Navigation Links
New method for detection of phosphoproteins reveals regulator of melanoma invasion
Date:4/9/2009

Scientists have developed a new approach for surveying phosphorylation, a process that is regulated by critical cell signaling pathways and regulates several key cellular signaling events. The research, published by Cell Press in the April 10th issue of the journal Molecular Cell, describes the regulation of a previously uncharacterized protein and demonstrates that it plays an important role in cancer cell invasion.

Many cancers, including melanoma, are associated with mutations in the gene encoding the protein kinase B-Raf. Kinases are proteins that regulate the function of other proteins by attaching a phosphate group to them. B-RAF mutations often lead to dysregulation of protein phosphorylation by the mitogen-activated protein (MAP) kinase signaling pathway. Identification and characterization of MAP kinase target proteins is critical for understanding the mechanisms involved in cancer progression.

"In contrast to targets regulated at the level of gene expression, little is known about how proteins are modified in response to oncogenic B-Raf signaling in melanoma cells. In particular, identifying cellular targets for phosphorylation is needed to gain a more comprehensive understanding of the responses to MAP kinase pathway dysregulation in melanoma," explains senior study author Dr. Natalie G. Ahn from the Department of Chemistry and Biochemistry at the University of Colorado and the Howard Hughes Medical Institute.

Current strategies to identify phosphorylated proteins require purification techniques to enrich phosphorylated from non-phosphorylated proteins and metabolic labeling procedures to quantify changes in phosphorylation. Unfortunately, these methods are not readily applied to all sample types. Dr. Ahn and colleagues developed a method for analyzing phosphorylated proteins in human cell extracts that does not depend on enrichment and can be performed quantitatively in a label-free manner.

Using their method, the researchers identified ninety phosphorylation events that were regulated by oncogenic B-Raf. The phosphorylated proteins included many known signaling molecules. However, one of the targets, MINERVA/FAM129B, belonged to a protein family with unknown function. Further investigation established a role for MAP kinase-dependent phosphorylation of MINERVA/FAM129B in cancer cell invasion within a three dimensional extracellular matrix environment.

"Our results revealed successful selection and sequencing of phosphopeptides in proteolytic digests without affinity enrichment, as well as label-free quantitation of regulated protein phosphorylation events," concludes Dr. Ahn. "Further, we demonstrated pathway-dependent phosphorylation of FAM129B and discovered its importance in controlling melanoma cell invasion."


'/>"/>

Contact: Cathleen Genova
cgenova@cell.com
617-397-2802
Cell Press
Source:Eurekalert

Related biology news :

1. Scientists develop method for comprehensive proteome analysis
2. Engineers develop method to disperse chemically modified graphene in organic solvents
3. Pilot study shows effectiveness of new, low-cost method for monitoring hand hygiene compliance
4. New method for detecting explosives
5. Genomatix gets patent for comparative genomics method
6. Protein function and chromatin structure methods featured in Cold Spring Harbor Protocols
7. New method monitors critical bacteria in wastewater treatment
8. Cold Spring Harbor Protocols features stem cell differentiation, plant RNAi methods
9. Intervention method reduces binge drinking
10. NYU scientists discover dangerous new method for bacterial toxin transfer
11. Methods for studying DNA repair and protein modification are featured in CSH Protocols
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/31/2016)... PROVIDENCE, R.I. , March 31, 2016  Genomics ... leadership of founding CEO, Barrett Bready , M.D., ... addition, members of the original technical leadership team, including ... Vice President of Product Development, Steve Nurnberg and Vice ... have returned to the company. Dr. Bready ...
(Date:3/23/2016)... March 23, 2016 ... Sicherheit Gesichts- und Stimmerkennung mit Passwörtern ... (NASDAQ: MESG ), ein führender Anbieter ... Unternehmen mit SpeechPro zusammenarbeitet, um erstmals dessen ... wird die Möglichkeit angeboten, im Rahmen mobiler ...
(Date:3/22/2016)... , PROVO and ... Newborn Screening Ontario (NSO), which operates the highest sample ... molecular testing, and Tute Genomics and UNIConnect, leaders in ... respectively, today announced the launch of a project to ... (NGS) testing panel. NSO has been ...
Breaking Biology News(10 mins):
(Date:5/18/2016)... ... May 18, 2016 , ... Every day, more than ... asthma complications.* Costing more than $56 billion in direct costs annually, asthma remains ... too many, the suffering associated with uncontrolled asthma can be overwhelmingly disproportionate and ...
(Date:5/17/2016)... ... 2016 , ... DryLet, LLC, a biotechnology company providing an ... be showcasing ManureMagic™ at booth V1061 at the World Pork Expo, June 8-10 ... last year and more recently made news as the results were released from ...
(Date:5/17/2016)... , May 17, 2016 ... in Basel, Switzerland announced ... inhibitor of P38 mitogen-activated protein kinase. ... Strekin will build the necessary research foundation ... in which MAP Kinases play fundamental roles. Pamapimod ...
(Date:5/17/2016)... ... May 17, 2016 , ... New tests that go ... country promising more specific information that both the patient and doctor want and need ... tests, presented at the American Urological Association’s annual meeting in San Diego, involve using ...
Breaking Biology Technology: