Navigation Links
New imaging method sheds light on cell growth
Date:8/25/2011

CHAMPAIGN, Ill. University of Illinois researchers are giving a light answer to the heavy question of cell growth.

Led by electrical and computer engineering professor Gabriel Popescu, the research team developed a new imaging method called spatial light interference microscopy (SLIM) that can measure cell mass using two beams of light. Described in the Proceedings of the National Academy of Sciences, the SLIM technique offers new insight into the much-debated problem of whether cells grow at a constant rate or exponentially.

SLIM is extremely sensitive, quantitatively measuring mass with femtogram accuracy. By comparison, a micron-sized droplet of water weighs 1,000 femtograms. It can measure the growth of a single cell, and even mass transport within the cell. Yet, the technique is broadly applicable.

"A significant advantage over existing methods is that we can measure all types of cells bacteria, mammalian cells, adherent cells, nonadherent cells, single cells and populations," said Mustafa Mir, a graduate student and a first author of the paper. "And all this while maintaining the sensitivity and the quantitative information that we get."

Unlike most other cell-imaging techniques, SLIM a combination of phase-contrast microscopy and holography does not need staining or any other special preparation. Because it is completely non-invasive, the researchers can study cells as they go about their natural functions. It uses white light and can be combined with more traditional microscopy techniques, such as fluorescence, to monitor cells as they grow.

"We were able to combine more traditional methods with our method because this is just an add-on module to a commercial microscope," Mir said. "Biologists can use all their old tricks and just add our module on top."

Because of SLIM's sensitivity, the researchers could monitor cells' growth through different phases of the cell cycle. They found that mammalian cells show clear exponential growth only during the G2 phase of the cell cycle, after the DNA replicates and before the cell divides. This information has great implications not only for basic biology, but also for diagnostics, drug development and tissue engineering.

The researchers hope to apply their new knowledge of cell growth to different disease models. For example, they plan to use SLIM to see how growth varies between normal cells and cancer cells, and the effects of treatments on the growth rate.

Popescu, a member of the Beckman Institute for Advanced Science and Technology at the U. of I., is establishing SLIM as a shared resource on the Illinois campus, hoping to harness its flexibility for basic and clinical research in a number of areas.

"It could be used in many applications in both life sciences and materials science," said Popescu, who also is a professor of physics and of bioengineering. "The interferometric information can translate to the topography of silicon wafers or semiconductors. It's like an iPad we have the hardware, and there are a number of different applications dedicated to specific problems of interest to different labs."


'/>"/>

Contact: Liz Ahlberg
eahlberg@illinois.edu
217-244-1073
University of Illinois at Urbana-Champaign
Source:Eurekalert  

Related biology news :

1. SNM releases new fact sheet on breast cancer and molecular imaging
2. Cheskin Added Value EVP Lee Shupp Discusses Evolving Dynamics of Consumers and Imaging Tech at 6Sight
3. MU brain imaging center provides research for autism, schizophrenia and Parkinsons disease
4. Similarities in imaging the human body, Earths crust focus of conference at UH
5. UNC expands brain imaging study of infants at risk for autism
6. Studies on imaging and tracking transplanted cells
7. Fattysaurus or thinnysaurus? How dinosaurs measure up with laser imaging
8. SNM Symposium on Multimodality Cardiovascular Molecular Imaging
9. Ultrasound imaging now possible with a smartphone
10. First neuroimaging study examining motor execution in children with autism reveals new insights
11. Lyncean Technologies Inc. receives $1.2 M from NCRR to develop new imaging technique
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
New imaging method sheds light on cell growth
(Date:3/29/2017)... , March 29, 2017  higi, the health IT ... North America , today announced a ... the acquisition of EveryMove. The new investment and acquisition ... of tools to transform population health activities through the ... data. higi collects and secures data today ...
(Date:3/27/2017)... , March 27, 2017  Catholic Health Services ... Management Systems Society (HIMSS) Analytics for achieving Stage ... Model sm . In addition, CHS previously earned ... hospitals using an electronic medical record (EMR). ... high level of EMR usage in an outpatient ...
(Date:3/24/2017)... -- Research and Markets has announced the addition of ... - Industry Forecast to 2025" report to their offering. ... The Global Biometric Vehicle ... around 15.1% over the next decade to reach approximately $1,580 million ... estimates and forecasts for all the given segments on global as ...
Breaking Biology News(10 mins):
(Date:10/9/2017)... ... October 09, 2017 , ... ... 2017, in the medical journal, Epilepsia, Brain Sentinel’s SPEAC® System which uses ... EEG, in detecting generalized tonic-clonic seizures (GTCS) using surface electromyography (sEMG). The ...
(Date:10/7/2017)... ... October 06, 2017 , ... ... ProxiMeta™ Hi-C metagenome deconvolution product, featuring the first commercially available Hi-C kit. ... to perform Hi-C metagenome deconvolution using their own facilities, supplementing the company’s ...
(Date:10/6/2017)... ... 06, 2017 , ... The HealthTech Venture Network (HTVN) is ... fourth annual Conference where founders, investors, innovative practitioners and collaborators are invited to ... showcasing early stage digital health and med tech companies. , This day-long event ...
(Date:10/5/2017)... YORBA LINDA, CA (PRWEB) , ... October 05, 2017 , ... ... tech innovators, engineers, and scientists from around the world, is giving back to cancer ... shirt sold in October. , Now through October 31, shoppers can use promo ...
Breaking Biology Technology: