Navigation Links
Nanoneedle is small in size, but huge in applications
Date:4/28/2009

CHAMPAIGN, Ill. Researchers at the University of Illinois have developed a membrane-penetrating nanoneedle for the targeted delivery of one or more molecules into the cytoplasm or the nucleus of living cells. In addition to ferrying tiny amounts of cargo, the nanoneedle can also be used as an electrochemical probe and as an optical biosensor.

"Nanoneedle-based delivery is a powerful new tool for studying biological processes and biophysical properties at the molecular level inside living cells," said

Min-Feng Yu, a professor of mechanical science and engineering and corresponding author of a paper accepted for publication in Nano Letters, and posted on the journal's Web site.

In the paper, Yu and collaborators describe how they deliver, detect and track individual fluorescent quantum dots in a cell's cytoplasm and nucleus. The quantum dots can be used for studying molecular mechanics and physical properties inside cells.

To create a nanoneedle, the researchers begin with a rigid but resilient boron-nitride nanotube. The nanotube is then attached to one end of a glass pipette for easy handling, and coated with a thin layer of gold. Molecular cargo is then attached to the gold surface via "linker" molecules. When placed in a cell's cytoplasm or nucleus, the bonds with the linker molecules break, freeing the cargo.

With a diameter of approximately 50 nanometers, the nanoneedle introduces minimal intrusiveness in penetrating cell membranes and accessing the interiors of live cells.

The delivery process can be precisely controlled, monitored and recorded goals that have not been achieved in prior studies.

"The nanoneedle provides a mechanism by which we can quantitatively examine biological processes occurring within a cell's nucleus or cytoplasm," said Yang Xiang, a professor of molecular and integrative physiology and a co-author of the paper. "By studying how individual proteins and molecules of DNA or RNA mobilize, we can better understand how the system functions as a whole."

The ability to deliver a small number of molecules or nanoparticles into living cells with spatial and temporal precision may make feasible numerous new strategies for biological studies at the single-molecule level, which would otherwise be technically challenging or even impossible, the researchers report.

"Combined with molecular targeting strategies using quantum dots and magnetic nanoparticles as molecular probes, the nanoneedle delivery method can potentially enable the simultaneous observation and manipulation of individual molecules," said Ning Wang, a professor of mechanical science and engineering and a co-author of the paper.

Beyond delivery, the nanoneedle-based approach can also be extended in many ways for single-cell studies, said Yu, who also is a researcher at the Center for Nanoscale Chemical-Electrical-Mechanical Manufacturing Systems. "Nanoneedles can be used as electrochemical probes and as optical biosensors to study cellular environments, stimulate certain types of biological sequences, and examine the effect of nanoparticles on cellular physiology."


'/>"/>

Contact: James E. Kloeppel, Physical Sciences Editor
kloeppel@illinois.edu
217-244-1073
University of Illinois at Urbana-Champaign
Source:Eurekalert

Related biology news :

1. Social parasites of the smaller kind
2. Small animal imaging facility is big boon to research
3. Handbook of small grain insects available now
4. New molecular clock from LLNL and CDC indicates smallpox evolved earlier than believed
5. 2007 ozone hole smaller than usual
6. Cilia: small organelles, big decisions
7. Research shows loggerhead sea turtles threatened by small-scale fishing operations
8. Small-scale fishing in Mexico rivals industrial fisheries in accidental turtle deaths
9. Sweet potato shines as new promise for small enterprise and hunger relief in developing countries
10. Identification of a novel class of (not-so) small RNAs
11. Small RNA plays parallel roles in bacterial metabolism
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:1/15/2016)... 2016 Recent publicized breaches in cyber security ... ways to ensure data security and user authentication in ... Android that ties a user,s mobile number ... a hardware authorization token. Customer service agents who employ ... their KodeKey enabled device to verify their identity.  Companies ...
(Date:1/13/2016)... 2016 http://www.researchandmarkets.com/research/7h6hnn/india_biometrics ... the  "India Biometrics Authentication & Identification ...  report to their offering.  ... the addition of the  "India Biometrics ... & Forecast (2015-2020)"  report to ...
(Date:1/11/2016)... 11, 2016  higi, the leading retail and ... locations, web and mobile, today announced it has ... existing investors. --> ... further innovate higi,s health platform – its network ... – including expanding services and programs to retail ...
Breaking Biology News(10 mins):
(Date:2/11/2016)... 11, 2016 Non-profit Consortium Aims to ... to Support Research and Discovery --> ... an ambitious plan to sequence 100,000 individuals. It is intended ... at least 7 of North and East Asian countries. ... phase, the project will focus on creating phased reference genomes ...
(Date:2/11/2016)... 2016  Bioethics International, a not-for-profit organization focused on the ... and made accessible to patients around the world, today announced ... the publication of the Good Pharma Scorecard an ... featured as one of BMJ Open ,s ,Most Popular ... are most frequently read. Ed Sucksmith , assistant ...
(Date:2/11/2016)... CRUZ, Calif. , Feb. 11, 2016  Dovetail ... applications to its beta program for a planned metagenomic ... will present the company,s metagenomic genome assembly method in ... Advances in Genome Biology & Technology conference in ... assembly of these highly complex datasets is difficult. Using ...
(Date:2/11/2016)... ... , ... Global Stem Cells Group, a world ... Cells Network (GSCN) to distribute exosome injection and other biological products to stem ... Dominican Republic, Colombia, Argentina, Nicaragua, Panama, El Salvador, Venezuela, Peru, Ecuador, Paraguay, Puerto ...
Breaking Biology Technology: