Navigation Links
New genomic technique uncovers coral transcriptome
Date:5/11/2009

Using a new technique for cDNA preparation combined with the latest sequencing methods, researchers have uncovered the larval transcriptome of a reef-building coral (Acropora millepora). Their study, described in the open access journal BMC Genomics, features the most extensive database of genes and genetic markers currently available for any coral.

A collaborative team of researchers from the University of Texas at Austin and Indiana University, led by Mikhail Matz and John Colbourne, developed a new method for transcriptome analysis based on next-generation 454 sequencing technology. De novo transcriptome analysis is a cost-efficient alternative to genome sequencing when the goal is discovery of protein-coding genes and genetic markers. According to Eli Meyer, the primary author on the paper, "Our method specifically addresses problems that had hindered the sequencing of transcriptomes with 454, such as homopolymer (A/T) tracks, adaptor artifacts, and uneven transcript coverage".

The new method requires as little as 1 microgram of total RNA, eliminates the need for a strand-selection step prior to emulsion PCR, and results in 2-5 fold greater 454 sequence output than in any previously reported attempts. Meyer said, "These tools allow for the rapid development of sequence resources for any organism at a low cost a few weeks and a few thousand dollars".

The protocol outlined in the paper was developed for 454-FLX. The current version of the protocol (available from the authors' website) is already modified and tested to work with the latest 'Titanium' version of 454.

As well as demonstrating the technical power of the method, the study has identified approximately 11,000 genes on the basis of sequence similarity with known proteins, over 30,000 markers of genetic variation, and a number of novel candidate genes for stress-related processes. The characterization of the larval transcriptome for this widely studied coral will enable research into the biological processes underlying stress responses in corals and their evolutionary adaptation to global climate change.


'/>"/>

Contact: Graeme Baldwin
graeme.baldwin@biomedcentral.com
44-078-774-11853
BioMed Central
Source:Eurekalert  

Related biology news :

1. A genomic CluE for cloud computing
2. Concordia University receives more than $22 million for genomics research
3. Rhythmic genomics -- the yeast metronome and the walk of life
4. Major aspergillus genomics supplement published by journal Fungal Genetics and Biology
5. 2009 Signature Genomic Laboratories Travel Award winner announced
6. Cancer Genomics Browser gives cancer researchers a powerful new tool
7. Nutrigenomics -- developing personalized diets for disease prevention -- part 2 just published in OMICS
8. Major NSF grant boosts UNH research on hormonal genomics
9. Genomatix gets patent for comparative genomics method
10. Venter, Somerville and Church highlight DOE/JGI genomics of energy and environment meeting
11. Complete Genomics releases proof-of-concept data for its sequencing technology for the first time
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
New genomic technique uncovers coral transcriptome
(Date:4/13/2017)... April 13, 2017 UBM,s Advanced Design and ... will feature emerging and evolving technology through its 3D ... will run alongside the expo portion of the event ... and demonstrations focused on trending topics within 3D printing ... and manufacturing event will take place June 13-15, 2017 at ...
(Date:4/11/2017)... NXT-ID, Inc. (NASDAQ:   NXTD ) ("NXT-ID" or ... independent Directors Mr. Robin D. Richards and Mr. ... the company,s corporate governance and expertise. ... Gino Pereira , Chief Executive Officer said," ... and benefiting from their considerable expertise as we move forward ...
(Date:4/4/2017)... , April 4, 2017   EyeLock LLC ... announced that the United States Patent and Trademark Office ... broadly covers the linking of an iris image with ... transaction) and represents the company,s 45 th issued ... patent is very timely given the multi-modal biometric capabilities ...
Breaking Biology News(10 mins):
(Date:10/12/2017)... (PRWEB) , ... October 12, ... ... ) has launched Rosalind™, the first-ever genomics analysis platform specifically designed for ... complexity. Named in honor of pioneering researcher Rosalind Franklin, who made a ...
(Date:10/11/2017)... CA (PRWEB) , ... October 11, 2017 , ... ... upregulate any gene in its endogenous context, enabling overexpression experiments and avoiding the ... system with small RNA guides is transformative for performing systematic gain-of-function studies. ...
(Date:10/11/2017)... ... October 11, 2017 , ... ComplianceOnline’s Medical Device Summit is back ... 8th June 2018 in San Francisco, CA. The Summit brings together current and former ... CEOs, board directors and government officials from around the world to address key issues ...
(Date:10/11/2017)...  VMS BioMarketing, a leading provider of patient support solutions, ... Educator (CNE) network, which will launch this week. The VMS ... care professionals to enhance the patient care experience by delivering ... health care professionals to help women who have been diagnosed ... ...
Breaking Biology Technology: