Navigation Links
New genomic technique uncovers coral transcriptome
Date:5/11/2009

Using a new technique for cDNA preparation combined with the latest sequencing methods, researchers have uncovered the larval transcriptome of a reef-building coral (Acropora millepora). Their study, described in the open access journal BMC Genomics, features the most extensive database of genes and genetic markers currently available for any coral.

A collaborative team of researchers from the University of Texas at Austin and Indiana University, led by Mikhail Matz and John Colbourne, developed a new method for transcriptome analysis based on next-generation 454 sequencing technology. De novo transcriptome analysis is a cost-efficient alternative to genome sequencing when the goal is discovery of protein-coding genes and genetic markers. According to Eli Meyer, the primary author on the paper, "Our method specifically addresses problems that had hindered the sequencing of transcriptomes with 454, such as homopolymer (A/T) tracks, adaptor artifacts, and uneven transcript coverage".

The new method requires as little as 1 microgram of total RNA, eliminates the need for a strand-selection step prior to emulsion PCR, and results in 2-5 fold greater 454 sequence output than in any previously reported attempts. Meyer said, "These tools allow for the rapid development of sequence resources for any organism at a low cost a few weeks and a few thousand dollars".

The protocol outlined in the paper was developed for 454-FLX. The current version of the protocol (available from the authors' website) is already modified and tested to work with the latest 'Titanium' version of 454.

As well as demonstrating the technical power of the method, the study has identified approximately 11,000 genes on the basis of sequence similarity with known proteins, over 30,000 markers of genetic variation, and a number of novel candidate genes for stress-related processes. The characterization of the larval transcriptome for this widely s
'/>"/>

Contact: Graeme Baldwin
graeme.baldwin@biomedcentral.com
44-078-774-11853
BioMed Central
Source:Eurekalert  

Page: 1 2

Related biology news :

1. A genomic CluE for cloud computing
2. Concordia University receives more than $22 million for genomics research
3. Rhythmic genomics -- the yeast metronome and the walk of life
4. Major aspergillus genomics supplement published by journal Fungal Genetics and Biology
5. 2009 Signature Genomic Laboratories Travel Award winner announced
6. Cancer Genomics Browser gives cancer researchers a powerful new tool
7. Nutrigenomics -- developing personalized diets for disease prevention -- part 2 just published in OMICS
8. Major NSF grant boosts UNH research on hormonal genomics
9. Genomatix gets patent for comparative genomics method
10. Venter, Somerville and Church highlight DOE/JGI genomics of energy and environment meeting
11. Complete Genomics releases proof-of-concept data for its sequencing technology for the first time
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
New genomic technique uncovers coral transcriptome
(Date:4/17/2014)... way the immune system may fight cancers and viral ... cells to treat illness. , The research, in mice, ... "neighborhood police" specialized squads of defenders that patrol ... entire city, the body. , Scientists at Washington University ... the liver, skin and uterus each has dedicated immune ...
(Date:4/17/2014)... of our ideas about domestication derive from Charles ... by British animal-breeding practices during the 19th century, ... improvement. , It is from Darwin that ... of captive animals from wild species and total ... But animal management in this industrial setting has ...
(Date:4/16/2014)... Florida State University researchers have identified a new ... bone density and muscle mass with obesity. , "It ... were the better your bones would be because the ... Hazel Stiebeling Professor of Nutrition at Florida State. "But, ...
Breaking Biology News(10 mins):Some immune cells defend only 1 organ 2The story of animal domestication retold 2The story of animal domestication retold 3The story of animal domestication retold 4Researchers: Obesity can amplify bone and muscle loss 2
... A new study from Boston University School of Medicine ... (iPS) cells in an experimental model were not rejected ... published online in Cell Stem Cell , demonstrates ... types that could offer treatment for a wide range ...
... Research Institute (TSRI) have been awarded approximately $1.2 million ... the National Institutes of Health to identify, test and ... of diseases, including rheumatoid arthritis, heart disease and several ... director in TSRI,s Translational Research Institute, and Peter S. ...
... German . , The detector combines near-unity ... very low error rate. The results have been published ... and reliable single-photon detectors are among the most sought-after ... yet reached maturity for practical application. Physicist Dr. Wolfram ...
Cached Biology News:BUSM study shows potential of differentiated iPS cells in cell therapy without immune rejection 2Grant to fund development of drug candidates for rheumatoid arthritis, neurodegenerative disorders 2Quantum communication: Each photon counts 2
(Date:1/14/2014)... PA (PRWEB) January 14, 2014 Date: Friday, ... , Location: Warrington Country Club, 1360 Almshouse Road, Warrington, Pa. ... national nonprofit organization solely dedicated to finding a cure for ... affected worldwide, will host its annual Crystal Ball on Friday, ...
(Date:1/14/2014)... , Jan. 14, 2014  3D Communications, a leading provider of strategic ... business, and media events in the United States ... associate Virginia Cox , JD, is returning to the firm,s ... Cox re-joins 3D after more than two years of service ...
(Date:1/14/2014)... 2014 The largest international professional organization of ... therapeutic derivatives thereof has endorsed an educational program ... the challenges of adulterated herb and botanical products. ... The Society for Medicinal Plant and Natural ...
(Date:1/14/2014)... In recent years, growing suspicion about the pharmaceutical ... and promotion has led to unprecedented levels of public ... about the insidious impact of commercialization of research, has ... the world’s biggest pharmas for illegal marketing activities, allegations ...
Breaking Biology Technology:Hepatitis B Foundation to Host Annual Crystal Ball Gala 2Former FDA Associate Commissioner Returns To 3D Communications 2World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 2World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 3World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 4World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 5World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 6World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 7World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 8The Sunshine Act: Necessary Regulation or Unnecessary Dysregulation? New Life Science Webinar Hosted by Xtalks and IRB Services 2The Sunshine Act: Necessary Regulation or Unnecessary Dysregulation? New Life Science Webinar Hosted by Xtalks and IRB Services 3
... fruit fly and roundworm,s functional genomic elements: DNA sequences in ... are turned on and off at various times in different ... Human Genome Project, the genomes of the fruit fly, ... are powerful models for understanding human biology and disease, ...
... 22, 2010 Pathfinder LLC, a private biotechnology company ... cell damage ("Pathfinder"), and SyntheMed, Inc. (OTC Bulletin Board: ... the "Company"), today announced that they have entered into ... renamed  "Pathfinder Cell Therapy, Inc." and the combined company ...
... Inc. (Nasdaq: NVAX ) and scientists at ... Trudy Morrison, published in the January 2011 issue of ... novel virus-like particle (VLP) vaccine candidate against respiratory syncytial ...  This vaccine candidate has been created with technology that ...
Cached Biology Technology:Scientists reveal how biological activity is regulated in fruit fly and roundworm genomes 2Scientists reveal how biological activity is regulated in fruit fly and roundworm genomes 3Scientists reveal how biological activity is regulated in fruit fly and roundworm genomes 4Pathfinder LLC and SyntheMed, Inc. Announce Merger Agreement 2Pathfinder LLC and SyntheMed, Inc. Announce Merger Agreement 3Pathfinder LLC and SyntheMed, Inc. Announce Merger Agreement 4Pathfinder LLC and SyntheMed, Inc. Announce Merger Agreement 5Pathfinder LLC and SyntheMed, Inc. Announce Merger Agreement 6NOVAVAX and University of Massachusetts Medical School Publish Preclinical Safety and Efficacy Study of a Respiratory Syncytial Virus (RSV) Virus-like Particle (VLP) Vaccine Candidate 2NOVAVAX and University of Massachusetts Medical School Publish Preclinical Safety and Efficacy Study of a Respiratory Syncytial Virus (RSV) Virus-like Particle (VLP) Vaccine Candidate 3NOVAVAX and University of Massachusetts Medical School Publish Preclinical Safety and Efficacy Study of a Respiratory Syncytial Virus (RSV) Virus-like Particle (VLP) Vaccine Candidate 4