Navigation Links
New culture dish could advance human embryonic stem cell research
Date:6/2/2010

ANN ARBOR, Mich.---A new synthetic Petri dish coating could overcome a major challenge to the advancement of human embryonic stem cell research, say University of Michigan researchers.

Under today's regulations, current stem cell lines have limitations in yielding human therapies because the cells have been grown on animal-based substances that don't behave in predictable ways.

"These nondefined, animal-based components create issues with the FDA (the U.S. Food and Drug Administration) and hinder clinical applications," said Joerg Lahann, associate professor of chemical engineering.

Lahann and Gary Smith, an associate professor in obstetrics and gynecology in the U-M Health System, and their co-workers built a new stem cell growth matrix that is completely synthetic and doesn't contaminate the stem cells with foreign substances that could interfere with their normal function.

A paper on the research was published online this week in Nature Biotechnology.

Today's most commonly used matrices are mouse embryonic fibroblast cells and Matrigel, which is made from mouse tumors.

"The problem is that the mouse-derived cells have batch-to-batch variability, and they secrete factors that nobody really understands. Stem cells are very sensitive to their environment," Lahann said.

The unknown factors hamper researchers' attempts to pinpoint how and under what conditions stem cells differentiate---questions paramount to the development of future stem cell therapies.

The team tested six different polymer coatings and found that a water-soluble gel with the acronym PMEDSAH performed well when attached to the Petri dish even after 25 rounds of harvesting stem cells to grow new colonies.

"We have designed a fully synthetic, fully chemically defined hydrogel that has long-term stability and no batch-to-batch variability," Smith said. "Moreover, we have established that it can be used for long-term growth of human embryonic stem cells while maintaining all of their known normal functions.

"These include normal genetic makeup, lack of spontaneous differentiation and maintenance of pluripotency, which means they can still become any cell type of the human body. This is a perfect example of an interdisciplinary collaboration leading to information gained and future discovery of cures and improvements of human health."

Smith is also an associate professor in the departments of Molecular and Integrative Physiology and Urology, as well as director of the Reproductive Sciences Program. Lahann is also an associate professor in the departments of Materials Science and Engineering and Biomedical Engineering.


'/>"/>

Contact: Nicole Casal Moore
ncmoore@umich.edu
734-647-7087
University of Michigan
Source:Eurekalert

Related biology news :

1. Novel 3-D cell culture model shows selective tumor uptake of nanoparticles
2. Emphasizing the precision in precision agriculture
3. Thumb-size microsystem enables cell culture and incubation
4. Agriculture experts meet in Beijing to examine impacts of food prices and climate change on farmers
5. MIT: Culture influences brain function
6. Agriculture is changing the chemistry of the Mississippi River
7. Stevens researchers provide oversight for three-year mariculture program in Egypt and Israel
8. Tomato pathogen genome may offer clues about bacterial evolution at dawn of agriculture
9. Aquaculture concept leaves judges goggle eyed
10. Ancient sunflower fuels debate about agriculture in the Americas
11. New study points to agriculture in frog sexual abnormalities
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/24/2017)... , April 24, 2017 Janice ... partner with  Identity Strategy Partners, LLP (IdSP) , ... or without President Trump,s March 6, 2017 ... Entry , refugee vetting can be instilled with greater ... (Right now, all refugee applications are suspended by ...
(Date:4/13/2017)... According to a new market research report "Consumer IAM Market by ... Service, Authentication Type, Deployment Mode, Vertical, and Region - Global Forecast to ... USD 14.30 Billion in 2017 to USD 31.75 Billion by 2022, at ... ... MarketsandMarkets Logo ...
(Date:4/6/2017)... , April 6, 2017 ... RFID, ANPR, Document Readers, by End-Use (Transportation & Logistics, ... Facility, Oil, Gas & Fossil Generation Facility, Nuclear Power), ... Educational, Other) Are you looking for a ... sector? ...
Breaking Biology News(10 mins):
(Date:10/9/2017)... ... 2017 , ... At its national board meeting in North Carolina, ARCS® ... Departments of Physics and Astronomy, has been selected for membership in ARCS Alumni ... the 2015 Breakthrough Prize in Fundamental physics for the discovery of the accelerating expansion ...
(Date:10/7/2017)... ... October 06, 2017 , ... ... consulting for microscopy and surface analysis, Nanoscience Instruments is now expanding into ... a broad range of contract analysis services for advanced applications. Services will ...
(Date:10/6/2017)... ... October 06, 2017 , ... The HealthTech Venture Network ... at their fourth annual Conference where founders, investors, innovative practitioners and collaborators are ... pitch competition showcasing early stage digital health and med tech companies. , This ...
(Date:10/5/2017)... ... October 05, 2017 , ... Understanding the microbiome, the millions of ... health. Gut Love: You Are My Future, the newest exhibit on display at the ... explores the human condition through the lens of the gut microbiome. , Gut ...
Breaking Biology Technology: