COLD SPRING HARBOR, N.Y. (Dec. 18, 2008) "Life happens at the level of proteins . . . They build, process, activate, and inactivate; they polymerize, repair, support, modify, degrade, fold, migrate, and transport; they shorten, signal, cleave, inhibit, digest, fluoresce, induce, excise, carry, and repress; they bind, transfer, translocate, amplify, proofread, regulate, and perform countless more activities."
This excerpt is from the preface of the new book Proteomics: A Cold Spring Harbor Laboratory Course Manual. Because proteins have such diverse responsibilities in the cell and are structurally and chemically diverse, scientists who are involved in the large-scale study of proteins require especially rigorous and reliable laboratory methods to successfully address their research questions.
Proteomics assembles cutting-edge protocols, helpful hints, and lecture notes to teach researchers from a wide variety of disciplines the essential methods of proteomics using state-of-the-art instrumentation. It is geared towards all involved in studying proteins, their interactions and characteristics in the laboratory, and can be used both as the basis for a course and as a detailed bench manual.
Its authors, Drs. Andrew J. Link and Joshua LaBaer, have co-taught a popular proteomics course at Cold Spring Harbor Laboratory for the past 7 years. Most of the experiments that have been taught in this course, as well as background descriptions and lists of recommended websites and reading material, are included in this manual. Both authors are leaders in their fields and provide complementary expertise.
The protocols include a range of basic and advanced methods for protein preparation, separation, and characterization. State-of-the-art methods involving protein microarrays, liquid chromatography, high-throughput cloning of expression constructs, IMAC, mass spectrometry, MALDI-TOF, and MudPIT are provided. An emphasis is placed on strategies for analyzing the data that are produced from each of the experiments.
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Cold Spring Harbor Laboratory