Navigation Links
New lab manual focuses on essential methods for purifying and characterizing proteins
Date:10/7/2008

COLD SPRING HARBOR, N.Y. (Oct. 7, 2008) A new, user-friendly laboratory manual for protein purification and analysis has just been released by Cold Spring Harbor Laboratory Press. Designed for routine, day-to-day use in the laboratory, it includes essential step-wise protocols as well as background information, recommended experimental strategies, and troubleshooting advice on the most fundamental protein-related methods used by scientists at all levels.

Understanding how proteins function is an essential part of many biological research endeavors. The complexity and sheer number of proteins in a cell are impediments to identifying proteins of interest or purifying proteins for function and structure analysis. Thus, reducing the complexity of a protein sample or in some cases purifying a protein to homogeneity is necessary.

The convenient new manual, Basic Methods in Protein Purification and Analysis, integrates established in vitro and in vivo molecular techniques with more modern in silico methods. It takes the user from the initial steps of obtaining cellular and subcellular extracts, through the purification and isolation steps appropriate for the protein of interest, and, finally, to the steps involved in characterizing and identifying proteins, protein complexes, and protein-protein interactions. Rounding out the manual is an extensive appendix of essential methods for quantifying protein concentration, stabilizing and storing proteins, concentrating proteins, and immunoblotting.

Basic Methods in Protein Purification and Analysis was derived in large part from the popular laboratory manuals Proteins and Proteomics, Purifying Proteins for Proteomics (both by Richard Simpson), and Protein-Protein Interactions (edited by Erica Golemis and Peter Adams), which have proven to be both very successful and highly regarded.

This is the second volume in the Basic Methods series; the first, Basic Methods in Microscopy (http://www.cshlpress.com/link/basmicp.htm) focused on essential techniques for imaging cells and tissues, including sample preparation, microscope use, and image interpretation.


'/>"/>

Contact: Ingrid Benirschke
benirsch@cshl.edu
619-275-6021
Cold Spring Harbor Laboratory
Source:Eurekalert  

Related biology news :

1. Project focuses on production of hydrogen from bacteria and sunlight
2. Rethinking who should be considered essential during a pandemic flu outbreak
3. Researchers find an essential gene for forming ears of corn
4. New study reveals large scale conservation essential
5. Substantial improvement in essential cheap solar cell process
6. Trainor Lab characterizes gene essential for prenatal development of nervous system
7. Earth Observation essential for geohazard mitigation
8. ESA to provide essential launch control services to EUMETSAT
9. How did chemical constituents essential to life arise on primitive Earth?
10. Study shows vitamin C is essential for plant growth
11. Radioactivity: Discover the lowest amounts with new methods
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
New lab manual focuses on essential methods for purifying and characterizing proteins
(Date:3/21/2016)... 2016 Unique technology combines ... superior security   Xura, Inc. ... secure digital communications services, today announced it is working ... enterprise customers, particularly those in the Financial Services Sector, ... authentication within a mobile app, alongside, and in combination ...
(Date:3/14/2016)... Florida , March 14, 2016 ... the growing mobile commerce market, announces the airing of a ... channels starting the week of March 21 st .  The ... CNBC, including its popular Squawk on the Street show. ... focused on the growing mobile commerce market, announces the airing ...
(Date:3/10/2016)... , March 10, 2016   Unisys Corporation (NYSE: ... Border Protection (CBP) is testing its biometric identity solution ... Diego to help identify certain non-U.S. citizens leaving ... The test, designed to help determine the efficiency and accuracy ... in February and will run until May 2016. --> ...
Breaking Biology News(10 mins):
(Date:4/28/2016)... ... April 28, 2016 , ... Next week on May 5 ... first-in-class technologies for tissue stem cell counting and expansion to gene-editing scientists and ... & CRISPR-based Genome Engineering in Burlington, Massachusetts. , The attention of most gene-editing ...
(Date:4/27/2016)... , ... April 27, 2016 , ... The Pittcon Organizing ... 2019. Chuck has been a volunteer member of Committee since 1987. Since then, ... board of directors and treasurer and was chairman for both the program and exposition ...
(Date:4/27/2016)... , ... April 27, 2016 , ... ... Jon Clark has joined the company as an Expert Consultant. Mr. Clark ... industry collaborations and managing the development of small molecule monographs based on analytical ...
(Date:4/27/2016)... , April 27, 2016 MedDay, ... disorders, today announces the appointment of Catherine Moukheibir as Chairman ... MedDay,s previous Chairman, Jean Jacques Garaud , who contributed ... change is effective immediately. Catherine started her career ... and London .  She held ...
Breaking Biology Technology: