Navigation Links
New genomic technique uncovers coral transcriptome
Date:5/11/2009

Using a new technique for cDNA preparation combined with the latest sequencing methods, researchers have uncovered the larval transcriptome of a reef-building coral (Acropora millepora). Their study, described in the open access journal BMC Genomics, features the most extensive database of genes and genetic markers currently available for any coral.

A collaborative team of researchers from the University of Texas at Austin and Indiana University, led by Mikhail Matz and John Colbourne, developed a new method for transcriptome analysis based on next-generation 454 sequencing technology. De novo transcriptome analysis is a cost-efficient alternative to genome sequencing when the goal is discovery of protein-coding genes and genetic markers. According to Eli Meyer, the primary author on the paper, "Our method specifically addresses problems that had hindered the sequencing of transcriptomes with 454, such as homopolymer (A/T) tracks, adaptor artifacts, and uneven transcript coverage".

The new method requires as little as 1 microgram of total RNA, eliminates the need for a strand-selection step prior to emulsion PCR, and results in 2-5 fold greater 454 sequence output than in any previously reported attempts. Meyer said, "These tools allow for the rapid development of sequence resources for any organism at a low cost a few weeks and a few thousand dollars".

The protocol outlined in the paper was developed for 454-FLX. The current version of the protocol (available from the authors' website) is already modified and tested to work with the latest 'Titanium' version of 454.

As well as demonstrating the technical power of the method, the study has identified approximately 11,000 genes on the basis of sequence similarity with known proteins, over 30,000 markers of genetic variation, and a number of novel candidate genes for stress-related processes. The characterization of the larval transcriptome for this widely studied coral will enable research into the biological processes underlying stress responses in corals and their evolutionary adaptation to global climate change.


'/>"/>

Contact: Graeme Baldwin
graeme.baldwin@biomedcentral.com
44-078-774-11853
BioMed Central
Source:Eurekalert  

Related biology news :

1. A genomic CluE for cloud computing
2. Concordia University receives more than $22 million for genomics research
3. Rhythmic genomics -- the yeast metronome and the walk of life
4. Major aspergillus genomics supplement published by journal Fungal Genetics and Biology
5. 2009 Signature Genomic Laboratories Travel Award winner announced
6. Cancer Genomics Browser gives cancer researchers a powerful new tool
7. Nutrigenomics -- developing personalized diets for disease prevention -- part 2 just published in OMICS
8. Major NSF grant boosts UNH research on hormonal genomics
9. Genomatix gets patent for comparative genomics method
10. Venter, Somerville and Church highlight DOE/JGI genomics of energy and environment meeting
11. Complete Genomics releases proof-of-concept data for its sequencing technology for the first time
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
New genomic technique uncovers coral transcriptome
(Date:2/9/2016)... Aware, Inc. (NASDAQ: AWRE ), a leading supplier ... fourth quarter and year ended December 31, 2015.  ... of 2015 was $6.9 million, an increase of 61% compared to ... the fourth quarter of 2015 was $2.6 million compared to $0.2 ... --> --> Higher revenue and operating income in ...
(Date:2/4/2016)... The field of Human Microbiome research ... popular hubs of the biotechnology industry. While the ... of human microbiota, have garnered a lot of ... space has literally exploded in terms of both ... focuses on biomedical aspects of research, development, and ...
(Date:2/3/2016)... , Feb. 3, 2016 ... addition of the "Emotion Detection and ... Learning, and Others), Software Tools (Facial Expression, ... End Users,and Regions - Global forecast to ... --> http://www.researchandmarkets.com/research/d8zjcd/emotion_detection ) has announced ...
Breaking Biology News(10 mins):
(Date:2/12/2016)... Feb. 12, 2016 Biocom, the association representing the ... a group of San Diego companies ... its 2016 Precision Medicine Advocacy Fly-In. Biocom Fly-In participants had ... Drug Administration (FDA), the Centers for Medicare and Medicaid Services ... San Diego U.S. Representatives Susan Davis and ...
(Date:2/11/2016)... 2016 Non-profit Consortium Aims to Generate ... Support Research and Discovery --> ... ambitious plan to sequence 100,000 individuals. It is intended to ... least 7 of North and East Asian countries. ... the project will focus on creating phased reference genomes for ...
(Date:2/11/2016)... BioPharma Selling Solutions (Spectra) is a new Contract ... experience, expertise, operational delivery and customer focus to ... in concert with industry leading commercial experts, the ... needs of its clients by providing value-based creative ... non-personal promotion. --> ...
(Date:2/11/2016)... , Feb. 11, 2016   BioInformant announces ... "Stem Cell Research Products, Opportunities, Tools, and Technologies – ... The ... the stem cell industry, BioInformant has more than a ... stem cell market, by stem cell type. This powerful ...
Breaking Biology Technology: