Navigation Links
New method provides panoramic view of protein-RNA interactions in living cells
Date:11/2/2008

ddress the issue, Darnell and his team used a trick from test-tube biochemistry that molecularly cements these regulatory proteins to RNA at the moment they touch. The technique, when applied to high throughput sequencing, is called high throughput sequencing-cross linking immunoprecipitation, or HITS-CLIP for short.

Since the RNA and RNA-binding protein are fused together, the researchers can really beat up the extract and rigorously purify the protein without fear of losing the RNA. At the end of the day, they are left with the RNA sequence to which the protein was bound. They can then take these sequences to Rockefeller's high throughput sequence facility, and with the help of Research Support Specialist Scott Dewell, overlay them onto the genome and see where they match. What they get is a map of every position on every transcribed RNA where the RNA binding protein is binding.

When DNA is transcribed into RNA, the primary transcript is divided into many blocks called exons, which are separated by empty spaces. In order to convert the transcript into some sort of message, all the spaces need to be removed; but if an exon is dropped, a different version of that protein, which could carry a very different message, is created. "That's RNA splicing," says first author Donny Licatalosi, a postdoctoral associate in the lab. "It is what gives rise to this massive pool of diverse and complex tissues with a relatively small number of genes."

In the past, the group used a sophisticated process of evidence and inference to make predictions of the points of regulation along the transcript. "Now, we have direct biochemical validation that these interactions occur in the brain to regulate splicing," says Licatalosi.

And as it turns out, "The observed map -- and this was amazing -- looked just like our predicted map," says Darnell.

Darnell, Licatalosi and their colleagues Aldo Mele, a research assistant, John Fak, a research assist
'/>"/>

Contact: Thania Benios
tbenios@rockefeller.edu
212-327-7146
Rockefeller University
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Iowa State researcher develops new treatment method for canine eye diseases
2. Fingerprinting method tracks mercury emissions from coal
3. Deep biosphere research points to new methods for recovering petroleum
4. New lab manual focuses on essential methods for purifying and characterizing proteins
5. UC Riverside biochemists devise method for bypassing aluminum toxicity effects in plants
6. Commercial aquatic plants offer cost-effective method for treating wastewater
7. Radioactivity: Discover the lowest amounts with new methods
8. New method identifies meth hot spots
9. Scientists develop new method to investigate origin of life
10. Biophysical method may help to recover hearing
11. LSUHSC research reports new method to protect brain cells from diseases like Alzheimers
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/27/2015)... more than four decades, AUVSI,s Unmanned Systems ... event for the unmanned land, sea, and air industries. ... local, national, or trade news organization interested in gathering ... applications of unmanned technologies. The conference will explore the ... and how it will soon impact the lives of ...
(Date:4/27/2015)... 27, 2015 Profile Solutions, Inc. (OTC: PSIQ), ... systems is pleased to announce that Dr Gerry ... and member of its scientific advisory board. ... a thought leader in technology-enhanced learning models. He was ... has published studies and books focused on online student ...
(Date:4/20/2015)... Calif. , April 20, 2015 Huntington ... Gabriel Valley to implant a new miniaturized, wireless monitoring ... System is the first and only FDA-approved heart failure ... hospital admissions when used by physicians to manage heart ... sensor that is implanted in the pulmonary artery (PA) ...
Breaking Biology News(10 mins):UNMANNED SYSTEMS 2015: Largest International Conference and Trade Show for the Drone and Unmanned Technology Industry 2UNMANNED SYSTEMS 2015: Largest International Conference and Trade Show for the Drone and Unmanned Technology Industry 3UNMANNED SYSTEMS 2015: Largest International Conference and Trade Show for the Drone and Unmanned Technology Industry 4UNMANNED SYSTEMS 2015: Largest International Conference and Trade Show for the Drone and Unmanned Technology Industry 5Profile Solutions Names Medicinal Cannabis, Agriculture and Technology Expert Dr. Gerry Bedore to its Scientific Advisory Board 2Profile Solutions Names Medicinal Cannabis, Agriculture and Technology Expert Dr. Gerry Bedore to its Scientific Advisory Board 3Profile Solutions Names Medicinal Cannabis, Agriculture and Technology Expert Dr. Gerry Bedore to its Scientific Advisory Board 4Huntington Memorial Hospital Becomes First in the San Gabriel Valley to Offer Patients a New Heart Failure Monitoring Solution 2Huntington Memorial Hospital Becomes First in the San Gabriel Valley to Offer Patients a New Heart Failure Monitoring Solution 3
... discovery of genetic differences affecting up to a third ... prescribing the correct dose of 25 percent of drugs ... found two genetic variants that alter the activity level ... ranging from the painkiller codeine to the breast cancer ...
... Cullen College of Engineering has won a grant to make ... safer and more effective. The grant goes to Nuri ... National Science Foundation, it is valued at $330,000 for three ... an M.D./Ph.D a neurosurgeon with the University of Colorado School ...
... developed, none has matched the complexity of the fundamental ... of the cell,s activities would be possible without thin ... their functions. Understanding and controlling bilayers, properties ... an interdisciplinary team of Northwestern University researchers has determined ...
Cached Biology News:New genetic discovery could reduce the guesswork in drug dosing 2New genetic discovery could reduce the guesswork in drug dosing 3New genetic discovery could reduce the guesswork in drug dosing 4Parkinson's Surgery Research wins NSF Grant 2Study: Acidity can change cell membrane properties 2Study: Acidity can change cell membrane properties 3
(Date:5/28/2015)... Research and Markets ... of Jain PharmaBiotech,s new report "Therapeutic ... to their offering.      ... deals with therapeutic drug monitoring, a multi-disciplinary ... by monitoring drug levels in the blood ...
(Date:5/28/2015)... May 27, 2015 Research and ... of Jain PharmaBiotech,s new report "Nanobiotechnology ... offering. An increasing use of ... is anticipated. Nanotechnology will be applied at ... formulations for optimal delivery to diagnostic applications ...
(Date:5/28/2015)... DUBLIN , May 27, 2015 ... addition of Jain PharmaBiotech,s new report "Neuroprotection ... offering. This report describes the role ... and injuries of the nervous system as well ... because many of the underlying mechanisms of damage ...
(Date:5/28/2015)... , May 28, 2015 Natera, Inc., ... a study published in Ultrasound in Obstetrics ... polymorphism (SNP)-based non-invasive prenatal test (NIPT) can identify ... A study based upon data ... pregnancies occur in about 1 in 1,000 pregnancies, ...
Breaking Biology Technology:Global Therapeutic Drug Monitoring Market 2015-2024 2Global Nanobiotechnology Market 2015-2024 - Applications, Markets and Companies 2Global Nanobiotechnology Market 2015-2024 - Applications, Markets and Companies 3Global Neuroprotection Market Report 2015-2024 - Drugs, Markets and Companies 2Global Neuroprotection Market Report 2015-2024 - Drugs, Markets and Companies 3First Confirmed Detection of a Complete Molar Pregnancy with Cell-Free DNA Test 2First Confirmed Detection of a Complete Molar Pregnancy with Cell-Free DNA Test 3
... , , , ... VOLC ), a leader in the development, manufacturing and sales of ... disease, announced today its participation in the 2009 Transcatheter Cardiovascular Therapeutics ... for TCT includes a first look at new study data, live ...
... ROCKVILLE, Md., Sept. 21 Neuralstem, Inc. (NYSE Amex: ... (FDA) has approved its Investigational New Drug (IND) application to commence a ... disease) with its spinal cord stem cells. , , ... Neuralstem is the first company to commence a stem cell ...
... , NES-ZIONA, Israel, Sept. 21 PROLOR Biotech, ... the initiation of a Phase I clinical trial of its ... hGH-CTP is being developed to provide growth hormone ... multiple injections per week that are currently required with a ...
Cached Biology Technology:Volcano Corporation Announces Schedule of Events for the 2009 Transcatheter Cardiovascular Therapeutics (TCT) Conference 2Volcano Corporation Announces Schedule of Events for the 2009 Transcatheter Cardiovascular Therapeutics (TCT) Conference 3Volcano Corporation Announces Schedule of Events for the 2009 Transcatheter Cardiovascular Therapeutics (TCT) Conference 4Volcano Corporation Announces Schedule of Events for the 2009 Transcatheter Cardiovascular Therapeutics (TCT) Conference 5Volcano Corporation Announces Schedule of Events for the 2009 Transcatheter Cardiovascular Therapeutics (TCT) Conference 6Neuralstem Receives FDA Approval to Commence First ALS Stem Cell Trial 2Neuralstem Receives FDA Approval to Commence First ALS Stem Cell Trial 3Neuralstem Receives FDA Approval to Commence First ALS Stem Cell Trial 4PROLOR Biotech Announces Initiation of Phase I Clinical Trial for its Long-Acting Growth Hormone 2PROLOR Biotech Announces Initiation of Phase I Clinical Trial for its Long-Acting Growth Hormone 3PROLOR Biotech Announces Initiation of Phase I Clinical Trial for its Long-Acting Growth Hormone 4
Umbilical cord blood basophils, For immunohistochemistry (IHC)...
Human peripheral blood neutrophils, For immunohistochemistry (IHC) Cell Chip with Human Peripheral Blood Granulocytes...
Human peripheral blood CD14+ monocytes, For immunohistochemistry (IHC) Cell Chip with Human Peripheral Blood Monocytes, B Cells, NK Cells and Stem Cells...
... HT96 Isothermal Block is an anodized aluminum, ... plates and to provide efficient thermal transfer ... new design of the HT96 Isothermal Block ... enhanced product design allowing for compatibility with ...
Biology Products: