Navigation Links
New Scripps Research and GNF study helps explain how we can sense temperatures
Date:4/23/2010

rature and postulated the existence of a 'temperature-sensor domain'.

Point by Point

To gain insight into how these ion channels achieve their temperature sensitivity, in the new study the scientists conducted studies of TRPV1, which was not only the first TRP to be discovered but is also the best understood. A previous study in the lab had focused on a related, warm-activated ion channel, TPRV3, but since the biophysics of this molecule is complicated, the team was unable to tease apart its mechanisms.

Using mutagenesis techniques, for the new study the scientists first generated some 8,500 mutants of TRPV1. Then, working with the high throughput equipment available at GNF, the team performed an unbiased screen of these compounds to identify mutations of interest.

"We were looking for mutations in these proteins that would only change the temperature sensitivity of these channels, but would not affect any of the other activation mechanisms," said Grandl. "We were looking for single-point mutations [changes of a single amino acid] where the channel still functioned normally in response to capsacin (the active ingredient in chili peppers) or pH, but not to temperature."

Indeed, the team found a number of these mutations that affected the molecule's sensitivity to temperature, but not to other cues. Interestingly, the mutations were clustered in one area of the protein, the outer pore region, which provides further support to the existence of the predicted 'temperature-sensor domain'.

Next, with these mutant versions of TRPV1 in hand, the scientists examined what had changed in the molecule to disrupt temperature sensitivity.

In findings new to the field, the team discovered that TRPV1 has two ways of opening its channelfor a brief time, opening for only for a millisecond before returning to its closed resting state, and for a relatively long time, opening for about 10 milliseconds. The team foun
'/>"/>

Contact: Keith McKeown
daphne@san.rr.com
858-784-8134
Scripps Research Institute
Source:Eurekalert

Page: 1 2 3 4

Related biology news :

1. Scripps Research scientists reveal how genetic mutations may cause type 1 diabetes
2. New nano-tool synthesized at Scripps Research Institute
3. Scripps Research scientists solve mystery of fragile stem cells
4. Scripps research team reveals how an old drug could have a new use for treating river blindness
5. Scripps Research scientists find two compounds that lay the foundation for a new class of AIDS drug
6. Scripps Research scientists create new way to screen libraries of 10 million or more compounds
7. Scripps Research team wins global race to achieve landmark synthesis of perplexing natural product
8. Scripps Florida scientists show lifeless prions capable of evolutionary change and adaptation
9. Scripps research team develops technique to determine ethnic origin of stem cell lines
10. Scripps Research scientists crack mystery of proteins dual function
11. Scripps Research team uncovers chemical basis for extra quality control in protein production
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/17/2015)... 17, 2015  Crossmatch™, a leading provider of ... U.are.U ® 4500 fingerprint readers have been ... Inc., a San Jose, CA ... security, improve accountability and reduce fraud at the ... identity confirmation for employee sign in and transaction ...
(Date:6/16/2015)... June 16, 2015 Fingerprint Cards has ... sensors in the company,s portfolio from one of its module ... to mainly take place during the third quarter 2015. The ... Asia . Jörgen Lantto, CEO of FPC, ... the   growing interest from smartphone OEMs in integrating touch ...
(Date:6/15/2015)... A new report [ 1 ] ... consumers using mobile banking applications want their mobile devices to ... instead of having to prove who they are with passwords ...   -   The Fusion of Financial Services, Mobile and ... channel used by Gen X and Gen Y to access ...
Breaking Biology News(10 mins):Togo's Eateries Improve Security and Accountability with Crossmatch Fingerprint Readers 2Togo's Eateries Improve Security and Accountability with Crossmatch Fingerprint Readers 3FPC Receives Order for Touch Fingerprint Sensors of 375 MSEK 2One in Four US Consumers Would Share Their DNA With Their Bank to Secure Financial and Personal Information, Telstra Report Finds 2One in Four US Consumers Would Share Their DNA With Their Bank to Secure Financial and Personal Information, Telstra Report Finds 3One in Four US Consumers Would Share Their DNA With Their Bank to Secure Financial and Personal Information, Telstra Report Finds 4
... (MERS) is prevalent in camels throughout Saudi Arabia and has ... study to be published on February 25 in mBio ... Microbiology. "Our study shows the MERS coronavirus (MERS-CoV) is ... University, New York. "Adult camels were more likely to have ...
... Scientists are revealing how microbes living on floating ... and the potential harm they pose to invertebrates, humans ... delves deeper into the largely unexplored world of the ... on ocean plastic that was first discovered last year. ...
... , AMHERST, Mass. Like a kindergarten or a busy ... are common gathering places where pollinators such as bees and ... might be as benign as the sniffles or as debilitating ... how pathogens of pollinators are transmitted at flowers," postdoctoral researcher ...
Cached Biology News:MERS virus widespread in Saudi Arabian camels 2AGU: Uncovering the secret world of the Plastisphere 2As hubs for bees and pollinators, flowers may be crucial in disease transmission 2
(Date:7/2/2015)... (PRWEB) , ... July 02, 2015 , ... Sleepless nights ... an individual’s quality of sleep, launched its Indiegogo campaign on June 23. Sound sleepers ... voice command technology makes it effortless to use, while the integration of the Bluetooth ...
(Date:7/1/2015)... ... 2015 , ... It is now possible to separate two common disorders: post-traumatic ... tomography (SPECT) according to researchers from Amen Clinics, UCLA, Thomas Jefferson University, and ... is believed to be the largest brain imaging study ever, will be published on ...
(Date:7/1/2015)... , July 1, 2015   Decision ... frequently cited advantages of Otezla are its oral ... profile, as reported by surveyed rheumatologists. Otezla was ... for the treatment of active psoriatic arthritis in ... current standard of care includes conventional oral treatments ...
(Date:7/1/2015)... India , July 1, 2015  Strand Life ... generation sequencing technology to empower cancer care, today announced ... chief executive officer. In his expanded role as now ... strategy, business expansion and worldwide operations effective immediately.  Dr. ... chairman and CEO since its founding in 2000, will ...
Breaking Biology Technology:Luzi Smart Lamp Recently Launched Indiegogo Campaign 2Luzi Smart Lamp Recently Launched Indiegogo Campaign 3Brain Scans Can Tell Post-Traumatic Stress Disorder and Traumatic Brain Injury Apart According to New Research by Amen Clinics 2Brain Scans Can Tell Post-Traumatic Stress Disorder and Traumatic Brain Injury Apart According to New Research by Amen Clinics 3Brain Scans Can Tell Post-Traumatic Stress Disorder and Traumatic Brain Injury Apart According to New Research by Amen Clinics 4Surveyed Rheumatologists Report That Otezla's Oral Administration is its Single Greatest Advantage Over Currently Available Treatment Options 2Surveyed Rheumatologists Report That Otezla's Oral Administration is its Single Greatest Advantage Over Currently Available Treatment Options 3Strand Life Sciences Names Scott A. Storrer CEO as Company Continues Global Expansion with Focus on Precision Medicine Product 2Strand Life Sciences Names Scott A. Storrer CEO as Company Continues Global Expansion with Focus on Precision Medicine Product 3
... Pitney Bowes, a mail and document management solutions company ... Firstlogic in a $50.3 million buyout of La ... , ,Company representatives said employment ramifications of the deal ... ,The transaction is expected to close in the third ...
... -- Esker Software has added fax reception to its pay-per-use, ... be able to receive faxes directly into their inboxes. , ... of the most common desktop applications to automate faxing of ... , ,"By adding the flexibility of inbound faxing, we have ...
... Startup entrepreneurs throughout the Upper Midwest will have the chance ... the Wisconsin Early Stage Symposium . , ,The event, ... run from Oct. 19-20. It is being organized by the ... Network and the Wisconsin Entrepreneurs' Network. , ,"When you think ...
Cached Biology Technology:Pitney Bowes to acquire Firstlogic 2Conference will focus on financing of early-stage ventures 2Conference will focus on financing of early-stage ventures 3
...
cysteine and glycine-rich protein 2,...
... agent and DNA-topoisomersae II inhibitor (IC50 = 59.2 ... C 29 H 32 O 13 ... Store at: RT ... Soluble to 100 mM ...
HSPBP1 Antibody...
Biology Products: