Navigation Links
New method isolates immune cells for researchers to study how they ward off oral diseases
Date:4/15/2014

Case Western Reserve University dental researchers have found a less invasive way to extract single rare immune cells from the mouth to study how the mouth's natural defenses ward off infection and inflammation.

By isolating some specialized immune cells (white blood cells known as "leukocytes") to study how they fight diseases in the mouthor reject foreign tissues, such as in failed organ transplantsresearchers hope to learn more about treating and preventing such health issues as oral cancers, cardiovascular disease, AIDS and other infectious diseases.

To this point, researchers have had to rely on studying and growing immune cells from blood. Studying tissue immune cells allows researchers to learn how they function at the site of infection.

The role of adaptive immune cells in the stomach and intestines is more widely known, yet the role of similar cells in the mouth is unclear. There are no reliable methods to extract immune cells from mouth, which are more accessible and easier to extract than harder-to-reach tissues in the stomach and intestines.

But, until now, immune cells removed from the mouth couldn't be isolated with enough viability or grown to study their activities, Pushpa Pandiyan, assistant professor of biological sciences at the dental school, explained.

The new method, developed by Pandiyan, the study's lead author, is described in Biological Procedures article, "Isolation of T cells from mouse oral tissues."

Pandiyan, who studies oral diseases associated with HIV, found no reliable method existed to isolate and keep a single cell from the tongue, gums and palate alive long enough to study.

Pandiyan and her team developed a way to do so successfully. The researchers reported that more than 94 percent of the isolated cells lived long enough to study.

Their method

Using mouse models, the researchers isolated two important specialized immune T lymphocytes that play a role in fighting oral diseases. The cells are part of the adaptive immune system in which cells respond to pathogens invading the body.

The researchers took tissue samples from the mouths of mice and washed them several times in saline and chemical solutions with antibiotics. This was followed by disintegrating the tissue using salts and enzymes. The solution was centrifuged and strained to separate different tissue parts with more washings and separations before the cells could be studied and grown.

Pandiyan, who received an early career travel award from the American Association of Immunologists (AAI) will present her findings at the organization's annual meeting, May 2-7, in Pittsburgh.

Natarajan Bhaskaran, Yifan Zhang and Aaron Weinberg, from Case Western Reserve University's Department of Biological Sciences, contributed to the study, which was funded by the university's dental school.


'/>"/>

Contact: Susan Griffith
susan.griffith@case.edu
216-368-1004
Case Western Reserve University
Source:Eurekalert

Related biology news :

1. New method confirms humans and Neandertals interbred
2. Gauging the impact of tropical forest logging: Winrock develops new method for quantifying carbon emissions
3. Research method integrates meditation, science
4. Faster genetic testing method will likely transform care for patients with breast cancer
5. New method yields potent, renewable human stem cells with promising therapeutic properties
6. A non-invasive, rapid screening method for Alzheimers disease
7. AZTI-Tecnalia develops a methodology for authenticating canned tuna species within 24 hours
8. Researchers warn against abrupt stop to geoengineering method
9. New NIST method evaluates response to oxidation in live cells
10. Caltech-developed method for delivering HIV-fighting antibodies proven even more promising
11. New method developed for ranking disease-causal mutations within whole genome sequences
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:11/15/2016)... Nov 15, 2016 Research and Markets has ... to 2021" report to their offering. ... ... Billion by 2021 from USD 6.21 Billion in 2016, growing at ... Growth of the bioinformatics market is driven by the growing demand ...
(Date:6/22/2016)... -- The American College of Medical Genetics and Genomics was once ... one of the fastest-growing trade shows during the Fastest 50 ... Las Vegas . Winners ... each of the following categories: net square feet of paid ... The 2015 ACMG Annual Meeting was ranked 23 out of ...
(Date:6/20/2016)... , June 20, 2016 Securus ... justice technology solutions for public safety, investigation, corrections ... the prisons involved, it has secured the final ... (DOC) facilities for Managed Access Systems (MAS) installed. ... additional facilities to be installed by October, 2016. ...
Breaking Biology News(10 mins):
(Date:12/2/2016)... ... December 01, 2016 , ... DrugDev believes the ... and a beautiful technology experience. All three tenets were on display at the 2nd ... leaders from over 40 sponsor, CRO and site organizations to discuss innovation and the ...
(Date:12/2/2016)... ... December 02, 2016 , ... Robots will storm the Prudential Center ... 3rd, 2016. The event, which is held on the United Nations International Day of ... Disabilities back into the workplace. Suitable Technologies is partnering with NTI to showcase how ...
(Date:12/2/2016)... Dec. 2, 2016 /PRNewswire/ - bioLytical Laboratories, a world leader in rapid infectious disease ... Kenya,s Pharmaceutical Association members. (Photo: http://photos.prnewswire.com/prnh/20161201/444905 ) ... ... , , ... Kenya Pharmaceutical Association (KPA) to introduce the INSTI HIV Self Test to 350 pharmacy ...
(Date:11/30/2016)... 2016 The global Pyrogen Testing ... a dominant share in the overall market. The leading ... and Merck KGaA, held a lion,s share of 51% ... observes that these companies are expected to retain their ... are do not require rabbit pyrogen testing along with ...
Breaking Biology Technology: