Navigation Links
New lab manual focuses on essential methods for purifying and characterizing proteins
Date:10/7/2008

COLD SPRING HARBOR, N.Y. (Oct. 7, 2008) A new, user-friendly laboratory manual for protein purification and analysis has just been released by Cold Spring Harbor Laboratory Press. Designed for routine, day-to-day use in the laboratory, it includes essential step-wise protocols as well as background information, recommended experimental strategies, and troubleshooting advice on the most fundamental protein-related methods used by scientists at all levels.

Understanding how proteins function is an essential part of many biological research endeavors. The complexity and sheer number of proteins in a cell are impediments to identifying proteins of interest or purifying proteins for function and structure analysis. Thus, reducing the complexity of a protein sample or in some cases purifying a protein to homogeneity is necessary.

The convenient new manual, Basic Methods in Protein Purification and Analysis, integrates established in vitro and in vivo molecular techniques with more modern in silico methods. It takes the user from the initial steps of obtaining cellular and subcellular extracts, through the purification and isolation steps appropriate for the protein of interest, and, finally, to the steps involved in characterizing and identifying proteins, protein complexes, and protein-protein interactions. Rounding out the manual is an extensive appendix of essential methods for quantifying protein concentration, stabilizing and storing proteins, concentrating proteins, and immunoblotting.

Basic Methods in Protein Purification and Analysis was derived in large part from the popular laboratory manuals Proteins and Proteomics, Purifying Proteins for Proteomics (both by Richard Simpson), and Protein-Protein Interactions (edited by Erica Golemis and Peter Adams), which have proven to be both very successful and highly regarded.

This is the second volume in the Basic Methods series; the first, Basic Methods in Microscopy (http://www.cshlpress.com/link/basmicp.htm) focused on essential techniques for imaging cells and tissues, including sample preparation, microscope use, and image interpretation.


'/>"/>

Contact: Ingrid Benirschke
benirsch@cshl.edu
619-275-6021
Cold Spring Harbor Laboratory
Source:Eurekalert  

Related biology news :

1. Project focuses on production of hydrogen from bacteria and sunlight
2. Rethinking who should be considered essential during a pandemic flu outbreak
3. Researchers find an essential gene for forming ears of corn
4. New study reveals large scale conservation essential
5. Substantial improvement in essential cheap solar cell process
6. Trainor Lab characterizes gene essential for prenatal development of nervous system
7. Earth Observation essential for geohazard mitigation
8. ESA to provide essential launch control services to EUMETSAT
9. How did chemical constituents essential to life arise on primitive Earth?
10. Study shows vitamin C is essential for plant growth
11. Radioactivity: Discover the lowest amounts with new methods
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
New lab manual focuses on essential methods for purifying and characterizing proteins
(Date:6/2/2016)... 2016   The Weather Company , an IBM Business ... industry-first capability in which consumers will be able to interact ... questions via voice or text and receive relevant information about ... Marketers have long sought an advertising solution that can ... personal, relevant and valuable; and can scale across millions of ...
(Date:5/20/2016)... MINNEAPOLIS , May 20, 2016  VoiceIt ... technology partnership with VoicePass. By working ... user experience.  Because VoiceIt and VoicePass take slightly ... two engines increases both security and usability. ... expressed excitement about this new partnership. ...
(Date:5/3/2016)... , May 3, 2016  Neurotechnology, a ... the MegaMatcher Automated Biometric Identification System (ABIS) ... large-scale multi-biometric projects. MegaMatcher ABIS can process multiple ... using any combination of fingerprint, face or iris ... MegaMatcher SDK and MegaMatcher Accelerator , ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... ... , ... Supplyframe, the Industry Network for electronics hardware design ... Located in Pasadena, Calif., the Design Lab’s mission is to bring together inventors ... and brought to market. , The Design Lab is Supplyframe’s physical representation of ...
(Date:6/23/2016)... 23, 2016 Apellis Pharmaceuticals, Inc. today ... trials of its complement C3 inhibitor, APL-2. The ... ascending dose studies designed to assess the safety, ... injection in healthy adult volunteers. Forty ... a single dose (ranging from 45 to 1,440mg) ...
(Date:6/23/2016)... -- On Wednesday, June 22, 2016, the NASDAQ ... Dow Jones Industrial Average edged 0.27% lower to finish at ... Stock-Callers.com has initiated coverage on the following equities: Infinity Pharmaceuticals ... NKTR ), Aralez Pharmaceuticals Inc. (NASDAQ: ARLZ ... Learn more about these stocks by accessing their free trade ...
(Date:6/23/2016)... ... 2016 , ... Velocity Products, a division of Morris Group, ... exclusively for Okuma CNC machining centers at The International Manufacturing Technology Show, IMTS, ... companies with expertise in toolholding, cutting tools, machining dynamics and distribution, Velocity SMART ...
Breaking Biology Technology: