Navigation Links
New X-ray method shows how frog embryos could help thwart disease
Date:5/17/2013

LEMONT, Ill. An international team of scientists using a new X-ray method recorded the internal structure and cell movement inside a living frog embryo in greater detail than ever before.

This result showcases a new method to advance biological research and the search for new treatments for genetic diseases.

Scientists at Northwestern University and the Karlsruher Institut fr Technologie in Germany, in collaboration with the Advanced Photon Source at the U.S. Department of Energy's Argonne National Laboratory, released the most precise depiction ever of the embryonic development of African clawed frogs, one of the most frequently studied model organisms in biology.

The results titled "X-ray phase-contrast in vivo microtomography probes new aspects of Xenopus gastrulation" were published May 16 in the journal Nature.

The team X-rayed an embryo during gastrulation, the period when its hundreds of cells start to organize into differentiated tissues that eventually form the nervous system, muscles and internal organs. Studies of African clawed frog embryos can provide clues to the evolution of vertebrates and how human genes turn on or off to create diseases.

Until now, however, it has been difficult to study these embryos. Classical absorption imaging requires a contrast agent and large X-ray dose that can harm living organisms. Researchers from the German synchrotron ANKA proposed a new method of nondestructive analysis using X-ray diffraction. The work was done at the APS outside Chicago because the APS's high-energy X-rays were required to prevent blurring of the image and damage to the sensitive embryos.

"To obtain the best possible results, a highly coherent high-energy X-ray source with high flux is necessary," said Xianghui Xiao, a scientist at the APS who collaborated on the work. "The APS is one of only a few X-ray lightsources in the world with this capability. The upgrade of the APS will further improve our ability to study the real-time movement of molecules in living organisms."

In the experiment, Xiao and his colleagues took regular 15-second exposures separated by periods of 10 minutes over the course of two hours of different gastrulas. The resulting 13 time-lapse scans provided a detailed portrait of the transfiguration of the frog cells.

"The motivation of the experiment was to be able to look at the process of how different larger structures develop at the cellular level in real time," Xiao said. "What we're doing is actually a kind of four-dimensional imaging."

The scientists discovered new morphological structures and clarified the process for redistributing fluid in the embryo. They also were able to locate the areas of the embryo that were driving the migration of tissues and cells during gastrulation

"X-ray diffraction enables high-resolution imaging of soft tissues," said Ralf Hofmann, one author of the study and a physicist at the Karlsruhe Institute of Technology in Germany. "In our work, we did not only mange to resolve individual cells and parts of their structure, but we could also analyze single cell migration, as well as the movement of cellular networks."


'/>"/>

Contact: Jared Sagoff
jsagoff@anl.gov
630-252-5549
DOE/Argonne National Laboratory
Source:Eurekalert

Related biology news :

1. Dental X-rays linked to common brain tumor
2. Stanford-SLAC team uses X-ray imaging to observe running batteries in action
3. Speed and power of X-ray laser helps unlock molecular mysteries
4. Low-cost carbon capture gets X-rayed
5. X-rays reveal the self-defence mechanisms of bacteria
6. Study provides recipe for supercharging atoms with X-ray laser
7. X-ray laser helps slay parasite that causes sleeping sickness
8. Scientists reassemble the backbone of life with a particle acceleratorynchrotron X-rays
9. X-rays reveal uptake of nanoparticles by soya bean crops
10. Improved X-ray microscopic imaging
11. A dual look at photosystem II using the worlds most powerful X-ray laser
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/2/2016)... Va. , Feb. 2, 2016   ... award from the U.S. Army Research Office and ... the range and sensitivity of the company,s ... Past Accounting Mission and, more generally, defense-related DNA ... DNA phenotyping capabilities (predicting appearance and ancestry from ...
(Date:2/1/2016)... Rising sales of consumer electronics ... intuitive gesture control market size ... consumer electronics coupled with new technological advancements to drive ... through 2020   --> ... advancements to drive global touchfree intuitive gesture control market ...
(Date:2/1/2016)... , Feb. 1, 2016  Wocket® smart wallet ( www.wocketwallet.com ) ... television personality, Joey Fatone . Las Vegas ... fans. --> Las Vegas , where Joey ... --> The new video ad was filmed at the Consumer Electronics ... at the Wocket booth to meet and greet fans. ...
Breaking Biology News(10 mins):
(Date:2/11/2016)... ... February 11, 2016 , ... Global Stem ... clinic in Quito, Ecuador. The new facility will provide advanced protocols and state-of-the-art ... around the world. , The new GSCG clinic is headed by four ...
(Date:2/10/2016)... -- The Maryland House of Delegates and House Speaker ... Maryland School of Medicine Dean E. Albert Reece ... System President and CEO Robert Chrencik , MBA, ... given to the public by the leader of the ... and Mr. Chrencik for their contributions to our statewide ...
(Date:2/10/2016)... New York, New York (PRWEB) , ... ... ... Regeneron Pharmaceuticals Inc. (NASDAQ: REGN) today announced that it has joined the ... vaccines and immunotherapies for infectious diseases and cancer. , The Human ...
(Date:2/10/2016)... , Feb. 10, 2016  Matchbook, Inc., ... for fast growing biotech companies, announced today the ... Procurement Strategic Advisor. Jim brings nearly 25 years ... and procurement, having spent nearly two decades in ... Chain/Logistics and Procurement at Genzyme and, most recently ...
Breaking Biology Technology: