Navigation Links
New manual from Cold Spring Harbor Laboratory Press on culturing neural cells
Date:10/25/2013

Cell culture systems for specific neural cell types are essential for studies of their development and function.

Purifying and Culturing Neural Cells: A Laboratory Manual provides step-by-step protocols for isolating specific cell populations from rodent tissues and culturing them under conditions that closely resemble those in vivo. The contributors describe in detail how to dissect the brain, spinal cord, and other tissues; how to separate cells using mechanical and enzymatic tissue-dissociation strategies; the use of immunopanning and fluorescence-activated cell sorting (FACS) to enrich the target cell population; and the culture conditions that optimize cell viability and growth. Retinal ganglion cells, motor neurons, dorsal root ganglion cells, astrocytes, oligodendrocytes, and Schwann cells are covered, as are vascular cells such as pericytes and endothelial cells. Myelinating co-cultures of neurons and oligodendrocytes are also described.

The manual includes detailed recipes for media and reagents, tips for avoiding common pitfalls, and advice for designing new immunopanning protocols using tissues from other sources. Many of the protocols are accompanied by freely accessible online movies that demonstrate critical steps of the procedures. This is an essential laboratory companion for all neurobiologists, from the graduate student level upwards.


'/>"/>

Contact: Robert Redmond
rredmond@cshl.edu
516-422-4101
Cold Spring Harbor Laboratory
Source:Eurekalert  

Page: 1

Related biology news :

1. The most celebrated lab manual in molecular biology has been updated and expanded
2. Mid-Atlantic suburbs can expect an early spring thanks to the heat of the big city
3. Annual Drug Discovery Conferences Being Held in Boston MA, Spring 2012
4. An early spring drives butterfly population declines
5. Why spring is blooming marvelous (and climate change makes it earlier)
6. First fruitful, then futile: Ammonites or the boon and bane of many offspring
7. Springer and the Beijing Normal University Press sign agreement on new book series
8. Springer will collaborate with the Italian Society for Ultrasound in Medicine and Biology
9. Genetics Society of America announces Spring 2013 DeLill Nasser Travel Award recipients
10. Fetal exposure to PVC plastic chemical linked to obesity in offspring
11. In the Eastern US, spring flowers keep pace with warming climate
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
New manual from Cold Spring Harbor Laboratory Press on culturing neural cells
(Date:4/13/2017)... , April 13, 2017 UBM,s Advanced ... will feature emerging and evolving technology through ... Innovation Summits will run alongside the expo portion of ... sessions, panels and demonstrations focused on trending topics within ... advanced design and manufacturing event will take place June ...
(Date:4/11/2017)... , April 11, 2017 Crossmatch®, ... secure authentication solutions, today announced that it has ... Advanced Research Projects Activity (IARPA) to develop next-generation ... program. "Innovation has been a driving ... Thor program will allow us to innovate and ...
(Date:4/11/2017)... N.Y. , April 11, 2017 ... fingerprints, but researchers at the New York University ... College of Engineering have found that partial similarities ... security systems used in mobile phones and other ... thought. The vulnerability lies in the ...
Breaking Biology News(10 mins):
(Date:10/9/2017)... (PRWEB) , ... October 09, 2017 , ... At its ... Dr. Christopher Stubbs, a professor in Harvard University’s Departments of Physics and Astronomy, has ... was a member of the winning team for the 2015 Breakthrough Prize in Fundamental ...
(Date:10/9/2017)... Texas (PRWEB) , ... October ... ... study published on October 5, 2017, in the medical journal, Epilepsia, Brain ... with the gold standard, video EEG, in detecting generalized tonic-clonic seizures (GTCS) ...
(Date:10/7/2017)... ... October 06, 2017 , ... ... ProxiMeta™ Hi-C metagenome deconvolution product, featuring the first commercially available Hi-C kit. ... to perform Hi-C metagenome deconvolution using their own facilities, supplementing the company’s ...
(Date:10/6/2017)... ... 2017 , ... The HealthTech Venture Network (HTVN) is proud ... annual Conference where founders, investors, innovative practitioners and collaborators are invited to a ... early stage digital health and med tech companies. , This day-long event will ...
Breaking Biology Technology: