Navigation Links
New method provides researchers with efficient tool for tagging proteins
Date:7/29/2014

Aarhus University researchers have developed an easier method to create DNAprotein conjugates. The method can potentially strengthen the work involved in diagnosing diseases.

DNA linked to proteins including antibodies provides a strong partnership that can be used in diagnostic techniques, nanotechnology and other disciplines. DNAprotein conjugates which tag proteins with DNA can be used for purposes such as the sensitive detection and visualisation of biological material. The method also provides easier access to handling proteins in nanotechnology, where the DNA acts as a handle on the protein.

Controlling the conjugation of macromolecules such as DNA and proteins can be quite a challenge when scientists want to join them in particular ways and places. Researchers at Aarhus University have now developed a new and efficient method to tag proteins with DNA, making it much simpler to control the process than previously. The new method was developed at the Danish National Research Foundation's Centre for DNA Nanotechnology (CDNA) in collaboration between researchers at Aarhus University's Interdisciplinary Nanoscience Centre (iNANO), Department of Chemistry and Department of Molecular Biology and Genetics. The work is described in the highly prestigious scientific journal Nature Chemistry.

"Maintaining the protein's function and activity often requires the attachment of only a single DNA strand to the protein. At the same time, it can be important to know where the DNA strand is attached to the protein. You can normally only achieve this if you are working with genetically engineered proteins. This is a time-consuming and technically challenging process," explains PhD student Christian B. Rosen, CDNA, Aarhus University one of the researchers behind the new method.

The new method makes it possible to direct the tagging of proteins with DNA to a particular site on the protein, without genetically modifying the protein beforehand. In other words, it is possible to tag natural proteins, including antibodies.

The researchers use a piece of DNA that is engineered to bind to metal ions. Using this 'control stick', they direct another piece of DNA to a metal binding site on the protein, where it reacts. A considerable number of proteins bind metal ions, which makes them suitable for this method. A significant point in using this method is that the tagged proteins retain their functionality after being bound to DNA.

The researchers are applying for a patent for the new method, which has potential in a number of areas.

"Of greatest importance is the fact that we can use our technique for tagging antibodies. Antibodies that are chemically bound (conjugated) to chemotherapeutics represent an entirely new class of medicine in which the antibody part is used to recognise specific tissue and the chemotherapeutic part is used to kill the cell. When you tag antibodies, it's important that you keep the recognition element of the antibody intact. With our method, we strike the constant part of the antibody and not the variable part, which contains its recognition element. Our technique is therefore general for a major class of proteins," explains Anne Louise Bank Kodal, CDNA, another author of the article.


'/>"/>

Contact: Christian B. Rosen
crosen@chem.au.dk
Aarhus University
Source:Eurekalert

Related biology news :

1. New method for reducing tumorigenicity in induced pluripotent stem-cell based therapies
2. Novel methods may help stem cells survive transplantation into damaged tissues
3. Splice-switching oligonucleotide therapeutics is new method for editing gene transcript
4. A new genome editing method brings the possibility of gene therapies closer to reality
5. Food allergies: A new, simple method to track down allergens
6. Comparison study of planting methods shows drilling favorable for organic farming
7. Researchers discover Trojan Horse method of penetrating cellular walls without harm
8. Animal testing methods for endocrine disruptors should change, team argues
9. Frederic Bushman, Ph.D. receives Pioneer Award for advancing therapeutic gene delivery methods
10. Fungal infection control methods for lucky bamboo
11. New method of wormlike motion lets gels wiggle through water
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/20/2016)... DALLAS , June 20, 2016 ... criminal justice technology solutions for public safety, investigation, ... by the prisons involved, it has secured the ... Corrections (DOC) facilities for Managed Access Systems (MAS) ... (4) additional facilities to be installed by October, ...
(Date:6/7/2016)... 2016  Syngrafii Inc. and San Antonio Credit ... includes integrating Syngrafii,s patented LongPen™ eSignature "Wet" solution ... will result in greater convenience for SACU members ... maintaining existing document workflow and compliance requirements. ... Highlights: ...
(Date:6/1/2016)... , June 1, 2016 ... in Election Administration and Criminal Identification to Boost Global ... a recently released TechSci Research report, " Global Biometrics ... Region, Competition Forecast and Opportunities, 2011 - 2021", the ... billion by 2021, on account of growing security concerns ...
Breaking Biology News(10 mins):
(Date:12/2/2016)... ... 02, 2016 , ... Robots will storm the Prudential Center in Boston, MA ... event, which is held on the United Nations International Day of Persons with Disabilities, ... the workplace. Suitable Technologies is partnering with NTI to showcase how technology can help ...
(Date:12/2/2016)... world leader in rapid infectious disease tests, introduced the Company,s newest product, the INSTI ... (Photo: http://photos.prnewswire.com/prnh/20161201/444905 ) Continue Reading ... ... , bioLytical was invited by the Clinton ... HIV Self Test to 350 pharmacy representatives in Nairobi and ...
(Date:11/30/2016)... , November 30, 2016 The global ... few players hold a dominant share in the overall ... Laboratories International, Inc., and Merck KGaA, held a lion,s ... Transparency Market Research observes that these companies are expected ... development products that are do not require rabbit pyrogen ...
(Date:11/30/2016)... , Nov. 30, 2016  The Allen Institute ... Collection: the first publicly available collection of gene ... that target key cellular structures with unprecedented clarity. ... these powerful tools are a crucial first step ... better understand what makes human cells healthy and ...
Breaking Biology Technology: