant -- the 'black plague' or an example of successful species conservation? 5
(Date:7/29/2014)... WA (PRWEB) July 29, 2014 The ... of the brain that are driving a revolution in ... a new peer-reviewed journal published by SPIE, the ... first issue coincides with the launch of the BRAIN ... features articles that lay out a vision for and ...
(Date:7/29/2014)... 29, 2014   Sequenom, Inc. (NASDAQ: ... innovative genetic analysis solutions, and Mayo Medical Laboratories ... in the United States , have ... and applications. "We have great appreciation for ... and we welcome the opportunity to partner with the ...
(Date:7/29/2014)... Calif. , July 29, 2014 Human ... and therapeutic company focused on extending the healthy, high ... Franz Och , Ph.D., an expert in machine ... Scientist. Och comes to HLI from Google where he ... Translate. He will report directly to HLI Co-Founder and ...
(Date:7/29/2014)... Cat: Can a particle be separated from its properties? ... , published the results of the first Cheshire Cat ... by Chapman University in Orange, CA, and Vienna University ... cat without a grin," thought Alice in Wonderland, "but ... thing I ever saw in my life!" Alice,s surprise ...
Breaking Biology Technology:SPIE Launches 'Neurophotonics' Journal with Special Section on BRAIN Initiative 2SPIE Launches 'Neurophotonics' Journal with Special Section on BRAIN Initiative 3SPIE Launches 'Neurophotonics' Journal with Special Section on BRAIN Initiative 4Sequenom Enters Into License Agreement With Mayo Medical Laboratories 2Sequenom Enters Into License Agreement With Mayo Medical Laboratories 3Sequenom Enters Into License Agreement With Mayo Medical Laboratories 4Franz Och, Ph.D., Expert in Machine Learning and Machine Translation, Joins Human Longevity, Inc. as Chief Data Scientist 2Franz Och, Ph.D., Expert in Machine Learning and Machine Translation, Joins Human Longevity, Inc. as Chief Data Scientist 3The quantum Cheshire cat: Scientists separate a particle from its properties 2The quantum Cheshire cat: Scientists separate a particle from its properties 3The quantum Cheshire cat: Scientists separate a particle from its properties 4
... program provides ministry with tools and information to sustain ... hepatitis C education, screening and testing, PHILADELPHIA, Aug. ... Tabernacle Baptist Church from Saturday,August 18 - Sunday, August ... virus (HCV), including risk factors, the importance of getting,tested ...
... TARRYTOWN, N.Y., Aug. 17 Emisphere,Technologies, Inc. (Nasdaq: ... to sell 2.0 million shares of common stock and ... a purchase price of $3.785,per unit. The five-year warrants ... anniversary of issuance at an exercise price of $3.948 ...
... Aug. 17 Keck Graduate Institute,(KGI) today announced ... vice,president for advancement following a national search. ... experience in fundraising,communications, marketing and business development working ... are thrilled to have someone of Kerry,s caliber ...
Cached Biology Technology:Roche Joins Forces With Enon Tabernacle Baptist Church to Provide Free Hepatitis C Testing to the Community 2Roche Joins Forces With Enon Tabernacle Baptist Church to Provide Free Hepatitis C Testing to the Community 3Emisphere Technologies, Inc. Announces Pricing of Registered Direct Offering 2KGI Names Kerry Howell Vice President for Advancement 2
... An entire pressurized perfusion system ... in physiology, biophysics, electrochemistry, or general ... as little as 100 microliters of ... eight solutions in milliseconds from a ...
Request Info...
384 Cluster Tube Racks...
The antibody recognizes the peptide sequence ESESQGPK (aa 441-448) of human class III beta-tubulin specific for neurones....
Biology Products: