Navigation Links
New method for detection of phosphoproteins reveals regulator of melanoma invasion
Date:4/9/2009

Scientists have developed a new approach for surveying phosphorylation, a process that is regulated by critical cell signaling pathways and regulates several key cellular signaling events. The research, published by Cell Press in the April 10th issue of the journal Molecular Cell, describes the regulation of a previously uncharacterized protein and demonstrates that it plays an important role in cancer cell invasion.

Many cancers, including melanoma, are associated with mutations in the gene encoding the protein kinase B-Raf. Kinases are proteins that regulate the function of other proteins by attaching a phosphate group to them. B-RAF mutations often lead to dysregulation of protein phosphorylation by the mitogen-activated protein (MAP) kinase signaling pathway. Identification and characterization of MAP kinase target proteins is critical for understanding the mechanisms involved in cancer progression.

"In contrast to targets regulated at the level of gene expression, little is known about how proteins are modified in response to oncogenic B-Raf signaling in melanoma cells. In particular, identifying cellular targets for phosphorylation is needed to gain a more comprehensive understanding of the responses to MAP kinase pathway dysregulation in melanoma," explains senior study author Dr. Natalie G. Ahn from the Department of Chemistry and Biochemistry at the University of Colorado and the Howard Hughes Medical Institute.

Current strategies to identify phosphorylated proteins require purification techniques to enrich phosphorylated from non-phosphorylated proteins and metabolic labeling procedures to quantify changes in phosphorylation. Unfortunately, these methods are not readily applied to all sample types. Dr. Ahn and colleagues developed a method for analyzing phosphorylated proteins in human cell extracts that does not depend on enrichment and can be performed quantitatively in a label-free manner.

Using their met
'/>"/>

Contact: Cathleen Genova
cgenova@cell.com
617-397-2802
Cell Press
Source:Eurekalert

Page: 1 2

Related biology news :

1. Scientists develop method for comprehensive proteome analysis
2. Engineers develop method to disperse chemically modified graphene in organic solvents
3. Pilot study shows effectiveness of new, low-cost method for monitoring hand hygiene compliance
4. New method for detecting explosives
5. Genomatix gets patent for comparative genomics method
6. Protein function and chromatin structure methods featured in Cold Spring Harbor Protocols
7. New method monitors critical bacteria in wastewater treatment
8. Cold Spring Harbor Protocols features stem cell differentiation, plant RNAi methods
9. Intervention method reduces binge drinking
10. NYU scientists discover dangerous new method for bacterial toxin transfer
11. Methods for studying DNA repair and protein modification are featured in CSH Protocols
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/11/2014)... Mineral Research awarded Michael F. Holick, PhD, MD, of ... Louis V. Avioli Award. Holick, a professor of medicine, ... revolutionizing the understanding of vitamin D and its role ... member of the American Society for Bone and Mineral ... basic research. It is named for ASBMR,s first president ...
(Date:7/11/2014)... Cell scientists have set a "mouse TRAP" to capture ... a recent study published in the Journal of ... a technique called TRAP to extract cellular and genetic ... by scientists at the Rockefeller Institute for Medical Research ... the protein-making machinery, or ribosomes, of the cell type ...
(Date:7/11/2014)... Researchers from Salk Institute for Biological Studies, BGI, ... the safety and reliability of the existing targeted ... method, TALEN-HDAdV, which could significantly increased gene-correction efficiency ... study published online in Cell Stell Cell ... cell-based gene therapy. , The combination of stem ...
Breaking Biology News(10 mins):BUSM's Michael F. Holick receives American Society for Bone and Mineral Research award 2A new genome editing method brings the possibility of gene therapies closer to reality 2
... (September 25, 2007) Stem cell therapy for cardiac ... media will be the focus of the keynote addresses ... Treatment of Cardiovascular Disease conference. Conducted by Cedars-Sinai Medical ... and 5, the conference is co-sponsored by the California ...
... For several months last spring, the Vanderbilt greenhouse held ... than are known to exist in the wild., This ... by Jennifer Ellis, a doctoral student in the biological ... E. McCauley., The species is called the giant whorled ...
... Cryptochromes, which fulfill the molecular requirements for sensing the ... neurons of migratory birds (Mouritsen et al., PNAS, 2004). ... brain are active when the birds use their magnetic ... and a forebrain region (Cluster N; Mouritsen et al., ...
Cached Biology News:Cardiologists and heart surgeons meet for 'Controversies and Advances' conference 2Cardiologists and heart surgeons meet for 'Controversies and Advances' conference 3Student study bolsters case for adding a rare sunflower to the endangered species list 2
(Date:7/10/2014)... journal PNAS (Proceedings of the National Academy of ... sleep deprivation have a significant effect on our metabolism. ... best time of day to test for diseases such ... effectively. , Researchers from the University of Surrey and ... between sleep deprivation, body clock disruption and metabolism, and ...
(Date:7/10/2014)... our laptops and electric vehicles could store more energy ... help of a sponge-like silicon material. , Researchers ... used in one of the battery,s electrodes, as silicon ... of graphite. A paper describing the material,s performance as ... Nature Communications . , "Silicon has long been sought ...
(Date:7/10/2014)... the University of Illinois at Urbana-Champaign provides new ... of spin and heat at the nanoscale, and ... for data storage and information processing. , ... momentum. In a typical charge current, electrons, spin-angular-momentum ... explained David Cahill, a professor of materials science ...
(Date:1/15/2014)... , "The interface is the device," Nobel laureate ... properties to be found at the junctures where ... world of nanotechnology, the interfaces between layers of ... in such high-tech favorites as spintronics, high-temperature superconductors, ...
Breaking Biology Technology:Time of day crucial to accurately test for diseases, new research finds 2Silicon sponge improves lithium-ion battery performance 2Silicon sponge improves lithium-ion battery performance 3University of Illinois study advances limits for ultrafast nano-devices 2A deeper look at interfaces 2
... launched interactive, personalized services for Milwaukee drivers through ... announced Thursday. Traffic.com will provide area commuters with ... roadway traffic conditions including congestion levels, incidents, travel ... news release. , ,Data for the site will ...
... Madison, Wis. - While human stem cells offer potential ... the more immediate benefit from research into such cells will ... an area that for many years has been steeped in ... research since disease works within the context of cells. , ...
... - Healthcare professionals will have the opportunity hear about ... profession at the Wisconsin Dairyland HIMSS Fall Technology Conference ... the Radisson hotel in La Crosse, will be split into ... events such as a golf outing and a riverboat cruise, ...
Cached Biology Technology:Human stem cells called great opportunity for drug discovery 2Human stem cells called great opportunity for drug discovery 3Conference will review healthcare IT 2
... is a major step forward in ... handling system - with one universal ... modules. All five single channel and ... one handle. The lightweight handle is ...
alpha-N-Catenin Antibody Shipping Temperature: HOT Storage Temperature: -20C...
... EC3 Darkroom is designed for imaging of ... EC3 systems offer direct control for users ... settings by hand , A range of ... system for all fluorescent and chemiluminescent applications ...
... is designed for multiple user laboratories for ... TLC applications. The MultiDoc-It is available without ... provides a darkroom environment and protects the ... image capture and enhancement , The ...
Biology Products: