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New method for diagnosing malaria
Date:11/27/2012

Malaria is a life-threatening disease that strikes more than 200 million people every year mainly in Africa, Asia and Latin America. The disease is caused by the Plasmodium parasite, which is spread by infected mosquito bites. Today, malaria can be prevented and successfully treated, but more than half a million people nevertheless die every year from the disease.

Large-scale monitoring and treatment programmes during the past decade have reduced the distribution of the disease, and the frequency of actual epidemics has fallen. However, the number of malaria patients with relatively low infection counts has increased, and the need for more sensitive methods to diagnose the disease has thus increased dramatically.

Development of new, simple and sensitive method

To meet this need, researchers at Aarhus University have developed a new method that can diagnose malaria infections with very high sensitivity. The method is based on measuring the activity of an enzyme called topoisomerase I from the Plasmodium parasite.

The researchers have developed a technology called REEAD (Rolling Circle-Enhanced Enzyme Activity Detection) which makes it possible to diagnose malaria from a single drop of blood or saliva. This method is much more time-effective and cost-effective than current diagnostic methods, and can be performed by personnel who have no specialised training. It can therefore be used in low-resource areas without the use of expensive equipment, clean water or electricity.

The ongoing fight against malaria is complicated by increasing problems with resistant Plasmodium parasites. In addition, several Plasmodium species (P. vivax and P. knowlesi) cannot be detected with the usual quick-test methods.

The new REEAD-based method distinguishes itself from other quick-test methods because it can measure whether a given Plasmodium infection is resistant to dr
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Contact: Birgitta R. Knudsen
brk@mb.au.dk
45-60-20-26-73
Aarhus University
Source:Eurekalert  

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