Navigation Links
Nature: Microscope looks into cells of living fish
Date:5/16/2012

This release is available in German.

Microscopes provide valuable insights in the structure and dynamics of cells, in particular when the latter remain in their natural environment. However, this is very difficult especially for higher organisms. Researchers of Karlsruhe Institute of Technology (KIT), the Max Planck Institute for Polymer Research, Mainz, and the American National Institutes of Health (NIH) have now developed a new method to visualize cell structures of an eighth of a micrometer in size in living fish larvae. It is published in the Nature Methods magazine (DOI:10.1038/nmeth.2025). "The zebrafish is perfectly suited for genetic studies of cells, as its larvae are completely transparent," explains Marina Mione, KIT. To visualize certain structures, these are colored mostly by genetic engineering methods using a fluorescent dye. Mione studied parts of the cellular skeleton of fish, the so-called microtubuli. The thread-shaped microtubuli have a length of about 100 m and a diameter of about 20 nm, corresponding to a hundred thousandth of a human hair. "Microtubuli exist everywhere in the cell and are required for its division and motion."

In the new microscopy method, the object is not illuminated completely, but only at a certain spot with special light. Scattered light is minimized and the illuminated detail is represented sharply. A series of images taken at variable illumination is then processed by a computer. In this way, an overall image is obtained. Smart illumination even allows to adjust the depth of field, to image various depth levels, and to combine them into a three-dimensional image on the computer. "Meanwhile, it is possible to reach resolutions of 145 nm in the plane and 400 nm in-between," says Marina Mione. The images are taken within a few seconds, such that movement of the cells does not cause any unsharpness.

Based on a series of images, videos of the movement of the microtubuli are obtained. In the experiment, it was observed over a period of 60 minutes how the early stage of the fish's lateral line develops about 45 m below the skin of the fish. Via this organ, the fish perceives movement stimuli in water. Such images of living organisms also provide valuable findings regarding the development of vertebrates on the cellular level.

The tropical zebrafish living in freshwater has several advantages as a genetic model organism. It is sufficiently small for easy cultivation and large enough to easily distinguish individual organs. It has a short generation cycle and produces many offsprings. As a vertebrate, it has a number of microbiological properties in common with human beings.


'/>"/>

Contact: Monika Landgraf
presse@kit.edu
49-721-608-47414
Helmholtz Association of German Research Centres
Source:Eurekalert  

Related biology news :

1. Changing the course of nature: Are fisheries directing the evolution of fish populations?
2. Commentary in Nature: Can economy bear what oil prices have in store?
3. Nature: Video reveals wave character of particles
4. Microscope reveals how bacteria breathe toxic metals
5. Computational microscope peers into the working ribosome
6. Compact microscope a marvel
7. New microscope reveals ultrastructure of cells
8. International conference puts food safety under the microscope
9. New microscope decodes complex eye circuitry
10. Brandeis researchers use lasers, custom microscope to show gene splicing process in real time
11. Big size multitouch display turned into a microscope
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Nature: Microscope looks into cells of living fish
(Date:3/20/2017)... 2017 PMD Healthcare announces the release of ... Management System (WMS), a remote, real-time lung health monitoring ... Healthcare is a Medical Device, Digital Health, and Chronic ... creating innovative solutions that empower people to improve their ... PMD developed the first ever personal spirometer, Spiro PD, ...
(Date:3/9/2017)... , Australia , March 9, 2017 ... data at the prestigious World Lung Imaging Workshop at ... Andreas Fouras , was invited to deliver the latest ... medicine. This globally recognised event brings together leaders at ... the latest developments in lung imaging. ...
(Date:3/7/2017)... LAKE CITY , March 7, 2017   ... that help top global companies identify the best talent, ... as Chief Sales Officer (CSO) and Diana ... Kucer,s appointments round out a seasoned executive team poised to ... and beyond, building on a year of record bookings ...
Breaking Biology News(10 mins):
(Date:3/27/2017)... 27, 2017  The global market for clinical ... to a new report from Kalorama Information.  In ... performed to evaluate disease progression, monitor drug treatment ... reasons.  The healthcare market research firm,s report, ... an overview of the medical laboratory industry and ...
(Date:3/27/2017)... ... ... PMG Research is pleased to announce its participation in the 2nd ... Boston on April 3-4, 2017. The CTC conference focuses on how the industry is ... closer to the patient. Clinical Trial Collaborations also will present an inaugural conference on ...
(Date:3/27/2017)... 2017 DarioHealth Corp. (NASDAQ: DRIO), a leading ... data solutions, today announced that it is now offering ... U.S. consumers who want to have their DarioHealth products ... strategic alliance agreements with partners across the U.S. who ... if approved, will supply and bill the customer,s insurance ...
(Date:3/24/2017)... and ROCKVILLE, Md. , March 24, ... Maxwell Biotech Venture Fund (MBVF), today announced positive results ... to the standard drug therapy regimen in patients with ... molecule drug discovered by scientists at Sequella, Inc. ( ... of Health. A total of 140 ...
Breaking Biology Technology: