Navigation Links
Nature: Microscope looks into cells of living fish
Date:5/16/2012

This release is available in German.

Microscopes provide valuable insights in the structure and dynamics of cells, in particular when the latter remain in their natural environment. However, this is very difficult especially for higher organisms. Researchers of Karlsruhe Institute of Technology (KIT), the Max Planck Institute for Polymer Research, Mainz, and the American National Institutes of Health (NIH) have now developed a new method to visualize cell structures of an eighth of a micrometer in size in living fish larvae. It is published in the Nature Methods magazine (DOI:10.1038/nmeth.2025). "The zebrafish is perfectly suited for genetic studies of cells, as its larvae are completely transparent," explains Marina Mione, KIT. To visualize certain structures, these are colored mostly by genetic engineering methods using a fluorescent dye. Mione studied parts of the cellular skeleton of fish, the so-called microtubuli. The thread-shaped microtubuli have a length of about 100 m and a diameter of about 20 nm, corresponding to a hundred thousandth of a human hair. "Microtubuli exist everywhere in the cell and are required for its division and motion."

In the new microscopy method, the object is not illuminated completely, but only at a certain spot with special light. Scattered light is minimized and the illuminated detail is represented sharply. A series of images taken at variable illumination is then processed by a computer. In this way, an overall image is obtained. Smart illumination even allows to adjust the depth of field, to image various depth levels, and to combine them into a three-dimensional image on the computer. "Meanwhile, it is possible to reach resolutions of 145 nm in the plane and 400 nm in-between," says Marina Mione. The images are taken within a few seconds, such that movement of the cells does not cause any unsharpness.
'/>"/>

Contact: Monika Landgraf
presse@kit.edu
49-721-608-47414
Helmholtz Association of German Research Centres
Source:Eurekalert  

Page: 1 2

Related biology news :

1. Changing the course of nature: Are fisheries directing the evolution of fish populations?
2. Commentary in Nature: Can economy bear what oil prices have in store?
3. Nature: Video reveals wave character of particles
4. Microscope reveals how bacteria breathe toxic metals
5. Computational microscope peers into the working ribosome
6. Compact microscope a marvel
7. New microscope reveals ultrastructure of cells
8. International conference puts food safety under the microscope
9. New microscope decodes complex eye circuitry
10. Brandeis researchers use lasers, custom microscope to show gene splicing process in real time
11. Big size multitouch display turned into a microscope
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Nature: Microscope looks into cells of living fish
(Date:7/11/2014)... with other group members to reduce aggression and gain ... strategy for grooming activities shows a certain pattern across ... scientific journal Biology Letters . , Grooming between ... without ulterior motives. To be groomed has hygienic benefits ... another individual can provide access to infants, mating opportunities ...
(Date:7/11/2014)... kidney injury related to the use of hydroxyethyl starch ... molecules, according to a report in Anesthesia & ... Society (IARS). , The "total mass of HES molecules" ... renal proximal tubule cells (PTCs), concludes the laboratory study ... Wrzburg, Austria. Other factorssuch as differences in the ...
(Date:7/11/2014)... Evidence exists that cannabinoid receptor type 1 ... Ca2+ influx, and reduce neurotransmitter release. However, ... 1 can increase extracellular Ca2+ influx and ... his team, Tongji Hospital Affiliated to Tongji ... Technology, China used whole cell voltage-clamp and ...
Breaking Biology News(10 mins):Baboons groom early in the day to get benefits later 2In lab studies, hydroxyethyl starch has direct harmful effects on kidney cells 2
... ways to automate the production of DNA and proteins, making ... complex carbohydrates, it,s been a different story. Until now, ... study has been slow and tedious. In what may change ... Georgia has created a method for the rapid chemical synthesis ...
... MADISON The "mineral-breathing" bacteria found in many ... or anaerobic, environments contain microbes sometimes described as "mineral-breathing" ... the same way we use oxygen. According to a ... Nature Geoscience , this bacterial respiration may be accelerated ...
... microbicide based on a new type of anti-HIV drug found ... similar to HIV, according to a study reported at the ... is the first of a gel with an integrase inhibitor, ... for the treatment of HIV but just one of the ...
Cached Biology News:New method for producing 'libraries' of important carbohydrate molecules 2New method for producing 'libraries' of important carbohydrate molecules 3Organic solids in soil may speed up bacterial breathing 2Vaginal gel with integrase inhibitor shows promise in monkeys 2Vaginal gel with integrase inhibitor shows promise in monkeys 3Vaginal gel with integrase inhibitor shows promise in monkeys 4Vaginal gel with integrase inhibitor shows promise in monkeys 5Vaginal gel with integrase inhibitor shows promise in monkeys 6
(Date:7/10/2014)... CA (PRWEB) July 10, 2014 OMICS ... Bioanalytical techniques during 18-20 August, 2014 at Double Tree ... review the recent developments in Analytical & Bioanalytical research ... globe. , Speaking on this occasion, Dr. Srinubabu Gedela, ... Analytica Acta conference is a remarkable one in bringing ...
(Date:7/10/2014)... -- Kainos Capital, a firm specializing in acquiring and managing ... acquired the Slim-Fast brand from Unilever. Unilever will retain ... transaction were not disclosed. Slim-Fast is a ... ready-to-drink shakes, powders, bars and snacks to retail customers ... the United Kingdom and Ireland.  ...
(Date:7/10/2014)... fight against terrorism with the creation of a sensor ... use of light and special glass fibres. , Published ... , the researchers describe a novel optical fibre sensor ... 6.3 ppm (parts per million). It requires an analysis ... detection has involved looking for metals that encase them ...
(Date:7/10/2014)... power our laptops and electric vehicles could store more ... the help of a sponge-like silicon material. , ... traditionally used in one of the battery,s electrodes, as ... capacity of graphite. A paper describing the material,s performance ... Nature Communications . , "Silicon has long been ...
Breaking Biology Technology:A Critical Review of Analytical Techniques for Improved Living Standards 2A Critical Review of Analytical Techniques for Improved Living Standards 3A Critical Review of Analytical Techniques for Improved Living Standards 4Kainos Capital Acquires Slim-Fast From Unilever 2Detecting trace amounts of explosives with light 2Silicon sponge improves lithium-ion battery performance 2Silicon sponge improves lithium-ion battery performance 3
... Md. - Engineers at the University of Idaho have ... upcoming NASA missions that will study the Earth and ... a grant technical officer Pen-Shu Yeh, a senior engineer ... engineering team at the University of Idaho,s ...
... sensitive microscopy developed by researchers at Harvard University could ... scientists to track the location of minuscule metabolites and ... of any kind of fluorescent labeling. , The technique, ... the vibrations in chemical bonds between atoms. SRS microscopy ...
... iPods to those who test their knowledge at www.synbioproject.org ... Among the 75 people Esquire magazine recently chose as ... an emerging discipline that combines science and engineering in order to ... as synthetic biology. , , ...
Cached Biology Technology:New one-of-a-kind technology will fly on 2 NASA missions 2New one-of-a-kind technology will fly on 2 NASA missions 3New label-free method tracks molecules and drugs in live cells 2Synthetic Biology Importance in the 21st Century 2Synthetic Biology Importance in the 21st Century 3
... Digital Sight DS-L2 imaging controller is a ... DS-L1, and allows users to comfortably observe, ... DS-L2 controller features an 8.4 inch LCD ... variety of Nikon cameras including the DS-Fi1 ...
... a premium quality recombinant form of Thermus ... suitable for a wide range of PCR ... reproducible performance, each preparation is extensively tested ... NovaTaq DNA Polymerase possesses an integral 5′→3′ ...
Mouse polyclonal antibody raised against a partial recombinant TSEN2. NCBI Entrez Gene ID = 80746...
Mouse polyclonal antibody raised against a partial recombinant NCLN. NCBI Entrez Gene ID = 56926...
Biology Products: