Navigation Links
National Xenopus resource at the MBL innovates new way to study proteins
Date:7/17/2014

WOODS HOLE, Mass. Proteomics, the study of large groups of proteins, can enhance our understanding of a wide range of organisms, with applications in medicine and developmental biology. Such analyses traditionally require a complete genome for the organism being studied in order to obtain a reference set of proteins. However, many organisms that hold potential for proteomic analysis do not yet have completely sequenced and well-interpreted genomes because the costs, in terms of both time and money, can be prohibitive. Xenopus laevis, the African clawed frog, is one such species.

A collaborative study between scientists at Harvard Medical School and the MBL's National Xenopus Resource (NXR), published last week in Current Biology, has found a work-around. Instead of relying on DNA, researchers used mRNA sequences to more efficiently create a reference database that can be used for proteomic analysis of Xenopus.

"The mRNA is the product of a gene, so it's simpler to sequence because the different [coding sequences] have already been spliced together," said Marko Horb, the MBL scientist who contributed the mRNA sequences for the project. Horb is director of the NXR, a collaborative center that facilitates frog research through breeding programs and development of new experimental techniques. Harvard Medical School researchers Martin Wuhr and Robert Freeman, Jr. (co-first authors on the paper), along with Leon Peshkin, have all taught at the NXR Bioinformatics Workshop at the MBL.

The researchers used their reference database to identify over 11,000 proteins from an unfertilized Xenopus egg and estimate the abundance of these proteins. The method outperformed comparison proteomic analyses based on a preliminary, unpublished Xenopus genome and other protein reference databases.

Their cost-effective solution opens the door for further proteomics experiments in frogs. "Xenopus is
'/>"/>

Contact: Diana Kenney
dkenney@mbl.edu
508-289-7139
Marine Biological Laboratory
Source:Eurekalert  

Page: 1 2

Related biology news :

1. Story tips From the Department of Energys Oak Ridge National Laboratory, March 2012
2. Ultracold matter technology from CU and SRI International licensed to Boulders ColdQuanta
3. International Polar Year conference: From knowledge to action
4. ASBMB wins National Science Foundation grant to enhance K-12 science education
5. President, CEO Leppävuori, VTT: Finland should create a national bioeconomy strategy
6. Russia declares Land of the Leopard National Park
7. Large international study finds memory in adults impacted by versions of 4 genes
8. International team uncovers new genes that shape brain size, intelligence
9. As deadly cat disease spreads nationally, MU veterinarian finds effective treatment
10. Southampton researchers lead 2 international projects to help people out of poverty
11. Ohio state hosts national energy conference
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
National Xenopus resource at the MBL innovates new way to study proteins
(Date:12/17/2014)... 16, 2014  Automation is fundamentally transforming the ... than at international borders. Over the past decade, ... allowed veteran travelers to self process through border ... Kiosks at an increasing number of airports, seaports, ... According to Maxine Most , Principal ...
(Date:12/11/2014)... -- That blood pressure plays a role in human health has ... term for high blood pressure – was first described as ... that,s used in measuring blood pressure was invented in 1896. ... hypertension, its triggers and its effects. In fact, recent findings ... best ways to treat it. For example, a ...
(Date:12/10/2014)... WINSTON-SALEM, N.C. , Dec. 9, 2014  Wake Forest ... medical education building for its School of Medicine. Funding ... a larger capital campaign that will be publicly launched ... will be located in the former 60 series R.J. ... Forest Innovation Quarter. Construction will begin immediately with plans ...
Breaking Biology News(10 mins):Automated Border Control (ABC) Transforms the Global Travel Experience With More Than 2500 ABC eGates and APC Kiosks Deployed In Airports, Seaports, and Land Borders Worldwide 2Research points to need for new approaches to treatment of high blood pressure 2Research points to need for new approaches to treatment of high blood pressure 3Research points to need for new approaches to treatment of high blood pressure 4Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 2Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 3Wake Forest Baptist to Build New Medical Education Facility In Wake Forest Innovation Quarter 4
... have been uncovered from old experiments by researchers at ... Children,s Hospital. The finding suggests that useful information about ... discarded data. "We,ve devised a fairly simple way ... candidate disease genes for further genetic study," said Atul ...
... biologists will gather in Auckland from 14-16 November to share ... the tropics. This major meeting of a global marine biology ... hosted by the National Institute of Water & Atmospheric Research ... Census of Marine Life is a ten-year initiative to assess ...
... Case Western Reserve Universitys School of Medicine have ... discovery with a natural product derived from the ... broad set of applications in various joint, skin ... syndrome., The publication revealed that Progrado, an extract ...
Cached Biology News:Stanford/Packard researchers find disease genes hidden in discarded data 2Stanford/Packard researchers find disease genes hidden in discarded data 3Natural product discovery by Cleveland medical researchers blocks tissue destruction 2
(Date:12/15/2014)... NEW YORK , Dec. 15, 2014  GlassesOff ... Yuval Bar-Gil as an independent director of ... was founder of AeroScout and served as its CEO ... Decker in 2012, a $240 million transaction. Recognized as ... under Mr. Bar-Gil,s leadership, AeroScout provided Wi-Fi -based RFID ...
(Date:12/15/2014)... Denmark , Dec. 15, 2014  Ascendis ... innovative TransCon technology to address significant unmet medical ... its ongoing Phase 2 pediatric study to evaluate ... hormone deficiency, or GHD.  This interim analysis consists ... anticipated total enrollment in the study, completing all ...
(Date:12/13/2014)... , Dec. 12, 2014 /CNW/ - MaRS and Virgin ... today that they have joined forces to support Canadian entrepreneurs ... , Virgin Group Founder was at the MaRS Centre to ... new, national impact venture fund. This fund has $1 million ... Vancouver -based Mindset Social Innovation Foundation, founded ...
(Date:12/13/2014)... The DNA Microarray market market was ... expected to grow at a CAGR of 15.2% ... TOC of the DNA Microarray for an in-depth ... help of various tables and figures. , ... consists of instrument, consumables, and services, where the ...
Breaking Biology Technology:AeroScout Founder Joins GlassesOff's Board of Directors 2AeroScout Founder Joins GlassesOff's Board of Directors 3Ascendis Pharma A/S Announces Positive Six-Month Interim Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 2Ascendis Pharma A/S Announces Positive Six-Month Interim Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 3Ascendis Pharma A/S Announces Positive Six-Month Interim Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 4MaRS and Virgin Unite partner to invest in business as a force for good 2MaRS and Virgin Unite partner to invest in business as a force for good 3DNA Microarray Market Is Expected to Grow at a CAGR of 15.2% from 2014 to 2019 - A Report by MicroMarket Monitor 2DNA Microarray Market Is Expected to Grow at a CAGR of 15.2% from 2014 to 2019 - A Report by MicroMarket Monitor 3DNA Microarray Market Is Expected to Grow at a CAGR of 15.2% from 2014 to 2019 - A Report by MicroMarket Monitor 4
... The Board of Molecular Vision today,announces that discussions ... have now ceased. Acrongenomics has been unable to,complete ... the Board of,Molecular Vision believes that the best ... discussions to a conclusion. Acrongenomics remains a,significant shareholder ...
... Martek,Biosciences Corporation announced today that the United ... entered a Final Judgment and Order,of Dismissal approving ... behalf of certain purchasers of Martek common stock. ... laws and arose out of,allegations, among other things, ...
... GAITHERSBURG, Md., April 7, 2008 MaxCyte, ... clinical scale,non-viral cell loading systems, announces that ... MaxCyte, will present on Tuesday,April 8, 2008, ... Conference in,Madrid, Spain. Dr. Recupero,s presentation will ...
Cached Biology Technology:Martek Announces Final Judgment and Order of Dismissal in Class Action 2
...
... The Antibody Validation Service enables early ... further development by providing quantitative data ... limit of each antibody. Furthermore quantitative ... the off-target activity of your antibodies., ...
... faster than western blotting. Get results sooner and ... on gel detection. Eliminates the need for a ... All reagents are ready-to-use. No reagents to ... Only 2 reagents required. Guarantees a simple to ...
... filter paper sandwiches are composed of a ... of thick filter paper that are preassembled ... sandwich. These 7 x 8.5 cm membrane ... Ready Gel-sized gels and are supplied as ...
Biology Products: