Professor Qin Wenbin from BaoTou Medical College first identified the hemoglobin (Hb) A2 phenomenon 30 years ago. His first paper on this phenomenon was published in Acta Biochimica et Biophysica Sinica, in Chinese, in 1981. Subsequent research investigating its mechanism was published in Chinese in the Chinese Biochemical Journal and Progress in Biochemistry and Biophysics in 1991 and more recently in Electrophoresis, in 2010. Using electrophoretic methods, he discovered that Hb is re-released in living red blood cells (RBCs) and demonstrated the significance of this process in theory and in clinical practice. His paper was published in Electrophoresis in 2009. He has since reported a compendium of his findings in a paper entitled "Electrophoresis release of hemoglobin form living red blood cells in Chinese in Scientia Sinica: Life Sciences (2011, issue No. 8) His work has opened up a new field of Hb research.
Most proteomics studies are conducted in a typical manner in which cells are lysed to release protein, after which the intended analyses are conducted. This is particularly true for red blood cells. Lysis of red blood cells releases Hb and the resulting mixture is called the hemolysate. It has long been considered that the analysis of proteins in hemolysate can reveal the true conditions inside the RBCs. However, it was unknown whether Hb obtained from the lysed RBCs had similar properties to Hb in intact living RBCs.
Therefore, in 1981, Professor Qin began comparing intact living RBCs with their hemolysate and found that the electrophoretic position of all Hbs, except for HbA2, were the same in both samples. However, the cause and the mechanism of this phenomenontermed the HbA2 phenomenonwere unknown.
In 1991, to demonstrate the mechanism of the phenomenon he designed two new methods to test the interactions between Hbs, namely (1) Hb cross electrophoresis in starch-agarose mixed gel and (2) Hb bidirectional diagonal electrop
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