Navigation Links
New key to organism complexity identified
Date:1/17/2013

l of protein subunits and domains. Nogales and her colleagues are the first group to obtain three-dimensional visualization of human TFIID that is bound to DNA. The single-particle cryo-electron microscopy technique they employed records a series of two-dimensional images of an individual molecules or macromolecular complexes frozen in random orientations, then computationally combines these images into high-resolution 3D reconstructions.

"Through cryo-EM and extensive image-sorting, we found that TFIID exhibits a surprising degree of flexibility, moving its lobe A, a region that covers approximately one-third of the complex, by 100 angstroms across its central channel," says Cianfrocco, lead author of the Cell paper. "This movement of the lobe A is absolutely essential for TFIID to bind to DNA."

Nogales says that while many macromolecular complexes are known to be flexible, this typically involves the limited movement of a small region within the complex, or some tiny motion of the entire complex. The movement of TFIID's lobe A represents an entire restructuring that dramatically alters what the molecule can do. In the canonical state, TFIID's lobe A is bound to its lobe C, which appears to be the preferred form of free TFIID. In the rearranged state, TFIID's lobe A is bound to its lobe B, which is the state in which it can then strongly bind to DNA promoters.

"The TFIIA molecule serves as the mediator for this transition, maintaining TFIID in the canonical state in the absence of DNA and initiating the formation of the rearranged state in the presence of promoter DNA," Cianfrocco says. "Without the presence of TFIIA, the binding of TFIID to DNA is very weak."

Nogales and her colleagues are now studying how TFIID, once it is bound to DNA, recruits the rest of the machinery required to transcribe the genetic message into RNA.

"Our new work will involve constructing a macromolecular complex that is well over two
'/>"/>

Contact: Lynn Yarris
lcyarris@lbl.gov
510-486-5375
DOE/Lawrence Berkeley National Laboratory
Source:Eurekalert  

Page: 1 2 3 4

Related biology news :

1. Production of 5-aminovaleric and glutaric acid by metabolically engineered microorganism
2. Keeping ship hulls free of marine organisms
3. Hearty organisms discovered in bitter-cold Antarctic brine
4. Strange diet for methane consuming microorganisms
5. Plants recognise pathogenic and beneficial microorganisms
6. Small marine organisms big changes could affect world climate
7. Small organisms could dramatically impact worlds climate
8. Unusual symbiosis discovered in marine microorganisms
9. New research suggests bacteria are social microorganisms
10. Why do organisms build tissues they seemingly never use?
11. Progress of arachidonic acid biosynthesis in microorganisms
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
New key to organism complexity identified
(Date:9/18/2014)... have built the first smartphone app that automatically ... trends. In other words, your smartphone knows your ... -- and how that affects you. , The ... and loneliness to their academic performance, also may ... example, to monitor mental health, trigger intervention and ...
(Date:9/18/2014)... 18, 2014Many Americans across racial and ethnic groups ... impact on their day-to-day life, more so than ... and speech (57% of African-Americans, 49% of non-Hispanic ... When asked which disease or ailment is the ... first among African-Americans followed by AIDS/HIV. Hispanics ...
(Date:9/18/2014)... celebrated tonight at the third annual Golden Goose Award ... premature infants and in paving the way for the ... supported by the National Science Foundation, the National Institutes ... be honored at a ceremony at the Library of ... of Congress will be on hand to help present ...
Breaking Biology News(10 mins):New Dartmouth smartphone app reveals users' mental health, performance, behavior 2New Dartmouth smartphone app reveals users' mental health, performance, behavior 3New Dartmouth smartphone app reveals users' mental health, performance, behavior 4Americans rate losing eyesight as having greatest impact on their lives 2Americans rate losing eyesight as having greatest impact on their lives 33rd annual Golden Goose award ceremony honors 8 researchers; Unusual work had big results 2
... NEW YORK (APRIL 15, 2008) A comprehensive book of ... was released today by the Wildlife Conservation Society. ... on such issues as diseases shared by animals and people; ... as Afghanistan. State of the Wild: A Global Portrait ...
... research demonstrates that novel probe technology based ... force microscopy (AFM) cantilevers for applications such ... and single molecule mechanics measurements. In ... these novel probes simultaneously measure material properties ...
... with high cholesterol in their early 40s are more ... cholesterol, according to research that will be presented at ... Meeting in Chicago, April 1219, 2008. "Our findings ... patients to attack high cholesterol levels in their,40s to ...
Cached Biology News:Wildlife Conservation Society releases definitive 'state of the wild' 2Fast AFM probes measure multiple properties of biomolecules or materials simultaneously 2Fast AFM probes measure multiple properties of biomolecules or materials simultaneously 3
(Date:9/18/2014)... HARBOR, Mich. , Sept. 18, 2014  Health Enhancement ... biotech firm investigating early-stage algae bioactive compounds and metabolic processes, ... to the company,s board of directors. Ms. Nola E. ... and investment advisor joined the board effective September 17, 2014. ... Ms. Masterson agree to join our board," states Andrew ...
(Date:9/18/2014)... Texas Governor Rick Perry, Texas ... Health Science Center CEO Brett Giroir, M.D., and ... Human Services (HHS), State of Texas and biopharmaceutical ... vaccine manufacturing facility in Bryan, Texas, which when ... Texas A&M Biocorridor – a rapidly evolving hub ...
(Date:9/18/2014)... 2014 Research and Markets has ... Stem Cell Industry Report, 2014-2017" report to their ... cells that can differentiate into specialized cells and can ... cell therapy can be applied to treatment of cardiovascular ... system diseases, damage or lesion of liver, kidney and ...
(Date:9/18/2014)... Sept. 18, 2014 Research and Markets ... Human Albumin Industry Report 2014" report to their ... Report 2014 is a professional and in-depth study on ... The report provides a basic overview of ... structure. The human albumin market analysis is provided for ...
Breaking Biology Technology:Health Enhancement Announces New Board Member In Advance Of Annual Shareholder Meeting 2Texas A&M Dedicates National Pandemic Influenza Vaccine Manufacturing Facility, on Track for 2016 Start-Up Phase 2Texas A&M Dedicates National Pandemic Influenza Vaccine Manufacturing Facility, on Track for 2016 Start-Up Phase 3Texas A&M Dedicates National Pandemic Influenza Vaccine Manufacturing Facility, on Track for 2016 Start-Up Phase 4Global and Chinese Stem Cell Industry Report, 2014-2017 2Global and Chinese Stem Cell Industry Report, 2014-2017 3Global Human Albumin Industry Report 2014 2Global Human Albumin Industry Report 2014 3
... Institute for Clinical Research, Inc. (PICR) today announced that president and CEO Dr. ... Year ® 2010 Award in San Diego for the second year ... June 9, 2010 at the Hyatt La Jolla Aventine. , ... Dr. Hompesch is being recognized for his establishment and leadership of PICR, ...
... 12 The International Collaborative Cancer Group, in collaboration with the ... Intergroup Exemestane Study (IES).  IES is a randomized, double blind multinational trial ... period of 91 months. Max Neeman will be responsible for ... , , ...
... ... event for experts in detection and identification of biological and chemical threats and will ... identification, and analysis both in the field and at the point-of-care. , ... (PRWEB) May 12, 2010 -- ...
Cached Biology Technology:Profil Institute's Dr. Marcus Hompesch Named Finalist in Ernst & Young Entrepreneur Of The Year(R) 2010 Award for Second Year in a Row 2Profil Institute's Dr. Marcus Hompesch Named Finalist in Ernst & Young Entrepreneur Of The Year(R) 2010 Award for Second Year in a Row 3Max Neeman International Signs Agreement for Data Management/EDC of Large Phase IV Trials from Multiple Sites 2U.S. Department of Homeland Security Keynote to Open 16th Biodetection Technologies Conference Next Month in Arlington, VA 2U.S. Department of Homeland Security Keynote to Open 16th Biodetection Technologies Conference Next Month in Arlington, VA 3
The Holten mini vertical flow workbench provides a particle-free environment for sample protection against airborne contaminants....
...
Medium bottle for hybridization 250 x 35mm...
Request Info...
Biology Products: