Navigation Links
New culture dish could advance human embryonic stem cell research
Date:6/2/2010

ANN ARBOR, Mich.---A new synthetic Petri dish coating could overcome a major challenge to the advancement of human embryonic stem cell research, say University of Michigan researchers.

Under today's regulations, current stem cell lines have limitations in yielding human therapies because the cells have been grown on animal-based substances that don't behave in predictable ways.

"These nondefined, animal-based components create issues with the FDA (the U.S. Food and Drug Administration) and hinder clinical applications," said Joerg Lahann, associate professor of chemical engineering.

Lahann and Gary Smith, an associate professor in obstetrics and gynecology in the U-M Health System, and their co-workers built a new stem cell growth matrix that is completely synthetic and doesn't contaminate the stem cells with foreign substances that could interfere with their normal function.

A paper on the research was published online this week in Nature Biotechnology.

Today's most commonly used matrices are mouse embryonic fibroblast cells and Matrigel, which is made from mouse tumors.

"The problem is that the mouse-derived cells have batch-to-batch variability, and they secrete factors that nobody really understands. Stem cells are very sensitive to their environment," Lahann said.

The unknown factors hamper researchers' attempts to pinpoint how and under what conditions stem cells differentiate---questions paramount to the development of future stem cell therapies.

The team tested six different polymer coatings and found that a water-soluble gel with the acronym PMEDSAH performed well when attached to the Petri dish even after 25 rounds of harvesting stem cells to grow new colonies.

"We have designed a fully synthetic, fully chemically defined hydrogel that has long-term stability and no batch-to-batch variability," Smith said. "Moreover, we have established that it can be used for long-term growth of human embryonic stem cells while maintaining all of their known normal functions.

"These include normal genetic makeup, lack of spontaneous differentiation and maintenance of pluripotency, which means they can still become any cell type of the human body. This is a perfect example of an interdisciplinary collaboration leading to information gained and future discovery of cures and improvements of human health."

Smith is also an associate professor in the departments of Molecular and Integrative Physiology and Urology, as well as director of the Reproductive Sciences Program. Lahann is also an associate professor in the departments of Materials Science and Engineering and Biomedical Engineering.


'/>"/>

Contact: Nicole Casal Moore
ncmoore@umich.edu
734-647-7087
University of Michigan
Source:Eurekalert

Related biology news :

1. Novel 3-D cell culture model shows selective tumor uptake of nanoparticles
2. Emphasizing the precision in precision agriculture
3. Thumb-size microsystem enables cell culture and incubation
4. Agriculture experts meet in Beijing to examine impacts of food prices and climate change on farmers
5. MIT: Culture influences brain function
6. Agriculture is changing the chemistry of the Mississippi River
7. Stevens researchers provide oversight for three-year mariculture program in Egypt and Israel
8. Tomato pathogen genome may offer clues about bacterial evolution at dawn of agriculture
9. Aquaculture concept leaves judges goggle eyed
10. Ancient sunflower fuels debate about agriculture in the Americas
11. New study points to agriculture in frog sexual abnormalities
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:8/23/2017)... ARMONK, N.Y. , Aug. 23, 2017  The general public,s help ... the human microbiome—the bacteria that live in and on the human body ... ... bacteria in the human microbiome, starting with the gut. The project's goal ... in disease. Photo credit: IBM ...
(Date:6/14/2017)... , June 15, 2017  IBM (NYSE: IBM ) ... international tech event dedicated to developing collaboration between startups and ... on June 15-17. During the event, nine startups will showcase ... value in various industries. France ... the international market, with a 30 percent increase in the ...
(Date:4/24/2017)... -- Janice Kephart , former 9/11 Commission ... LLP (IdSP) , today issues the following statement: ... 6, 2017 Executive Order: Protecting the Nation ... instilled with greater confidence, enabling the reactivation of ... are suspended by until at least July 2017). ...
Breaking Biology News(10 mins):
(Date:10/10/2017)... , ... October 10, 2017 , ... ... Center’s FirstHand program has won a US2020 STEM Mentoring Award. Representatives of the ... for Excellence in Volunteer Experience from US2020. , US2020’s mission is to change ...
(Date:10/10/2017)... ... 2017 , ... The Pittcon Program Committee is pleased to ... who have made outstanding contributions to analytical chemistry and applied spectroscopy. Each award ... conference and exposition for laboratory science, which will be held February 26-March 1, ...
(Date:10/9/2017)... ... October 09, 2017 , ... ... in the medical journal, Epilepsia, Brain Sentinel’s SPEAC® System which uses the ... in detecting generalized tonic-clonic seizures (GTCS) using surface electromyography (sEMG). The prospective ...
(Date:10/6/2017)... ... October 06, 2017 , ... On Tuesday, October 24th, ABC² ... the first-ever adaptive clinical trial for glioblastoma (GBM). The featured speaker will be ... and open to the public, but registration is required. , WHAT: ABC² ...
Breaking Biology Technology: