Navigation Links
NIST team advances in translating language of nanopores
Date:6/24/2010

National Institute of Standards and Technology (NIST) scientists have moved a step closer to developing the means for a rapid diagnostic blood test that can scan for thousands of disease markers and other chemical indicators of health. The team reports* it has learned how to decode the electrical signals generated by a nanoporea "gate" less than 2 nanometers wide in an artificial cell membrane.

Nanopores are not new themselves; for more than a decade, scientists have sought to use a nanopore-based electrical detector to characterize single-stranded DNA for genetic sequencing applications. More recently, NIST scientists turned their attention to using nanopores to identify, quantify and characterize each of the more than 20,000 proteins the body producesa capability that would provide a snapshot of a patient's overall health at a given moment. But while nanopores permit molecules to enter into them one at a time, determining what specific individual molecule has just passed through has not been easy.

To address this problem, members of the NIST team that previously developed a method to distinguish both the size and concentration of each type of molecule the nanopore admits** have now answered the question of just how these single molecules interact with the nanopore. Their new theoretical model describes the physics and chemistry of how the nanopore, in effect, parses a molecule, an understanding that will advance the use of nanopores in the medical field.

"This work brings us one step closer to realizing these nanopores as a powerful diagnostic tool for medical science," says Joseph Reiner, who performed the work with Joseph Robertson, and John Kasianowicz, all of NIST's Semiconductor Electronics Division. "It adds to the 'Rosetta Stone' that will allow us to read what molecules have just passed through a nanopore."

Using their new methods, the team was able to model the interaction of a particular type of large molecule through a nanopore's opening with great accuracy. The molecules were polyethylene glycol (PEG), a well-understood polymer that forms chains of varying length.

"PEG chains can be very long, but each link is very small," Kasianowicz says. "It was a good test because we wanted to see if the nanopore could differentiate between two nearly identical large molecules that differ in length by only a few atoms."

The team's device was able to distinguish among different-sized PEG chains easily, and the model they have developed to describe the PEG-nanopore interactions is encouraging them to think that with further effort, the minuscule sensors can be customized to measure many different molecules quickly. "We could conceivably build an array of many nanopores, each one created to measure a specific substance," Kasianowicz says. "Because each nanopore is so small, an array with one for every protein in the body would still be tiny."


'/>"/>

Contact: Chad Boutin
boutin@nist.gov
301-975-4261
National Institute of Standards and Technology (NIST)
Source:Eurekalert  

Related biology news :

1. Restoring sight, advances in fertility treatments and better visibility for pilots at FIO
2. Cardiologists and heart surgeons meet for Controversies and Advances conference
3. Lockheed Martin Advances Biometrics Portfolio Through Cooperation Agreement With Cognitec
4. Genetic Engineering & Biotechnology News reports on advances in miRNA
5. Prototype terahertz imager promises biochem advances
6. Science expands Science Signaling, featuring research related to medical advances, and more
7. Analysis of RNA role in spreading disease advances study of damaging plant infections
8. Iowa State-ConocoPhillips collaboration advances 26 research projects in first year
9. Brown to host conference on advances in neurotechnology
10. Latest advances in interventional cardiology for congenital heart disease presented
11. Advances in the field of schizophrenia research: New genetic factors identified
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
NIST team advances in translating language of nanopores
(Date:4/15/2016)...  A new partnership announced today will help ... in a fraction of the time it takes ... life insurance policies to consumers without requiring inconvenient ... Diagnostics, rapid testing (A1C, Cotinine and HIV) and ... weight, pulse, BMI, and activity data) available at ...
(Date:4/13/2016)... , April 13, 2016  IMPOWER physicians supporting ... are setting a new clinical standard in telehealth thanks ... By leveraging the higi platform, IMPOWER patients can routinely ... pulse and body mass index, and, when they opt ... and convenient visit to a local retail location at ...
(Date:3/29/2016)... , March 29, 2016 ... "Company") LegacyXChange "LEGX" and SelectaDNA/CSI Protect are pleased to ... ink used in a variety of writing instruments, ensuring ... of originally created collectibles from athletes on LegacyXChange will ... analysis of the DNA. Bill Bollander ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... HOUSTON , June 23, 2016 ... agreement with the Cy-Fair Sports Association to serve ... of the agreement, Houston Methodist Willowbrook will provide ... education and connectivity with association coaches, volunteers, athletes ... partner with the Cy-Fair Sports Association and to ...
(Date:6/23/2016)... 23, 2016  The Biodesign Challenge (BDC), a university ... to harness living systems and biotechnology, announced its winning ... New York City . ... showcased projects at MoMA,s Celeste Bartos Theater during the ... MoMA,s senior curator of architecture and design, and ...
(Date:6/23/2016)... ... June 23, 2016 , ... STACS DNA Inc., the sample tracking ... Arkansas State Crime Laboratory, has joined STACS DNA as a Field Application Specialist. ... Jocelyn Tremblay, President and COO of STACS DNA. “In further expanding our capacity as ...
(Date:6/23/2016)... June 23, 2016  Blueprint Bio, a company dedicated ... the medical community, has closed its Series A funding ... . "We have received a commitment from ... we need to meet our current goals," stated ... the runway to complete validation on the current projects ...
Breaking Biology Technology: