Navigation Links
Nanocrystals reveal activity within cells
Date:6/16/2009

Researchers at the U.S. Department of Energy's (DOE) Lawrence Berkeley National Laboratory have created bright, stable and bio-friendly nanocrystals that act as individual investigators of activity within a cell.

These ideal light emitting probes represent a significant step in scrutinizing the behaviors of proteins and other components in complex systems such as a living cell.

Labeling a given cellular component and tracking it through a typical biological environment is fraught with issues: the probe can randomly turn on and off, competes with light emitting from the cell, and often requires such intense laser excitation, it eventually destroys the probe, muddling anything you'd be interested in seeing.

"The nanoparticles we've designed can be used to study biomolecules one at a time," said Bruce Cohen, a staff scientist in the Biological Nanostructures Facility at Berkeley Lab's nanoscience research center, the Molecular Foundry. "These single-molecule probes will allow us to track proteins in a cell or around its surface, and to look for changes in activity when we add drugs or other bioactive compounds."

Molecular Foundry post-doctoral researchers Shiwei Wu and Gang Han, led by Cohen, Imaging and Manipulation of Nanostructures staff scientist Jim Schuck and Inorganic Nanostructures Facility Director Delia Milliron, worked to develop nanocrystals containing rare earth elements that absorb low-energy infrared light and transform it into visible light through a series of energy transfers when they are struck by a continuous wave, near-infrared laser. Biological tissues are more transparent to near-infrared light, making these nanocrystals well suited for imaging living systems with minimal damage or light scatter.

"Rare earths have been known to show phosphorescent behavior, like how the old-style television screen glows green after you shut it off. These nanocrystals draw on this property, and are a million times more efficient than traditional dyes," said Schuck. "No probe with ideal single-molecule imaging properties had been identified to dateour results show a single nanocrystal is stable and bright enough that you can go out to lunch, come back, and the intensity remains constant."

To study how these probes might behave in a real biological system, the Molecular Foundry team incubated the nanocrystals with embryonic mouse fibroblasts, cells crucial to the development of connective tissue, allowing the nanocrystals to be taken up into the interior of the cell. Live-cell imaging using the same near-infrared laser showed similarly strong luminescence from the nanocrystals within the mouse cell, without any measurable background signal.

"While these types of particles have existed in one form or another for some time, our discovery of the unprecedented 'single-molecule' properties these individual nanocrystals possess opens a wide range of applications that were previously inaccessible," Schuck adds.


'/>"/>

Contact: Aditi Risbud
ASRisbud@lbl.gov
510-486-4861
DOE/Lawrence Berkeley National Laboratory
Source:Eurekalert  

Related biology news :

1. Childhood obesity indicates greater risk of school absenteeism, Penn study reveals
2. Study begins to reveal clues to the cause and progression of sepsis
3. Comet probes reveal evidence of origin of life, scientists claim
4. Structure of 450 million year old protein reveals evolutions steps
5. UF scientists reveal how dietary restriction cleans cells
6. Neural stem cell study reveals mechanism that may play role in cancer
7. New method reveals substances on surfaces of any kind
8. Study reveals predation-evolution link
9. IDEMA Reveals Program Highlights for DISKCON USA 2007
10. Study reveals possible genetic risk for fetal alcohol disorders
11. IDEMA Reveals Program Highlights for DISKCON USA 2007
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Nanocrystals reveal activity within cells
(Date:4/5/2017)... -- The Allen Institute for Cell Science today announces the ... and dynamic digital window into the human cell. The ... of deep learning to create predictive models of cell ... growing suite of powerful tools. The Allen Cell Explorer ... available resources created and shared by the Allen Institute ...
(Date:3/30/2017)... 30, 2017  On April 6-7, 2017, Sequencing.com will ... hackathon at Microsoft,s headquarters in ... focus on developing health and wellness apps that provide ... the Genome is the first hackathon for personal ... largest companies in the genomics, tech and health industries ...
(Date:3/27/2017)... March 27, 2017  Catholic Health Services (CHS) ... Systems Society (HIMSS) Analytics for achieving Stage 6 ... sm . In addition, CHS previously earned a ... using an electronic medical record (EMR). ... level of EMR usage in an outpatient setting.  ...
Breaking Biology News(10 mins):
(Date:10/12/2017)... ... 12, 2017 , ... BioMedGPS announces expanded coverage of SmartTRAK ... module, US Hemostats & Sealants. , SmartTRAK’s US Market for Hemostats and Sealants ... sealants and biologic sealants used in surgical applications. BioMedGPS estimates the market will ...
(Date:10/11/2017)... ... October 11, 2017 , ... At ... Purple announced Dr. Suneel I. Sheikh, the co-founder, CEO and chief research scientist ... has been selected for membership in ARCS Alumni Hall of Fame . ...
(Date:10/11/2017)... ... 2017 , ... ComplianceOnline’s Medical Device Summit is back for its 4th year. ... San Francisco, CA. The Summit brings together current and former FDA office bearers, regulators, ... government officials from around the world to address key issues in device compliance, quality ...
(Date:10/11/2017)... Netherlands and LAGUNA HILLS, Calif. ... Institute of Cancer Research, London (ICR) ... MMprofiler™ with SKY92, SkylineDx,s prognostic tool to risk-stratify patients with ... known as MUK nine . The University of ... which is partly funded by Myeloma UK, and ICR will ...
Breaking Biology Technology: