Navigation Links
New method for rapidly producing protein-polymers
Date:1/25/2011

them iteratively into a bacterial plasmid," Amiram said. "After the vector has grown in size, the copies of the sequence are cut out using enzymes and the process is repeated to generate a larger polymer. It is a very time-consuming process.

"With this new method, you don't get just one product, but many," she said. "This should help us to make large libraries of proteins, which we can use to rapidly screen new combinations. This powerful strategy generates libraries of repetitive genes over a wide range of molecular weights in a 'one-pot' parallel format."

Chilkoti compared it to sausage-making. Instead of stuffing the casings one-by-one individually, the new tool can rapidly stuff and stitch together long strings of sausages.

"This could help remove one of the biggest stumbling blocks we face in producing these drugs," Chilkoti said. "You can't make the proteins without genes, which act as the software directing the protein's production. Instead of building each sequence individually, as is done now, we can literally make hundreds, each with subtle differences."

The researchers used the system to synthesize genes found in two classes of protein-polymers. In the first, they produced protein-polymer combinations for elastin, a ubiquitous protein found in connective tissue. The researchers term them "smart" protein-polymers because they can be controlled by heat.

In the second set of experiments, they rapidly synthesized novel glucagon-like peptide-1 (GLP-1) analogs to show variable pharmokinetic properties. GLP-1 is a hormone that acts to release insulin in the body.


'/>"/>

Contact: Richard Merritt
richard.merritt@duke.edu
919-660-8414
Duke University
Source:Eurekalert  

Page: 1 2

Related biology news :

1. Commercial aquatic plants offer cost-effective method for treating wastewater
2. UC Riverside biochemists devise method for bypassing aluminum toxicity effects in plants
3. New lab manual focuses on essential methods for purifying and characterizing proteins
4. Deep biosphere research points to new methods for recovering petroleum
5. Fingerprinting method tracks mercury emissions from coal
6. Iowa State researcher develops new treatment method for canine eye diseases
7. New method provides panoramic view of protein-RNA interactions in living cells
8. Cold Spring Harbor Protocols features organ and cell culture methods
9. Engineer to present leak-proof method for carbon dioxide storage at international conference
10. Pitt researchers use fluorescence to develop method for detecting mercury in fish
11. New holographic method could be used for lab-on-a-chip technologies
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
New method for rapidly producing protein-polymers
(Date:12/10/2014)... 9, 2014     ...   Jifflenow, a ... solutions for business-to-business (B2B) events, today announced a ... mobile near-field communication (NFC), Bluetooth low energy (BLE), ... http://photos.prnewswire.com/prnh/20140612/691055) , Jifflenow will integrate its ...
(Date:12/5/2014)... , Dec. 4, 2014  Tute Genomics, a leader ... million in Series A1 funding led by UK-based Eurovestech. ... participated in the investment round. "We are ... community adopts next-generation sequencing and seeks new approaches for ... Reid Robison , MD MBA, and CEO of Tute ...
(Date:12/3/2014)... , Dec. 2, 2014   Marvin Test ... innovative test solutions for military, aerospace, and manufacturing ... of its successful TS-900 PXI semiconductor test ... features of high-end systems to customers at a ... compared to traditional ATE. "Our ...
Breaking Biology News(10 mins):Jifflenow And ITN International Bring Cutting-Edge Badge Scanning Technology To B2B Events 2Jifflenow And ITN International Bring Cutting-Edge Badge Scanning Technology To B2B Events 3Tute Genomics Raises $2.3 Million to Scale Up Cloud-Based Genome Analytics and Accelerate Personalized Medicine 2Tute Genomics Raises $2.3 Million to Scale Up Cloud-Based Genome Analytics and Accelerate Personalized Medicine 3Marvin Test Solutions Brings New Capabilities to PXI-Based Semiconductor Test with TS-960 2Marvin Test Solutions Brings New Capabilities to PXI-Based Semiconductor Test with TS-960 3
... Orleans, LA Research led by Wayne L. Backes, PhD, ... Health Sciences Center New Orleans School of Medicine, has found ... present in an individual, but also how they interact, and ... safely eliminated from the body or is converted into a ...
... Pa. , March 17 Orbel,s ... design your own PCB shield. Visit the BLS section ... from a wide variety of surface mount or ... The easy-to-use application lets you enter shield dimensions, ...
... A new study involving scientists from 13 different ... protection offers one of the most effective, practical, ... study, "Indigenous Lands, Protected Areas, and Slowing Climate ... a peer-reviewed scientific journal, and makes specific recommendations ...
Cached Biology News:LSUHSC research increases understanding of drug metabolism 2Orbel Introduces the Board Shielding Wizard 2Study highlights forest protected areas as a critical strategy for slowing climate change 2
(Date:12/24/2014)... SARASOTA, Fla. , Dec. 23, 2014  Rock ... today that it has filed a Clinical Trial Application ... Medicines Healthcare products Regulatory Agency (MHRA) seeking regulatory approval ... its lead molecule, Anatabine Citrate. Contingent on ... to conduct a Phase I trial to assess the ...
(Date:12/22/2014)... , Dec. 22, 2014  Alternative Energy ... that it has signed a letter of intent ... has developed and patented a nanotechnology-based development platform ... products that enable rapid on-site collection and testing ... and health issues in an immediate, non-invasive and ...
(Date:12/22/2014)... (PRWEB) December 22, 2014 Fertility Associates ... Donor Egg Bank USA , a network of more ... Through this partnership, patients have fast access to a ... vitro fertilization, IVF . Fertility Associates of Memphis is ... this fertility treatment option. , Dr. William Kutteh ...
(Date:12/19/2014)... Calif. , Dec. 19, 2014  Roche (SIX: ... of Bina Technologies, Inc. (Bina), a privately held ... , USA. Bina provides a big data ... generation sequencing (NGS) data. Bina,s proprietary on-market Genomic ... and academic researchers to perform fast and scalable ...
Breaking Biology Technology:Rock Creek Pharmaceuticals Files Clinical Trial Application in the UK for Clinical Trials of Anatabine Citrate Formulations 2Rock Creek Pharmaceuticals Files Clinical Trial Application in the UK for Clinical Trials of Anatabine Citrate Formulations 3ALNE Announces Intention To Acquire BioTechPharma 2Fertility Associates of Memphis Announces Partnership with Donor Egg Bank USA 2Fertility Associates of Memphis Announces Partnership with Donor Egg Bank USA 3Roche acquires Bina Technologies and enters the genomic informatics market 2Roche acquires Bina Technologies and enters the genomic informatics market 3
... Boehringer Ingelheim today,announced that the company ... as the,world,s most respected biopharmaceutical employer. Science,s ... polls employees in the biotechnology,biopharmaceutical, pharmaceutical, and ... asked to rate companies based on 23 ...
... featured in oral presentation ... TSX Exchange Symbol: ... is pleased to announce that tomorrow it is making,an oral ... at the International Atherosclerosis Society (IAS),Workshop on HDL. The presentation ...
... New sol-gel inks developed by researchers at the University ... three-dimensional structures of metal oxides with nanoscale features. , ... nanoscale opens a new avenue to functional devices. Potential ... The researchers describe the new inks in a paper ...
Cached Biology Technology:Boehringer Ingelheim Receives Top Honor as World's Most Respected Biopharmaceutical Employer in Science 2007 Top Employers Survey 2Boehringer Ingelheim Receives Top Honor as World's Most Respected Biopharmaceutical Employer in Science 2007 Top Employers Survey 3Resverlogix is Presenting at IAS HDL Workshop 2Sol-gel inks produce complex shapes with nanoscale features 2
... through 63 ligand binding ... a broad therapeutic range. ... many non human tissue ... assays. Used to ...
...
... Sequencer 20 System revolutionizes DNA sequencing, delivering ... The Genome Sequencer 20 System includes: ... and consumables for library construction, amplification, and ... de novo assembly The ...
... to the maintenance of appropriate levels ... important and diverse as those involved ... stress response, antigen presentation, modulation of ... cell cycle regulation, transcription, and signalling ...
Biology Products: