Navigation Links
New, more direct pathways from outside the cell-to-cell nuclei discovered

A team of Brooklyn College researchers has shattered a long-held belief that no direct pathway exists between material outside of a cell and the cell nucleus. (The cell is the smallest metabolically functional unit of life.)

It was already known that material outside of a cell can migrate into a cell. This occurs through processes known as endocytosis and phagocytosis, in which extracellular material is captured by a pinched-in segment of the cell membrane. This extracellular material then becomes trapped inside the resulting membrane-bound intracellular compartment, which is known as an endosome or phagosome.

It was also already known that material can migrate out of an endosome or phagosome and eventually enter the cell nucleus. But the Brooklyn College team has discovered that a phagosome and its contents can enter the cell's nucleus, where genetic information is stored and processed.

The team's discovery of the existence of direct pathways between extracellular material and cell nuclei will be published on August 10, 2007 in Cell Motility and the Cytoskeleton. The team was led by Ray Gavin and funded by the National Science Foundation (NSF).

Gavin says that these newly discovered direct pathways mean that "internalized material does not necessarily have to exit the phagosome before entering the nucleus." Therefore, his discovery means that "one less step is needed for extracellular material to get into the nucleus, and so it is far easier than previously thought for this material to get into the nucleus."

Eve Barak, an NSF program director, describes Gavin's discovery as "an amazing and potentially paradigm-changing observation," and predicts that it "will have an enormous impact on how scientists think about how cellular functions are regulated."

Gavin has only observed direct pathways from the extracellular environment to the nucleus in the one-celled protozoan Tetrahymena thermophila. But Gavin says that "other discoveries that were based on this protozoan were later shown to be almost global in their occurrence in the biological world." Therefore, these direct pathways may operate in other organisms as well.

The discovery of the pathways in the protozoan resulted from an observation made by Gavin about 10 years ago while simply watching cells through a microscope. "I am a patient and constant observer of living things," he says. "And I watch them all the time with no agenda in mind. Some people are window gazers. They stand in front of the window and just look. I do the same with cells."

During one of Gavin's agenda-less cell-watching sessions, he noticed phagosomes clustered around a cell's nucleus. "It made me wonder why the phagosomes were positioned in that way," Gavin said. Thereafter, Gavin periodically looked for this intriguing behavior again, and occasionally glimpsed it again.

But Gavin remained unable to systematically search for phagosome clusters around nuclei until he received NSF funding in 2006 to purchase a confocal microscope, which provides three dimensional views of the cell. Just like a person would eventually find a worm hidden in an apple by repeatedly slicing through the apple, Gavin found the intruiging phagosome behavior he sought by using the confocal microscope to repeatedly obtain views that sliced clear through the cell.

Specifically, the team tracked phagosomes carry extracellular material into the cell nuceli by introducing fluorescent latex beads into the area outside of the cell. They then observed the cells phagocytose (eat) the beads, which gradually moved to the nucleus. The arrival of the phagosomes and their loads at the nucleus was marked by the illumination of the nucleus by the beads. Similarly, the researchers also labeled the cellular membrane with a fluorescent dye, and then observed the pinched off, internalized membrane move to the nucleus.

"Biologists may now study the kinds of external molecules that can gain entry to the nucleus through these newly defined pathways and how these materials influence the nucleic material and its processes," said Gavin.

Contact: Lily Whiteman
National Science Foundation

Related biology news :

1. Combination therapy boosts effectiveness of telomere-directed cancer cell death
2. BRCA1 causes ovarian cancer through indirect, biochemical route
3. Poplar trees redirect resources in response to simulated attack
4. Weizmann Institute scientists develop a new approach for directing treatment to metastasized prostate cancer in the bones.
5. Cerebral navigation: How do nerve fibers know what direction to grow in?
6. Stem cells bring fast direct improvement, without differentiation, in acute renal failure
7. Locusts built-in surface analysis ability directs them to fly overland
8. Cells direct membrane traffic by channel width
9. SLU researchers uncover direct evidence on how HIV invades healthy cells
10. Penn study finds direct role for glial cells in brain cross-talk
11. Navigating the brain for sense of direction as paradigm for higher cognitive functions
Post Your Comments:
(Date:4/26/2016)... -- Research and Markets has announced the ...  report to their offering.  , ,     (Logo: ... forecast the global multimodal biometrics market to grow ... 2016-2020.  Multimodal biometrics is being implemented ... healthcare, BFSI, transportation, automotive, and government for controlling ...
(Date:4/15/2016)... April 15, 2016  A new partnership announced ... accurate underwriting decisions in a fraction of the ... priced and high-value life insurance policies to consumers ... With Force Diagnostics, rapid testing (A1C, Cotinine ... readings (blood pressure, weight, pulse, BMI, and activity ...
(Date:4/14/2016)... , April 14, 2016 ... Malware Detection, today announced the appointment of Eyal ... new role. Goldwerger,s leadership appointment comes at ... heels of the deployment of its platform at several ... biometric technology, which discerns unique cognitive and physiological factors, ...
Breaking Biology News(10 mins):
(Date:6/27/2016)... ... June 27, 2016 , ... Rolf K. Hoffmann, former senior ... the University of North Carolina Kenan-Flagler Business School effective June 27. ... Kenan-Flagler, with a focus on the school’s international efforts, leading classes and participating ...
(Date:6/24/2016)... ... June 24, 2016 , ... While the majority of commercial spectrophotometers and ... and the 6000i models are higher end machines that use the more unconventional z-dimension ... light beam from the bottom of the cuvette holder. , FireflySci has developed ...
(Date:6/23/2016)... , June 23, 2016 /PRNewswire/ - FACIT has ... Ontario biotechnology company, Propellon Therapeutics Inc. ... and commercialization of a portfolio of first-in-class WDR5 ... targets such as WDR5 represent an exciting class ... in precision medicine for cancer patients. Substantial advances ...
(Date:6/23/2016)... June, 23, 2016  The Biodesign Challenge (BDC), a ... ways to harness living systems and biotechnology, announced its ... in New York City . ... students, showcased projects at MoMA,s Celeste Bartos Theater during ... , MoMA,s senior curator of architecture and design, and ...
Breaking Biology Technology: