Navigation Links
New insights into DNA repair process may spur better cancer therapies
Date:9/30/2013

DURHAM, N.C. By detailing a process required for repairing DNA breakage, scientists at the Duke Cancer Institute have gained a better understanding of how cells deal with the barrage of damage that can contribute to cancer and other diseases.

The insights, reported online the week of Sept. 30, 2013, in the journal Proceedings of the National Academy of Sciences, build on earlier work by the research team and identify new prospects for developing cancer therapies.

The researchers have focused on a complex series of events that cells routinely undertake to repair DNA damaged by sun exposure, smoking and even normal metabolism. If not correctly repaired, DNA breakages can result in cellular damage leading to cancer.

"We never had good assays to measure how DNA breaks are repaired, and there were few good tools to study how that repair unfolds at the molecular level," said senior author Michael Kastan, M.D., PhD, executive director of the Duke Cancer Institute. "Our work for the first time enables us to both sensitively measure the repair of DNA breaks and study the molecular mechanisms by which they occur."

DNA inside the cell faces a challenge for repairing itself because it is so compacted in the cell nucleus. Tightly wrapped in a complex of proteins called chromatin, the DNA is spooled like thread around a protein structure called a nucleosome. DNA could suffer a breakage that would go unheeded if it remained deep within the reel.

The system developed by Kastan and colleagues induced DNA breakage at defined points on the DNA strands, enabling researchers to chronicle events as the cells launched the repair process.

What they described for the first time was a choreographed interaction in which the tightly wound DNA was temporarily loosened when a key protein, called nucleolin, was recruited to the breakage site, disrupting the nucleosome spool. The process was then reversed when the nucleosome was re-formed after repair was complete.

"Our study demonstrates for the first time the functional importance of nucleosome disruption in DNA repair," Kastan said. "This nucleosome disruption allows DNA repair proteins to access the DNA lesion and begin the process of mending the breakage."

Kastan said the finding provides key insights for how cells remain healthy, as well as how the repair process could potentially be manipulated. New cancer therapies, for instance, could target nucleolin to enhance sensitivity of tumor cells to radiation or chemotherapies, he said.

"This could give us an opportunity to make current treatments more potent," Kastan said. "That would be a next area of research, which we are especially interested in pursuing."


'/>"/>

Contact: Sarah Avery
sarah.avery@duke.edu
919-660-1306
Duke University Medical Center
Source:Eurekalert

Related biology news :

1. Erratic proteins: New insights into a transport mechanism
2. Eyewear Market 2014 Insights and System Refresher Report
3. Global analysis reveals new insights into the ribosome -- with important implications for disease
4. Potential diagnostic marker for zinc status offers insights into the effects of zinc deficiency
5. New insights into neuroblastoma tumor suppressor may provide clues for improved treatment
6. UCLA life scientists present new insights on climate change and species interactions
7. Insights into deadly coral bleaching could help preserve reefs
8. New insights into how genes turn on and off
9. New insights into the development of the heart
10. Peach genome offers insights into breeding strategies for biofuels crops
11. Novel insights into the evolution of protein networks
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/8/2017)... AWRE ), a leading supplier of biometrics software ... year ended December 31, 2016. Revenue for ... $6.9 million in the same quarter last year. Operating income ... to $2.6 million in the fourth quarter of 2015. Net ... or $0.02 per diluted share, which compares to $1.8 million, ...
(Date:2/8/2017)... 7, 2017 The biometrics market has ... confluence of organizations, desires to better authenticate or ... (password and challenge questions), biometrics is quickly working ... The market is driven by use cases, though ... and enterprise uses cases, with consumer-facing use cases ...
(Date:2/7/2017)...   MedNet Solutions , an innovative SaaS-based eClinical ... research, is pleased to announce that the latest release ... flexible and award winning eClinical solution, is now available ... is a proven Software-as-a-Service (SaaS) clinical research technology platform ... also delivers an entire suite of eClinical tools to ...
Breaking Biology News(10 mins):
(Date:3/22/2017)... York , March 22, 2017 ... largely fragmented, states a research report by Transparency Market ... Pfizer Inc., Amgen Inc., and AbbVie Inc., accounted for ... 2015. The prominent players in this market are focusing ... their product portfolio, which is likely to lead to ...
(Date:3/22/2017)...   Boston Biomedical , an industry leader in ... cancer stemness pathways, today announced its Board of Directors ... Executive Officer, effective April 24, 2017. ... M.D., FACP, who has led Boston Biomedical since he ... Boston Biomedical has grown from a "garage startup" without ...
(Date:3/22/2017)... ... March 21, 2017 , ... Okyanos Cell Therapy has ... part of their live events series, “Stem Cell Therapy: The Next Phase in the ... the 2013 Stem Cell Research and Therapy Act, Okyanos maintains a mission ...
(Date:3/22/2017)... ... March 21, 2017 , ... Aqua Design Innovations ... goal and raising over $30,000 in the first 40 minutes of crowdfunding. EcoQube ... herbs fast, easy, and affordably, anywhere. , “Simply add fertilized water and in ...
Breaking Biology Technology: