Navigation Links
New lab manual focuses on essential methods for purifying and characterizing proteins
Date:10/7/2008

COLD SPRING HARBOR, N.Y. (Oct. 7, 2008) A new, user-friendly laboratory manual for protein purification and analysis has just been released by Cold Spring Harbor Laboratory Press. Designed for routine, day-to-day use in the laboratory, it includes essential step-wise protocols as well as background information, recommended experimental strategies, and troubleshooting advice on the most fundamental protein-related methods used by scientists at all levels.

Understanding how proteins function is an essential part of many biological research endeavors. The complexity and sheer number of proteins in a cell are impediments to identifying proteins of interest or purifying proteins for function and structure analysis. Thus, reducing the complexity of a protein sample or in some cases purifying a protein to homogeneity is necessary.

The convenient new manual, Basic Methods in Protein Purification and Analysis, integrates established in vitro and in vivo molecular techniques with more modern in silico methods. It takes the user from the initial steps of obtaining cellular and subcellular extracts, through the purification and isolation steps appropriate for the protein of interest, and, finally, to the steps involved in characterizing and identifying proteins, protein complexes, and protein-protein interactions. Rounding out the manual is an extensive appendix of essential methods for quantifying protein concentration, stabilizing and storing proteins, concentrating proteins, and immunoblotting.

Basic Methods in Protein Purification and Analysis was derived in large part from the popular laboratory manuals Proteins and Proteomics, Purifying Proteins for Proteomics (both by Richard Simpson), and Protein-Protein Interactions (edited by Erica Golemis and Peter Adams), which have proven to be both very successful and highly regarded.

This is the second volume in the Basic Methods series;
'/>"/>

Contact: Ingrid Benirschke
benirsch@cshl.edu
619-275-6021
Cold Spring Harbor Laboratory
Source:Eurekalert  

1

GOOD

Page: 1 2

Related biology news :

1. Project focuses on production of hydrogen from bacteria and sunlight
2. Rethinking who should be considered essential during a pandemic flu outbreak
3. Researchers find an essential gene for forming ears of corn
4. New study reveals large scale conservation essential
5. Substantial improvement in essential cheap solar cell process
6. Trainor Lab characterizes gene essential for prenatal development of nervous system
7. Earth Observation essential for geohazard mitigation
8. ESA to provide essential launch control services to EUMETSAT
9. How did chemical constituents essential to life arise on primitive Earth?
10. Study shows vitamin C is essential for plant growth
11. Radioactivity: Discover the lowest amounts with new methods
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
New lab manual focuses on essential methods for purifying and characterizing proteins
(Date:6/19/2013)... of Pittsburgh has developed antibacterial compounds, derived from ... potential treatments for drug-resistant bacterial infections and appear ... quite small, making them inexpensive and easy to ... 2013 issue of the journal Antimicrobial Agents ... probable applications will likely be the chronic bacterial ...
(Date:6/19/2013)... , June 19, 2013 MedNet ... in clinical study management systems, is pleased to announce ... be held June 23-27 in Boston ... MedNet, a recognized leader in cloud-based eClinical solutions, has ... will be showcasing iMedNet™ EDC , ...
(Date:6/19/2013)... is the most potent physiological anabolic agent for ... synthesis of lipids, protein and carbohydrates, and inhibiting ... It also plays a major role in stimulating ... is metabolized and removed from the blood following ... molecular mechanisms by which insulin regulates glucose uptake ...
Breaking Biology News(10 mins):HIV-derived antibacterial shows promise against drug-resistant bacteria 2MedNet Solutions To Spotlight iMedNet EDC At The 49th DIA Annual Meeting 2Breakthrough research of essential molecule reveals important targets in diabetes and obesity 2
... and likely to die of - heart disease compared ... are to blame, according to a new University of Leicester ... Maciej Tomaszewski, New Blood Lecturer in Cardiovascular Medicine in the ... that this "male disadvantage" may be related to the sex-specific ...
... an agreement was reached by the Alliance for a ... sales restrictions on "competitive foods" such as soft drinks ... say limiting the availability of soft drinks at school ... elementary school children. In a study of more ...
... Tech have used skin cells to create artificial bones ... into other tissues such as tendons or ligaments. The ... softer tissue rather than the sudden shift of previously ... and allowing them to handle weight more successfully. The ...
Cached Biology News:Sex hormones link to heart risk 2Engineers create bone that blends into tendons 2
(Date:6/19/2013)... , June 19, 2013 /PRNewswire/ ... Bureau presents Innovative Drug Delivery ... Technology Licensing Opportunities for Generic ... There are more ... release drugs approved all over ...
(Date:6/19/2013)... India’s vast and growing population means ... up to a billion dollars per year by ... taking serious action to better regulate and oversee ... will examine:, ,     Recent changes in ... long term impacts ,     Foreseeable opportunities ...
(Date:6/19/2013)... 18, 2013 A new look at ... and other trends will highlight technical sessions at ... fall. In its 33rd year, the three-day event is ... of key suppliers, approximately 100 technical presentations and numerous ... international society for optics and photonics , the meeting ...
(Date:6/19/2013)... Adding to their already extensive supply ... Simport’s Dropette® and Heathrow Scientific disposable plastic ... basic biology, chemistry and any type of liquid handling ... 35 years, Simport has been supplying the science community ... the Simport Dropette®. Simport’s Dropette® is a one-piece ...
Breaking Biology Technology:Innovative Drug Delivery Systems: Novel Product and Formulation Technology Licensing Opportunities for Generic Pharmaceutical Companies 2Innovative Drug Delivery Systems: Novel Product and Formulation Technology Licensing Opportunities for Generic Pharmaceutical Companies 3‘Big Glass’ and Visions for the Future are on the Program for SPIE Photomask Technology 2‘Big Glass’ and Visions for the Future are on the Program for SPIE Photomask Technology 3Leading Pipette Distributor Pipette.com Now Stocks Transfer Pipettes: Simport’s Dropette and Heathrow Scientific Disposable Plastic Transfer Pipettes 2
... RUTHERFORD, N.J. , Feb. 10 Cambrex Corporation (NYSE: ... December 31, 2009 . , Highlights , ... sales decreased 15.8% compared to fourth quarter 2008.  For the full year ... foreign currency. EBITDA was $9.4 million in the fourth ...
... , , ... Oramed,Pharmaceuticals, Inc. (OTCBB: ORMP.OB) ( http://www.oramed.com ), a developer,of alternative drug ... , Ph.D., was chosen to lecture at the Third,International Conference on ... Basel, Switzerland on February 11, 2010 . Dr. Kidron,will ...
... , PLANTATION, Fla. and ... Biotechnology Inc., a full service Contract Biomanufacturing Company ... agents, jointly announced today their collaborative agreement on ... customer projects in Nuclear Medicine involving Chelation, Bioconjugation ...
Cached Biology Technology:Cambrex Reports Fourth Quarter and Full Year 2009 Results 2Cambrex Reports Fourth Quarter and Full Year 2009 Results 3Cambrex Reports Fourth Quarter and Full Year 2009 Results 4Cambrex Reports Fourth Quarter and Full Year 2009 Results 5Cambrex Reports Fourth Quarter and Full Year 2009 Results 6Cambrex Reports Fourth Quarter and Full Year 2009 Results 7Cambrex Reports Fourth Quarter and Full Year 2009 Results 8Cambrex Reports Fourth Quarter and Full Year 2009 Results 9Cambrex Reports Fourth Quarter and Full Year 2009 Results 10Cambrex Reports Fourth Quarter and Full Year 2009 Results 11Cambrex Reports Fourth Quarter and Full Year 2009 Results 12Cambrex Reports Fourth Quarter and Full Year 2009 Results 13Cambrex Reports Fourth Quarter and Full Year 2009 Results 14Cambrex Reports Fourth Quarter and Full Year 2009 Results 15Oramed's Chief Scientific Officer, Miriam Kidron, Ph.D., Chosen to Lecture at the Third International Conference on Advanced Technologies and Treatments for Diabetes in Basel, Switzerland 2Oramed's Chief Scientific Officer, Miriam Kidron, Ph.D., Chosen to Lecture at the Third International Conference on Advanced Technologies and Treatments for Diabetes in Basel, Switzerland 3Goodwin Biotechnology Inc. and Macrocyclics Announce Collaboration 2
UNIVERSAL SCORECARD DNA, 1 EA. * Category: Reagents/Consumables....
mitochondrial proteins (human heart) for 2-D gel electrophoresis *5 mg/mL*...
... GeneMaths XT is undoubtedly the most complete ... advanced concept of layers and subsets makes ... layers for different data outputs, for example ... etc., as well as different subsets of ...
...
Biology Products: