Navigation Links
New manual from Cold Spring Harbor Laboratory Press on culturing neural cells
Date:10/25/2013

Cell culture systems for specific neural cell types are essential for studies of their development and function.

Purifying and Culturing Neural Cells: A Laboratory Manual provides step-by-step protocols for isolating specific cell populations from rodent tissues and culturing them under conditions that closely resemble those in vivo. The contributors describe in detail how to dissect the brain, spinal cord, and other tissues; how to separate cells using mechanical and enzymatic tissue-dissociation strategies; the use of immunopanning and fluorescence-activated cell sorting (FACS) to enrich the target cell population; and the culture conditions that optimize cell viability and growth. Retinal ganglion cells, motor neurons, dorsal root ganglion cells, astrocytes, oligodendrocytes, and Schwann cells are covered, as are vascular cells such as pericytes and endothelial cells. Myelinating co-cultures of neurons and oligodendrocytes are also described.

The manual includes detailed recipes for media and reagents, tips for avoiding common pitfalls, and advice for designing new immunopanning protocols using tissues from other sources. Many of the protocols are accompanied by freely accessible online movies that demonstrate critical steps of the procedures. This is an essential laboratory companion for all neurobiologists, from the graduate student level upwards.


'/>"/>

Contact: Robert Redmond
rredmond@cshl.edu
516-422-4101
Cold Spring Harbor Laboratory
Source:Eurekalert  

Page: 1

Related biology news :

1. The most celebrated lab manual in molecular biology has been updated and expanded
2. Mid-Atlantic suburbs can expect an early spring thanks to the heat of the big city
3. Annual Drug Discovery Conferences Being Held in Boston MA, Spring 2012
4. An early spring drives butterfly population declines
5. Why spring is blooming marvelous (and climate change makes it earlier)
6. First fruitful, then futile: Ammonites or the boon and bane of many offspring
7. Springer and the Beijing Normal University Press sign agreement on new book series
8. Springer will collaborate with the Italian Society for Ultrasound in Medicine and Biology
9. Genetics Society of America announces Spring 2013 DeLill Nasser Travel Award recipients
10. Fetal exposure to PVC plastic chemical linked to obesity in offspring
11. In the Eastern US, spring flowers keep pace with warming climate
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
New manual from Cold Spring Harbor Laboratory Press on culturing neural cells
(Date:5/23/2017)... 2017  Hunova, the first robotic gym for the rehabilitation and functional ... in Genoa, Italy . The first 30 robots will ... USA . The technology was developed and patented at ... IIT spin-off Movendo Technology thanks to a 10 million euro investment from ... click: ...
(Date:5/16/2017)... 2017   Bridge Patient Portal , an ... MD EMR Systems , an electronic medical record ... have established a partnership to build an interface ... GE Centricity™ products, including Centricity Practice Solution (CPS), ... These new integrations will allow healthcare delivery networks ...
(Date:4/24/2017)... , April 24, 2017 ... and partner with  Identity Strategy Partners, LLP (IdSP) ... "With or without President Trump,s March 6, 2017 ... Terrorist Entry , refugee vetting can be instilled with ... resettlement. (Right now, all refugee applications are suspended ...
Breaking Biology News(10 mins):
(Date:10/10/2017)... ... 2017 , ... For the second time in three years, ... Award. Representatives of the FirstHand program travelled to Washington, D.C. Tuesday, October 10th, ... mission is to change the trajectory of STEM education in America by dramatically ...
(Date:10/10/2017)... ... October 10, 2017 , ... ... business process optimization firm for the life sciences and healthcare industries, announces a ... in San Francisco. , The presentation, “Automating GxP Validation for Agile Cloud Platforms,” ...
(Date:10/9/2017)... ... 09, 2017 , ... At its national board meeting in North Carolina, ... University’s Departments of Physics and Astronomy, has been selected for membership in ARCS ... for the 2015 Breakthrough Prize in Fundamental physics for the discovery of the accelerating ...
(Date:10/7/2017)... ... October 06, 2017 , ... ... its ProxiMeta™ Hi-C metagenome deconvolution product, featuring the first commercially available Hi-C ... software to perform Hi-C metagenome deconvolution using their own facilities, supplementing the ...
Breaking Biology Technology: